FastQCFastQC Report
Fri 27 May 2016
SRR939517_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939517_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2590044
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129420.49968263087422454No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103370.399105188946597No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67810.2618102240734134No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39160.15119434264437207No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26730.10320287995107419No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA190200.032.1313441
GGTATCA127100.031.8453041
GTACATG142950.028.0690651
TACATGG148550.026.620662
TATCAAC237250.025.6194362
ATCAACG238500.025.532173
TCAACGC240750.025.3715884
CAACGCA239700.025.2295675
AACGCAG244750.024.7072126
ACATGGG152800.024.6764073
GAGTACT129100.022.69725612-13
ACGCAGA269400.021.9908337
CGCAGAG269850.021.9239378
CATGGGG57250.021.6564664
AGTACTT134300.021.35459312-13
CATGGGA97200.021.3247994
GCAGAGT266550.021.0443129
GTATAGG7900.020.9250451
GTACTTT142500.020.66498614-15
AGAGTAC228050.019.75592210-11
Produced by FastQC (version 0.11.5)