FastQCFastQC Report
Fri 27 May 2016
SRR939517_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939517_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2590044
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179050.6913009972031363No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161960.625317562172689No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112520.43443277411503434No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA56870.21957155940207965No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43970.16976545572198773No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34020.13134911993773077No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA141450.033.616071
GTATCAA206400.029.6068461
GTACATG171400.023.7038561
ATCAACG255800.023.5419453
TATCAAC259750.023.337532
TCAACGC259700.023.2066544
TACATGG173700.023.056682
CAACGCA261350.023.0420135
AACGCAG267300.022.4936546
GAGTACT142450.022.35180312-13
ACATGGG174700.021.8594633
GTACTTT150800.021.23983414-15
AGTACTT145750.020.82170712-13
GTCGGGA6250.020.4749892
CGCGGAA4650.020.37860182-83
ATTCGCG4550.020.30601780-81
ACGCAGA300750.019.9288527
TCGCGGA4650.019.86913782-83
CGCAGAG302450.019.7541738
CATGGGA112050.019.3664934
Produced by FastQC (version 0.11.5)