##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939517_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2590044 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.997703900010965 31.0 31.0 31.0 30.0 34.0 2 31.245466872377456 31.0 31.0 34.0 30.0 34.0 3 31.228913485639627 31.0 31.0 34.0 28.0 34.0 4 35.17287891634273 35.0 35.0 37.0 33.0 37.0 5 34.80056941117603 35.0 35.0 37.0 32.0 37.0 6 34.76651091641686 35.0 35.0 37.0 32.0 37.0 7 34.632127871186746 35.0 35.0 37.0 30.0 37.0 8 34.65021366432385 35.0 35.0 37.0 30.0 37.0 9 35.99026734680955 37.0 35.0 39.0 30.0 39.0 10-11 35.84806802509919 37.0 35.0 39.0 30.0 39.0 12-13 35.810297045146726 37.0 35.0 39.0 30.0 39.0 14-15 36.63853587043309 38.0 35.5 40.0 30.5 41.0 16-17 36.65891042777652 38.0 35.5 40.0 30.5 41.0 18-19 36.647954436295294 38.0 35.0 40.0 30.0 41.0 20-21 36.560279863971424 38.0 35.0 40.0 30.0 41.0 22-23 36.41857551454724 38.0 35.0 40.0 30.0 41.0 24-25 36.196678318978364 38.0 34.0 40.0 29.5 41.0 26-27 35.82463058542635 38.0 34.0 40.0 28.0 41.0 28-29 35.53183671783182 38.0 34.0 40.0 27.0 41.0 30-31 35.284220461119574 38.0 34.0 40.0 27.0 41.0 32-33 35.04390253601869 37.0 33.0 40.0 27.0 41.0 34-35 34.96713395602546 37.5 33.0 40.0 26.0 41.0 36-37 34.93070484516865 38.0 33.0 40.0 25.5 41.0 38-39 34.88427648333388 38.0 33.0 40.0 26.0 41.0 40-41 34.64337227475672 38.0 33.0 40.0 25.0 41.0 42-43 34.31213987098289 37.0 33.0 40.0 24.0 41.0 44-45 34.09102876244573 37.0 33.0 40.0 24.0 41.0 46-47 33.70581715986292 37.0 32.5 40.0 23.0 41.0 48-49 33.3539532533038 37.0 32.0 40.0 21.5 41.0 50-51 33.06965151943365 37.0 31.5 40.0 20.0 41.0 52-53 32.68625687440059 36.0 31.0 39.5 18.5 41.0 54-55 32.45649707109223 36.0 31.0 39.0 18.0 40.5 56-57 32.06015399738383 36.0 30.5 39.0 15.0 40.0 58-59 31.63484133860274 35.0 30.0 39.0 13.0 40.0 60-61 31.33303044272607 35.0 29.0 38.5 11.5 40.0 62-63 30.95469111721654 35.0 29.0 38.0 8.0 40.0 64-65 30.516282155824378 34.0 28.5 38.0 7.5 40.0 66-67 28.83865640892587 33.5 25.0 37.0 4.5 39.5 68-69 25.214199835987344 29.5 15.5 35.0 2.0 38.0 70-71 24.62623395587102 29.5 14.0 35.0 2.0 38.0 72-73 26.436573085244884 31.0 21.0 35.0 2.0 38.0 74-75 27.03335387352493 31.5 23.5 35.0 2.0 37.5 76-77 27.074912047826214 31.5 25.0 34.5 2.0 36.5 78-79 27.903280021497704 32.5 26.0 35.0 2.0 37.0 80-81 27.76252777945085 33.0 26.0 35.0 2.0 36.5 82-83 27.361960839275316 33.0 26.0 35.0 2.0 36.0 84-85 27.030584808597844 32.0 25.5 35.0 2.0 36.0 86-87 26.58502133554488 32.0 25.0 34.5 2.0 35.0 88-89 26.09085714373964 32.0 24.0 34.0 2.0 35.0 90-91 25.64207094551289 32.0 22.5 34.0 2.0 35.0 92-93 25.09338412783721 31.0 19.5 34.0 2.0 35.0 94-95 23.739241109417446 31.0 7.5 34.0 2.0 35.0 96-97 22.94247375720258 30.0 2.0 34.0 2.0 35.0 98-99 22.61590247115493 30.0 2.0 34.0 2.0 35.0 100-101 21.382292154110125 28.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.4073811236793148 1101 2 0.279500913700371 1101 3 0.3865939243856822 1101 4 0.2911336609764561 1101 5 0.5104191554848967 1101 6 0.5323412734929818 1101 7 0.5275134639772148 1101 8 0.5539505519263628 1101 9 0.700611420653992 1101 10-11 0.8252925296286904 1101 12-13 0.5953896982252687 1101 14-15 0.6319390811186807 1101 16-17 0.5897335550059779 1101 18-19 0.777202067894315 1101 20-21 0.7791799379684079 1101 22-23 0.7532091738458888 1101 24-25 0.69805660116063 1101 26-27 0.7238998566657315 1101 28-29 0.744776699618086 1101 30-31 0.7359343906946734 1101 32-33 0.8135025656725432 1101 34-35 0.7732889444331548 1101 36-37 0.7714331889222663 1101 38-39 0.5092464190062742 1101 40-41 0.7563043442355664 1101 42-43 0.7954056693075415 1101 44-45 0.5756416399164834 1101 46-47 0.7034284181300308 1101 48-49 0.5119234517045541 1101 50-51 0.5033893555833444 1101 52-53 0.7020936891756264 1101 54-55 0.48536083869081637 1101 56-57 0.45896880359650183 1101 58-59 0.4781753822832684 1101 60-61 0.24909447003178187 1101 62-63 0.19531933298784665 1101 64-65 0.022545881693474712 1101 66-67 -2.8378402185639047 1101 68-69 -7.581630032034074 1101 70-71 -10.983643959621087 1101 72-73 -10.844910540353542 1101 74-75 -9.343990633796189 1101 76-77 -4.587538124926748 1101 78-79 -1.5867646423150958 1101 80-81 -0.9578635543861616 1101 82-83 -0.7197079868050196 1101 84-85 -0.818492370634921 1101 86-87 -0.6905189123437445 1101 88-89 -0.6225231422663775 1101 90-91 -0.6583115609858297 1101 92-93 -1.1120676726424463 1101 94-95 -4.98452559615178 1101 96-97 -1.9385805448740179 1101 98-99 -1.2368861631476449 1101 100-101 -0.805764120477857 1102 1 0.3462062845747269 1102 2 0.1832991174816314 1102 3 0.38445931518602805 1102 4 0.24234543426695865 1102 5 0.4300155422978662 1102 6 0.467345924848793 1102 7 0.41766279151912045 1102 8 0.4733299331058305 1102 9 0.6568151880792854 1102 10-11 0.5829620793290289 1102 12-13 0.5932775676968092 1102 14-15 0.5821518503872198 1102 16-17 0.586158149438873 1102 18-19 0.5675806778824537 1102 20-21 0.6910951034632973 1102 22-23 0.642426068815162 1102 24-25 0.6621023988595027 1102 26-27 0.6541956586874278 1102 28-29 0.5470335582064934 1102 30-31 0.45219563435309595 1102 32-33 0.39107256944204494 1102 34-35 0.6475666009798005 1102 36-37 0.8188536749457214 1102 38-39 0.7190562840305006 1102 40-41 0.6380613463416509 1102 42-43 0.7601746750240395 1102 44-45 0.5249120341232114 1102 46-47 0.5860020011956308 1102 48-49 0.5298627281680055 1102 50-51 0.4416620599058163 1102 52-53 0.5029447662497972 1102 54-55 0.39458029277034967 1102 56-57 0.21674555926717787 1102 58-59 0.13519841081734896 1102 60-61 -0.008747127959662038 1102 62-63 0.10149335940201354 1102 64-65 -0.08599194991015224 1102 66-67 -3.013815792893954 1102 68-69 -7.626795734423386 1102 70-71 -10.819051238560647 1102 72-73 -9.204384695069049 1102 74-75 -7.768301489611076 1102 76-77 -4.272951715185528 1102 78-79 -1.468985050873588 1102 80-81 -0.9159235413139513 1102 82-83 -0.6456995555741933 1102 84-85 -0.7685568671299805 1102 86-87 -0.6939075720270402 1102 88-89 -0.6170115450011693 1102 90-91 -0.7197302072165037 1102 92-93 -0.9987825185943819 1102 94-95 -4.515239674370644 1102 96-97 -1.4802183927314587 1102 98-99 -0.8551429940699968 1102 100-101 -0.7118689817498733 1103 1 0.35326700775463493 1103 2 0.24329323164453598 1103 3 0.34206195682464013 1103 4 0.23121973730496137 1103 5 0.35322385902650666 1103 6 0.44203418380448767 1103 7 0.5206019038431506 1103 8 0.373317409792719 1103 9 0.6219542706979695 1103 10-11 0.637558321350518 1103 12-13 0.6259077023902648 1103 14-15 0.8752744622676545 1103 16-17 0.7708007133104644 1103 18-19 0.6321514446274037 1103 20-21 0.6853515494575078 1103 22-23 0.7434028151959851 1103 24-25 0.7312846385201723 1103 26-27 0.7792393191834108 1103 28-29 0.8949291553193746 1103 30-31 0.8250728397259408 1103 32-33 0.9373813319069768 1103 34-35 0.7584101091548376 1103 36-37 0.8218526062016096 1103 38-39 0.9522807177708188 1103 40-41 1.0438414857118303 1103 42-43 1.0093213667261267 1103 44-45 0.8179846368002757 1103 46-47 0.7479155922562697 1103 48-49 0.7772544150174596 1103 50-51 0.6410483935492124 1103 52-53 0.7519219789829492 1103 54-55 0.744936726913636 1103 56-57 0.7115072603101176 1103 58-59 0.7756277359260366 1103 60-61 0.6084988790185726 1103 62-63 0.5754202537512363 1103 64-65 0.41157747072872297 1103 66-67 -2.726896483494034 1103 68-69 -7.532423744956414 1103 70-71 -11.383059116601109 1103 72-73 -9.061353450543496 1103 74-75 -7.339658859460773 1103 76-77 -3.748535697207668 1103 78-79 -0.8684918868855469 1103 80-81 -0.20560686478752643 1103 82-83 -0.029806918910086466 1103 84-85 -0.08364786767065624 1103 86-87 0.03798042128553547 1103 88-89 0.0632368728649304 1103 90-91 -0.004779844802865085 1103 92-93 -0.038599450089702 1103 94-95 0.7939138589266612 1103 96-97 0.9488973136764578 1103 98-99 0.7158603490422486 1103 100-101 0.5243303091880627 1104 1 0.43226781464246855 1104 2 0.21506016305596276 1104 3 0.3781976989445788 1104 4 0.14099359483220297 1104 5 0.4399034229940213 1104 6 0.5023397592643946 1104 7 0.5251139278817902 1104 8 0.3936642938949291 1104 9 0.6521776148125156 1104 10-11 0.7300165786759223 1104 12-13 0.6683247550260418 1104 14-15 0.7768270970318412 1104 16-17 0.7357082020014332 1104 18-19 0.7532867201551667 1104 20-21 0.8269861132678926 1104 22-23 0.7766569174732254 1104 24-25 0.6990257934917494 1104 26-27 0.9952629549150771 1104 28-29 1.1298673984689884 1104 30-31 1.022141661056743 1104 32-33 0.801516500016966 1104 34-35 0.8326492860981887 1104 36-37 0.8476135128983486 1104 38-39 0.870303040831125 1104 40-41 1.111764342790174 1104 42-43 1.0874762736568186 1104 44-45 0.9468844602360633 1104 46-47 0.887728534794455 1104 48-49 0.682136940728121 1104 50-51 0.7845101944659802 1104 52-53 0.7249895136902964 1104 54-55 0.7818618521915468 1104 56-57 0.6740959471763439 1104 58-59 0.8376747576639758 1104 60-61 0.6584859566256966 1104 62-63 0.8042267826483496 1104 64-65 0.5450549756685703 1104 66-67 -2.7329897830511136 1104 68-69 -7.787898078025329 1104 70-71 -9.171228059532645 1104 72-73 -8.509993719682942 1104 74-75 -7.202775052384034 1104 76-77 -3.651589133781542 1104 78-79 -0.6380040728274885 1104 80-81 0.16052503108343785 1104 82-83 0.2948699047693282 1104 84-85 0.2733920524267788 1104 86-87 0.3818194867724962 1104 88-89 0.3424026777400222 1104 90-91 0.3346748075154622 1104 92-93 0.576729530852667 1104 94-95 1.505634929285538 1104 96-97 1.4487698820351973 1104 98-99 1.0190064229827094 1104 100-101 0.9215387975094558 1105 1 0.49285830493781546 1105 2 0.3475300768651337 1105 3 0.4817551485826357 1105 4 0.3434589245587176 1105 5 0.45093292735897705 1105 6 0.5184822788978707 1105 7 0.6065317695344774 1105 8 0.5175000124624773 1105 9 0.6972376360705681 1105 10-11 0.88150917613806 1105 12-13 0.8482902988443399 1105 14-15 0.9309102049391385 1105 16-17 0.8514751794789817 1105 18-19 0.8278259190993111 1105 20-21 0.9885057618551443 1105 22-23 1.058473473567794 1105 24-25 1.0923375665242503 1105 26-27 1.0578797608673298 1105 28-29 1.199477449196145 1105 30-31 0.9739647154662805 1105 32-33 1.107555651105308 1105 34-35 1.2705677095767527 1105 36-37 1.1732722858754343 1105 38-39 0.9472627361659391 1105 40-41 1.0984163504672537 1105 42-43 1.2640969483733357 1105 44-45 1.2076369046603546 1105 46-47 1.1379061050291526 1105 48-49 0.9968165838749172 1105 50-51 1.02618076515909 1105 52-53 1.2911803928770453 1105 54-55 1.2440532451985504 1105 56-57 1.254010108969272 1105 58-59 1.461306459366135 1105 60-61 1.405615867513145 1105 62-63 1.441438199532886 1105 64-65 1.1976709252537319 1105 66-67 2.0962052822594686 1105 68-69 -4.896715888445804 1105 70-71 -4.969683324427134 1105 72-73 -1.4036921172689993 1105 74-75 1.250348924383232 1105 76-77 1.2971484803797502 1105 78-79 1.2681028320099763 1105 80-81 1.2373222685439558 1105 82-83 1.3878738893950207 1105 84-85 1.4059488623082466 1105 86-87 1.5672783735960287 1105 88-89 1.5160411800043576 1105 90-91 1.4316873457371422 1105 92-93 1.487025078247548 1105 94-95 2.3148640477815547 1105 96-97 2.2338078896216054 1105 98-99 1.9882212854854195 1105 100-101 1.9351411812324386 1106 1 0.25825266403532154 1106 2 0.1623261420360116 1106 3 0.165169934696074 1106 4 0.11758268518922677 1106 5 0.3002842257357585 1106 6 0.2551107179251062 1106 7 0.4162968004389356 1106 8 0.3219246399755562 1106 9 0.3165967876635989 1106 10-11 0.46756192898241267 1106 12-13 0.4142032298247358 1106 14-15 0.44383880557388977 1106 16-17 0.3667981457014946 1106 18-19 0.38054306701885565 1106 20-21 0.5311624638071919 1106 22-23 0.42190701653656504 1106 24-25 0.615474266572491 1106 26-27 0.6578530426247227 1106 28-29 0.6751000275173737 1106 30-31 0.6085840940886627 1106 32-33 0.6595648196812007 1106 34-35 0.7658146748387082 1106 36-37 0.7887232184178217 1106 38-39 0.7495607213724682 1106 40-41 1.0035325153285442 1106 42-43 1.0514164920646891 1106 44-45 0.8305575871762372 1106 46-47 0.8011221160712481 1106 48-49 0.8011157330016516 1106 50-51 0.8419703811735886 1106 52-53 1.1191457392082143 1106 54-55 1.0806198104007976 1106 56-57 1.0325732083719537 1106 58-59 1.1435129751558577 1106 60-61 1.030214823446432 1106 62-63 1.1862102940051287 1106 64-65 1.019276897159557 1106 66-67 1.6825192681236807 1106 68-69 -5.005930220673626 1106 70-71 -5.809515031949996 1106 72-73 -1.9612363970771476 1106 74-75 0.9052357666763697 1106 76-77 1.2545967043197273 1106 78-79 0.8729778836616404 1106 80-81 0.9614596587233244 1106 82-83 0.9849703628033382 1106 84-85 1.0705856997336909 1106 86-87 1.2395385638472227 1106 88-89 1.1375258921937181 1106 90-91 1.0897235873565911 1106 92-93 1.0269423490493779 1106 94-95 1.851468040157659 1106 96-97 2.0458699321933906 1106 98-99 1.8089218659311257 1106 100-101 1.6539695376170158 1107 1 -0.04762184530712332 1107 2 -0.041256989398767985 1107 3 0.04968780988664534 1107 4 0.040547928846585535 1107 5 0.10157384421514593 1107 6 0.20022435087515333 1107 7 0.0714199343513684 1107 8 0.06487581714405621 1107 9 0.09871293277038262 1107 10-11 0.1337634159190273 1107 12-13 0.2943412780385799 1107 14-15 0.07353259007756208 1107 16-17 -0.02231002003679805 1107 18-19 0.24417297585976883 1107 20-21 0.15449926783439594 1107 22-23 0.16869929352276358 1107 24-25 0.24079778216194114 1107 26-27 0.3264595615212542 1107 28-29 0.28000783193607504 1107 30-31 0.1308083272279248 1107 32-33 0.15602039219300678 1107 34-35 0.36533911778364825 1107 36-37 0.32692828379372685 1107 38-39 0.22081770341299034 1107 40-41 0.28588028828756507 1107 42-43 0.30947306983641454 1107 44-45 0.21127706350364406 1107 46-47 -0.009541541171167012 1107 48-49 0.04711413221237848 1107 50-51 0.11286858830450086 1107 52-53 0.15164662736324175 1107 54-55 0.1331401225249138 1107 56-57 0.17824559075999602 1107 58-59 0.24230579186225754 1107 60-61 0.23240992559729534 1107 62-63 0.29299495433268774 1107 64-65 0.33442719583038993 1107 66-67 1.2465350744281771 1107 68-69 -5.500382634307847 1107 70-71 -6.1280797312198345 1107 72-73 -2.448593066482136 1107 74-75 0.1410734489281431 1107 76-77 0.05370472606643162 1107 78-79 0.2774196021963107 1107 80-81 0.12523252887046965 1107 82-83 0.1868138027326509 1107 84-85 0.12164877971878241 1107 86-87 0.2746571198201053 1107 88-89 0.272475804222303 1107 90-91 0.3054753736591671 1107 92-93 0.36633698357513467 1107 94-95 1.0232440498180075 1107 96-97 1.079529642328385 1107 98-99 0.6899290771789559 1107 100-101 0.5197161849093668 1108 1 0.06606588104394007 1108 2 -0.05435628154296168 1108 3 -0.019763139892173598 1108 4 -0.03589848668275408 1108 5 0.039223702606271615 1108 6 0.15998983199962424 1108 7 0.20522052039714112 1108 8 0.1648276713843373 1108 9 0.12654520450856666 1108 10-11 0.2224147815364148 1108 12-13 0.16088902593581622 1108 14-15 0.12565754026618947 1108 16-17 0.03095144663640781 1108 18-19 0.08837342312507701 1108 20-21 0.2768387117822968 1108 22-23 0.26575435693562355 1108 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0.5287430593821298 2311 60-61 0.6897657319981789 2311 62-63 0.8981752558539107 2311 64-65 0.8222028545143658 2311 66-67 1.783337778134495 2311 68-69 4.487889443979757 2311 70-71 4.821896610800991 2311 72-73 2.859152506497928 2311 74-75 1.9252404287016667 2311 76-77 1.275356799529117 2311 78-79 0.945565121273507 2311 80-81 0.8865761456485295 2311 82-83 0.7840854229556697 2311 84-85 0.6291890744445112 2311 86-87 0.7339059762367555 2311 88-89 0.7270548170697921 2311 90-91 0.5621560768445697 2311 92-93 0.70290590692575 2311 94-95 1.4806908378905455 2311 96-97 1.8818200887588468 2311 98-99 1.3434979052938552 2311 100-101 1.190337626778117 2312 1 -0.0578670284400431 2312 2 -0.02986938104799819 2312 3 -0.016142108146141254 2312 4 0.02002918157690914 2312 5 0.17057237558800864 2312 6 0.19206104011249892 2312 7 0.1310717748774266 2312 8 0.2068077803335342 2312 9 -1.482410035293448 2312 10-11 -0.8272346182473953 2312 12-13 -1.78492866195176 2312 14-15 -1.8568574295807778 2312 16-17 0.07755051786585199 2312 18-19 -1.824191114338653 2312 20-21 -1.7563942595723177 2312 22-23 -0.6281532531514173 2312 24-25 -1.2182419627395333 2312 26-27 -1.4647122937198134 2312 28-29 -0.027134445269453522 2312 30-31 0.5249448477599614 2312 32-33 0.5360474224047636 2312 34-35 0.7952856393503396 2312 36-37 0.8271066530140629 2312 38-39 0.754793896421063 2312 40-41 0.9563053423192471 2312 42-43 1.0457107977466507 2312 44-45 1.0902239989300426 2312 46-47 1.1539166949242983 2312 48-49 1.1215005636496187 2312 50-51 1.1277644839083862 2312 52-53 1.3623310334745682 2312 54-55 1.3724658114288744 2312 56-57 1.3759253898081134 2312 58-59 1.4714062361866311 2312 60-61 1.5044691506450043 2312 62-63 1.1186985876759863 2312 64-65 1.6565792519083828 2312 66-67 2.8599917298095043 2312 68-69 4.739994948004252 2312 70-71 5.440452489482347 2312 72-73 3.6860051016138833 2312 74-75 2.788863215384815 2312 76-77 1.7021970121814363 2312 78-79 1.5490820320700855 2312 80-81 1.581232452708786 2312 82-83 1.7698887731301909 2312 84-85 1.7476834289726852 2312 86-87 1.821524036622808 2312 88-89 1.8770366315393439 2312 90-91 1.833484191170033 2312 92-93 1.838824115125039 2312 94-95 2.531295812457973 2312 96-97 2.9464933275765866 2312 98-99 2.8940973485099697 2312 100-101 2.8546393334620035 2313 1 -0.2193099178880331 2313 2 -0.16220288660405302 2313 3 -0.19126687601986347 2313 4 -0.11138939077161325 2313 5 -0.07960873906739607 2313 6 -0.10664195431647272 2313 7 -3.6394096250686587 2313 8 -1.1962743511847194 2313 9 -0.8921737532076008 2313 10-11 -0.16152453647904252 2313 12-13 -1.4661874602864415 2313 14-15 -0.4042921534253878 2313 16-17 -0.19185121409569206 2313 18-19 0.19863635219022768 2313 20-21 0.17200999395450367 2313 22-23 -1.2303901361447984 2313 24-25 -2.7598229860420176 2313 26-27 -2.3760531599252275 2313 28-29 -0.5046605501156449 2313 30-31 -0.1328095270715579 2313 32-33 -2.596331478814271 2313 34-35 -2.7155407582744857 2313 36-37 -1.19784668773233 2313 38-39 -0.6537078688618294 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0.003982891853858916 2314 4 -0.06088614100374201 2314 5 -1.4183106889281163 2314 6 -3.032063959887502 2314 7 -0.7840572573806384 2314 8 -0.18224463902130594 2314 9 0.037436738900105127 2314 10-11 0.2537331236880931 2314 12-13 0.23947456385469224 2314 14-15 -2.2771921070001326 2314 16-17 -2.755413191811563 2314 18-19 -0.28879192079026694 2314 20-21 0.05176904687930062 2314 22-23 0.23290118233245494 2314 24-25 -2.0718185756427587 2314 26-27 -0.9082606808165892 2314 28-29 0.03963773214990596 2314 30-31 0.33835412195351466 2314 32-33 0.18897887401767122 2314 34-35 0.02134410709227552 2314 36-37 0.28603608849793716 2314 38-39 -1.1549137648692565 2314 40-41 -3.8184728834872033 2314 42-43 -5.071168234511411 2314 44-45 -1.836790113973187 2314 46-47 -2.665016772817637 2314 48-49 -0.7132151621568283 2314 50-51 -0.5409391451238648 2314 52-53 -2.3796810632996213 2314 54-55 -1.4834878176033826 2314 56-57 -3.5666068682564003 2314 58-59 -4.853412312200465 2314 60-61 -2.1652768170969345 2314 62-63 -1.2097167349046565 2314 64-65 -0.2783844577690431 2314 66-67 -0.06782077019049737 2314 68-69 1.9603155125203813 2314 70-71 1.8565049088371879 2314 72-73 -0.2597892532248274 2314 74-75 0.3226313605461044 2314 76-77 0.19798733476207886 2314 78-79 0.5874630804571801 2314 80-81 0.9387223720636264 2314 82-83 0.7372174021624467 2314 84-85 0.6809818160694618 2314 86-87 0.4700744398486165 2314 88-89 0.5376112283135335 2314 90-91 0.8100003202022883 2314 92-93 0.9099793570605215 2314 94-95 0.7762413769741023 2314 96-97 1.0519792146733629 2314 98-99 0.8493836388325526 2314 100-101 0.8554296560426522 2315 1 -0.2824833722833411 2315 2 -0.2877905381149155 2315 3 -0.2862112390716476 2315 4 -0.13600577982992235 2315 5 -1.0536650907339862 2315 6 -0.27813506598231186 2315 7 -0.034182539547678914 2315 8 -0.2674365126104732 2315 9 0.19863425583012884 2315 10-11 -1.8003695851155186 2315 12-13 -1.3507782578157403 2315 14-15 -0.15708657652609048 2315 16-17 -1.4579103701411356 2315 18-19 -2.874672846298168 2315 20-21 -1.9214352420597578 2315 22-23 -1.371207734146104 2315 24-25 -1.0944241061168043 2315 26-27 -0.2272505228030397 2315 28-29 -0.5567409360216544 2315 30-31 -2.9049906861729013 2315 32-33 -2.39894606954892 2315 34-35 -1.4651118748490362 2315 36-37 -4.333801947619445 2315 38-39 -1.9359493179166662 2315 40-41 -1.401695231116996 2315 42-43 -1.4307737649854602 2315 44-45 -1.1873583983975635 2315 46-47 -1.0508028901991295 2315 48-49 -4.547465726490916 2315 50-51 -3.8413968200674375 2315 52-53 -2.26806086013935 2315 54-55 -3.7654313841948053 2315 56-57 -5.639252466450532 2315 58-59 -6.853824276083081 2315 60-61 -5.710672979625148 2315 62-63 -6.334740436936261 2315 64-65 -6.11561978508281 2315 66-67 -3.8715086934410614 2315 68-69 -1.7435659773216088 2315 70-71 0.7396826740742064 2315 72-73 -0.8543868830216681 2315 74-75 -2.553182973539677 2315 76-77 -4.874943816479455 2315 78-79 -3.7020312762019607 2315 80-81 -3.540230788207257 2315 82-83 -6.856498737792407 2315 84-85 -4.972425068806388 2315 86-87 -5.452861226065604 2315 88-89 -3.796835724282399 2315 90-91 -3.3047367001137395 2315 92-93 -4.375122787409005 2315 94-95 -2.226072957111679 2315 96-97 -0.9353497920986449 2315 98-99 -2.3086180541697026 2315 100-101 -1.5541888541153632 2316 1 -0.3006083028980022 2316 2 -0.23936105212150238 2316 3 -0.8267056327363065 2316 4 -0.3673779442824241 2316 5 -0.45460577089533416 2316 6 -2.1811869107071757 2316 7 -0.4656720114790005 2316 8 -0.2955233275458937 2316 9 0.0792973946378055 2316 10-11 -0.004832450082396633 2316 12-13 -0.6663287148338384 2316 14-15 -0.9857617791312805 2316 16-17 -1.4876058147623823 2316 18-19 -0.27193536341322044 2316 20-21 -4.261616199022342 2316 22-23 -4.22032422750361 2316 24-25 -2.5377980838394834 2316 26-27 -1.852236090652653 2316 28-29 -1.2381532514566516 2316 30-31 -0.27160079607927656 2316 32-33 -1.930123584998725 2316 34-35 -2.406770647006084 2316 36-37 -4.936549977075835 2316 38-39 -2.225589994377458 2316 40-41 -1.784497473651136 2316 42-43 -2.9123444640196112 2316 44-45 -0.7435483106945 2316 46-47 -0.8323569367520633 2316 48-49 -0.4495430310092914 2316 50-51 -0.01625471889436625 2316 52-53 -2.473955653463559 2316 54-55 -0.5726189651443647 2316 56-57 0.20627837764523704 2316 58-59 0.2570385074716661 2316 60-61 -0.9383669810313577 2316 62-63 -0.5452659152325268 2316 64-65 0.6397016078047315 2316 66-67 1.5615030003013075 2316 68-69 4.055290922356445 2316 70-71 4.30214835146014 2316 72-73 2.542796812348943 2316 74-75 1.7532829998903665 2316 76-77 0.28848733476207755 2316 78-79 0.5927581624243956 2316 80-81 0.1853822081292016 2316 82-83 0.2586313365886781 2316 84-85 0.679346570167823 2316 86-87 0.39378755460271364 2316 88-89 0.9376931955266521 2316 90-91 0.9578773693826186 2316 92-93 1.2061801767326514 2316 94-95 1.736458590088855 2316 96-97 1.7559587228700835 2316 98-99 0.6453959339145214 2316 100-101 0.39916736096068206 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 35.0 4 104.0 5 250.0 6 558.0 7 1327.0 8 2851.0 9 4438.0 10 7377.0 11 10773.0 12 14236.0 13 17809.0 14 21790.0 15 27360.0 16 32893.0 17 37123.0 18 33361.0 19 28034.0 20 26903.0 21 27211.0 22 28571.0 23 32587.0 24 39199.0 25 48764.0 26 61077.0 27 76255.0 28 93530.0 29 111664.0 30 130856.0 31 152288.0 32 176962.0 33 204934.0 34 237837.0 35 272939.0 36 285035.0 37 238473.0 38 98607.0 39 6025.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.26253989769969 20.249745600874324 15.53045355453989 21.957260946886098 2 15.97285108062384 21.48461771051476 38.99378622465746 23.548744984203935 3 19.378744144887115 25.70454401546846 30.51222295837445 24.40448888126997 4 13.093291079224908 17.781087888854398 36.414323463230744 32.71129756868995 5 13.298404657843122 38.49396148322033 33.35933035783231 14.84830350110423 6 31.167617229668686 36.71219485074385 17.038552240811352 15.081635678776115 7 27.959447793164905 34.14775965195958 20.021242882360298 17.871549672515215 8 26.77213205644383 34.74060672328347 19.041645624553098 19.44561559571961 9 26.854756135417006 16.79782273969091 20.363746716272 35.98367440862009 10-11 25.28939662801095 27.150909405400064 26.202296177207803 21.357397789381185 12-13 26.467021409674896 24.727765242598196 27.259961606829847 21.545251740897065 14-15 23.21952831689346 25.834078494419398 26.49121018793503 24.45518300075211 16-17 21.584443744324393 29.180932899670736 27.097676785460568 22.136946570544303 18-19 21.299115876577158 26.850900551848383 30.639322507946844 21.21066106362762 20-21 22.602853041986762 25.79131423519112 30.594884060782785 21.01094866203933 22-23 22.07711529224986 25.90954053290214 30.526450515898574 21.486893658949423 24-25 21.740287037594726 26.21739244584262 30.757431147887836 21.28488936867482 26-27 21.673514387549663 26.350363286666646 30.712177772149367 21.263944553634325 28-29 21.25423141056287 26.49959722918393 30.92360793060757 21.32256342964563 30-31 21.475068635746652 26.376378144594444 30.722037822876626 21.426515396782282 32-33 21.370589559468524 26.558961545634514 30.40974589638238 21.660702998514587 34-35 21.85334430449054 26.457562124949945 30.393316758185218 21.295776812374292 36-37 21.953085944044552 26.193484779415588 30.358094227349223 21.495335049190636 38-39 21.74554203363464 26.464440139331074 30.380899468087673 21.40911835894661 40-41 21.70855708767756 26.402010817517922 30.538508295781902 21.350923799022617 42-43 21.586393266356595 26.611349148129797 30.00091598946574 21.80134159604787 44-45 21.62244052843346 26.73863969697356 29.875223043736867 21.763696730856115 46-47 21.924658443533254 26.995487653608862 29.204471650054792 21.875382252803092 48-49 22.01039569980243 27.077439422188004 28.926890766695422 21.985274111314148 50-51 21.921826288413463 27.333455589864254 28.764282129683004 21.980435992039283 52-53 22.24359183654544 27.402424956429495 28.539807920761856 21.81417528626321 54-55 21.771115992425226 27.26330912015225 28.719731672390115 22.245843215032405 56-57 22.018983967081738 27.59386106143129 28.26902379784012 22.118131173646848 58-59 22.14060655815885 27.109609060239737 28.450603934246573 22.29918044735484 60-61 22.13367034530212 27.214932781016742 28.846552596918933 21.804844276762196 62-63 22.090849192595122 27.145973805745992 28.80530101307126 21.957875988587624 64-65 21.832685213267634 27.132256455926452 28.774001887698358 22.261056443107556 66-67 21.469407362332113 27.336444476409277 29.039941672367668 22.15420648889094 68-69 21.54264434131821 26.924140588585033 29.55365196939428 21.979563100702475 70-71 21.130405207185245 26.96844388706233 29.787074059485864 22.11407684626656 72-73 21.868248825731236 27.234691918026517 28.573273131894428 22.323786124347823 74-75 21.58056668076705 27.651574615906316 28.378466161785276 22.389392541541355 76-77 21.813939811563845 27.521509867904708 28.26430334054335 22.400246979988093 78-79 21.702015272080267 27.586932548312166 28.26045321998715 22.45059895962042 80-81 21.617692794623636 27.917124436318332 28.107413754460175 22.357769014597856 82-83 21.85684876395922 27.82456591471033 27.828253110757966 22.490332210572483 84-85 21.678859509722614 27.641345089118175 27.736748873764306 22.943046527394902 86-87 21.698196800777442 27.565645335749263 27.965369586633077 22.77078827684022 88-89 21.797612339188298 27.522423050438327 27.713733982053068 22.96623062832031 90-91 21.58217756755489 27.66285597460491 27.897860556740582 22.857105901099615 92-93 21.695323997041697 27.65935759471861 27.815593085792838 22.829725322446848 94-95 21.206895651068585 27.388394917124376 28.250593143392017 23.15411628841502 96-97 21.133015505705803 27.367493353868298 28.174645249418585 23.32484589100731 98-99 21.372956631656663 27.3646618116426 27.641027096502146 23.62135446019859 100-101 21.31221758768115 27.174790842335618 27.957190612206677 23.555800957776555 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4394.0 1 2944.5 2 1607.5 3 1805.5 4 1694.0 5 1668.5 6 2599.0 7 4929.0 8 7329.0 9 8462.5 10 7851.5 11 6392.0 12 5670.0 13 5475.5 14 5543.5 15 5560.0 16 5538.0 17 5666.0 18 5886.5 19 6179.0 20 6678.5 21 7466.5 22 8503.0 23 9800.0 24 11160.0 25 13003.5 26 15831.0 27 19080.0 28 22023.5 29 25459.0 30 29349.5 31 34260.0 32 40568.5 33 46346.5 34 51224.5 35 55674.5 36 61975.5 37 68187.5 38 70905.0 39 71636.0 40 72290.0 41 74432.0 42 77208.0 43 80113.5 44 83458.5 45 86293.0 46 88039.5 47 91807.0 48 100786.0 49 101203.0 50 92755.0 51 87745.0 52 85465.5 53 85387.5 54 83977.5 55 78963.5 56 72314.5 57 65493.5 58 59637.5 59 53393.0 60 45669.0 61 37736.0 62 30381.0 63 23631.0 64 17752.5 65 13199.0 66 9566.5 67 7288.5 68 5322.0 69 3561.0 70 2633.0 71 2025.5 72 1674.0 73 1434.5 74 1102.0 75 793.0 76 529.5 77 315.0 78 190.5 79 123.5 80 80.5 81 55.0 82 35.0 83 22.0 84 13.0 85 8.0 86 5.0 87 2.0 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023513114062927117 2 0.018339456781429196 3 0.0 4 0.0 5 1.544375307909827E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 4.6331259237294807E-4 18-19 8.107970366526591E-4 20-21 5.791407404661851E-5 22-23 0.0 24-25 0.0 26-27 3.6678913562858395E-4 28-29 0.018609722460313418 30-31 0.003996071109216678 32-33 0.0075288296260604065 34-35 0.008609892341597284 36-37 0.10078979353246509 38-39 0.10353105970400502 40-41 0.11723739056170475 42-43 0.10339592686456292 44-45 0.09833809773115824 46-47 0.09990177773041695 48-49 0.10146545772967565 50-51 0.09994038711311469 52-53 0.07712224193874699 54-55 0.0662343960179827 56-57 0.03723874961197571 58-59 0.015424448387749397 60-61 0.008687111106992777 62-63 0.0018725550608406651 64-65 0.004536602466985117 66-67 0.41777282548095707 68-69 0.8075731531973974 70-71 0.8989422573516126 72-73 0.27644318011585906 74-75 0.14771949820157496 76-77 0.013918682462537316 78-79 0.008494064193504049 80-81 0.0015636799992586998 82-83 0.0 84-85 0.0 86-87 3.2817975293083823E-4 88-89 7.528829626060406E-4 90-91 0.003648586664936966 92-93 0.0016795081473519368 94-95 0.2208263643397564 96-97 0.22524713865864826 98-99 0.013725635549048588 100-101 0.007393696786618297 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2590044.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.98708740424607 #Duplication Level Percentage of deduplicated Percentage of total 1 69.04364926666977 29.67985385734443 2 14.85755944237014 12.773664127258932 3 6.0894115844105885 7.852982040584594 4 3.066489019539172 5.2727772602836485 5 1.8430749193874658 3.961421132614138 6 1.131596278654362 2.9186416882100783 7 0.780455276971455 2.34846494443939 8 0.5405772003144682 1.859027148692854 9 0.4062662368428959 1.5717782009303727 >10 1.9868238158833766 15.338797652445258 >50 0.15648559652917657 4.634280909757234 >100 0.09068546967035414 7.131085687067689 >500 0.005103289399824086 1.4806674228710852 >1k 0.0014580826856634146 1.177106509046119 >5k 9.113016785396341E-5 0.22278370330621936 >10k+ 2.7339050356189025E-4 1.776667715148051 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17905 0.6913009972031363 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16196 0.625317562172689 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11252 0.43443277411503434 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5687 0.21957155940207965 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4397 0.16976545572198773 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3402 0.13134911993773077 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.1582814809323703E-4 0.0 10-11 0.0 0.0 0.0 1.1582814809323703E-4 0.0 12-13 0.0 0.0 0.0 1.544375307909827E-4 0.0 14-15 3.8609382697745675E-5 0.0 0.0 2.509609875353469E-4 0.0 16-17 3.8609382697745675E-5 0.0 0.0 4.440079010240753E-4 0.0 18-19 3.8609382697745675E-5 0.0 0.0 5.598360491173123E-4 0.0 20-21 3.8609382697745675E-5 0.0 0.0 8.301017280015321E-4 0.0 22-23 3.8609382697745675E-5 0.0 0.0 0.0011582814809323703 0.0 24-25 3.8609382697745675E-5 0.0 0.0 0.001911164443538411 0.0 26-27 3.8609382697745675E-5 0.0 0.0 0.002606133332097833 0.0 28-29 3.8609382697745675E-5 0.0 0.0 0.004729649380473845 0.0 30-31 3.8609382697745675E-5 0.0 0.0 0.00986469727927402 0.0 32-33 3.8609382697745675E-5 0.0 0.0 0.019536347645059314 0.0 34-35 3.8609382697745675E-5 0.0 0.0 0.03372529578648085 0.0 36-37 7.721876539549135E-5 0.0 0.0 0.058473910095735826 0.0 38-39 7.721876539549135E-5 0.0 0.0 0.09482464390566339 0.0 40-41 7.721876539549135E-5 0.0 0.0 0.13565406610852943 0.0 42-43 7.721876539549135E-5 0.0 0.0 0.17553755843530072 0.0 44-45 7.721876539549135E-5 0.0 0.0 0.22464869322683323 0.0 46-47 7.721876539549135E-5 0.0 0.0 0.2853233381363406 0.0 48-49 7.721876539549135E-5 0.0 0.0 0.35316002353627973 0.0 50-51 7.721876539549135E-5 0.0 0.0 0.42547539732915735 1.9304691348872837E-5 52-53 7.721876539549135E-5 0.0 0.0 0.4947792392716108 3.8609382697745675E-5 54-55 9.65234567443642E-5 0.0 0.0 0.5720173093584511 3.8609382697745675E-5 56-57 1.544375307909827E-4 0.0 0.0 0.6589463345024255 3.8609382697745675E-5 58-59 1.544375307909827E-4 0.0 0.0 0.7468598989051923 7.721876539549135E-5 60-61 1.544375307909827E-4 0.0 0.0 0.8378236045410812 1.1582814809323703E-4 62-63 1.9304691348872837E-4 0.0 0.0 0.9267989269680361 1.1582814809323703E-4 64-65 1.9304691348872837E-4 3.8609382697745675E-5 0.0 1.0220482740833745 1.1582814809323703E-4 66-67 1.9304691348872837E-4 3.8609382697745675E-5 0.0 1.1184945120623433 1.1582814809323703E-4 68-69 1.9304691348872837E-4 3.8609382697745675E-5 0.0 1.2392646611408917 1.1582814809323703E-4 70-71 1.9304691348872837E-4 3.8609382697745675E-5 0.0 1.403354537606311 1.1582814809323703E-4 72-73 1.9304691348872837E-4 3.8609382697745675E-5 0.0 1.57686510344998 1.1582814809323703E-4 74-75 1.9304691348872837E-4 3.8609382697745675E-5 0.0 1.7622866638559036 1.1582814809323703E-4 76-77 1.9304691348872837E-4 3.8609382697745675E-5 0.0 1.9736344247433635 1.1582814809323703E-4 78-79 1.9304691348872837E-4 3.8609382697745675E-5 0.0 2.2132056443828754 1.1582814809323703E-4 80-81 1.9304691348872837E-4 3.8609382697745675E-5 0.0 2.482718440304489 1.1582814809323703E-4 82-83 1.9304691348872837E-4 3.8609382697745675E-5 0.0 2.7611886130119796 1.1582814809323703E-4 84-85 2.1235160483760123E-4 5.7914074046618515E-5 0.0 3.0406240202869137 1.1582814809323703E-4 86-87 2.509609875353469E-4 7.721876539549135E-5 0.0 3.3277426947186997 1.1582814809323703E-4 88-89 2.7026567888421973E-4 7.721876539549135E-5 0.0 3.640884093088766 1.1582814809323703E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 14145 0.0 33.61607 1 GTATCAA 20640 0.0 29.606846 1 GTACATG 17140 0.0 23.703856 1 ATCAACG 25580 0.0 23.541945 3 TATCAAC 25975 0.0 23.33753 2 TCAACGC 25970 0.0 23.206654 4 TACATGG 17370 0.0 23.05668 2 CAACGCA 26135 0.0 23.042013 5 AACGCAG 26730 0.0 22.493654 6 GAGTACT 14245 0.0 22.351803 12-13 ACATGGG 17470 0.0 21.859463 3 GTACTTT 15080 0.0 21.239834 14-15 AGTACTT 14575 0.0 20.821707 12-13 GTCGGGA 625 0.0 20.474989 2 CGCGGAA 465 0.0 20.378601 82-83 ATTCGCG 455 0.0 20.306017 80-81 ACGCAGA 30075 0.0 19.928852 7 TCGCGGA 465 0.0 19.869137 82-83 CGCAGAG 30245 0.0 19.754173 8 CATGGGA 11205 0.0 19.366493 4 >>END_MODULE