FastQCFastQC Report
Fri 27 May 2016
SRR939516_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939516_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2568745
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127500.4963513310974815No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95510.37181580888721927No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69320.26985940605237185No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA38920.1515136769122665No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA191150.031.5103361
GGTATCA130750.029.4271261
GTACATG146500.026.4009631
TACATGG151000.025.500122
TATCAAC239300.025.0459672
ATCAACG244500.024.8028353
TCAACGC248950.024.3843214
CAACGCA247850.024.0032655
CAACGCG3650.023.9498525
ACATGGG155400.023.7549423
AACGCAG250150.023.7001196
GAGTACT122600.023.28112212-13
AGTACTT127100.021.8514412-13
GTACTTT134950.021.57152414-15
ACGCAGA272750.021.4165867
AACGCGG3650.021.2670356
CGCAGAG276850.021.0464258
CATGGGA100500.020.5454794
GCAGAGT270550.020.3350569
CATGGGG61100.019.5116144
Produced by FastQC (version 0.11.5)