FastQCFastQC Report
Fri 27 May 2016
SRR939516_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939516_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2568745
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176540.6872616783682304No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153790.5986970290939739No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109810.4274850170024662No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA56850.22131429939522998No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40020.1557959236903624No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33060.1287009804398646No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA136900.033.8007161
GTATCAA199350.029.2590641
GTACATG161850.023.8691231
ATCAACG250650.023.242683
TACATGG164200.023.0027662
TATCAAC254000.022.9032382
TCAACGC254000.022.8640944
CAACGCA255400.022.738295
GAGTACT139850.022.6080812-13
AACGCAG260450.022.3702136
ACATGGG170100.021.3080923
GTACTTT148150.021.19749614-15
AGTACTT143250.021.14494512-13
ACGCAGA292700.019.7758677
CATGGGA105800.019.73214
CGCAGAG294600.019.6000488
AGAGTAC252900.019.01605610-11
ACTTTTT172950.018.95273816-17
GCAGAGT296400.018.889239
TACTTTT163250.018.59814-15
Produced by FastQC (version 0.11.5)