##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939516_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2568745 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.90694366315068 31.0 31.0 31.0 30.0 34.0 2 31.14210558074079 31.0 31.0 34.0 28.0 34.0 3 31.13484250090998 31.0 31.0 34.0 28.0 34.0 4 35.057822010359146 35.0 35.0 37.0 32.0 37.0 5 34.63580191883585 35.0 35.0 37.0 32.0 37.0 6 34.707081473637906 35.0 35.0 37.0 31.0 37.0 7 34.540595115513604 35.0 35.0 37.0 30.0 37.0 8 34.44271151865989 35.0 35.0 37.0 30.0 37.0 9 35.92852891197842 37.0 35.0 39.0 30.0 39.0 10-11 35.73032375732118 37.0 35.0 39.0 30.0 39.0 12-13 35.70676049977713 37.0 35.0 39.0 30.0 39.0 14-15 36.50243114828447 38.0 35.0 40.0 30.0 41.0 16-17 36.429337867324314 38.0 35.0 40.0 30.0 41.0 18-19 36.46639214869518 38.0 35.0 40.0 30.0 41.0 20-21 36.35317557795733 38.0 34.5 40.0 29.5 41.0 22-23 36.19026801025403 38.0 34.0 40.0 29.0 41.0 24-25 36.02130904391055 38.0 34.0 40.0 29.0 41.0 26-27 35.55112944258772 38.0 34.0 40.0 27.0 41.0 28-29 35.32909922939023 38.0 34.0 40.0 27.0 41.0 30-31 35.00758113397788 37.0 33.0 40.0 27.0 41.0 32-33 34.72126057666292 37.0 33.0 40.0 25.5 41.0 34-35 34.936223720143495 37.0 33.0 40.0 26.0 41.0 36-37 34.75201450513772 37.0 33.0 40.0 25.0 41.0 38-39 34.5427235868099 37.0 33.0 40.0 25.0 41.0 40-41 34.363846742280764 37.0 33.0 40.0 24.5 41.0 42-43 34.16598767102223 37.0 33.0 40.0 24.0 41.0 44-45 33.73520707582885 37.0 32.5 40.0 23.5 41.0 46-47 33.266563633213885 37.0 32.0 40.0 21.5 41.0 48-49 32.82596832305269 36.5 31.0 39.5 19.0 41.0 50-51 32.55372312160219 36.0 31.0 39.0 18.0 40.0 52-53 32.42948190653412 36.0 31.0 39.0 18.0 40.0 54-55 32.08420123445496 36.0 30.5 39.0 15.5 40.0 56-57 31.58547247780531 35.0 29.5 39.0 12.5 40.0 58-59 31.124334451259273 35.0 29.0 38.5 9.0 40.0 60-61 30.751334017195166 35.0 28.0 38.0 8.0 40.0 62-63 30.59085389947231 34.0 28.0 38.0 8.0 40.0 64-65 30.12886993454002 34.0 28.0 38.0 7.0 40.0 66-67 29.26861210435446 33.5 27.0 37.0 2.0 39.5 68-69 25.873019509527026 31.0 16.5 35.5 2.0 39.0 70-71 24.585630920936097 29.5 12.0 35.0 2.0 38.0 72-73 25.583411549219562 30.5 18.5 35.0 2.0 37.5 74-75 26.30866571029822 31.0 21.5 35.0 2.0 37.0 76-77 25.972288218565875 30.5 22.0 34.0 2.0 36.0 78-79 27.06996529433634 32.0 25.0 35.0 2.0 36.5 80-81 27.09078110127708 32.0 25.0 35.0 2.0 36.0 82-83 26.771639652826572 32.0 25.0 35.0 2.0 36.0 84-85 26.414859396319994 32.0 24.5 34.5 2.0 35.5 86-87 25.97095507728482 32.0 23.5 34.0 2.0 35.0 88-89 25.48378001709006 31.0 21.5 34.0 2.0 35.0 90-91 24.89601984626734 31.0 19.5 34.0 2.0 35.0 92-93 24.34391191029082 31.0 17.5 34.0 2.0 35.0 94-95 23.50974230606775 30.0 7.0 34.0 2.0 35.0 96-97 22.842357454710374 30.0 2.0 34.0 2.0 35.0 98-99 22.004797284276954 29.5 2.0 34.0 2.0 35.0 100-101 20.61550757276413 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.2222708905601145 1101 2 0.15861557671180293 1101 3 0.3644182662086841 1101 4 0.24392182893679148 1101 5 0.47167999928992543 1101 6 0.39971212650403487 1101 7 0.4538894642095528 1101 8 0.6172219935069094 1101 9 0.6164890592043903 1101 10-11 0.6254721588794752 1101 12-13 0.5402107292194529 1101 14-15 0.6781729629703577 1101 16-17 0.6384928763408837 1101 18-19 0.5309168980975585 1101 20-21 0.5099897222376555 1101 22-23 0.6271398461298006 1101 24-25 0.6589568068885896 1101 26-27 0.6299889597074397 1101 28-29 0.5782238978072414 1101 30-31 0.2707942141039652 1101 32-33 0.6724380487512249 1101 34-35 0.6039763957065389 1101 36-37 0.9079604511033494 1101 38-39 0.8299945080024713 1101 40-41 0.7643631661710373 1101 42-43 0.5965915720507198 1101 44-45 0.8138631440957056 1101 46-47 0.5560675651291476 1101 48-49 0.6335640569422125 1101 50-51 0.4360111574465151 1101 52-53 0.22384412273526522 1101 54-55 0.18754495500712665 1101 56-57 0.3662335207975502 1101 58-59 0.3304218003464783 1101 60-61 0.30229732802043685 1101 62-63 0.09524608038133664 1101 64-65 0.026965990710422716 1101 66-67 -0.1668884421605945 1101 68-69 -7.183018517740361 1101 70-71 -7.818439776781172 1101 72-73 -4.9962307375834065 1101 74-75 -3.1169984466130067 1101 76-77 -0.4783380907200332 1101 78-79 -0.1427463113492955 1101 80-81 -0.27513160071427833 1101 82-83 -0.5078666549998587 1101 84-85 -0.5631924456738133 1101 86-87 -0.6158664190209144 1101 88-89 -0.6406794440598809 1101 90-91 -0.5602830545883535 1101 92-93 -0.5796024541144149 1101 94-95 -0.6963608193860864 1101 96-97 -0.9882499078788243 1101 98-99 -1.247406610461212 1101 100-101 -1.0799357557060993 1102 1 0.29926381391854306 1102 2 0.3263342115673211 1102 3 0.3971527438429092 1102 4 0.40386024151241173 1102 5 0.40190876761158734 1102 6 0.40919374397057595 1102 7 0.46523894628797535 1102 8 0.5934994114847072 1102 9 0.7387026691633949 1102 10-11 0.6458417410449755 1102 12-13 0.5244274486118812 1102 14-15 0.7293457143930553 1102 16-17 0.5953094047381313 1102 18-19 0.5258400190347814 1102 20-21 0.6607010439622982 1102 22-23 0.6896454201624707 1102 24-25 0.5452717174385171 1102 26-27 0.5605271181572746 1102 28-29 0.6682352563079803 1102 30-31 0.4826140944363644 1102 32-33 0.8275550483233403 1102 34-35 0.6290924858971394 1102 36-37 0.7539895728562556 1102 38-39 0.8758448097768365 1102 40-41 0.7027525688451703 1102 42-43 0.4042957119160988 1102 44-45 0.44796153115854054 1102 46-47 0.6905684725065129 1102 48-49 0.7394576507878909 1102 50-51 0.7211947648301944 1102 52-53 0.49927386186552525 1102 54-55 0.25042774700376214 1102 56-57 0.4745883945313061 1102 58-59 0.27498792014834805 1102 60-61 0.3856393251124217 1102 62-63 0.19872793433717106 1102 64-65 0.1644147471875712 1102 66-67 0.19519808987418585 1102 68-69 -6.618182622272638 1102 70-71 -7.477297703832242 1102 72-73 -1.5676185013666704 1102 74-75 -0.13394024920256697 1102 76-77 0.5052974261644003 1102 78-79 0.1804296522193276 1102 80-81 -0.03158990043640486 1102 82-83 -0.2319034371020301 1102 84-85 -0.24680584179037623 1102 86-87 -0.2101658996463307 1102 88-89 -0.2860664998783662 1102 90-91 -0.2879206617976706 1102 92-93 -0.3662426376541603 1102 94-95 -0.2959519985357453 1102 96-97 -0.49730658364097735 1102 98-99 -0.31772679041353413 1102 100-101 -0.4469913812954438 1103 1 0.3631605400879039 1103 2 0.2532785337738943 1103 3 0.2926709908013372 1103 4 0.28565686701950455 1103 5 0.40777027339805016 1103 6 0.33105000777815263 1103 7 0.39315776755217513 1103 8 0.5514453803056938 1103 9 0.5694975339490185 1103 10-11 0.5572597519010216 1103 12-13 0.5138150583717049 1103 14-15 0.6423653790076074 1103 16-17 0.6324467241451117 1103 18-19 0.6018769917051472 1103 20-21 0.5866850684784524 1103 22-23 0.5507246074821026 1103 24-25 0.7197495908402161 1103 26-27 0.8011178131734269 1103 28-29 0.7745117768961194 1103 30-31 0.7159571930184114 1103 32-33 0.7018257554050749 1103 34-35 0.7545828981758547 1103 36-37 0.94264735637136 1103 38-39 0.9053014840721403 1103 40-41 0.8756478270024672 1103 42-43 0.7150030838768231 1103 44-45 0.8057626212281974 1103 46-47 0.6138695548863495 1103 48-49 0.6854122704120229 1103 50-51 0.39849288092270996 1103 52-53 0.5149166368300442 1103 54-55 0.4302460166347686 1103 56-57 0.6391926588607895 1103 58-59 0.6400963888267093 1103 60-61 0.5053149906914314 1103 62-63 0.1875450088207984 1103 64-65 0.22856647781951267 1103 66-67 0.3206432934337293 1103 68-69 -6.7960210724805385 1103 70-71 -7.714776625034375 1103 72-73 -1.0348316994847977 1103 74-75 0.3755161249415693 1103 76-77 0.6221356089648999 1103 78-79 0.35956979106280684 1103 80-81 0.0679944894207658 1103 82-83 -0.008025084649148084 1103 84-85 -0.10265036795090765 1103 86-87 -0.07488578418983494 1103 88-89 0.051494727238829086 1103 90-91 0.05888683364673142 1103 92-93 -0.005185713613244047 1103 94-95 0.26726244186092885 1103 96-97 0.30074141673210164 1103 98-99 0.18302067281298662 1103 100-101 0.1588259204100595 1104 1 0.2654101757113416 1104 2 0.3037140674720362 1104 3 0.30384565542517095 1104 4 0.29803622020875764 1104 5 0.4395917978543622 1104 6 0.25125215393033784 1104 7 0.45456669353844603 1104 8 0.5984256570895212 1104 9 0.4584479772636101 1104 10-11 0.4072127597197195 1104 12-13 0.38486852514041203 1104 14-15 0.6004057424563598 1104 16-17 0.5468315475237375 1104 18-19 0.4761826635104782 1104 20-21 0.6119043080519262 1104 22-23 0.6277474190013592 1104 24-25 0.4730046799621306 1104 26-27 0.6563960697229945 1104 28-29 0.5450013620345615 1104 30-31 0.48936712929363324 1104 32-33 0.6689985442689235 1104 34-35 0.29387316037480105 1104 36-37 0.5159295092588039 1104 38-39 0.5672387622123409 1104 40-41 0.6431154430200081 1104 42-43 0.5427503530775866 1104 44-45 0.6248738345027434 1104 46-47 0.5716694960755575 1104 48-49 0.6328546022060308 1104 50-51 0.4504860047724506 1104 52-53 0.4216175206119601 1104 54-55 0.26611204731358384 1104 56-57 0.3676376189769641 1104 58-59 0.47503945084656607 1104 60-61 0.5433495098788192 1104 62-63 0.360025643722107 1104 64-65 0.3011150912249363 1104 66-67 0.2509641608094988 1104 68-69 -6.557816613169212 1104 70-71 -4.208711025471086 1104 72-73 0.29710307747845377 1104 74-75 0.9495611490721565 1104 76-77 1.1419327068141811 1104 78-79 0.7765056799329884 1104 80-81 0.4346745783340502 1104 82-83 0.4240998087624206 1104 84-85 0.4435188672289456 1104 86-87 0.36000654247418495 1104 88-89 0.35543491548245854 1104 90-91 0.15678146365172552 1104 92-93 0.13893104692323988 1104 94-95 0.35317124291108115 1104 96-97 0.35734496570337626 1104 98-99 0.5664727609152891 1104 100-101 0.8558959597465297 1105 1 0.11627318608640635 1105 2 0.10819548749840635 1105 3 0.09161215323402416 1105 4 0.1694703253868255 1105 5 0.3585112445240455 1105 6 0.13685757436872592 1105 7 0.28626270651203356 1105 8 0.3355152835373687 1105 9 0.32568321489717533 1105 10-11 0.28588959105082523 1105 12-13 0.33864742050079855 1105 14-15 0.4057155250305655 1105 16-17 0.3352611625191315 1105 18-19 0.42794120855069906 1105 20-21 0.44347181041589323 1105 22-23 0.49928574835855244 1105 24-25 0.4366112807942599 1105 26-27 0.4841010532985379 1105 28-29 0.43222585749922615 1105 30-31 0.3788661893233396 1105 32-33 0.5272373679953191 1105 34-35 0.6156733072161771 1105 36-37 0.7252396696761707 1105 38-39 0.7684797866066617 1105 40-41 0.6149420852586758 1105 42-43 0.4919544904113806 1105 44-45 0.5962585613402069 1105 46-47 0.7242217802448536 1105 48-49 0.809399988052725 1105 50-51 0.6811783535460947 1105 52-53 0.5645756883948252 1105 54-55 0.5750234221012818 1105 56-57 0.697363929211587 1105 58-59 0.8114992654691022 1105 60-61 0.9693410674762291 1105 62-63 0.6546255375940682 1105 64-65 0.5449780688926502 1105 66-67 0.5030580416541675 1105 68-69 -2.835644346954787 1105 70-71 -2.820127344194976 1105 72-73 1.0241921864666814 1105 74-75 1.4183505840111295 1105 76-77 1.3023981568649248 1105 78-79 0.8693657012464655 1105 80-81 0.6041297909831052 1105 82-83 0.6061515630237722 1105 84-85 0.6361563320868449 1105 86-87 0.5933707037087466 1105 88-89 0.5705124319105828 1105 90-91 0.6065130070629259 1105 92-93 0.6180266544232822 1105 94-95 0.5172668430885778 1105 96-97 0.3160804286271528 1105 98-99 0.28182554389613657 1105 100-101 0.45408154371191856 1106 1 0.11035795329685172 1106 2 0.22923518552232025 1106 3 0.16930694112990707 1106 4 0.12881197599093497 1106 5 0.180617437619496 1106 6 0.1414383416105096 1106 7 0.199563581725549 1106 8 0.2753996918867685 1106 9 0.2818623045810966 1106 10-11 0.343055891412817 1106 12-13 0.16750894754074608 1106 14-15 0.310484390700573 1106 16-17 0.2441328363943569 1106 18-19 0.178101344416433 1106 20-21 0.31686726862972847 1106 22-23 0.19459983992296515 1106 24-25 0.3034083578005138 1106 26-27 0.4475981561615825 1106 28-29 0.352696118261143 1106 30-31 0.30064618517057795 1106 32-33 0.454610121196076 1106 34-35 0.4452774826976551 1106 36-37 0.43948946007937906 1106 38-39 0.5094012583287224 1106 40-41 0.41643996774344316 1106 42-43 0.37820562654351164 1106 44-45 0.48460370653005214 1106 46-47 0.5999132537690528 1106 48-49 0.6261516687447468 1106 50-51 0.5744284554062133 1106 52-53 0.2730432028511274 1106 54-55 0.3031198229130325 1106 56-57 0.48106983553160987 1106 58-59 0.7043007910203194 1106 60-61 0.7401002094222235 1106 62-63 0.09993203862050493 1106 64-65 0.4599158068927416 1106 66-67 0.6290908445313228 1106 68-69 -2.6449817860345988 1106 70-71 -2.7942933154704157 1106 72-73 0.9472363741713785 1106 74-75 1.3348429131603616 1106 76-77 1.2970835559998974 1106 78-79 1.0329726465397435 1106 80-81 0.6899102158181698 1106 82-83 0.5719030556663398 1106 84-85 0.5299955576364965 1106 86-87 0.631613548035137 1106 88-89 0.7841542447073486 1106 90-91 0.7723180252410415 1106 92-93 0.6689388412034063 1106 94-95 0.8333603506810761 1106 96-97 0.9731876697065545 1106 98-99 0.7689693762657193 1106 100-101 0.8676309478302038 1107 1 0.056526588778901043 1107 2 0.10011044227985266 1107 3 0.03956408737735728 1107 4 -0.14274721736078533 1107 5 0.3221261285038892 1107 6 0.21693068184799813 1107 7 0.12096534486212107 1107 8 0.13530546903350427 1107 9 0.12889082205697378 1107 10-11 0.04855845591931285 1107 12-13 0.14840544561352687 1107 14-15 0.010001231334953786 1107 16-17 -0.022624556381245498 1107 18-19 0.18428935435120763 1107 20-21 0.1925885680478885 1107 22-23 0.4196435961117473 1107 24-25 0.0854249222415362 1107 26-27 0.44593370756629014 1107 28-29 0.4503961947769213 1107 30-31 0.4489861863571889 1107 32-33 0.33033691890796746 1107 34-35 0.2530722219302035 1107 36-37 0.44589471655049095 1107 38-39 0.4951114486361803 1107 40-41 0.2632714417157942 1107 42-43 0.14143145246395505 1107 44-45 0.3683060989783087 1107 46-47 0.5365534740690663 1107 48-49 0.44423742371417774 1107 50-51 0.4969851159625307 1107 52-53 0.24145640039279925 1107 54-55 0.3119036668290107 1107 56-57 0.7299261904339396 1107 58-59 0.7601381376818566 1107 60-61 0.5867825901271502 1107 62-63 0.3137008402277459 1107 64-65 0.051088897271394984 1107 66-67 0.2349381136950477 1107 68-69 -2.824885957168096 1107 70-71 -2.5517335298373816 1107 72-73 0.9309882768367466 1107 74-75 1.069001689562402 1107 76-77 0.5767683324264858 1107 78-79 0.5848284613306127 1107 80-81 0.49260253846111723 1107 82-83 0.33796421101385477 1107 84-85 0.3051439859939862 1107 86-87 0.6039807859787878 1107 88-89 0.5303836938754962 1107 90-91 0.4458420018934959 1107 92-93 0.5446243377786075 1107 94-95 0.6813343189454386 1107 96-97 0.5110886871210596 1107 98-99 0.5827732390855935 1107 100-101 0.8205823040418956 1108 1 -0.04325491158526518 1108 2 -0.06704177913311327 1108 3 -0.05584742027012979 1108 4 -0.06954983935641934 1108 5 -0.13344561220987572 1108 6 -0.1268420785307356 1108 7 -0.027978573564681142 1108 8 0.17645623378223263 1108 9 0.11213918330970785 1108 10-11 0.2290027385121718 1108 12-13 0.09778636331200374 1108 14-15 0.08064944692126375 1108 16-17 0.19478258127352888 1108 18-19 0.1828326901123205 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0.4892236334945679 2312 36-37 0.6966047960645128 2312 38-39 0.6379653764019366 2312 40-41 0.2981278043436788 2312 42-43 0.10074161030468787 2312 44-45 0.6247420030936581 2312 46-47 0.6608532739992441 2312 48-49 0.8021358399905409 2312 50-51 0.745806671049074 2312 52-53 0.7320254740996575 2312 54-55 0.613195703199537 2312 56-57 0.6548813120618568 2312 58-59 0.7100170148099245 2312 60-61 0.5791151667748053 2312 62-63 0.7600114215998133 2312 64-65 0.9448879732997 2312 66-67 1.2906584447937703 2312 68-69 3.8702113575723907 2312 70-71 4.780060736717996 2312 72-73 2.8402424363928738 2312 74-75 2.20041468931608 2312 76-77 2.143929680142165 2312 78-79 1.4325031148412286 2312 80-81 1.247634764056233 2312 82-83 1.2249273318904024 2312 84-85 1.2592292026240592 2312 86-87 1.1812271412170183 2312 88-89 1.4338671947606585 2312 90-91 1.4968516545092 2312 92-93 1.7801142253035707 2312 94-95 1.9134589044961068 2312 96-97 2.05057864198573 2312 98-99 2.275621673348745 2312 100-101 2.1488313121102536 2313 1 -0.39922764230931307 2313 2 -0.18736534108452219 2313 3 -0.2004254194261108 2313 4 -0.14324157075013488 2313 5 0.05054460500254976 2313 6 -0.03546605222605592 2313 7 -2.6435089932436604 2313 8 -3.0832190962890884 2313 9 -0.9916017064286322 2313 10-11 -0.038651983181857474 2313 12-13 -0.010305618354230717 2313 14-15 -0.5154738546554896 2313 16-17 -0.3415327588347594 2313 18-19 0.12756515433524385 2313 20-21 -2.5755246607119417 2313 22-23 -0.6270314944409492 2313 24-25 0.024562101233641442 2313 26-27 -1.4237588776216867 2313 28-29 -1.7744506843932655 2313 30-31 -0.937694704782416 2313 32-33 -0.8992668488665103 2313 34-35 -1.4589433842555621 2313 36-37 -2.2042884255752995 2313 38-39 -2.02548275305805 2313 40-41 -1.158904479005173 2313 42-43 -0.07843995699719386 2313 44-45 -0.14200957678191628 2313 46-47 -0.3292216782370758 2313 48-49 -2.497046053900604 2313 50-51 -0.7901753012234991 2313 52-53 -0.5570484820394057 2313 54-55 -0.3371121071827332 2313 56-57 -0.4014063477627623 2313 58-59 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-3.4063135035189447 2315 38-39 -1.0006408256889046 2315 40-41 0.06723213848334098 2315 42-43 -1.5641387950459986 2315 44-45 -5.102232901924282 2315 46-47 -3.3478209912322185 2315 48-49 -2.6770571910199124 2315 50-51 -1.12770609827216 2315 52-53 -0.05707632483767355 2315 54-55 -1.0855712025507955 2315 56-57 -1.1747280907219313 2315 58-59 -0.594422054066591 2315 60-61 -1.352788242016011 2315 62-63 -0.24756531228493017 2315 64-65 0.12693486750077554 2315 66-67 0.28051657909611194 2315 68-69 3.0157918308805804 2315 70-71 3.5066303614728405 2315 72-73 -2.4156358879792243 2315 74-75 -1.7890114042540155 2315 76-77 -0.23078097228511396 2315 78-79 -0.11508192887340485 2315 80-81 -0.31746676727640377 2315 82-83 -1.0846733385297753 2315 84-85 -0.602205440211705 2315 86-87 -0.004424317756718921 2315 88-89 0.17004805327876227 2315 90-91 0.17788517199681309 2315 92-93 -1.0995122548848784 2315 94-95 0.004031825013989021 2315 96-97 -0.28507897893813805 2315 98-99 -2.681052422869225 2315 100-101 -4.199270631192761 2316 1 -0.5292680815566193 2316 2 -0.4076164262249229 2316 3 -0.8925927450985718 2316 4 -0.32204641626861985 2316 5 -0.16139457994268014 2316 6 -0.5443989797062017 2316 7 -0.5685102011400431 2316 8 -1.0735372730509596 2316 9 -4.9243999697545355 2316 10-11 -1.380997908975452 2316 12-13 -0.6300712976920124 2316 14-15 -0.3713418152881758 2316 16-17 -0.3850516362888996 2316 18-19 -0.43995254556036656 2316 20-21 -3.6744628355187103 2316 22-23 -1.1748547527948219 2316 24-25 -0.5280524045344848 2316 26-27 -0.6623992020345639 2316 28-29 0.13607790230054917 2316 30-31 0.02205324376007667 2316 32-33 -1.2864814622778695 2316 34-35 -0.5953630894839037 2316 36-37 -0.1881996951923739 2316 38-39 -5.1096443662233355 2316 40-41 -4.988233326829331 2316 42-43 -3.539639740811836 2316 44-45 -2.390905784211995 2316 46-47 -2.5736790069096145 2316 48-49 -2.681498910547461 2316 50-51 -1.0601901443037107 2316 52-53 -0.8560175565211061 2316 54-55 -0.01496984957383063 2316 56-57 -3.8955138359338015 2316 58-59 -0.8421471643857501 2316 60-61 -0.4294654166535423 2316 62-63 -0.1657595355418806 2316 64-65 0.2954509079655452 2316 66-67 0.1612857567486614 2316 68-69 2.8627764405919613 2316 70-71 3.8201175128920433 2316 72-73 2.411755608196401 2316 74-75 1.2669122250639298 2316 76-77 -1.1855917306834378 2316 78-79 0.15715859002700938 2316 80-81 0.2297808203362557 2316 82-83 0.46429169781443136 2316 84-85 0.5704144510839768 2316 86-87 0.571308908195185 2316 88-89 0.24391276478928958 2316 90-91 0.34499946320062236 2316 92-93 0.13233266326221838 2316 94-95 0.3736829540817723 2316 96-97 0.562894281340931 2316 98-99 0.8273228199928262 2316 100-101 0.9432501139936669 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 38.0 4 118.0 5 244.0 6 658.0 7 1457.0 8 3061.0 9 5169.0 10 7967.0 11 11578.0 12 15045.0 13 19072.0 14 23262.0 15 29342.0 16 34666.0 17 38968.0 18 34936.0 19 30129.0 20 29587.0 21 30056.0 22 31399.0 23 35273.0 24 42106.0 25 51366.0 26 63997.0 27 79675.0 28 96778.0 29 114923.0 30 134752.0 31 156934.0 32 179980.0 33 207105.0 34 237399.0 35 263266.0 36 261925.0 37 208208.0 38 83646.0 39 4654.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.01239764351324 20.426363682380472 15.626482051841151 21.93475662226514 2 16.05696517471049 21.633442432482735 38.909031867698005 23.40056052510877 3 19.31708691957235 25.7931250878352 30.62383721767674 24.265950774915705 4 13.005864131387716 17.844571294979445 36.5089520543942 32.64061251923864 5 13.279013681778457 38.55256165948742 33.398215860274185 14.770208798459949 6 31.158250429684536 36.75534161623673 17.05762930925413 15.028778644824614 7 27.864540855553976 34.17657260646736 20.08272522184958 17.87616131612908 8 26.811186007174708 34.714578519860865 19.089282898847493 19.38495257411693 9 26.769687142943344 16.843010886639195 20.37683771647244 36.01046425394502 10-11 25.316361811428212 27.12407962827524 26.244661440057747 21.314897120238804 12-13 26.378601223554693 24.773186906446533 27.335138365232826 21.513073504765945 14-15 23.13882849407006 25.834600164671855 26.558222011137733 24.46834933012035 16-17 21.58032087043741 29.1795191517681 27.109898407054224 22.130261570740267 18-19 21.257861096571997 26.86860472336356 30.710135683174038 21.163398496890405 20-21 22.612481970767824 25.81237141094192 30.632448919608603 20.942697698681652 22-23 22.070383896235548 25.958514307203412 30.530462199186292 21.440639597374744 24-25 21.699214651634758 26.204232620084568 30.818762557573592 21.277790170707085 26-27 21.6541205270313 26.387906964431153 30.704276017148295 21.253696491389256 28-29 21.324244465120625 26.481716503614656 30.88343954747714 21.31059948378758 30-31 21.471261888851753 26.395698592364624 30.71261966710923 21.420419851674392 32-33 21.437062295265765 26.559561712778684 30.423363483811922 21.580012508143625 34-35 21.84788596410976 26.476574407328318 30.403984170765746 21.271555457796172 36-37 21.93670945754025 26.236259079926732 30.36236325317659 21.46466820935643 38-39 21.703611767617037 26.50190262236502 30.42374020680424 21.370745403213707 40-41 21.707877250705675 26.400137141343244 30.534890199264037 21.357095408687048 42-43 21.546395086433144 26.625082599290582 30.062245578600237 21.766276735676033 44-45 21.696495571249237 26.733805078861923 29.8553399427578 21.714359407131038 46-47 21.931040555303607 26.941486004422856 29.23691338342162 21.890560056851918 48-49 22.01705475161213 27.020713014533314 29.010102883287775 21.952129350566775 50-51 21.97243369986717 27.28145057247496 28.82750404346902 21.918611684188853 52-53 22.180053914235426 27.44137591341314 28.56202152868924 21.816548643662195 54-55 21.76884327380856 27.223522570526015 28.720772105039405 22.286862050626013 56-57 22.025430073139933 27.50320528733685 28.347239948248117 22.124124691275096 58-59 22.182905270699646 27.089316864992835 28.510606815774718 22.2171710485328 60-61 22.091357427207647 27.210314499250405 28.8982432342198 21.80008483932215 62-63 22.07618511860675 27.160941768672792 28.825981463223783 21.936891649496676 64-65 21.81491611773942 27.140349077434315 28.80545813323014 22.239276671596127 66-67 21.698728414978287 27.384238974154023 28.71241903332245 22.20461357754524 68-69 21.846964436993403 26.911278051615188 29.23339880989375 22.00835870149766 70-71 21.336192803318095 26.94581780913568 29.60199469043475 22.115994697111475 72-73 21.831099817865155 27.172022493770616 28.698075746530176 22.298801941834054 74-75 21.829434332075678 27.622694335452707 28.14504319734138 22.402828135130235 76-77 21.20353334038046 27.55285051681305 28.79870951787129 22.4449066249352 78-79 21.729086812740338 27.513494498340673 28.27096185642091 22.48645683249807 80-81 21.63131169795337 27.88618047599082 28.11279642366476 22.369711402391047 82-83 21.88052139163644 27.760844239455523 27.825916002339703 22.532718366568336 84-85 21.667503328968674 27.694800385481045 27.70488315568128 22.932813129869 86-87 21.726286817125835 27.53840718575696 27.96019764919312 22.77510834792409 88-89 21.788005710194767 27.512273531876925 27.75935974810987 22.940361009818435 90-91 21.587582016012817 27.62077987502674 27.900890338602746 22.890747770357695 92-93 21.662188246268222 27.619681275582863 27.848890491136462 22.869239987012453 94-95 21.470429360277283 27.366538483041168 28.029377887029195 23.133654269652347 96-97 21.477322801742293 27.282250936757684 27.886842514691267 23.353583746808752 98-99 21.353291536355293 27.33524310675945 27.634908166374167 23.676557190511094 100-101 21.311010745989723 27.143688677776044 27.966087836785547 23.579212739448685 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4407.0 1 2982.5 2 1614.0 3 1757.0 4 1658.0 5 1658.0 6 2585.5 7 4921.5 8 7269.0 9 8206.5 10 7501.0 11 6147.5 12 5559.5 13 5384.5 14 5363.5 15 5381.0 16 5424.0 17 5565.0 18 5818.0 19 6235.5 20 6719.5 21 7437.5 22 8471.5 23 9690.0 24 11143.0 25 12995.5 26 15819.5 27 19116.0 28 21902.5 29 25341.0 30 29480.0 31 34333.0 32 40390.5 33 45894.5 34 50944.0 35 55389.5 36 61263.5 37 67654.5 38 70430.5 39 71354.5 40 71985.0 41 73775.0 42 76398.5 43 79666.5 44 83091.5 45 85373.0 46 87363.5 47 90761.0 48 99430.5 49 100358.5 50 92185.5 51 86893.0 52 84223.5 53 84219.0 54 82590.0 55 77659.0 56 71290.0 57 64595.5 58 59274.5 59 53145.0 60 45360.0 61 37659.5 62 30469.5 63 23783.0 64 17784.5 65 13303.5 66 9739.0 67 7406.5 68 5445.5 69 3598.0 70 2578.5 71 1984.0 72 1611.5 73 1361.0 74 1074.5 75 839.5 76 576.0 77 327.5 78 200.5 79 129.5 80 81.0 81 57.0 82 37.5 83 20.0 84 15.5 85 9.5 86 4.5 87 3.0 88 1.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0200487008247218 2 0.017090057596219167 3 5.450132263031169E-4 4 0.08934323959754666 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.011114376864967135 12-13 0.0 14-15 0.0 16-17 5.255484682208627E-4 18-19 1.3625330657577922E-4 20-21 0.0 22-23 1.557180646580334E-4 24-25 0.0029586432285026347 26-27 0.0036204450032992766 28-29 0.0013625330657577922 30-31 1.7518282274028756E-4 32-33 7.201960490434044E-4 34-35 0.0031143612931606676 36-37 0.08689068007918263 38-39 0.09927026621949629 40-41 0.07980550813724212 42-43 0.08319237604355434 44-45 0.08202449055861909 46-47 0.08085660507368383 48-49 0.0760293450692848 50-51 0.07597095079503804 52-53 0.05915339981197044 54-55 0.04945995028700786 56-57 0.028126575428857284 58-59 0.022773766956237385 60-61 0.01403409057730526 62-63 0.002627742341104314 64-65 0.006793200570706708 66-67 0.1569832739333799 68-69 0.7189697692842225 70-71 0.8792425873335034 72-73 0.3543753856455195 74-75 0.054481857872229436 76-77 0.19667191566309616 78-79 0.014929469449088953 80-81 0.0031922203254896846 82-83 0.0016545044369916048 84-85 2.530418550693043E-4 86-87 0.0020437995986366886 88-89 2.335770969870501E-4 90-91 0.004496359117000714 92-93 0.005099766617550594 94-95 0.07725562482846682 96-97 0.03809253156697142 98-99 0.014695892352101904 100-101 0.013430683076755382 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2568745.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.071616716611565 #Duplication Level Percentage of deduplicated Percentage of total 1 70.03696580047462 30.866423127529497 2 14.513001784143844 12.792229040765745 3 5.827800728328545 7.705217999990562 4 3.0003929119072916 5.28928665651265 5 1.7471511341956014 3.849988756613086 6 1.1017818600534264 2.9134384704953984 7 0.7509607491159985 2.316723801298185 8 0.521894174230844 1.8400576010667407 9 0.36920033249408246 1.4644129990792276 >10 1.889378524375206 15.060171086241256 >50 0.15084531403581095 4.601473818053018 >100 0.08415969712563495 6.838101036723596 >500 0.0047604228404395715 1.4002733214492333 >1k 0.0013472894831428471 1.0948764572271932 >5k 8.98192988761898E-5 0.22503968543102043 >10k+ 2.694578966285694E-4 1.7422861415234712 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17654 0.6872616783682304 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15379 0.5986970290939739 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10981 0.4274850170024662 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5685 0.22131429939522998 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4002 0.1557959236903624 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3306 0.1287009804398646 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 2.335770969870501E-4 0.0 10-11 0.0 0.0 0.0 2.335770969870501E-4 0.0 12-13 0.0 0.0 0.0 3.3090088739832097E-4 0.0 14-15 0.0 0.0 0.0 4.282246778095918E-4 0.0 16-17 0.0 0.0 0.0 5.450132263031169E-4 0.0 18-19 0.0 0.0 0.0 6.618017747966419E-4 0.0 20-21 0.0 0.0 0.0 8.759141137014378E-4 0.0 22-23 0.0 0.0 0.0 0.0013041387915110297 0.0 24-25 0.0 0.0 0.0 0.001654504436991605 0.0 26-27 0.0 0.0 0.0 0.0026472070991865678 0.0 28-29 0.0 0.0 0.0 0.004691006697823257 0.0 30-31 0.0 0.0 0.0 0.00979077331537385 0.0 32-33 0.0 0.0 0.0 0.01995137703431053 0.0 34-35 0.0 0.0 0.0 0.0342774389828496 0.0 36-37 0.0 0.0 0.0 0.05954269497361552 0.0 38-39 0.0 0.0 0.0 0.0944624709731795 0.0 40-41 0.0 0.0 0.0 0.13356716996042814 0.0 42-43 0.0 0.0 0.0 0.1727497279800058 0.0 44-45 0.0 0.0 0.0 0.22129483463714772 0.0 46-47 0.0 0.0 0.0 0.2800784040455553 0.0 48-49 0.0 0.0 0.0 0.3455578502342584 0.0 50-51 0.0 0.0 0.0 0.4155725850561266 0.0 52-53 0.0 0.0 0.0 0.485061771409774 0.0 54-55 0.0 0.0 0.0 0.5628818742226263 0.0 56-57 0.0 0.0 0.0 0.6539380125314113 0.0 58-59 0.0 0.0 0.0 0.7475829636651361 0.0 60-61 0.0 0.0 0.0 0.8432133201232508 0.0 62-63 0.0 0.0 0.0 0.9399726325501363 0.0 64-65 0.0 0.0 0.0 1.037821971429628 0.0 66-67 0.0 0.0 0.0 1.1465131805609352 0.0 68-69 0.0 0.0 0.0 1.2703479714802364 0.0 70-71 0.0 0.0 0.0 1.429375045012253 0.0 72-73 0.0 0.0 0.0 1.6014045769432155 0.0 74-75 0.0 0.0 0.0 1.7811226883166682 0.0 76-77 0.0 0.0 0.0 1.991789765040905 0.0 78-79 0.0 0.0 0.0 2.2385250384915594 0.0 80-81 3.892951616450835E-5 0.0 0.0 2.5074890656721474 0.0 82-83 3.892951616450835E-5 0.0 0.0 2.7857183176998883 0.0 84-85 3.892951616450835E-5 0.0 0.0 3.068580182151206 0.0 86-87 3.892951616450835E-5 1.9464758082254176E-5 0.0 3.362712141532149 0.0 88-89 3.892951616450835E-5 3.892951616450835E-5 0.0 3.6753745506073976 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 13690 0.0 33.800716 1 GTATCAA 19935 0.0 29.259064 1 GTACATG 16185 0.0 23.869123 1 ATCAACG 25065 0.0 23.24268 3 TACATGG 16420 0.0 23.002766 2 TATCAAC 25400 0.0 22.903238 2 TCAACGC 25400 0.0 22.864094 4 CAACGCA 25540 0.0 22.73829 5 GAGTACT 13985 0.0 22.60808 12-13 AACGCAG 26045 0.0 22.370213 6 ACATGGG 17010 0.0 21.308092 3 GTACTTT 14815 0.0 21.197496 14-15 AGTACTT 14325 0.0 21.144945 12-13 ACGCAGA 29270 0.0 19.775867 7 CATGGGA 10580 0.0 19.7321 4 CGCAGAG 29460 0.0 19.600048 8 AGAGTAC 25290 0.0 19.016056 10-11 ACTTTTT 17295 0.0 18.952738 16-17 GCAGAGT 29640 0.0 18.88923 9 TACTTTT 16325 0.0 18.598 14-15 >>END_MODULE