FastQCFastQC Report
Fri 27 May 2016
SRR939515_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939515_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3013054
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90030.2987998223729147No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74060.24579712145882548No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46710.15502543266732027No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62100.053.3178521
GTATCAA113250.042.927371
TATCAAC146200.033.0693052
ATCAACG149500.032.1949923
TCAACGC151650.031.5882114
CAACGCA153700.030.9189515
AACGCAG158150.030.3401876
ACGCAGA177500.026.3637857
CGCAGAG178500.026.1475548
GTGGTAT31300.025.726921
GCAGAGT183900.024.3147629
TACATGG113600.023.4054492
GTACATG115750.023.3334521
TGGTATC33750.022.8392662
GAGTACT107850.022.30844712-13
ACATGGG118800.021.8094273
AGAGTAC170950.020.75111210-11
GTACTTT117200.020.71228814-15
CAGAGTA178400.020.62660610-11
AGTACTT112350.020.0511812-13
Produced by FastQC (version 0.11.5)