FastQCFastQC Report
Fri 27 May 2016
SRR939515_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939515_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3013054
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121490.40321215617111406No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118200.3922930023823005No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77260.25641757499201806No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33130.10995488298583429No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32410.10756528094086598No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72000.052.66351
GTATCAA122700.037.8172871
ATCAACG157100.029.2489243
TATCAAC157650.029.2142492
TCAACGC159900.028.707124
CAACGCA161800.028.340745
GTGGTAT33450.028.1974051
AACGCAG165850.027.6772296
TGGTATC34100.026.6789842
ACGCAGA191600.023.8586657
CGCAGAG191500.023.7227028
GCAGAGT203150.021.4995029
GAGTACT119200.020.96403912-13
TGCACCG6600.020.8146155
GTACATG125300.020.3887181
TACATGG123300.020.0599522
CAGAGTA201100.019.56361410-11
GTACTTT127600.019.50970814-15
AGTACTT123350.019.06816312-13
AGAGTAC193350.018.97574210-11
Produced by FastQC (version 0.11.5)