##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939515_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3013054 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.96720968160544 31.0 31.0 31.0 30.0 34.0 2 31.229010498982095 31.0 31.0 34.0 30.0 34.0 3 31.219164011000135 31.0 31.0 34.0 28.0 34.0 4 35.160802295611035 35.0 35.0 37.0 33.0 37.0 5 34.77797742755357 35.0 35.0 37.0 32.0 37.0 6 34.731048962282124 35.0 35.0 37.0 32.0 37.0 7 34.604960946601025 35.0 35.0 37.0 30.0 37.0 8 34.617370282776214 35.0 35.0 37.0 30.0 37.0 9 35.97128328931377 37.0 35.0 39.0 30.0 39.0 10-11 35.83006295273832 37.0 35.0 39.0 30.0 39.0 12-13 35.78943639908213 37.0 35.0 39.0 30.0 39.0 14-15 36.66380887962845 38.0 35.5 40.0 31.0 41.0 16-17 36.66325860737976 38.0 35.5 40.0 31.0 41.0 18-19 36.604884612091254 38.0 35.0 40.0 30.0 41.0 20-21 36.49833275473988 38.0 35.0 40.0 30.0 41.0 22-23 36.35897448236905 38.0 34.5 40.0 30.0 41.0 24-25 36.14585135214968 38.0 34.0 40.0 29.0 41.0 26-27 35.77857184106226 38.0 34.0 40.0 27.5 41.0 28-29 35.48655500366074 38.0 34.0 40.0 27.0 41.0 30-31 35.26811899156139 38.0 34.0 40.0 27.0 41.0 32-33 35.08080505692895 37.5 33.0 40.0 27.0 41.0 34-35 35.05675122317754 38.0 33.0 40.0 26.5 41.0 36-37 35.071078712827585 38.0 33.5 40.0 26.5 41.0 38-39 35.04912706509741 38.0 33.0 40.0 26.0 41.0 40-41 34.831256426204106 38.0 33.0 40.0 25.5 41.0 42-43 34.541149610992704 38.0 33.0 40.0 24.5 41.0 44-45 34.37074692322142 37.0 33.0 40.0 24.0 41.0 46-47 34.06756002381637 37.0 33.0 40.0 24.0 41.0 48-49 33.77743263147624 37.0 32.5 40.0 23.0 41.0 50-51 33.52005357355029 37.0 32.0 40.0 23.0 41.0 52-53 33.138228687570816 37.0 31.0 39.5 21.0 41.0 54-55 32.91021319232911 36.0 31.0 39.0 20.5 40.5 56-57 32.520231963980734 36.0 31.0 39.0 19.0 40.0 58-59 32.083198475699405 35.5 30.0 39.0 17.5 40.0 60-61 31.792719446780573 35.0 30.0 39.0 16.5 40.0 62-63 31.39608549996117 35.0 29.0 38.0 13.0 40.0 64-65 30.953026231856448 34.5 29.0 38.0 11.0 40.0 66-67 29.219533901483345 33.5 25.5 37.5 7.0 39.5 68-69 25.499995021662407 30.0 16.0 35.0 4.5 39.0 70-71 24.87782844250385 29.5 15.0 35.0 2.0 38.0 72-73 26.736286339375265 31.0 21.5 35.0 3.5 38.0 74-75 27.36630276125154 32.0 24.0 35.0 2.0 37.5 76-77 27.46238998703641 31.5 25.5 34.5 2.0 36.5 78-79 28.311273876936824 33.0 26.5 35.0 2.0 37.0 80-81 28.17149709231896 33.0 26.0 35.0 2.0 36.5 82-83 27.75887770348623 33.0 26.0 35.0 2.0 36.0 84-85 27.42191593645517 32.0 26.0 35.0 2.0 36.0 86-87 26.97131929928903 32.0 25.5 34.5 2.0 35.0 88-89 26.480941596134684 32.0 25.0 34.0 2.0 35.0 90-91 26.03199892866175 32.0 24.0 34.0 2.0 35.0 92-93 25.456000954513264 31.5 21.5 34.0 2.0 35.0 94-95 24.04467045728354 31.0 15.0 34.0 2.0 35.0 96-97 23.227542719114894 30.0 4.5 34.0 2.0 35.0 98-99 22.875356863833176 30.0 2.0 34.0 2.0 35.0 100-101 21.609202822120018 29.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.39832582050040344 1101 2 0.30443524694124235 1101 3 0.37302154513909613 1101 4 0.3294374066924277 1101 5 0.3730286590469305 1101 6 0.5372532288510286 1101 7 0.5119770586027883 1101 8 0.5314758921486131 1101 9 0.5758963459758846 1101 10-11 0.7955287365850623 1101 12-13 0.6046712372147098 1101 14-15 0.7271949773731805 1101 16-17 0.7444523225992441 1101 18-19 0.7516820094000352 1101 20-21 0.8347891298693355 1101 22-23 0.8835450164520111 1101 24-25 0.7990091322009008 1101 26-27 0.9324580552867943 1101 28-29 0.7542874751610356 1101 30-31 0.6947151921818246 1101 32-33 0.6855765572837313 1101 34-35 0.7054644033434556 1101 36-37 0.7267198141847544 1101 38-39 0.7210477054493438 1101 40-41 0.8222430397976837 1101 42-43 0.7465337176346694 1101 44-45 0.522745961430978 1101 46-47 0.6597713634890283 1101 48-49 0.5604092908722222 1101 50-51 0.5878878794537599 1101 52-53 0.5899207395423289 1101 54-55 0.5357698306614012 1101 56-57 0.4144435006613989 1101 58-59 0.35468260538635477 1101 60-61 0.23965386681885192 1101 62-63 0.16827050507804842 1101 64-65 -0.08916966420617811 1101 66-67 -3.2684921663787705 1101 68-69 -8.03925700654493 1101 70-71 -11.556668179454512 1101 72-73 -11.446660845966687 1101 74-75 -9.874479582915157 1101 76-77 -4.954599030504344 1101 78-79 -1.9593103103852378 1101 80-81 -1.383465903097676 1101 82-83 -0.8616738921071772 1101 84-85 -1.013768143960462 1101 86-87 -0.921583073368911 1101 88-89 -0.7796456409256081 1101 90-91 -0.9217807387886658 1101 92-93 -1.4306756803322003 1101 94-95 -5.117516503939264 1101 96-97 -1.9321365055327604 1101 98-99 -1.2522917558302105 1101 100-101 -1.0172863365654052 1102 1 0.4336743100885485 1102 2 0.3033148796648213 1102 3 0.3558410102208214 1102 4 0.2569456359309257 1102 5 0.4261159565152397 1102 6 0.4453863114562324 1102 7 0.5245956094036757 1102 8 0.4698025484828179 1102 9 0.7118883335769226 1102 10-11 0.6907566225061359 1102 12-13 0.5931609675516256 1102 14-15 0.4286464248660167 1102 16-17 0.5981266420429989 1102 18-19 0.7103096905677049 1102 20-21 0.827804674776452 1102 22-23 0.7707733449390375 1102 24-25 0.8490286833075018 1102 26-27 0.8065345765406278 1102 28-29 0.6640480540870826 1102 30-31 0.48472017771401 1102 32-33 0.4368702628938408 1102 34-35 0.5899894358693842 1102 36-37 0.8369947205171755 1102 38-39 0.6841424145644268 1102 40-41 0.49588525987915943 1102 42-43 0.7225001199466803 1102 44-45 0.6739003654066096 1102 46-47 0.5927488370594887 1102 48-49 0.5122593143335408 1102 50-51 0.3732737383161009 1102 52-53 0.4501839152108005 1102 54-55 0.2862311923426475 1102 56-57 0.41894438757068286 1102 58-59 0.39663150412583903 1102 60-61 0.3169257054049197 1102 62-63 0.1787008871422202 1102 64-65 0.015981830209312875 1102 66-67 -3.1988970253619726 1102 68-69 -7.951836277395298 1102 70-71 -11.261953732253165 1102 72-73 -9.545826191452516 1102 74-75 -8.005711853616226 1102 76-77 -4.333875479596816 1102 78-79 -1.4964601234055657 1102 80-81 -0.8011391703364232 1102 82-83 -0.6183055830290627 1102 84-85 -0.6810830483022272 1102 86-87 -0.6291755234424947 1102 88-89 -0.5596961361018167 1102 90-91 -0.6400321855705542 1102 92-93 -1.0057697478155099 1102 94-95 -4.7450587698769695 1102 96-97 -1.4215948154640117 1102 98-99 -0.6371607545291731 1102 100-101 -0.3433800876762625 1103 1 0.3892360598762039 1103 2 0.23500327857531644 1103 3 0.3431655628643533 1103 4 0.1650662010497399 1103 5 0.4724053066549345 1103 6 0.44772070780758355 1103 7 0.4818429706973788 1103 8 0.4139157478305222 1103 9 0.6050605095740806 1103 10-11 0.7086554902053805 1103 12-13 0.5229449327332105 1103 14-15 0.674782358020984 1103 16-17 0.7291069963286887 1103 18-19 0.5814712480902884 1103 20-21 0.7199321228089204 1103 22-23 0.6824189890160213 1103 24-25 0.7142155381715156 1103 26-27 0.7488956617408249 1103 28-29 0.8756579228156909 1103 30-31 0.9325577444635655 1103 32-33 0.831039539551405 1103 34-35 0.846830318971783 1103 36-37 0.7482373980935009 1103 38-39 0.7465434013494914 1103 40-41 0.7840289143639154 1103 42-43 0.7990863615817219 1103 44-45 0.5494958713205165 1103 46-47 0.6902171275904365 1103 48-49 0.5789221766686907 1103 50-51 0.4764052884421872 1103 52-53 0.5518565773576256 1103 54-55 0.5778448361387021 1103 56-57 0.596752917028283 1103 58-59 0.5792444653370232 1103 60-61 0.5380193336617367 1103 62-63 0.606119948160039 1103 64-65 0.4859900168381124 1103 66-67 -2.7806509392509255 1103 68-69 -7.545587443386328 1103 70-71 -11.49757302987114 1103 72-73 -9.479147965470833 1103 74-75 -7.931839929363392 1103 76-77 -4.179729160616791 1103 78-79 -1.1166835108262525 1103 80-81 -0.37111860499812366 1103 82-83 -0.06442681097949077 1103 84-85 -0.2582866890474982 1103 86-87 0.03243686931376644 1103 88-89 0.1281525363693845 1103 90-91 0.19896821168945067 1103 92-93 0.2054029154287207 1103 94-95 1.0320035253201354 1103 96-97 1.0815356372587566 1103 98-99 0.7993935307210727 1103 100-101 0.6788658140710204 1104 1 0.355147082666889 1104 2 0.1428714235488293 1104 3 0.240709100959279 1104 4 0.18829531325210525 1104 5 0.46124342449175515 1104 6 0.5027335637650125 1104 7 0.5247490195275901 1104 8 0.35558791497951603 1104 9 0.5897637891514691 1104 10-11 0.4955121151680615 1104 12-13 0.5759994264257244 1104 14-15 0.695719649004495 1104 16-17 0.6844884908738678 1104 18-19 0.7049168469641884 1104 20-21 0.8411254775519907 1104 22-23 0.6164636838612836 1104 24-25 0.6065019637140452 1104 26-27 0.7847668831733188 1104 28-29 0.8883291431927844 1104 30-31 0.7553128990494713 1104 32-33 0.6569122693106948 1104 34-35 0.773326167994874 1104 36-37 0.7696482069480339 1104 38-39 0.7757862960528925 1104 40-41 0.9234649320645474 1104 42-43 0.9081734368920777 1104 44-45 0.7794623671167713 1104 46-47 0.8063007724467539 1104 48-49 0.82931911287438 1104 50-51 0.8165961291606791 1104 52-53 0.8222147016323262 1104 54-55 0.6160663649312497 1104 56-57 0.6572550271466966 1104 58-59 0.7478391664926285 1104 60-61 0.6066040086217086 1104 62-63 0.672034005194142 1104 64-65 0.4445637553294226 1104 66-67 -2.699426617635197 1104 68-69 -7.457818918340848 1104 70-71 -8.934498716852431 1104 72-73 -8.228205393144805 1104 74-75 -7.24882496438692 1104 76-77 -3.7436657704346317 1104 78-79 -0.6208611802898467 1104 80-81 0.16995761915419294 1104 82-83 0.3920653925540414 1104 84-85 0.4518435063797668 1104 86-87 0.5421500541567958 1104 88-89 0.5129861125154811 1104 90-91 0.4871172924236298 1104 92-93 0.625401481858642 1104 94-95 1.4900240640698428 1104 96-97 1.849191437962613 1104 98-99 1.4609128159764495 1104 100-101 1.4509215755495894 1105 1 0.48077662192546455 1105 2 0.3150701975150909 1105 3 0.5023635745423825 1105 4 0.3342988176032833 1105 5 0.39463286085625526 1105 6 0.5197852865431472 1105 7 0.5733324365849413 1105 8 0.4712094481421687 1105 9 0.7549602972269511 1105 10-11 0.7475902544583377 1105 12-13 0.7881328197068029 1105 14-15 0.944727340228873 1105 16-17 0.8730232105459379 1105 18-19 0.9396223845753795 1105 20-21 0.9961319564909132 1105 22-23 1.0191119517545744 1105 24-25 0.9488323786999686 1105 26-27 0.9868573847544937 1105 28-29 1.1987142141796312 1105 30-31 1.0732289266115842 1105 32-33 1.0975988900851306 1105 34-35 1.098871887402538 1105 36-37 1.128604294082571 1105 38-39 0.9784872313389386 1105 40-41 1.1832604706720957 1105 42-43 1.3138149153516991 1105 44-45 1.2623212633877543 1105 46-47 1.2090465741380996 1105 48-49 1.0543751122070475 1105 50-51 1.051112177439613 1105 52-53 1.4343413666689955 1105 54-55 1.2559006527341339 1105 56-57 1.379620586911308 1105 58-59 1.413568184217862 1105 60-61 1.5076117949340357 1105 62-63 1.421661484442783 1105 64-65 1.291272721255762 1105 66-67 2.1133450062686414 1105 68-69 -5.0368775065028615 1105 70-71 -5.595326714862452 1105 72-73 -1.597348205367311 1105 74-75 1.2944304383118137 1105 76-77 1.4059871628610878 1105 78-79 1.2452890650121624 1105 80-81 1.2873076417375735 1105 82-83 1.4110037699881275 1105 84-85 1.4076057714157812 1105 86-87 1.5843734351322247 1105 88-89 1.5387219477373435 1105 90-91 1.5175593372229272 1105 92-93 1.6566289891187544 1105 94-95 2.416327946773201 1105 96-97 2.409221624093828 1105 98-99 2.1812965902314936 1105 100-101 1.8896762290016618 1106 1 0.13944639153583438 1106 2 0.16412541487444443 1106 3 0.21931536569762144 1106 4 0.14838930962265806 1106 5 0.2721749204222874 1106 6 0.23293684520027824 1106 7 0.22391649945090109 1106 8 0.21369475257954917 1106 9 0.3911424603706024 1106 10-11 0.3578850786434131 1106 12-13 0.2249764565911363 1106 14-15 0.31277699726248187 1106 16-17 0.1915070416160347 1106 18-19 0.14993211885370528 1106 20-21 0.2800603404269282 1106 22-23 0.3934623007500946 1106 24-25 0.42526394034425863 1106 26-27 0.4268612591520764 1106 28-29 0.5734182175696461 1106 30-31 0.5148290630890457 1106 32-33 0.5110126128861978 1106 34-35 0.5881906597152238 1106 36-37 0.5830442817708317 1106 38-39 0.5212967125481995 1106 40-41 0.6064893340091402 1106 42-43 0.6484915701536664 1106 44-45 0.503837839968682 1106 46-47 0.4496848169086718 1106 48-49 0.3719838210032478 1106 50-51 0.35700220381189496 1106 52-53 0.5969629104776928 1106 54-55 0.7364482479613272 1106 56-57 0.49192985804326383 1106 58-59 0.7530284868011243 1106 60-61 0.7022480751560991 1106 62-63 0.6603009097439063 1106 64-65 0.6136196452975256 1106 66-67 1.3150551879696657 1106 68-69 -5.469755880735757 1106 70-71 -6.600903098302435 1106 72-73 -2.586769361462199 1106 74-75 0.22084869991312317 1106 76-77 0.5731103387297942 1106 78-79 0.4642431420962332 1106 80-81 0.5187304020591554 1106 82-83 0.4653081965815673 1106 84-85 0.4626582719009349 1106 86-87 0.4959738816299968 1106 88-89 0.5216001763805842 1106 90-91 0.43428475359648644 1106 92-93 0.6251173505962804 1106 94-95 1.5066176393512265 1106 96-97 1.5539413785714373 1106 98-99 1.3030536877698786 1106 100-101 1.0896977848343532 1107 1 -0.05933159941997346 1107 2 -0.07224039862652631 1107 3 -0.0877990655479266 1107 4 -0.06670215282399283 1107 5 0.11035946002577646 1107 6 0.12765846644187917 1107 7 0.026803111416988656 1107 8 0.010045507547275179 1107 9 0.13013408292756168 1107 10-11 0.006972041672902662 1107 12-13 0.06730318140551361 1107 14-15 0.11596990523760553 1107 16-17 0.0025058033691749415 1107 18-19 0.13253307225843258 1107 20-21 0.12984090312620822 1107 22-23 0.11004287977791449 1107 24-25 0.32133134995903845 1107 26-27 0.17332381919344186 1107 28-29 0.30478169518368503 1107 30-31 0.3887856019849636 1107 32-33 0.25101270360025296 1107 34-35 0.185537908584692 1107 36-37 0.3190545415305266 1107 38-39 0.21711248137249584 1107 40-41 0.23744305623363005 1107 42-43 0.3439199899601775 1107 44-45 0.28435577186963457 1107 46-47 0.058493650643413275 1107 48-49 0.2567044897472712 1107 50-51 0.1116855437219968 1107 52-53 0.24583923546986597 1107 54-55 0.1380617788698757 1107 56-57 0.14834334178050312 1107 58-59 0.23040190667566307 1107 60-61 0.10454137184095202 1107 62-63 0.20834643005704834 1107 64-65 0.3117376461411645 1107 66-67 1.0702072474055129 1107 68-69 -5.412732571759147 1107 70-71 -6.027199243408635 1107 72-73 -2.3576356806940346 1107 74-75 0.020775448313152367 1107 76-77 0.007717252047978462 1107 78-79 0.1843193419030129 1107 80-81 0.1581621238572879 1107 82-83 0.16949338025485616 1107 84-85 0.3164755647213724 1107 86-87 0.3870807291804894 1107 88-89 0.2287298475874877 1107 90-91 0.23734993618120015 1107 92-93 0.37928747573632293 1107 94-95 1.1969713334543606 1107 96-97 1.4460587353452858 1107 98-99 1.1226672912496731 1107 100-101 0.8071204624411301 1108 1 0.04920339304794297 1108 2 0.04331408036784268 1108 3 0.03626950524789052 1108 4 0.015009487186247839 1108 5 0.1711354607086264 1108 6 0.26009607010895763 1108 7 0.20994148720912875 1108 8 0.1628503262351515 1108 9 0.296752073649138 1108 10-11 0.3436591706918435 1108 12-13 0.33184563905628295 1108 14-15 0.22599415093347375 1108 16-17 0.11298557286204414 1108 18-19 0.25652629915062874 1108 20-21 0.3786103706206063 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-2.5996409187042353 2316 44-45 -0.21233280317935765 2316 46-47 -0.22840525364515685 2316 48-49 -0.0803815054033663 2316 50-51 0.4332713656809446 2316 52-53 -2.0080879187934997 2316 54-55 -0.39631295531378186 2316 56-57 0.7253154250352765 2316 58-59 0.8249693878547681 2316 60-61 -0.7362258453852633 2316 62-63 -0.14993664845797738 2316 64-65 0.9836497261513379 2316 66-67 1.8495488559397018 2316 68-69 4.304587034854581 2316 70-71 4.413744936254712 2316 72-73 2.7618070367053704 2316 74-75 1.6953307403055575 2316 76-77 0.44741945004772177 2316 78-79 0.7537068571943024 2316 80-81 0.5248731042098989 2316 82-83 0.5482498201354176 2316 84-85 0.9017699686569927 2316 86-87 0.5338310832369011 2316 88-89 1.1161442930891106 2316 90-91 1.3057304843438367 2316 92-93 1.4637072397571735 2316 94-95 1.8945755882697455 2316 96-97 2.0258822113681028 2316 98-99 0.9293619608920203 2316 100-101 1.0498117296342464 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 18.0 3 31.0 4 85.0 5 227.0 6 485.0 7 1279.0 8 2810.0 9 4610.0 10 7115.0 11 10610.0 12 14125.0 13 17897.0 14 21917.0 15 27053.0 16 32520.0 17 36373.0 18 34858.0 19 30896.0 20 30303.0 21 31215.0 22 33027.0 23 37556.0 24 45711.0 25 56878.0 26 72240.0 27 90404.0 28 110829.0 29 132171.0 30 155710.0 31 180737.0 32 207959.0 33 241041.0 34 281026.0 35 321695.0 36 341671.0 37 282370.0 38 111604.0 39 5997.0 40 1.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.30492588024984 20.123956173824133 15.412592473330665 23.15852547259536 2 16.17239883246589 20.992875359792173 38.85244263657165 23.982283171170284 3 19.438782046388813 24.48313239656508 30.34193877706805 25.736146779978053 4 13.551565952684552 17.708245520989667 36.02079484801799 32.71939367830779 5 13.812823426643053 37.44829156389145 33.9731394919696 14.765745517495905 6 32.17280539943857 36.312790942346204 16.92269703762362 14.5917066205916 7 28.337693250768158 32.948098507361635 20.21566822234185 18.49854001952836 8 26.3560161882263 35.50878942096624 19.03507205645833 19.100122334349134 9 25.90378400121604 16.95489028739611 20.125792634317207 37.01553307707064 10-11 25.12507243481199 26.95124282538581 26.764422409953486 21.15926232984872 12-13 25.91007330104273 24.13984283056328 27.546486057003953 22.40359781139004 14-15 22.81178830515484 25.556710898643036 25.981628606722612 25.64987218947951 16-17 22.17522473112748 28.844474609572707 26.632824932916698 22.347475726383113 18-19 21.90671027294192 26.996102252157467 29.39840797710737 21.698779497793243 20-21 22.77383677823232 26.1057551573918 29.52852819763602 21.59187986673986 22-23 22.442644572583166 26.179517526071557 29.30986301606277 22.067974885282506 24-25 22.350196843468453 26.295380036335224 29.424779642183648 21.929643478012675 26-27 22.13850830697706 26.60026528029254 29.206288201664798 22.054938211065604 28-29 21.962915807959675 26.62666365922125 29.462077225289697 21.948343307529385 30-31 22.17360942393502 26.439647996431376 29.304921066711277 22.081821512922332 32-33 21.996953015701262 26.44325987490105 29.222620705220866 22.337166404176827 34-35 22.498141663725026 26.54751407796565 29.036680628875267 21.917663629434056 36-37 22.394619894775204 26.394102308640132 29.0080617860974 22.203216010487264 38-39 22.232869885882295 26.621036195412035 29.14137638909653 22.004717529609145 40-41 22.406822095388723 26.40998527228823 29.21288562640123 21.970307005921818 42-43 22.303795113378328 26.474001305623986 28.91003652757175 22.312167053425934 44-45 22.357852137720073 26.761074788227862 28.687031030900677 22.194042043151384 46-47 22.421475428270924 26.79435437991347 28.2958377745476 22.488332417268005 48-49 22.547945929074725 26.961660698116845 28.197456980844553 22.292936391963877 50-51 22.45830083199009 27.13083678722395 27.9874271294415 22.423435251344458 52-53 22.838403769871256 26.99366850016557 27.826282483271164 22.341645246692014 54-55 22.46368677606851 26.983370539346602 28.03427713288093 22.518665551703958 56-57 22.494892040614005 27.378745311202053 27.820466764897077 22.305895883286865 58-59 22.60859549128652 26.97870521082759 27.880849560894454 22.53184973699144 60-61 22.609887242071817 26.901652177564156 28.18252833801588 22.305932242348142 62-63 22.570203309061835 26.927821748364337 28.053494209589847 22.44848073298398 64-65 22.4915173286132 26.829653221954743 28.04990329937167 22.62892615006039 66-67 22.001519213821847 27.093351989643484 28.474743320192147 22.430385476342526 68-69 22.089076333790977 26.581842420243245 29.064883203674896 22.264198042290882 70-71 21.566704547471748 26.594753424763063 29.38105242991465 22.45748959785054 72-73 22.357159807348665 26.871838804446117 28.110123846809508 22.660877541395706 74-75 22.14966303854129 27.032001990308714 27.975936143929765 22.84239882722023 76-77 22.335190086194448 27.101726179352305 27.752076523755008 22.811007210698243 78-79 22.24765096249257 27.222430870660336 27.665776070677662 22.86414209616943 80-81 22.098392234721384 27.2964306030995 27.656237865092688 22.948939297086426 82-83 22.261549245383588 27.349924694346665 27.488787124293157 22.899738935976586 84-85 22.20726877115379 27.168066022049388 27.447599677934747 23.17706552886208 86-87 22.207832939766913 27.0652114389264 27.72358195778689 23.003373663519795 88-89 22.139005894563628 27.12229431464434 27.49680097828416 23.241898812507873 90-91 21.99732222039308 27.286799887419082 27.65411504495271 23.06176284723513 92-93 22.130897667691947 27.102002072683312 27.602399931629662 23.16470032799508 94-95 21.60740718564866 27.018015680560957 27.981036300798003 23.39354083299238 96-97 21.55295927304977 26.90633656377231 27.970691525511686 23.57001263766623 98-99 21.75351855540065 26.844519683993894 27.594735444466572 23.807226316138884 100-101 21.63435551707548 26.806761584558224 27.811785292456605 23.74709760590969 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2706.0 1 1814.5 2 941.0 3 977.0 4 926.5 5 979.0 6 1503.5 7 2790.5 8 4092.5 9 4887.0 10 4716.0 11 3965.0 12 3813.0 13 3901.5 14 3919.0 15 4051.0 16 4315.5 17 4626.5 18 4980.5 19 5337.0 20 5964.5 21 6807.5 22 7900.5 23 9304.0 24 11088.0 25 13519.5 26 16843.0 27 20897.5 28 24822.5 29 29054.0 30 33786.0 31 38701.0 32 43828.0 33 48863.0 34 54453.0 35 59824.5 36 65606.5 37 71775.0 38 76701.0 39 79737.5 40 82917.5 41 88846.5 42 94471.0 43 98900.5 44 103961.0 45 107743.0 46 109530.0 47 112826.5 48 119156.0 49 122282.5 50 120749.5 51 118311.5 52 113686.0 53 110348.0 54 106479.0 55 98302.0 56 89195.5 57 80062.5 58 71078.5 59 62026.0 60 52066.5 61 41602.0 62 32156.5 63 24389.0 64 18268.0 65 13707.0 66 10129.0 67 7426.5 68 5295.5 69 3586.0 70 2568.0 71 1890.0 72 1508.5 73 1261.0 74 997.0 75 763.0 76 498.0 77 278.5 78 164.0 79 103.5 80 60.5 81 35.5 82 27.5 83 18.0 84 6.5 85 3.0 86 3.0 87 2.0 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023199053186567516 2 0.018287093427465952 3 0.0 4 0.0 5 1.9913350374736066E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.98700255621041E-4 18-19 0.0010620453533192568 20-21 0.0 22-23 0.0 24-25 0.0 26-27 4.8123930072278826E-4 28-29 0.017606720622995804 30-31 0.00431455924785948 32-33 0.007932151232603199 34-35 0.009243113465606656 36-37 0.09714396091142077 38-39 0.09969950754284525 40-41 0.11355588051193241 42-43 0.0995335629563891 44-45 0.0949534923701998 46-47 0.09616488785132958 48-49 0.09858767881358912 50-51 0.09697801632496464 52-53 0.07417723014589184 54-55 0.06279343151500107 56-57 0.03610954201285473 58-59 0.016909753359880042 60-61 0.009043979961859296 62-63 0.0018253904510174726 64-65 0.004878770841810336 66-67 0.4254155418389448 68-69 0.8151529975898208 70-71 0.9084802330127505 72-73 0.2806122956973224 74-75 0.1485369993368854 76-77 0.014835446029178367 78-79 0.009209924548315429 80-81 0.0017258236991437924 82-83 0.0 84-85 0.0 86-87 2.98700255621041E-4 88-89 7.799395563438292E-4 90-91 0.003650780902034945 92-93 0.0017092292404981791 94-95 0.22515361490368244 96-97 0.23102805326422957 98-99 0.015067768450216956 100-101 0.007948745691248814 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3013054.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.44632859521761 #Duplication Level Percentage of deduplicated Percentage of total 1 73.15277642141112 39.09747326271226 2 14.858820504679237 15.882988064608874 3 5.328334629617758 8.543397704394835 4 2.4410524481047022 5.218611650382572 5 1.2469470515389516 3.3322370928694287 6 0.7441335884281667 2.3862724971522127 7 0.4719635594754895 1.7657303639286883 8 0.3241279657142233 1.3858759809969445 9 0.21946012638248366 1.0556404225367588 >10 1.0772968700501393 10.29867797093093 >50 0.08085922720948716 2.9632252619765675 >100 0.049972367018211 5.035826654762943 >500 0.003066276149176909 1.1561986070651313 >1k 0.001001233028044667 0.8178006460692759 >5k 6.25770642527917E-5 0.2583971777985943 >10k+ 1.251541285055834E-4 0.8016466418139406 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12149 0.40321215617111406 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11820 0.3922930023823005 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7726 0.25641757499201806 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3313 0.10995488298583429 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3241 0.10756528094086598 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 6.637783458245355E-5 0.0 0.0 0.0 0.0 6 9.956675187368033E-5 0.0 0.0 0.0 0.0 7 1.327556691649071E-4 0.0 0.0 0.0 0.0 8 1.327556691649071E-4 0.0 0.0 0.0 0.0 9 1.327556691649071E-4 0.0 0.0 0.0 0.0 10-11 1.327556691649071E-4 0.0 0.0 3.3188917291226776E-5 0.0 12-13 1.6594458645613388E-4 0.0 0.0 3.3188917291226776E-5 0.0 14-15 1.9913350374736066E-4 0.0 0.0 3.3188917291226776E-5 0.0 16-17 1.9913350374736066E-4 0.0 0.0 3.3188917291226776E-5 0.0 18-19 2.489168796842008E-4 0.0 0.0 4.9783375936840164E-5 0.0 20-21 2.821057969754276E-4 0.0 0.0 1.8253904510174727E-4 0.0 22-23 2.98700255621041E-4 0.0 0.0 5.14428218014015E-4 0.0 24-25 3.8167254884910793E-4 0.0 0.0 9.458841427999632E-4 0.0 26-27 3.982670074947213E-4 0.0 0.0 0.001194801022484164 0.0 28-29 4.314559247859481E-4 0.0 0.0 0.0017258236991437924 0.0 30-31 4.314559247859481E-4 0.0 0.0 0.004314559247859481 0.0 32-33 4.480503834315615E-4 0.0 0.0 0.010670236909129409 0.0 34-35 4.6464484207717487E-4 0.0 0.0 0.020012917126609745 0.0 36-37 4.978337593684016E-4 0.0 0.0 0.0366903480654512 0.0 38-39 5.642115939508552E-4 0.0 0.0 0.061333119154187085 0.0 40-41 5.642115939508552E-4 0.0 0.0 0.08584313457375806 0.0 42-43 6.969672631157623E-4 0.0 0.0 0.11226151273757456 0.0 44-45 9.292896841543497E-4 0.0 0.0 0.14704349805878025 1.6594458645613388E-5 46-47 0.0010122619773824167 0.0 0.0 0.18994017365769084 3.3188917291226776E-5 48-49 0.0010620453533192568 0.0 0.0 0.2368527082488399 3.3188917291226776E-5 50-51 0.0010620453533192568 0.0 0.0 0.2859225224639187 3.3188917291226776E-5 52-53 0.0010620453533192568 0.0 0.0 0.3356893039421132 3.3188917291226776E-5 54-55 0.0010620453533192568 0.0 0.0 0.3950310880588267 3.3188917291226776E-5 56-57 0.0010620453533192568 0.0 0.0 0.46217226773897846 3.3188917291226776E-5 58-59 0.0010620453533192568 3.3188917291226776E-5 0.0 0.5284007521936215 3.3188917291226776E-5 60-61 0.00107863981196487 3.3188917291226776E-5 0.0 0.5942309696407697 4.9783375936840164E-5 62-63 0.0011284231879017104 3.3188917291226776E-5 0.0 0.6622350611704935 6.637783458245355E-5 64-65 0.0011284231879017104 3.3188917291226776E-5 0.0 0.7354332182562942 6.637783458245355E-5 66-67 0.0011284231879017104 3.3188917291226776E-5 0.0 0.8139581965673366 6.637783458245355E-5 68-69 0.0011284231879017104 3.3188917291226776E-5 0.0 0.9116995579899996 6.637783458245355E-5 70-71 0.0011284231879017104 3.3188917291226776E-5 0.0 1.0402070457416297 6.637783458245355E-5 72-73 0.0011450176465473238 3.3188917291226776E-5 0.0 1.1828032288833854 6.637783458245355E-5 74-75 0.001161612105192937 3.3188917291226776E-5 0.0 1.333563885678783 6.637783458245355E-5 76-77 0.001161612105192937 3.3188917291226776E-5 0.0 1.5063619835555553 6.637783458245355E-5 78-79 0.001161612105192937 3.3188917291226776E-5 0.0 1.707851900430593 6.637783458245355E-5 80-81 0.0012279899397753906 3.3188917291226776E-5 0.0 1.9304997520787879 6.637783458245355E-5 82-83 0.0012611788570666175 3.3188917291226776E-5 0.0 2.158590586162744 6.637783458245355E-5 84-85 0.001327556691649071 3.3188917291226776E-5 0.0 2.386117208652749 6.637783458245355E-5 86-87 0.001410528984877138 3.3188917291226776E-5 0.0 2.6255918413675956 6.637783458245355E-5 88-89 0.0014271234435227513 3.3188917291226776E-5 0.0 2.8897092451711783 6.637783458245355E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7200 0.0 52.6635 1 GTATCAA 12270 0.0 37.817287 1 ATCAACG 15710 0.0 29.248924 3 TATCAAC 15765 0.0 29.214249 2 TCAACGC 15990 0.0 28.70712 4 CAACGCA 16180 0.0 28.34074 5 GTGGTAT 3345 0.0 28.197405 1 AACGCAG 16585 0.0 27.677229 6 TGGTATC 3410 0.0 26.678984 2 ACGCAGA 19160 0.0 23.858665 7 CGCAGAG 19150 0.0 23.722702 8 GCAGAGT 20315 0.0 21.499502 9 GAGTACT 11920 0.0 20.964039 12-13 TGCACCG 660 0.0 20.814615 5 GTACATG 12530 0.0 20.388718 1 TACATGG 12330 0.0 20.059952 2 CAGAGTA 20110 0.0 19.563614 10-11 GTACTTT 12760 0.0 19.509708 14-15 AGTACTT 12335 0.0 19.068163 12-13 AGAGTAC 19335 0.0 18.975742 10-11 >>END_MODULE