FastQCFastQC Report
Fri 27 May 2016
SRR939514_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939514_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2981981
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90340.30295296985460335No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71000.23809675514364445No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47150.15811636626792724No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64750.050.7999231
GTATCAA111400.040.4075931
ATCAACG146500.030.7687193
TATCAAC143250.030.479982
TCAACGC151100.029.8125364
CAACGCA153450.029.3126985
AACGCAG157550.028.6503476
ACGCAGA175650.024.8703967
CGCAGAG176450.024.4279048
GTACATG115000.023.2231851
GTGGTAT34000.022.6327651
TACATGG114050.022.2499962
GCAGAGT178950.022.0007729
GAGTACT101050.021.6189212-13
TGGTATC33400.021.5193312
ACATGGG121400.020.6455523
GTACTTT110950.019.96962414-15
AGTACTT106900.019.89628612-13
AGAGTAC165100.019.63181510-11
CAGAGTA174650.019.61325610-11
Produced by FastQC (version 0.11.5)