FastQCFastQC Report
Fri 27 May 2016
SRR939514_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939514_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2981981
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118830.39849348470027146No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112910.37864091018688584No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74530.24993452339233552No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31590.10593628866179898No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31520.1057015453820799No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69550.052.204771
GTATCAA118000.038.6533621
TATCAAC152950.029.7532792
ATCAACG152600.029.6931463
TCAACGC156150.029.0234434
CAACGCA159850.028.3852945
AACGCAG164150.027.7861426
GTGGTAT33550.026.1712881
TGGTATC33900.024.636482
ACGCAGA192900.023.5465537
CGCAGAG193850.023.431168
GCAGAGT202600.021.6703349
GAGTACT118300.020.8168412-13
GTACATG124600.020.7598571
TACATGG122450.020.4229052
GTACTTT123400.019.91808514-15
AGTACTT121550.019.51924712-13
CAGAGTA200500.019.32980210-11
AGAGTAC191650.019.2824110-11
ACATGGG125000.019.0926113
Produced by FastQC (version 0.11.5)