##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939514_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2981981 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.86718325837757 31.0 31.0 31.0 28.0 34.0 2 31.11916440782151 31.0 31.0 34.0 28.0 34.0 3 31.123962895806514 31.0 31.0 34.0 28.0 34.0 4 35.03934397972355 35.0 35.0 37.0 32.0 37.0 5 34.60383550398208 35.0 35.0 37.0 30.0 37.0 6 34.6636906807924 35.0 35.0 37.0 31.0 37.0 7 34.50328322011441 35.0 35.0 37.0 30.0 37.0 8 34.401976068928676 35.0 35.0 37.0 30.0 37.0 9 35.90254867485742 37.0 35.0 39.0 30.0 39.0 10-11 35.698222423281706 37.0 35.0 39.0 30.0 39.0 12-13 35.68192771852 37.0 35.0 39.0 30.0 39.0 14-15 36.523718293309045 38.0 35.0 40.0 30.0 41.0 16-17 36.43060619769207 38.0 35.0 40.0 30.0 41.0 18-19 36.41320736114683 38.0 35.0 40.0 30.0 41.0 20-21 36.2844414501635 38.0 34.0 40.0 29.5 41.0 22-23 36.12212988613945 38.0 34.0 40.0 29.0 41.0 24-25 35.95921788234063 38.0 34.0 40.0 29.0 41.0 26-27 35.496385288839875 38.0 34.0 40.0 27.0 41.0 28-29 35.27302538144944 38.0 33.5 40.0 27.0 41.0 30-31 34.97405902317956 37.0 33.0 40.0 26.5 41.0 32-33 34.75780714229903 37.0 33.0 40.0 25.5 41.0 34-35 35.02704829440563 38.0 33.0 40.0 26.5 41.0 36-37 34.875943039207826 38.0 33.0 40.0 25.5 41.0 38-39 34.69859147325218 38.0 33.0 40.0 25.0 41.0 40-41 34.53923247666568 37.0 33.0 40.0 25.0 41.0 42-43 34.379930992182715 37.0 33.0 40.0 24.5 41.0 44-45 34.00169367276317 37.0 33.0 40.0 24.0 41.0 46-47 33.619870817419695 37.0 32.0 40.0 23.0 41.0 48-49 33.24337378407173 37.0 31.0 39.5 21.5 41.0 50-51 33.00251946608647 37.0 31.0 39.0 20.5 40.0 52-53 32.886380563793 36.0 31.0 39.0 20.5 40.0 54-55 32.540670949949046 36.0 31.0 39.0 19.0 40.0 56-57 32.03664845617729 35.5 30.0 39.0 17.0 40.0 58-59 31.56524957737826 35.0 29.0 38.5 14.0 40.0 60-61 31.191863730855427 35.0 29.0 38.0 13.0 40.0 62-63 31.02355665579358 34.5 29.0 38.0 12.5 40.0 64-65 30.560360880904337 34.0 28.0 38.0 8.0 40.0 66-67 29.671885736361162 34.0 27.0 37.5 7.5 39.5 68-69 26.218080866377083 31.0 18.5 36.0 2.0 39.0 70-71 24.888068703321718 29.5 13.0 35.0 2.0 38.0 72-73 25.892064704637622 30.5 19.5 35.0 2.0 38.0 74-75 26.64116672775581 31.0 22.5 35.0 2.0 37.0 76-77 26.336339668160193 30.5 23.0 34.0 2.0 36.0 78-79 27.465555447871733 32.0 26.0 35.0 2.0 37.0 80-81 27.487178322061744 32.0 26.0 35.0 2.0 36.0 82-83 27.153947325620116 32.0 25.5 35.0 2.0 36.0 84-85 26.787237242624954 32.0 25.0 34.5 2.0 36.0 86-87 26.33630915153383 32.0 24.5 34.0 2.0 35.0 88-89 25.848446552811705 31.0 23.5 34.0 2.0 35.0 90-91 25.250087609545467 31.0 20.5 34.0 2.0 35.0 92-93 24.667868775823855 31.0 19.0 34.0 2.0 35.0 94-95 23.801939717255074 30.5 12.5 34.0 2.0 35.0 96-97 23.104076618865108 30.0 4.5 34.0 2.0 35.0 98-99 22.2296195046179 30.0 2.0 34.0 2.0 35.0 100-101 20.80631549966281 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.33167417859858617 1101 2 0.2831701060875389 1101 3 0.343382497222926 1101 4 0.31366619849251265 1101 5 0.6041202130062402 1101 6 0.43267274802946787 1101 7 0.6843920391320921 1101 8 0.6801701959016313 1101 9 0.7324130764531205 1101 10-11 0.7317573181449362 1101 12-13 0.7199904919627969 1101 14-15 0.7684608311045196 1101 16-17 0.6863887441059262 1101 18-19 0.5353532525222136 1101 20-21 0.7867580224802495 1101 22-23 0.8296881077417737 1101 24-25 0.7792875366581953 1101 26-27 0.9070297296695173 1101 28-29 0.7835915000374385 1101 30-31 0.5241930713372014 1101 32-33 0.9214574805578764 1101 34-35 0.7964179931715734 1101 36-37 1.0709814709353864 1101 38-39 1.0961045636756737 1101 40-41 0.9669832993791445 1101 42-43 0.5607759886595858 1101 44-45 0.6435636789343917 1101 46-47 0.6563202800702257 1101 48-49 0.6266143130503892 1101 50-51 0.5641479736907868 1101 52-53 0.30026012969585025 1101 54-55 0.32176261094243586 1101 56-57 0.40534233661749397 1101 58-59 0.498546319409769 1101 60-61 0.3494303101550855 1101 62-63 0.14906135269752951 1101 64-65 -0.017107019571678705 1101 66-67 -0.11420265685155329 1101 68-69 -7.096681999167618 1101 70-71 -7.822838042966481 1101 72-73 -4.87873037512437 1101 74-75 -2.849421259254715 1101 76-77 -0.1337129084406108 1101 78-79 0.08385554950167773 1101 80-81 -0.008732780424203668 1101 82-83 -0.2510545077182087 1101 84-85 -0.12723801381104494 1101 86-87 -0.32934577583727176 1101 88-89 -0.2088822768454115 1101 90-91 -0.5198316042558986 1101 92-93 -0.43470092779911695 1101 94-95 -0.08942848787958368 1101 96-97 -0.305786088187336 1101 98-99 -0.3233713191101799 1101 100-101 -0.07399915006813629 1102 1 0.31872351131037746 1102 2 0.27468181363363087 1102 3 0.36070023492925785 1102 4 0.3165408757760275 1102 5 0.48835532659521874 1102 6 0.4568735002126303 1102 7 0.5359847424779431 1102 8 0.6654817497392855 1102 9 0.685575397269254 1102 10-11 0.6492845775211791 1102 12-13 0.5303808755066299 1102 14-15 0.7596832481096456 1102 16-17 0.440105708865687 1102 18-19 0.529606720076238 1102 20-21 0.5669099340185042 1102 22-23 0.6309809907965942 1102 24-25 0.5375495967843662 1102 26-27 0.6673109480189652 1102 28-29 0.6198004513613142 1102 30-31 0.48091718651897253 1102 32-33 0.8068896361198483 1102 34-35 0.5250021193050287 1102 36-37 0.6399789073391204 1102 38-39 0.7362946302906792 1102 40-41 0.6564256704641949 1102 42-43 0.5560278856338456 1102 44-45 0.5545606889665251 1102 46-47 0.4764923665355809 1102 48-49 0.6885276241534228 1102 50-51 0.5435856295204431 1102 52-53 0.36127919853654333 1102 54-55 0.2829292073182401 1102 56-57 0.4297097327361499 1102 58-59 0.10459755765471002 1102 60-61 0.31483624127275434 1102 62-63 0.10931182380668858 1102 64-65 -0.244767803210852 1102 66-67 -0.24179500636819284 1102 68-69 -7.0740850907688575 1102 70-71 -7.862298558902026 1102 72-73 -1.5416368507726084 1102 74-75 0.05172451264350997 1102 76-77 0.5370112126629856 1102 78-79 0.16740069612891162 1102 80-81 0.04577019594196585 1102 82-83 -0.03665126233450522 1102 84-85 -0.30127141884167585 1102 86-87 -0.5792154030970735 1102 88-89 -0.5821795894274295 1102 90-91 -0.5819944648836461 1102 92-93 -0.4619040466777733 1102 94-95 -0.29965767742008254 1102 96-97 -0.3736066538440319 1102 98-99 -0.3824915631190926 1102 100-101 -0.3094531516229857 1103 1 0.39228565863705356 1103 2 0.32294959242764776 1103 3 0.3321142185126007 1103 4 0.2786587964003431 1103 5 0.4062335358952893 1103 6 0.5466421327424271 1103 7 0.46187659923006663 1103 8 0.4417546893588593 1103 9 0.6120561903883441 1103 10-11 0.5282421646812239 1103 12-13 0.5084552483587075 1103 14-15 0.6205869596860936 1103 16-17 0.6949599722540469 1103 18-19 0.6223321284271961 1103 20-21 0.5793517469967355 1103 22-23 0.5460318797544161 1103 24-25 0.566547352285447 1103 26-27 0.6636549857833671 1103 28-29 0.5948488363657134 1103 30-31 0.5065411275024942 1103 32-33 0.7152762403676505 1103 34-35 0.4952808988252215 1103 36-37 0.8512594439751098 1103 38-39 0.8649245474246783 1103 40-41 0.8429961547599447 1103 42-43 0.618467551589788 1103 44-45 0.7998533199511897 1103 46-47 0.7210483061594957 1103 48-49 0.6355059575278261 1103 50-51 0.5708228267450082 1103 52-53 0.45681666253542375 1103 54-55 0.23018638181516593 1103 56-57 0.5991230629724527 1103 58-59 0.7445342648871112 1103 60-61 0.7870726159250623 1103 62-63 0.07593509404957643 1103 64-65 0.10833515130923743 1103 66-67 0.14959470046270695 1103 68-69 -6.9164479966013666 1103 70-71 -7.938541585156212 1103 72-73 -1.1647130010330073 1103 74-75 0.3228414280627909 1103 76-77 0.6659307060309523 1103 78-79 0.3585684352800129 1103 80-81 0.11811134890561803 1103 82-83 0.08653916930213157 1103 84-85 -0.0802982089690687 1103 86-87 -0.05220346202948534 1103 88-89 0.07755877824135027 1103 90-91 -0.04195494517887255 1103 92-93 0.045104531627483624 1103 94-95 0.07654972819428352 1103 96-97 -0.013596628971182412 1103 98-99 3.935800664578437E-4 1103 100-101 0.16590143318457606 1104 1 0.29259733833285395 1104 2 0.31062384873304083 1104 3 0.24091505942312708 1104 4 0.19833539909883768 1104 5 0.5129158021032083 1104 6 0.4115468266774158 1104 7 0.5165163977331986 1104 8 0.5877196542922007 1104 9 0.6566222225428291 1104 10-11 0.5059162486688891 1104 12-13 0.3917505521261475 1104 14-15 0.6999329923575388 1104 16-17 0.6149061804023717 1104 18-19 0.6201402827894213 1104 20-21 0.5069052444722786 1104 22-23 0.6362877787647321 1104 24-25 0.47430818389081963 1104 26-27 0.7143740174151603 1104 28-29 0.7158243198605447 1104 30-31 0.49446991145627095 1104 32-33 0.5889919347793864 1104 34-35 0.25532666354259703 1104 36-37 0.7102606401473608 1104 38-39 0.7064765988672619 1104 40-41 0.6338862548191102 1104 42-43 0.4997675596312803 1104 44-45 0.6515798359164293 1104 46-47 0.5839747566440039 1104 48-49 0.7674072826511775 1104 50-51 0.6599660199074364 1104 52-53 0.44593605431126804 1104 54-55 0.5619813007396743 1104 56-57 0.3706877295089299 1104 58-59 0.5564345632839824 1104 60-61 0.7473749186643417 1104 62-63 0.35124372228555956 1104 64-65 0.3511349626213196 1104 66-67 0.3679060082394088 1104 68-69 -6.259365307066222 1104 70-71 -4.125281592163802 1104 72-73 0.37780837354553043 1104 74-75 0.9805336915710647 1104 76-77 1.1160147468243018 1104 78-79 0.6146054792842186 1104 80-81 0.3448200946048523 1104 82-83 0.3071600731660702 1104 84-85 0.31709430066636557 1104 86-87 0.397083250263087 1104 88-89 0.32925790286727974 1104 90-91 0.20610791632936554 1104 92-93 0.2974557711663586 1104 94-95 0.3427992902200536 1104 96-97 0.35465264442238364 1104 98-99 0.5845469184352972 1104 100-101 0.6091735386774921 1105 1 0.11089555158904929 1105 2 0.1962937091928154 1105 3 0.2294050500139555 1105 4 0.12497757364017303 1105 5 0.5265260278983064 1105 6 0.33411772613907686 1105 7 0.31480265610348823 1105 8 0.42870719275872915 1105 9 0.5118096197470337 1105 10-11 0.3987368904391033 1105 12-13 0.40475960189990445 1105 14-15 0.40490874565261237 1105 16-17 0.4674701789747786 1105 18-19 0.5850827046646145 1105 20-21 0.5115121808019083 1105 22-23 0.582603727181521 1105 24-25 0.5064503546884467 1105 26-27 0.6308635862156535 1105 28-29 0.5590955160729223 1105 30-31 0.49611121246731926 1105 32-33 0.6329929031501109 1105 34-35 0.6118568015853398 1105 36-37 0.7206556505423762 1105 38-39 0.756626284521154 1105 40-41 0.7872429197303958 1105 42-43 0.7164576660272672 1105 44-45 0.7001499916294378 1105 46-47 0.5726166791094514 1105 48-49 0.8036974744035561 1105 50-51 0.6175860741661481 1105 52-53 0.4022514286938659 1105 54-55 0.5074748827878821 1105 56-57 0.5785773302556692 1105 58-59 0.5466382331095012 1105 60-61 0.6395815934340376 1105 62-63 0.43823704352257664 1105 64-65 0.3977939588601451 1105 66-67 0.45446537210969495 1105 68-69 -3.4630198458047943 1105 70-71 -3.361432705415755 1105 72-73 0.7360971152376372 1105 74-75 1.0710370446794606 1105 76-77 1.0195679560497801 1105 78-79 0.7963677891977881 1105 80-81 0.47296355573150706 1105 82-83 0.543325537272711 1105 84-85 0.6057583109690263 1105 86-87 0.4246914869049405 1105 88-89 0.46590611724658615 1105 90-91 0.4000051217811915 1105 92-93 0.4518541209428619 1105 94-95 0.5052993214175636 1105 96-97 0.5321624217220773 1105 98-99 0.4538839463857691 1105 100-101 0.499566390204798 1106 1 -0.011332817420434793 1106 2 0.04541819565635663 1106 3 0.05682811992629766 1106 4 0.017155631054279752 1106 5 0.18653589391675496 1106 6 0.09560800479673759 1106 7 0.04972925369103365 1106 8 0.1780905666056185 1106 9 0.22564983358724788 1106 10-11 0.25568504030571404 1106 12-13 0.08393236047179897 1106 14-15 0.12442510679988317 1106 16-17 0.19226180076211108 1106 18-19 0.2168839158649689 1106 20-21 0.2199576328095887 1106 22-23 0.3602327215487193 1106 24-25 0.3455695639793319 1106 26-27 0.4574332582788898 1106 28-29 0.3782742783847297 1106 30-31 0.3683042091238278 1106 32-33 0.3922407020974745 1106 34-35 0.23881104441940693 1106 36-37 0.17684555737605478 1106 38-39 0.3311829191636235 1106 40-41 0.2310034278685862 1106 42-43 0.2697886581994595 1106 44-45 0.35020907301899484 1106 46-47 0.4723822655703245 1106 48-49 0.4828033123076807 1106 50-51 0.284241481164905 1106 52-53 0.059569490518363466 1106 54-55 -0.01755695232979093 1106 56-57 0.24731906873989828 1106 58-59 0.5202507764645325 1106 60-61 0.5791898334714674 1106 62-63 0.026193745213184627 1106 64-65 0.1522687208456368 1106 66-67 0.28762067522207957 1106 68-69 -3.4076149502711424 1106 70-71 -3.775575400674331 1106 72-73 0.26216877006465467 1106 74-75 0.9140255432473587 1106 76-77 1.104050519073116 1106 78-79 0.761758331841552 1106 80-81 0.502417253640143 1106 82-83 0.17768789802991947 1106 84-85 0.1972710494774752 1106 86-87 0.34714031171427706 1106 88-89 0.34483451978013946 1106 90-91 0.3553403560863835 1106 92-93 0.3659050392856251 1106 94-95 0.6350202558595264 1106 96-97 0.6696891740556943 1106 98-99 0.6770927350452034 1106 100-101 0.7204839426674141 1107 1 0.16679057623407445 1107 2 0.16395836435746958 1107 3 0.1389822408411483 1107 4 -0.028691017528416296 1107 5 0.25697782085010346 1107 6 0.1887872683086158 1107 7 0.1519683057691381 1107 8 0.11038174943328727 1107 9 0.3474030803404986 1107 10-11 0.06294777339999058 1107 12-13 0.18634433660963623 1107 14-15 0.12434991821879038 1107 16-17 -0.0016442276396304578 1107 18-19 0.218150509607419 1107 20-21 0.3170162206809479 1107 22-23 0.3432474565752486 1107 24-25 0.2628857361238275 1107 26-27 0.5858788693559305 1107 28-29 0.5341347879871918 1107 30-31 0.4026651971462982 1107 32-33 0.3465063041635048 1107 34-35 0.22405714816068212 1107 36-37 0.4545788219655478 1107 38-39 0.6777152837351466 1107 40-41 0.44482862419109637 1107 42-43 0.2946703692399737 1107 44-45 0.308209912189362 1107 46-47 0.4748278781956472 1107 48-49 0.4108415502976328 1107 50-51 0.4143734647035373 1107 52-53 0.2953915562089904 1107 54-55 0.2321633887263843 1107 56-57 0.5583036849820218 1107 58-59 0.6539491372954025 1107 60-61 0.6426597371371869 1107 62-63 0.4469312397167755 1107 64-65 0.35332618001736904 1107 66-67 0.4933114734557975 1107 68-69 -2.5553285787385285 1107 70-71 -2.81654862928168 1107 72-73 1.1969214354369555 1107 74-75 1.3008451494875644 1107 76-77 0.9613769447337681 1107 78-79 0.857156287486287 1107 80-81 0.7728162930842437 1107 82-83 0.8402522873244607 1107 84-85 0.5140747974105189 1107 86-87 0.5531759903894411 1107 88-89 0.7495500727655404 1107 90-91 0.6723758629019336 1107 92-93 0.6483989893627289 1107 94-95 0.6974794198476566 1107 96-97 0.587812196121849 1107 98-99 0.6417080404598643 1107 100-101 0.9104023635407685 1108 1 -0.026904369926683103 1108 2 0.06296036963501805 1108 3 0.09590122238878251 1108 4 0.06132875739783117 1108 5 -0.1241854703454166 1108 6 -0.17913650732892705 1108 7 0.07056225373671055 1108 8 0.21512019011168348 1108 9 0.1155541763366088 1108 10-11 0.23546048698666056 1108 12-13 0.1602238798413893 1108 14-15 0.23924985082050654 1108 16-17 0.24897875001494185 1108 18-19 0.186897086108047 1108 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2315 84-85 -0.19980664575871643 2315 86-87 0.32022983447816955 2315 88-89 0.4445005115386529 2315 90-91 0.45764864890146484 2315 92-93 -0.6166156713641406 2315 94-95 0.5506760455733541 2315 96-97 0.22644205786217597 2315 98-99 -2.14449179007136 2315 100-101 -3.6363630480502245 2316 1 -0.41061337338192416 2316 2 -0.38664840237779075 2316 3 -0.9564372503423257 2316 4 -0.3180114858602039 2316 5 -0.18746914548435 2316 6 -0.45898287654874537 2316 7 -0.5858282684954759 2316 8 -1.0959508844958847 2316 9 -5.0805767848897005 2316 10-11 -1.528642648125519 2316 12-13 -0.6126725886348154 2316 14-15 -0.48278816534655533 2316 16-17 -0.38375007304441056 2316 18-19 -0.371040543735603 2316 20-21 -3.7188948625212532 2316 22-23 -1.2408882580947491 2316 24-25 -0.4225757361219209 2316 26-27 -0.5997555625126907 2316 28-29 -0.10270149259492456 2316 30-31 -0.15328020057256708 2316 32-33 -1.219171277243106 2316 34-35 -0.6935604811954619 2316 36-37 -0.2400278640692406 2316 38-39 -5.617002659680363 2316 40-41 -5.5162588231854635 2316 42-43 -3.8123704114026715 2316 44-45 -2.3011249477280558 2316 46-47 -2.3551246514281488 2316 48-49 -2.615815247372815 2316 50-51 -1.1168008785963046 2316 52-53 -1.0744312137677383 2316 54-55 -0.38211311465631326 2316 56-57 -3.8857254882415546 2316 58-59 -0.7842160111934753 2316 60-61 -0.469771793549171 2316 62-63 -0.10389072829893564 2316 64-65 0.08556135634791673 2316 66-67 -0.1185889892269536 2316 68-69 2.4503362166857556 2316 70-71 3.602292326884509 2316 72-73 2.619397485888207 2316 74-75 1.2325843120855815 2316 76-77 -1.7367210878557735 2316 78-79 0.016004254506693627 2316 80-81 0.2389447884237903 2316 82-83 0.4820953601969933 2316 84-85 0.6127784198533206 2316 86-87 0.2859548441736237 2316 88-89 0.07680712228873787 2316 90-91 0.44515819689432234 2316 92-93 0.27512207070881445 2316 94-95 0.37977886234184055 2316 96-97 0.5403380370881052 2316 98-99 0.6944025118044692 2316 100-101 0.7382522673726939 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 32.0 4 92.0 5 229.0 6 577.0 7 1425.0 8 3048.0 9 5079.0 10 7817.0 11 11201.0 12 14892.0 13 19211.0 14 23367.0 15 28946.0 16 34366.0 17 38334.0 18 36784.0 19 33647.0 20 33927.0 21 34785.0 22 36541.0 23 41116.0 24 49119.0 25 60708.0 26 75686.0 27 94158.0 28 114638.0 29 136468.0 30 160120.0 31 185388.0 32 212634.0 33 245285.0 34 280614.0 35 308576.0 36 310785.0 37 244873.0 38 92926.0 39 4580.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.07318470457385 20.253351794533284 15.569761466806737 23.103702034086137 2 16.17987838929713 21.160389752609525 38.748326754388174 23.91140510370517 3 19.39785322693366 24.557573465004943 30.435981118484328 25.608592189577074 4 13.527029421952747 17.75547851958465 36.16114943516403 32.556342623298576 5 13.768598793889028 37.53082263099598 33.9920341544765 14.708544420638495 6 32.12005710297953 36.371224363937934 16.933675969095713 14.575042563986825 7 28.216846452073302 33.01003594590308 20.2889287356291 18.484188866394522 8 26.38444041058612 35.47524280000442 19.066888756165785 19.073428033243673 9 25.843357150833622 16.93407838614666 20.205192454277878 37.01737200874184 10-11 25.118544845980157 26.944379464244538 26.79117711422513 21.145898575550174 12-13 25.896945688118066 24.139053870564567 27.579786725669948 22.384213715647416 14-15 22.747730451669547 25.577393014911898 26.033331533634858 25.6415449997837 16-17 22.230650659119974 28.802014508201545 26.624291973573833 22.34304285910465 18-19 21.914743459581253 26.96972964072655 29.42368928796052 21.691837611731682 20-21 22.751150996602597 26.111635184798292 29.538384047383264 21.598829771215847 22-23 22.39925982015964 26.207369739146298 29.328737502422886 22.064632938271174 24-25 22.278089279567038 26.303166743268214 29.499522197485785 21.919221779678967 26-27 22.1868696802459 26.595865446157184 29.206643684440248 22.01062118915667 28-29 22.01681754411675 26.633518285293945 29.422268113216692 21.92739605737261 30-31 22.193395316862148 26.44755573068185 29.34721116038867 22.011837792067336 32-33 22.07295802996753 26.4934048433229 29.152275968733328 22.281361157976246 34-35 22.40497064379093 26.579144389958444 29.07808173124082 21.937803235009806 36-37 22.367113439603425 26.406361438019278 29.05442664326241 22.17209847911489 38-39 22.233038028262147 26.66259731288385 29.15912404054943 21.945240618304574 40-41 22.388756455765193 26.402162498854754 29.2113873170496 21.99769372833046 42-43 22.21946831136115 26.506988687411503 28.987740304826048 22.285802696401298 44-45 22.388468448875887 26.74394506809853 28.700237094270353 22.16734938875523 46-47 22.425142772377544 26.824342612761775 28.262545787591886 22.48796882726879 48-49 22.543787997363594 26.933544800812935 28.206913724758476 22.315753477064995 50-51 22.506480677772597 27.122213274998703 27.986913301292244 22.384392745936456 52-53 22.69615855721883 27.076037156668313 27.858307529159404 22.369496756953453 54-55 22.46331421178817 26.997831296035475 28.050276758029135 22.488577734147224 56-57 22.52474682928629 27.35159449749932 27.827545376175284 22.296113297039103 58-59 22.618482786380046 26.94931300654499 27.931992469696397 22.500211737378564 60-61 22.582271280657938 26.943330843592754 28.21547983628754 22.258918039461772 62-63 22.592334431675447 26.96213863658581 28.0619171851947 22.383609746544046 64-65 22.420598788578793 26.87504171156424 28.050142414599033 22.654217085257937 66-67 22.221417980468345 27.21980660467586 28.001983025050304 22.55679238980549 68-69 22.390515266882257 26.566079303710655 28.741240351529374 22.30216507787771 70-71 21.818550303230438 26.501946057818227 29.19798936963696 22.481514269314374 72-73 22.299371653389606 26.858048187824146 28.21649855786544 22.626081600920813 74-75 22.346346408817627 27.048497851845177 27.71149078706735 22.893664952269845 76-77 21.71828157729737 27.192163646248517 28.249578808082315 22.8399759683718 78-79 22.261640656928197 27.220934391867836 27.664479823956956 22.85294512724701 80-81 22.05989026411799 27.29147684000098 27.723552233870702 22.92508066201033 82-83 22.26772763109666 27.298796320919834 27.506748121441703 22.92672792654181 84-85 22.207139019804973 27.144331715391594 27.447570035727963 23.200959229075465 86-87 22.19561749761438 27.095738275239178 27.68466962549211 23.023974601654334 88-89 22.12958597224523 27.107949298015342 27.499937960559013 23.26252676918042 90-91 22.019382246439083 27.30059585442271 27.592997976928775 23.087023922209433 92-93 22.11505376848931 27.08614897154727 27.570108972325308 23.22868828763811 94-95 21.838096583729715 26.97671507386934 27.70126252956301 23.48392581283794 96-97 21.946983109640982 26.881393431555008 27.590756640110975 23.580866818693035 98-99 21.762470187696355 26.83729483550786 27.52817222866737 23.87206274812842 100-101 21.655304119059064 26.762245776482864 27.764214867357197 23.81823523710088 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2482.0 1 1730.0 2 961.0 3 982.5 4 938.0 5 977.5 6 1522.0 7 2750.5 8 4073.0 9 4807.0 10 4641.5 11 4034.5 12 3714.5 13 3675.5 14 3803.0 15 4003.5 16 4295.0 17 4550.5 18 4855.5 19 5318.0 20 5951.0 21 6815.0 22 7859.5 23 9141.0 24 10913.5 25 13444.0 26 16852.5 27 20702.5 28 24580.0 29 28732.0 30 33295.5 31 38352.0 32 43569.5 33 48461.5 34 53703.5 35 58847.5 36 65013.5 37 71517.0 38 76320.0 39 79064.5 40 81917.5 41 87698.5 42 93447.0 43 97921.5 44 102469.0 45 106540.0 46 108718.0 47 111458.5 48 117708.5 49 121038.0 50 119878.5 51 116720.0 52 111655.0 53 108664.0 54 105085.5 55 97127.0 56 87860.5 57 79184.5 58 70604.5 59 61733.5 60 52002.5 61 41460.0 62 31981.0 63 24359.5 64 18162.0 65 13573.5 66 10061.5 67 7470.0 68 5349.5 69 3682.5 70 2607.5 71 1906.5 72 1589.5 73 1318.0 74 995.5 75 771.5 76 523.0 77 296.0 78 164.5 79 93.0 80 65.0 81 38.0 82 23.5 83 19.0 84 12.0 85 7.5 86 4.5 87 2.5 88 1.0 89 0.5 90 0.5 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01988610926763115 2 0.016767377122791863 3 5.700908221749233E-4 4 0.0908456492512863 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.01017779791353466 12-13 0.0 14-15 0.0 16-17 4.1918442806979653E-4 18-19 1.5090639410512676E-4 20-21 0.0 22-23 1.0060426273675117E-4 24-25 0.003370242801681164 26-27 0.0037391250983825853 28-29 0.0011904837757182223 30-31 2.0120852547350233E-4 32-33 6.706950849116745E-4 34-35 0.002464804437050404 36-37 0.08269670396960947 38-39 0.09396438139612559 40-41 0.07645923967993089 42-43 0.07981271510448927 44-45 0.07929292641368271 46-47 0.07798507099810495 48-49 0.07273688195867109 50-51 0.07248537130182922 52-53 0.05638868926394903 54-55 0.04691512118957163 56-57 0.026525990608256726 58-59 0.02325635206931231 60-61 0.013816318749180495 62-63 0.002095922140348983 64-65 0.0065057423236432425 66-67 0.1579989946280677 68-69 0.7244345285902224 70-71 0.8849989319180773 72-73 0.3585200576395356 74-75 0.05474548630591543 76-77 0.19795565431168072 78-79 0.01467145498244288 80-81 0.0031522668990848703 82-83 0.001341390169823349 84-85 1.0060426273675117E-4 86-87 0.0018276441063843128 88-89 2.3474327971908607E-4 90-91 0.004309215920557509 92-93 0.004124774772206798 94-95 0.07806890788371891 96-97 0.03817931770859707 98-99 0.013749249240689328 100-101 0.013480971206724656 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2981981.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.343801314177995 #Duplication Level Percentage of deduplicated Percentage of total 1 73.93025891970122 40.17651301837979 2 14.50610177995133 15.766334259458379 3 5.205479247672224 8.486565899417283 4 2.2758199091853846 4.947068198864845 5 1.2014752900331591 3.2646367222728188 6 0.7264051069610549 2.3685368883777467 7 0.4485712152193267 1.7063945496596928 8 0.31920963090105303 1.3877651807407136 9 0.22002140977446608 1.0761119799884031 >10 1.0352854407440513 10.037182532029355 >50 0.07995574112112977 2.9854110437251764 >100 0.047480578851661334 4.907213901933413 >500 0.002998651340362006 1.1769649685645585 >1k 7.496628349601181E-4 0.6735262492340452 >5k 6.247190291334318E-5 0.253026419453669 >10k+ 1.2494380582668636E-4 0.7867481879000838 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11883 0.39849348470027146 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11291 0.37864091018688584 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7453 0.24993452339233552 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3159 0.10593628866179898 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3152 0.1057015453820799 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 3.353475424558372E-5 0.0 0.0 0.0 0.0 3 3.353475424558372E-5 0.0 0.0 3.353475424558372E-5 0.0 4 3.353475424558372E-5 0.0 0.0 3.353475424558372E-5 0.0 5 3.353475424558372E-5 0.0 0.0 3.353475424558372E-5 0.0 6 6.706950849116744E-5 0.0 0.0 3.353475424558372E-5 0.0 7 1.6767377122791862E-4 0.0 0.0 3.353475424558372E-5 0.0 8 1.6767377122791862E-4 0.0 0.0 3.353475424558372E-5 0.0 9 1.6767377122791862E-4 0.0 0.0 3.353475424558372E-5 0.0 10-11 1.6767377122791862E-4 0.0 0.0 5.030213136837558E-5 0.0 12-13 1.6767377122791862E-4 0.0 0.0 6.706950849116744E-5 0.0 14-15 2.0120852547350236E-4 0.0 0.0 1.0060426273675117E-4 0.0 16-17 2.1797590259629423E-4 0.0 0.0 1.341390169823349E-4 0.0 18-19 2.682780339646698E-4 0.0 0.0 1.6767377122791862E-4 0.0 20-21 2.682780339646698E-4 0.0 0.0 2.682780339646698E-4 0.0 22-23 3.018127882102535E-4 0.0 0.0 4.0241705094700466E-4 0.0 24-25 3.68882296701421E-4 0.0 0.0 8.886709875079687E-4 0.0 26-27 3.68882296701421E-4 1.676737712279186E-5 0.0 0.001341390169823349 0.0 28-29 3.68882296701421E-4 3.353475424558372E-5 0.0 0.002682780339646698 0.0 30-31 4.024170509470047E-4 3.353475424558372E-5 0.0 0.0058182798616087765 0.0 32-33 4.024170509470047E-4 3.353475424558372E-5 0.0 0.012441393825111561 0.0 34-35 4.024170509470047E-4 3.353475424558372E-5 0.0 0.02196526403085734 0.0 36-37 4.024170509470047E-4 3.353475424558372E-5 0.0 0.03841406098831615 0.0 38-39 4.359518051925884E-4 3.353475424558372E-5 0.0 0.062206969125557815 0.0 40-41 4.359518051925884E-4 3.353475424558372E-5 0.0 0.0875424759580963 0.0 42-43 4.359518051925884E-4 3.353475424558372E-5 0.0 0.1151583460793345 0.0 44-45 4.359518051925884E-4 3.353475424558372E-5 0.0 0.14840805491383077 0.0 46-47 5.030213136837558E-4 3.353475424558372E-5 0.0 0.19108102969133606 0.0 48-49 5.365560679293396E-4 3.353475424558372E-5 0.0 0.2385830090802054 0.0 50-51 5.365560679293396E-4 3.353475424558372E-5 0.0 0.2883150496264061 0.0 52-53 5.365560679293396E-4 3.353475424558372E-5 0.0 0.33905313279997423 0.0 54-55 5.365560679293396E-4 3.353475424558372E-5 0.0 0.3951903114070814 0.0 56-57 5.365560679293396E-4 3.353475424558372E-5 0.0 0.4625280979322135 0.0 58-59 5.365560679293396E-4 3.353475424558372E-5 0.0 0.5322636193859049 0.0 60-61 5.533234450521314E-4 6.706950849116744E-5 0.0 0.6042795041282959 0.0 62-63 5.700908221749233E-4 6.706950849116744E-5 0.0 0.6770834555954581 3.353475424558372E-5 64-65 5.700908221749233E-4 1.0060426273675118E-4 0.0 0.7550685265935632 3.353475424558372E-5 66-67 6.03625576420507E-4 1.0060426273675118E-4 0.0 0.8424265614033087 3.353475424558372E-5 68-69 6.203929535432989E-4 1.0060426273675118E-4 3.353475424558372E-5 0.9423433616780255 3.353475424558372E-5 70-71 6.874624620344664E-4 1.0060426273675118E-4 3.353475424558372E-5 1.0667069977977728 3.353475424558372E-5 72-73 7.545319705256338E-4 1.0060426273675118E-4 3.353475424558372E-5 1.2040150490563153 3.353475424558372E-5 74-75 7.712993476484257E-4 1.1737163985954303E-4 3.353475424558372E-5 1.3539657026654428 3.353475424558372E-5 76-77 7.712993476484257E-4 1.341390169823349E-4 3.353475424558372E-5 1.5266193848988308 3.353475424558372E-5 78-79 7.880667247712176E-4 1.341390169823349E-4 3.353475424558372E-5 1.7266877287279832 3.353475424558372E-5 80-81 9.054383646307605E-4 1.341390169823349E-4 3.353475424558372E-5 1.9501800983976758 3.353475424558372E-5 82-83 9.054383646307605E-4 1.341390169823349E-4 3.353475424558372E-5 2.1789877266152935 3.353475424558372E-5 84-85 9.389731188763443E-4 1.341390169823349E-4 3.353475424558372E-5 2.4142172602709406 3.353475424558372E-5 86-87 9.72507873121928E-4 1.341390169823349E-4 3.353475424558372E-5 2.663665529726715 3.353475424558372E-5 88-89 9.72507873121928E-4 1.341390169823349E-4 3.353475424558372E-5 2.9350790632133474 3.353475424558372E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6955 0.0 52.20477 1 GTATCAA 11800 0.0 38.653362 1 TATCAAC 15295 0.0 29.753279 2 ATCAACG 15260 0.0 29.693146 3 TCAACGC 15615 0.0 29.023443 4 CAACGCA 15985 0.0 28.385294 5 AACGCAG 16415 0.0 27.786142 6 GTGGTAT 3355 0.0 26.171288 1 TGGTATC 3390 0.0 24.63648 2 ACGCAGA 19290 0.0 23.546553 7 CGCAGAG 19385 0.0 23.43116 8 GCAGAGT 20260 0.0 21.670334 9 GAGTACT 11830 0.0 20.81684 12-13 GTACATG 12460 0.0 20.759857 1 TACATGG 12245 0.0 20.422905 2 GTACTTT 12340 0.0 19.918085 14-15 AGTACTT 12155 0.0 19.519247 12-13 CAGAGTA 20050 0.0 19.329802 10-11 AGAGTAC 19165 0.0 19.28241 10-11 ACATGGG 12500 0.0 19.092611 3 >>END_MODULE