FastQCFastQC Report
Fri 27 May 2016
SRR939513_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939513_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3112334
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90540.29090708130939674No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73130.2349683549387694No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46980.15094780958598916No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65850.054.4915281
GTATCAA116500.042.8213581
TATCAAC148750.033.5865362
ATCAACG153950.032.1746983
TCAACGC156400.031.4654964
CAACGCA155150.031.4511765
AACGCAG160750.030.547746
ACGCAGA181500.026.5357697
CGCAGAG183350.026.2769458
GTACATG129850.025.5230141
TACATGG131350.024.6157382
GCAGAGT187000.023.7794199
GTGGTAT32800.022.1835611
GAGTACT103450.022.14191812-13
ACATGGG140100.021.6914923
CAGAGTA177300.021.39713710-11
TGGTATC32300.021.2564852
AGAGTAC163700.021.2472910-11
GTACTTT109700.020.9305414-15
AGTACTT107300.020.165212-13
Produced by FastQC (version 0.11.5)