FastQCFastQC Report
Fri 27 May 2016
SRR939513_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939513_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3112334
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126580.4067044218261922No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117230.3766626589562688No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75740.24335434436021328No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA76550.051.7465361
GTATCAA125500.037.113341
TATCAAC165900.028.040622
ATCAACG166900.027.6406613
TCAACGC167600.027.5252174
CAACGCA170100.027.120675
AACGCAG177350.026.0386966
GTGGTAT37600.025.9596981
TGGTATC36200.025.7798392
TAGATCG1252.3014421E-422.7345855
ACGCAGA205500.022.3105017
CGCAGAG207900.021.9618198
GTACATG141000.021.8767171
TACATGG141700.021.4294342
GAGTACT120900.020.19617312-13
GCAGAGT220000.020.0219369
GTACTTT122550.019.6923514-15
ACATGGG150100.019.5955163
AGAGTAC192450.019.50446910-11
AGTACTT123950.019.45082312-13
Produced by FastQC (version 0.11.5)