FastQCFastQC Report
Fri 27 May 2016
SRR939512_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939512_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3082456
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88140.2859408212152907No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68720.22293911089079618No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47700.15474673442216208No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65700.049.4337231
GTATCAA116950.043.756161
TATCAAC151500.033.2510342
ATCAACG155200.032.1563073
TCAACGC156750.031.7779874
CAACGCA157600.031.1232725
AACGCAG161300.030.0262646
ACGCAGA182400.025.569957
GTACATG126650.025.28771
GTGGTAT36300.025.2672621
CGCAGAG184650.024.9436288
TACATGG126800.024.6761192
TGGTATC34500.024.0529272
GCAGAGT189000.022.600649
ACATGGG138650.021.915823
AGAGTAC161900.021.17892810-11
GAGTACT102700.021.16462712-13
AGTACTT106550.020.49004712-13
GTACTTT109250.020.15439814-15
CAGAGTA178200.020.15213210-11
Produced by FastQC (version 0.11.5)