##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939512_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3082456 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.14555374026426 31.0 27.0 31.0 10.0 33.0 2 27.183291180798687 31.0 26.0 31.0 16.0 34.0 3 27.45897459687989 31.0 26.0 31.0 16.0 34.0 4 31.275841406981964 35.0 30.0 37.0 19.0 37.0 5 31.65085633014713 35.0 32.0 37.0 19.0 37.0 6 31.466596441279293 35.0 30.0 37.0 17.0 37.0 7 31.425747845224716 35.0 30.0 37.0 17.0 37.0 8 31.344273202926498 35.0 30.0 37.0 17.0 37.0 9 32.43719845473869 35.0 31.0 39.0 17.0 39.0 10-11 31.557204709491394 35.0 29.5 38.5 14.0 39.0 12-13 31.289161142932777 35.0 29.0 38.0 15.5 39.0 14-15 32.098573507618596 36.0 30.0 39.0 17.0 40.0 16-17 31.967035376985105 36.0 29.5 39.0 16.5 40.0 18-19 31.85756552567174 36.0 29.0 39.0 16.0 40.0 20-21 31.705087436771198 36.0 28.0 39.0 14.0 40.0 22-23 31.89024498646534 36.0 29.5 39.0 15.5 40.0 24-25 31.966518419078813 36.0 29.5 39.0 15.5 41.0 26-27 32.059776522357495 36.0 30.0 39.0 15.5 41.0 28-29 31.657229981547182 36.0 29.0 39.0 10.5 40.5 30-31 31.36093329474938 36.0 29.0 39.0 10.0 40.0 32-33 31.21712167180975 35.0 29.0 39.0 9.5 40.0 34-35 30.77771296654356 35.0 27.0 39.0 9.0 40.0 36-37 30.417213579042166 35.0 26.5 38.5 9.0 40.0 38-39 30.029543649609273 35.0 25.0 38.0 8.0 40.0 40-41 29.856449045825798 35.0 25.5 38.0 8.0 40.0 42-43 29.577397536250317 34.0 25.0 38.0 7.0 40.0 44-45 29.2107403966188 34.0 24.5 38.0 2.0 40.0 46-47 28.62565678147555 34.0 24.0 38.0 2.0 40.0 48-49 28.276245305691305 33.0 23.0 38.0 2.0 40.0 50-51 27.588202881079244 33.0 22.5 37.5 2.0 39.5 52-53 27.67934416582102 33.0 23.0 38.0 2.0 39.5 54-55 27.78708406543354 33.0 23.0 38.0 2.0 40.0 56-57 27.308604891683775 33.0 21.5 38.0 2.0 40.0 58-59 26.885453677197663 33.0 20.0 37.5 2.0 40.0 60-61 26.257893381122067 32.0 18.0 37.0 2.0 39.0 62-63 25.650992585133416 31.0 14.5 36.0 2.0 39.0 64-65 25.10010215879805 31.0 13.0 36.0 2.0 39.0 66-67 24.70606474188115 31.0 10.0 35.5 2.0 38.5 68-69 23.989844623897305 30.0 7.5 35.0 2.0 38.0 70-71 23.37362690659656 29.5 6.5 34.5 2.0 37.5 72-73 22.88383581144386 29.0 2.0 34.0 2.0 37.0 74-75 22.14527539079228 29.0 2.0 34.0 2.0 36.0 76-77 21.36996586488177 27.5 2.0 34.0 2.0 35.5 78-79 20.86002200842445 27.0 2.0 33.5 2.0 35.0 80-81 20.312971215160896 26.0 2.0 33.0 2.0 35.0 82-83 19.575814869701304 26.0 2.0 33.0 2.0 35.0 84-85 18.863209077436952 25.0 2.0 32.0 2.0 34.5 86-87 18.07589792035961 23.5 2.0 32.0 2.0 34.0 88-89 17.283901862670547 20.0 2.0 31.5 2.0 34.0 90-91 16.48267469186908 17.5 2.0 31.0 2.0 34.0 92-93 15.641488799840127 9.0 2.0 31.0 2.0 34.0 94-95 14.569906269546102 2.0 2.0 30.5 2.0 34.0 96-97 13.482269819909838 2.0 2.0 29.5 2.0 34.0 98-99 12.452013751372283 2.0 2.0 29.0 2.0 34.0 100-101 11.120433511459693 2.0 2.0 27.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 3.8437787235875724 1101 2 3.7038131715906424 1101 3 3.3064442542949095 1101 4 3.01727991154171 1101 5 2.454780361467268 1101 6 2.7404713568810948 1101 7 2.548619355524515 1101 8 2.5667875708253938 1101 9 2.7058058623064056 1101 10-11 3.3886108402486563 1101 12-13 3.5162189740489147 1101 14-15 3.0072740972923633 1101 16-17 2.871686523641497 1101 18-19 2.8560077142421356 1101 20-21 2.8230148087746727 1101 22-23 3.021395854542863 1101 24-25 3.16866849507284 1101 26-27 2.9648894295737875 1101 28-29 3.1322234252886965 1101 30-31 3.1957607007636035 1101 32-33 2.9383838385234355 1101 34-35 3.1762999117740947 1101 36-37 3.2573570973506314 1101 38-39 3.439568972824123 1101 40-41 2.983392152572506 1101 42-43 2.8057484694116646 1101 44-45 2.866219238264442 1101 46-47 2.820518443484385 1101 48-49 2.788595157192546 1101 50-51 2.9277474197665576 1101 52-53 2.8056061091474582 1101 54-55 2.756761519362154 1101 56-57 3.101379638661353 1101 58-59 2.8726217008150883 1101 60-61 3.007392836906021 1101 62-63 2.824801792545511 1101 64-65 2.765557044618305 1101 66-67 2.57284673248056 1101 68-69 2.820766424213776 1101 70-71 2.518760838864722 1101 72-73 2.6504374295660575 1101 74-75 2.5706029788350406 1101 76-77 2.4169998851660637 1101 78-79 2.1409301873112447 1101 80-81 2.2176093911896047 1101 82-83 1.927580918394284 1101 84-85 1.8974326379565696 1101 86-87 2.0159064551922476 1101 88-89 1.915195613856902 1101 90-91 1.606124472491718 1101 92-93 1.2940006523836072 1101 94-95 1.5293974964355943 1101 96-97 1.3653634752798336 1101 98-99 1.0364001521840098 1101 100-101 0.889558239153855 1102 1 3.4568417878661997 1102 2 2.4335733512063165 1102 3 2.7588485298819094 1102 4 2.831068976466195 1102 5 1.814536799089769 1102 6 2.3389620113431064 1102 7 2.3442102801116604 1102 8 2.3760453158967145 1102 9 2.4225790318555767 1102 10-11 1.8848865001122697 1102 12-13 3.0380291272637443 1102 14-15 3.052646776424549 1102 16-17 2.9859040063055424 1102 18-19 3.0524406547561362 1102 20-21 2.9483063649131154 1102 22-23 2.969841915420414 1102 24-25 2.8814169980506854 1102 26-27 2.5621689960422955 1102 28-29 2.9225021363159733 1102 30-31 3.1312904761450646 1102 32-33 2.849658347004535 1102 34-35 2.9775844453787172 1102 36-37 3.128860560043588 1102 38-39 3.1170088243317657 1102 40-41 3.080728276209399 1102 42-43 2.8818215714070696 1102 44-45 2.7095033724832582 1102 46-47 2.6987443384369207 1102 48-49 2.6615342499570964 1102 50-51 2.83835369911894 1102 52-53 2.7761839007778057 1102 54-55 2.706520313720741 1102 56-57 2.9334189035036182 1102 58-59 2.96856649562163 1102 60-61 3.140809285664549 1102 62-63 3.0005674169411485 1102 64-65 2.829830499764313 1102 66-67 2.4772153071139833 1102 68-69 2.340269652132644 1102 70-71 2.4222957855728033 1102 72-73 2.3967664982865067 1102 74-75 2.4175629916801498 1102 76-77 2.40852631704065 1102 78-79 2.1206325729218456 1102 80-81 2.123814218540698 1102 82-83 2.053596023993151 1102 84-85 1.9055898007701515 1102 86-87 1.9063203637131103 1102 88-89 1.9243033418823607 1102 90-91 1.3839825322142865 1102 92-93 1.2589522988156272 1102 94-95 1.3196359540507743 1102 96-97 1.433740529665565 1102 98-99 1.2693537316793257 1102 100-101 0.6083446415062728 1103 1 3.686742627228689 1103 2 3.5215576348587234 1103 3 3.0456547312338422 1103 4 2.7697451882808686 1103 5 2.3302951788100295 1103 6 2.554569307461655 1103 7 2.3896563293125297 1103 8 2.2109482104543154 1103 9 2.5886486027738087 1103 10-11 3.2421324084175573 1103 12-13 2.533256478403576 1103 14-15 2.9230345177452897 1103 16-17 2.41921608777616 1103 18-19 2.3490337917743567 1103 20-21 2.609836391235035 1103 22-23 2.9561102852004346 1103 24-25 2.824310645390586 1103 26-27 2.75868229073302 1103 28-29 2.053379475939078 1103 30-31 2.685950656315047 1103 32-33 2.7226741255581857 1103 34-35 2.3418393886825086 1103 36-37 2.459160057102803 1103 38-39 2.8973073070805135 1103 40-41 2.8329101104105874 1103 42-43 2.7289052866039576 1103 44-45 2.73554457714274 1103 46-47 2.736317916180898 1103 48-49 2.453456356303395 1103 50-51 2.450568923971357 1103 52-53 2.3958764277201006 1103 54-55 2.5160419791301827 1103 56-57 2.6168454223998516 1103 58-59 2.5269841159085615 1103 60-61 2.697966728132002 1103 62-63 2.773408322382789 1103 64-65 2.832543274963399 1103 66-67 2.5936713602605543 1103 68-69 2.5863968014508956 1103 70-71 2.5137784297476777 1103 72-73 2.388478982966113 1103 74-75 2.3568586814445744 1103 76-77 2.2693177831190035 1103 78-79 1.9775396790761341 1103 80-81 1.8862429463880268 1103 82-83 2.1322553510943507 1103 84-85 2.1890536807255003 1103 86-87 1.9924583777561047 1103 88-89 1.888717550642724 1103 90-91 1.7727146209325433 1103 92-93 1.781587628603889 1103 94-95 1.81969340669127 1103 96-97 1.7666965844215632 1103 98-99 1.6194736785698804 1103 100-101 1.4047316975873105 1104 1 3.862512843077141 1104 2 3.6162699416276816 1104 3 2.8316082488116194 1104 4 2.696763632002938 1104 5 1.7489037663758857 1104 6 2.283802382799543 1104 7 2.4526299878016147 1104 8 2.3755314922860755 1104 9 2.4161669273146416 1104 10-11 3.4446793186902305 1104 12-13 3.417314451061518 1104 14-15 3.2600210792572817 1104 16-17 3.050813117143125 1104 18-19 3.090919696356945 1104 20-21 2.811787055115744 1104 22-23 2.465085052116631 1104 24-25 2.483995816409429 1104 26-27 2.4216139315037566 1104 28-29 2.312389263920455 1104 30-31 2.023652532060691 1104 32-33 2.454249359840219 1104 34-35 2.8856207096672577 1104 36-37 3.097858405945569 1104 38-39 3.1774508431746575 1104 40-41 3.2558785465463416 1104 42-43 3.137890395412686 1104 44-45 3.245870178172634 1104 46-47 3.0112270787574893 1104 48-49 2.7981586651535792 1104 50-51 2.860597445066361 1104 52-53 3.063656057412306 1104 54-55 2.872389349692668 1104 56-57 2.934606572007045 1104 58-59 3.008371733291497 1104 60-61 3.2045500835968284 1104 62-63 3.208472085668962 1104 64-65 3.1269833813064736 1104 66-67 3.1355515269071503 1104 68-69 3.1571011002082123 1104 70-71 3.1386575002897423 1104 72-73 2.963257254075348 1104 74-75 3.0774969485788333 1104 76-77 3.0867841736692334 1104 78-79 2.7697611546358907 1104 80-81 2.834620989128922 1104 82-83 2.7673739914257816 1104 84-85 2.522625225340825 1104 86-87 2.583157947063153 1104 88-89 2.517079450052247 1104 90-91 2.312115984111916 1104 92-93 2.4320181287802978 1104 94-95 2.497583445914108 1104 96-97 2.4329791344510365 1104 98-99 2.4109164126457276 1104 100-101 2.029456962717374 1105 1 3.3031489106062715 1105 2 3.1497757678502687 1105 3 2.8217357834478243 1105 4 2.6224944329938573 1105 5 -0.17116298286716614 1105 6 1.5457656363090422 1105 7 1.877299223511315 1105 8 1.8784542374920612 1105 9 2.361333915111686 1105 10-11 2.5146834475093804 1105 12-13 3.1257074228938 1105 14-15 2.835678937776411 1105 16-17 2.7474373487240022 1105 18-19 2.67940292876359 1105 20-21 2.6712133786316627 1105 22-23 2.429888703827796 1105 24-25 2.0885323564589733 1105 26-27 2.3169342590567368 1105 28-29 2.296809508897052 1105 30-31 2.5961141111046366 1105 32-33 2.5824597461141963 1105 34-35 2.767146996482559 1105 36-37 2.6094390555464564 1105 38-39 2.849901756102458 1105 40-41 2.7716746058178288 1105 42-43 2.8004871932840523 1105 44-45 2.6260143427748908 1105 46-47 2.661805572197256 1105 48-49 2.571909238600689 1105 50-51 2.8117834483694146 1105 52-53 2.784479871523697 1105 54-55 2.4886812113313503 1105 56-57 2.7200911348109713 1105 58-59 2.7518323718821946 1105 60-61 2.7699670943317614 1105 62-63 2.720076820048263 1105 64-65 2.658957759689816 1105 66-67 2.420118459653274 1105 68-69 2.5946126838397383 1105 70-71 2.4389038531661527 1105 72-73 2.413824393262427 1105 74-75 2.3753389524324007 1105 76-77 2.577393174494997 1105 78-79 2.4264898682793437 1105 80-81 2.546286508901378 1105 82-83 2.849975032805723 1105 84-85 2.7667093156243396 1105 86-87 2.7262942439711253 1105 88-89 2.7451199812936444 1105 90-91 2.6079039004345113 1105 92-93 2.565114513769748 1105 94-95 2.573573789064854 1105 96-97 2.559189107843091 1105 98-99 2.56901428859765 1105 100-101 2.3676206703969793 1106 1 3.113585600886445 1106 2 2.4789556772254677 1106 3 2.0767680126902768 1106 4 1.2986230946825437 1106 5 1.7700925216321686 1106 6 2.0787758794643167 1106 7 2.0315483778925767 1106 8 0.35748895572551476 1106 9 1.8338193959975158 1106 10-11 0.9416267750988929 1106 12-13 1.641597077626077 1106 14-15 1.7003994049408782 1106 16-17 1.8088495320158522 1106 18-19 1.7856961670046978 1106 20-21 1.8288805877707262 1106 22-23 2.3726900951099417 1106 24-25 2.624915900399234 1106 26-27 2.4633230681702756 1106 28-29 2.017255373342781 1106 30-31 2.111908274739843 1106 32-33 2.260897106507329 1106 34-35 2.323985020606628 1106 36-37 1.6882735376448643 1106 38-39 2.8212580966474725 1106 40-41 2.2757751387171865 1106 42-43 2.442253260040065 1106 44-45 2.5559442202399474 1106 46-47 2.8711166322521393 1106 48-49 2.745431320418291 1106 50-51 2.5351376030697814 1106 52-53 2.7301993654288594 1106 54-55 2.667176009978359 1106 56-57 2.848744287532906 1106 58-59 2.7851910737776535 1106 60-61 2.9170166826909423 1106 62-63 3.160013637968998 1106 64-65 3.1314894305048 1106 66-67 2.7537967416112785 1106 68-69 2.8482120679740177 1106 70-71 2.808347659407776 1106 72-73 2.7741748463864333 1106 74-75 2.9909602428785007 1106 76-77 3.100682950485808 1106 78-79 2.8015761054355117 1106 80-81 2.9074316043126984 1106 82-83 3.001973804265294 1106 84-85 2.883790973675268 1106 86-87 2.955404748789558 1106 88-89 3.0391830197772727 1106 90-91 2.9771796997835445 1106 92-93 2.9124473046306836 1106 94-95 3.0168196088363803 1106 96-97 2.8069434316672 1106 98-99 2.4609269026764036 1106 100-101 1.9810590678696958 1107 1 3.034871694830546 1107 2 3.180035280561434 1107 3 -0.05820735516832798 1107 4 0.5974840194264672 1107 5 1.3038670181246026 1107 6 1.8796605584552282 1107 7 1.9211694340967895 1107 8 1.7181148874309038 1107 9 2.056691612886823 1107 10-11 1.5719010414794319 1107 12-13 2.1486660330957896 1107 14-15 2.332890581136521 1107 16-17 0.7767645165642669 1107 18-19 0.07724033661177643 1107 20-21 -1.4222859932484013 1107 22-23 0.1877932626561858 1107 24-25 0.9514358821953586 1107 26-27 0.9329080515788064 1107 28-29 1.8499845312118417 1107 30-31 2.641613259836827 1107 32-33 1.2549409851634152 1107 34-35 0.8013793678006209 1107 36-37 1.1633220764727632 1107 38-39 2.007438616825123 1107 40-41 2.352413679836804 1107 42-43 1.522865866959215 1107 44-45 1.1678420478229903 1107 46-47 2.0237545622465127 1107 48-49 1.5661079724431275 1107 50-51 1.7832605633153662 1107 52-53 1.3329543252246445 1107 54-55 1.8996628966763218 1107 56-57 2.3606475581638087 1107 58-59 2.3661220178208993 1107 60-61 2.196064485981072 1107 62-63 2.195167566849811 1107 64-65 1.5101784198769295 1107 66-67 2.11606725134887 1107 68-69 2.19972768764152 1107 70-71 0.8489460033198775 1107 72-73 1.672748861839903 1107 74-75 2.0164120457577965 1107 76-77 2.1284189108829743 1107 78-79 2.0115836317095415 1107 80-81 2.025341436336994 1107 82-83 1.9695093562544344 1107 84-85 2.0179110908896334 1107 86-87 2.322986118082664 1107 88-89 2.192007440383204 1107 90-91 2.152684967577226 1107 92-93 2.3666784497676314 1107 94-95 2.2615583092142817 1107 96-97 2.022322186622553 1107 98-99 1.9254542948788487 1107 100-101 1.465707921215186 1108 1 2.381404284508786 1108 2 2.341431838089445 1108 3 1.7788051143591161 1108 4 1.7141141726244946 1108 5 1.9404257243686835 1108 6 -0.061738905017026724 1108 7 0.6149347064056521 1108 8 -0.385225750397737 1108 9 1.4370605272824406 1108 10-11 2.8716089697618763 1108 12-13 1.7783943741502277 1108 14-15 1.922353173319859 1108 16-17 0.18235182041640385 1108 18-19 0.5241308276433934 1108 20-21 1.0306328457827334 1108 22-23 -0.7907637333505271 1108 24-25 1.0264770279697224 1108 26-27 1.3908939169435115 1108 28-29 1.8464882100778048 1108 30-31 0.6182988673868017 1108 32-33 0.9887858133258192 1108 34-35 1.2799429395646094 1108 36-37 0.06937017876776608 1108 38-39 1.6817599153516234 1108 40-41 0.29407356131283535 1108 42-43 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2312 40-41 0.4194276223016047 2312 42-43 -1.7766817060556441 2312 44-45 -2.6080420559710085 2312 46-47 -0.421496646224675 2312 48-49 0.7504391184163737 2312 50-51 -0.6909996141929291 2312 52-53 -0.6857556873571191 2312 54-55 -0.6196882247999511 2312 56-57 0.10204133907680202 2312 58-59 0.27112373571289083 2312 60-61 0.29749452770784757 2312 62-63 -0.06584878918645032 2312 64-65 -1.5133000309139568 2312 66-67 1.3624368768306034 2312 68-69 0.4718751060524582 2312 70-71 -2.0624435887605976 2312 72-73 -2.620144598709448 2312 74-75 -0.49689134541568336 2312 76-77 0.04543614157494957 2312 78-79 0.0563708121774944 2312 80-81 -1.1405541399989119 2312 82-83 0.47137455064335754 2312 84-85 1.8836228826757875 2312 86-87 2.1270788860308834 2312 88-89 2.539000378899459 2312 90-91 2.972051328110158 2312 92-93 3.2326477663468207 2312 94-95 2.845153820892323 2312 96-97 2.3576042662678685 2312 98-99 1.457091873985517 2312 100-101 2.1950912774940274 2313 1 -7.791379329034793 2313 2 -4.31973597667335 2313 3 -1.8247144672779605 2313 4 0.21237566814825115 2313 5 0.7289775555334721 2313 6 -2.779719320143851 2313 7 -0.9442024919809029 2313 8 -2.150684057572601 2313 9 0.5191262085928159 2313 10-11 -1.4416308834486493 2313 12-13 -2.5112746304184235 2313 14-15 -3.6223693599274647 2313 16-17 -3.528250827575814 2313 18-19 -0.8836852871245107 2313 20-21 -2.754166009069582 2313 22-23 -1.5370141729206885 2313 24-25 -2.648180826438889 2313 26-27 -2.1740089317587774 2313 28-29 -2.7526847508020715 2313 30-31 -4.7460530174253215 2313 32-33 -5.810391929789738 2313 34-35 -4.539016899004373 2313 36-37 -1.8777323617140382 2313 38-39 -1.467917589371286 2313 40-41 -3.308596903815321 2313 42-43 -2.3757644562276923 2313 44-45 -2.8295092122528196 2313 46-47 -1.6292224035219327 2313 48-49 -2.678006966202691 2313 50-51 -3.0820180163970115 2313 52-53 -0.37953998859770977 2313 54-55 -1.7592377646517612 2313 56-57 -2.172778685722154 2313 58-59 -1.1473501300972195 2313 60-61 -1.6403733648923087 2313 62-63 -3.9426389360879206 2313 64-65 -5.202615544612112 2313 66-67 -3.8587004899376467 2313 68-69 -2.510019475041233 2313 70-71 -1.3219789655195164 2313 72-73 -1.5914326006673711 2313 74-75 -2.5519452914314193 2313 76-77 -2.5363894858375424 2313 78-79 -2.7953823275506906 2313 80-81 -3.390799133748395 2313 82-83 -1.2360189629715244 2313 84-85 -1.1124138039322737 2313 86-87 -2.5170991315897098 2313 88-89 -3.363398427257424 2313 90-91 -3.0380363212598667 2313 92-93 -1.406920983722996 2313 94-95 -0.6405410829330318 2313 96-97 -0.4857270969602876 2313 98-99 -0.3117778069420698 2313 100-101 0.03772261022278833 2314 1 -7.832983568026204 2314 2 -3.713858959994983 2314 3 -25.0004864387744 2314 4 -13.367465081176185 2314 5 -6.729742604999419 2314 6 -5.042712771248315 2314 7 -4.161697627590801 2314 8 -3.3210216959312397 2314 9 -3.349406075891462 2314 10-11 -14.392367834541897 2314 12-13 -13.093467116017582 2314 14-15 -5.112101089214139 2314 16-17 -3.2994711083160304 2314 18-19 -3.146491695617879 2314 20-21 -4.3703914346836825 2314 22-23 -4.4736043236126015 2314 24-25 -4.913162987670582 2314 26-27 -3.9370519082846904 2314 28-29 -3.298742110536139 2314 30-31 -3.573419739547635 2314 32-33 -3.898514163017609 2314 34-35 -2.948095918497195 2314 36-37 -3.483047449176894 2314 38-39 -0.6104772362502224 2314 40-41 -2.0620034193733545 2314 42-43 -4.141133364520716 2314 44-45 -4.8578706099916396 2314 46-47 -4.270621328387541 2314 48-49 -2.821018111826664 2314 50-51 -2.4487749187021066 2314 52-53 -0.8789811046346507 2314 54-55 -3.1256935075170524 2314 56-57 -2.8002018251530423 2314 58-59 -0.5471858467412858 2314 60-61 -3.268521434683084 2314 62-63 -4.452149900599512 2314 64-65 -3.7570943095859377 2314 66-67 -4.700918427436246 2314 68-69 -3.96358963284942 2314 70-71 -4.417526646159086 2314 72-73 -3.784828890588223 2314 74-75 -3.257751465921178 2314 76-77 -3.220855813359254 2314 78-79 -4.332548030955923 2314 80-81 -6.6437595999605215 2314 82-83 -6.2062299829387655 2314 84-85 -4.614974220138144 2314 86-87 -3.9012912261143455 2314 88-89 -3.039206941783279 2314 90-91 -4.8867755867449745 2314 92-93 -4.855689579797504 2314 94-95 -5.255460736351248 2314 96-97 -4.613623532732708 2314 98-99 -5.451441739292589 2314 100-101 -4.4629511919512 2315 1 -24.509906644949282 2315 2 -24.614668676594174 2315 3 -13.176560575252916 2315 4 -28.837505567006144 2315 5 -15.314453491151355 2315 6 -11.965919985707249 2315 7 -9.09957899421779 2315 8 -8.357625047353018 2315 9 -5.790250377689961 2315 10-11 -29.176983219157282 2315 12-13 -28.87707035488398 2315 14-15 -23.522634738157315 2315 16-17 -13.93716692910058 2315 18-19 -8.796370613312611 2315 20-21 -6.625808759344565 2315 22-23 -3.5502634070025003 2315 24-25 -5.132902984737321 2315 26-27 -5.419833151782914 2315 28-29 -17.344240406856944 2315 30-31 -16.21598443331173 2315 32-33 -9.339070254821873 2315 34-35 -7.403084680012803 2315 36-37 -5.078354401347678 2315 38-39 -5.273960688903632 2315 40-41 -8.574084193208662 2315 42-43 -7.8658211938731135 2315 44-45 -5.270807827963665 2315 46-47 -3.8381134580377 2315 48-49 -4.634711326783567 2315 50-51 -3.2721673379265646 2315 52-53 -7.09796408148576 2315 54-55 -6.672570777810275 2315 56-57 -7.664953913396463 2315 58-59 -7.334062741849905 2315 60-61 -4.903852057591855 2315 62-63 -3.5524505599011924 2315 64-65 -4.472424693740869 2315 66-67 -6.787469764628295 2315 68-69 -6.492390849811855 2315 70-71 -7.264694855061874 2315 72-73 -6.650710966655197 2315 74-75 -7.164683513167152 2315 76-77 -7.029108510424969 2315 78-79 -6.658824767122695 2315 80-81 -7.759341357802731 2315 82-83 -6.8780338364257645 2315 84-85 -6.194924354692988 2315 86-87 -6.532993643326451 2315 88-89 -6.0002496480239635 2315 90-91 -5.805086130455686 2315 92-93 -5.685938327192529 2315 94-95 -5.587567159170659 2315 96-97 -5.470513456979527 2315 98-99 -5.1873602082665276 2315 100-101 -5.02983194122897 2316 1 -1.3688629213949817 2316 2 1.6904559120282983 2316 3 2.037125220840082 2316 4 2.2212626511414832 2316 5 -1.3233973505211374 2316 6 -2.411116122036045 2316 7 -2.741401607079503 2316 8 -3.6984529870808913 2316 9 -3.27063991344183 2316 10-11 -2.9125262374929655 2316 12-13 -3.4325475528153397 2316 14-15 -1.7468010810294174 2316 16-17 -2.0630770690788474 2316 18-19 -4.205383350816415 2316 20-21 -1.6965365821895304 2316 22-23 -2.2996215610209774 2316 24-25 -2.489614681622829 2316 26-27 -2.270466422654593 2316 28-29 -1.5335624939238137 2316 30-31 1.1965621208209818 2316 32-33 -1.2921578686797233 2316 34-35 -6.557072561580441 2316 36-37 -2.56081364779158 2316 38-39 -0.3638668538332901 2316 40-41 -0.6290366855158176 2316 42-43 -0.9188291364448311 2316 44-45 -0.0793623615034349 2316 46-47 0.8338091766479643 2316 48-49 1.2370412711974588 2316 50-51 1.6377631145659386 2316 52-53 1.306178468922223 2316 54-55 -0.34799564182646137 2316 56-57 0.7276442221013646 2316 58-59 -2.998100632349118 2316 60-61 -3.0704372310090946 2316 62-63 -4.328837143280371 2316 64-65 -4.670913929700561 2316 66-67 -2.2232611046783326 2316 68-69 -1.809628226675855 2316 70-71 -3.021183123796707 2316 72-73 -2.8849953450231034 2316 74-75 -2.168450422273761 2316 76-77 -1.920499397202228 2316 78-79 -2.4384864285393384 2316 80-81 -2.443708397853051 2316 82-83 -3.137994133469153 2316 84-85 -2.377457351042331 2316 86-87 -1.7246628944084392 2316 88-89 -3.2117811705634303 2316 90-91 -3.4833525640694845 2316 92-93 -3.992318882249686 2316 94-95 -5.1384276605511925 2316 96-97 -4.893081304318015 2316 98-99 -4.991977914818744 2316 100-101 -4.7934283422969575 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 41891.0 3 16604.0 4 15668.0 5 16274.0 6 19231.0 7 23411.0 8 28461.0 9 33771.0 10 39664.0 11 44529.0 12 49795.0 13 52758.0 14 57654.0 15 61679.0 16 65605.0 17 67078.0 18 65285.0 19 67870.0 20 72378.0 21 78197.0 22 84603.0 23 91691.0 24 100039.0 25 108164.0 26 116564.0 27 125627.0 28 135374.0 29 144873.0 30 153724.0 31 163067.0 32 171057.0 33 178452.0 34 180753.0 35 172872.0 36 143768.0 37 80291.0 38 13647.0 39 87.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.51284754962098 17.95632204884874 14.471616428911801 23.05921397261848 2 16.40200691912804 19.49160085877964 39.28916853550437 24.817223686587944 3 20.047583881737843 23.53833482612889 28.949699612418968 27.4643816797143 4 13.688547255232084 16.058025364008373 35.131916731704685 35.12151064905486 5 14.982570358691879 36.33206131178522 32.846393000068886 15.838975329454014 6 33.627954792382795 34.15694210201263 16.880348402322596 15.33475470328199 7 29.97297032255718 32.175772135013304 19.408698204754028 18.442559337675487 8 27.718131680959107 33.70653773973677 18.766356851911446 19.808973727392676 9 27.406115482632405 16.46706897940204 19.308543387168424 36.818272150797135 10-11 26.228529713812005 25.687218255981325 25.854616993108227 22.229635037098443 12-13 27.209902923182312 23.029860171195267 26.62072802601598 23.139508879606442 14-15 24.19813719673211 24.34738314283665 25.01556682814874 26.4389128322825 16-17 23.387598225711084 27.459315942202156 25.603089062848543 23.549996769238213 18-19 23.360394628829127 25.78886474396276 28.223407170913173 22.62733345629494 20-21 24.34513131478488 24.705846241993903 28.399719106506637 22.54930333671458 22-23 24.07353926258676 24.716009685947384 28.299397649462005 22.91105340200385 24-25 23.62278951452696 24.878606966305604 28.469706202135832 23.028897317031607 26-27 23.283956433102396 25.183031326906054 28.4107828883781 23.122229351613456 28-29 23.248716859054912 25.4570043008908 28.462947664287213 22.831331175767072 30-31 23.38825780595462 25.192244320416084 28.266707268008506 23.152790605620794 32-33 23.244963177296484 25.202065905572063 28.28019372332527 23.27277719380618 34-35 23.707107441023645 25.321529723874935 28.100128715849852 22.871234119251564 36-37 23.738629543817417 25.111099741670056 28.09686114095589 23.05340957355663 38-39 23.44533644086372 25.336593938687287 28.072727545123733 23.14534207532526 40-41 23.656656486776832 25.268218849496517 28.014158194026372 23.06096646970028 42-43 23.498533893412304 25.259502084055697 27.86300855952896 23.378955463003038 44-45 23.441880739852785 25.418939112552803 27.840576982137726 23.29860316545669 46-47 23.743851902168394 25.632092108295858 27.437185488889206 23.186870500646545 48-49 23.714354593513463 25.775208091619774 27.139146761815308 23.371290553051463 50-51 23.694287323240676 26.054349440228968 27.025000495545132 23.22636274098522 52-53 23.74963944368293 26.0108536592302 26.811024613707072 23.428482283379797 54-55 23.571226954795137 26.004979988881722 26.843693576795847 23.5800994795273 56-57 23.39430285442108 26.175863971340775 26.953264353819034 23.476568820419114 58-59 23.499057365486372 25.947893028727652 26.89855134609053 23.65449825969544 60-61 23.45598538743736 25.94959166052424 27.164127769633883 23.430295182404514 62-63 23.508783212313254 25.95218050504932 27.09349141946221 23.445544863175208 64-65 23.326299598144963 26.09988856666724 27.00710724459568 23.566704590592117 66-67 23.32349112211763 26.185510660717497 26.822761765713153 23.668236451451723 68-69 23.306526491228695 25.895454939596107 27.14771649288173 23.650302076293467 70-71 23.304156305460065 25.96211436173668 26.784985799625304 23.948743533177947 72-73 23.345817869784753 26.262031031969652 26.46509492205462 23.927056176190977 74-75 23.221723270374866 26.4326473485449 26.498420406964506 23.847208974115727 76-77 23.41129293398339 26.134482057734417 26.53821776251879 23.916007245763407 78-79 23.307952560816123 26.230714000564447 26.487413843723917 23.97391959489551 80-81 23.264482206260276 26.206083502294238 26.462404730504453 24.067029560941037 82-83 23.344168588072296 26.267461613044933 26.386888778557893 24.001481020324874 84-85 23.22772706014754 26.311704442136662 26.314303541321372 24.146264956394425 86-87 23.267968292881818 26.210678394449467 26.47418964037911 24.047163672289603 88-89 23.32079860564014 26.14470219598683 26.2071105205621 24.327388677810934 90-91 23.05437022279491 26.291790696117936 26.309465095984336 24.34437398510282 92-93 23.104760459875713 26.141295528737036 26.286056417437642 24.467887593949605 94-95 23.06516956626808 26.06761523906537 26.31697668103236 24.55023851363419 96-97 23.106378933049683 25.83696629934773 26.286801302423395 24.76985346517919 98-99 22.950466071548448 25.974993851759876 26.052657543268896 25.021882533422772 100-101 22.993149741706908 25.939559107601557 26.174756782744236 24.8925343679473 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3155.0 1 2020.5 2 885.0 3 887.0 4 856.0 5 872.0 6 1149.0 7 1979.5 8 3225.0 9 4008.0 10 3807.5 11 3337.0 12 3262.5 13 3363.5 14 3571.5 15 3907.5 16 4146.5 17 4509.0 18 4938.0 19 5341.5 20 5850.5 21 6523.5 22 7539.0 23 8822.0 24 10386.5 25 12289.0 26 14488.0 27 17171.0 28 19932.5 29 22665.0 30 26013.0 31 30040.0 32 34436.0 33 38164.0 34 41047.0 35 43896.5 36 48282.0 37 53674.5 38 58803.0 39 63899.5 40 68759.0 41 73912.0 42 79000.0 43 84516.5 44 91564.5 45 98833.5 46 105351.0 47 111076.5 48 116106.0 49 119640.5 50 121195.0 51 122569.5 52 124891.5 53 127402.5 54 127625.0 55 123567.5 56 117015.0 57 108902.5 58 99970.5 59 89793.0 60 76716.0 61 63333.5 62 50863.5 63 39473.5 64 30158.5 65 22719.5 66 16859.0 67 12580.0 68 9489.5 69 7210.5 70 5463.0 71 4018.5 72 2907.5 73 2141.0 74 1596.5 75 1128.0 76 815.5 77 549.0 78 349.5 79 259.5 80 175.5 81 114.5 82 79.0 83 54.0 84 35.0 85 29.5 86 27.0 87 17.5 88 10.0 89 9.5 90 6.0 91 2.5 92 1.5 93 1.0 94 0.5 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 4.007388913256182 2 2.3251588992673375 3 0.9375965139486176 4 0.861358604956567 5 0.16026181720031038 6 0.0907717741956414 7 0.0933671072677112 8 0.1002122982452953 9 0.09498919043775483 10-11 1.6762120854279834 12-13 1.2832786583166151 14-15 0.48779285089551966 16-17 0.08717074955814455 18-19 0.09421059051613388 20-21 0.12032612955383629 22-23 0.135671036342449 24-25 0.14730137267166182 26-27 0.14374901052926625 28-29 0.5303238716140636 30-31 0.17460103242349606 32-33 0.1580233424256502 34-35 0.14138076910100256 36-37 0.13069124101041507 38-39 0.3914086689315273 40-41 0.11917445050310532 42-43 0.12079653367314894 44-45 0.1238136083694301 46-47 0.1351681905597355 48-49 0.1493938599610181 50-51 0.16340865855019504 52-53 0.18211127750079806 54-55 0.17977547773593525 56-57 0.1503346681996434 58-59 0.133513665726291 60-61 0.11229681786212034 62-63 0.11769835481836562 64-65 0.11305919695204084 66-67 0.10937706815604181 68-69 0.10598691433065062 70-71 0.11886625470079702 72-73 0.1334812240628901 74-75 0.1443005188070811 76-77 0.16452789593752515 78-79 0.16631218742457315 80-81 0.16439812928392164 82-83 0.15727718416743014 84-85 0.14490068957999724 86-87 0.14303529393444706 88-89 0.14082926082318775 90-91 0.14786910178117707 92-93 0.13873677353383146 94-95 0.18808054356655862 96-97 0.19087052661903367 98-99 0.20673450002206034 100-101 0.23262294741595663 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3082456.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.02254075407528 #Duplication Level Percentage of deduplicated Percentage of total 1 77.17890801247059 44.00937427496131 2 11.532066364188092 13.151754484612324 3 4.453680819188065 7.618805880533847 4 2.245408290226838 5.121555429559935 5 1.2597998966254595 3.5918495473652547 6 0.8040666746171823 2.7509954833411236 7 0.5106054712370149 2.0381214905006546 8 0.3663984065705182 1.6714374456716494 9 0.2733018983480454 1.4025931773045806 >10 1.2873373113550226 12.630544526868151 >50 0.06510857189943528 2.5421748509968327 >100 0.022109929948637766 2.266948061911299 >500 7.854296615233141E-4 0.27516563501040747 >1k 3.0208833122678883E-4 0.388270527788127 >5k 1.2083533249071553E-4 0.5404091835744951 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8814 0.2859408212152907 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6872 0.22293911089079618 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4770 0.15474673442216208 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 3.244166340087255E-5 3.244166340087255E-5 2 0.0 0.0 0.0 3.244166340087255E-5 3.244166340087255E-5 3 0.0 0.0 0.0 3.244166340087255E-5 3.244166340087255E-5 4 0.0 0.0 0.0 3.244166340087255E-5 6.48833268017451E-5 5 0.0 3.244166340087255E-5 0.0 3.244166340087255E-5 6.48833268017451E-5 6 0.0 3.244166340087255E-5 0.0 3.244166340087255E-5 6.48833268017451E-5 7 0.0 3.244166340087255E-5 0.0 3.244166340087255E-5 6.48833268017451E-5 8 0.0 3.244166340087255E-5 0.0 6.48833268017451E-5 6.48833268017451E-5 9 0.0 3.244166340087255E-5 0.0 6.48833268017451E-5 9.732499020261765E-5 10-11 0.0 4.8662495101308823E-5 0.0 9.732499020261765E-5 1.297666536034902E-4 12-13 0.0 6.48833268017451E-5 0.0 9.732499020261765E-5 3.730791291100343E-4 14-15 0.0 6.48833268017451E-5 0.0 1.6220831700436276E-4 4.541832876122157E-4 16-17 0.0 9.732499020261765E-5 0.0 2.1087081210567157E-4 5.190666144139608E-4 18-19 0.0 9.732499020261765E-5 0.0 2.2709164380610786E-4 5.839499412157059E-4 20-21 0.0 9.732499020261765E-5 0.0 2.595333072069804E-4 5.839499412157059E-4 22-23 0.0 9.732499020261765E-5 0.0 3.568582974095981E-4 6.001707729161422E-4 24-25 0.0 9.732499020261765E-5 0.0 5.352874461143971E-4 6.48833268017451E-4 26-27 0.0 9.732499020261765E-5 0.0 0.001054354060528358 6.812749314183236E-4 28-29 0.0 9.732499020261765E-5 0.0 0.0021411497844575885 6.974957631187598E-4 30-31 0.0 9.732499020261765E-5 0.0 0.005077120322236554 7.137165948191962E-4 32-33 0.0 9.732499020261765E-5 0.0 0.011727661319415427 7.785999216209412E-4 34-35 0.0 9.732499020261765E-5 0.0 0.021265510359271957 8.110415850218137E-4 36-37 0.0 9.732499020261765E-5 0.0 0.03829738364473005 8.110415850218137E-4 38-39 0.0 9.732499020261765E-5 0.0 0.062482643710080533 8.110415850218137E-4 40-41 0.0 9.732499020261765E-5 0.0 0.08931189934260213 8.110415850218137E-4 42-43 0.0 9.732499020261765E-5 0.0 0.11685487156994293 8.434832484226863E-4 44-45 0.0 9.732499020261765E-5 1.6220831700436274E-5 0.15114570978466524 9.083665752244314E-4 46-47 0.0 9.732499020261765E-5 3.244166340087255E-5 0.19439044709802833 9.732499020261765E-4 48-49 0.0 9.732499020261765E-5 3.244166340087255E-5 0.24220945895091445 0.0010381332288279216 50-51 0.0 9.732499020261765E-5 3.244166340087255E-5 0.29270490803437255 0.0010381332288279216 52-53 1.6220831700436274E-5 9.732499020261765E-5 3.244166340087255E-5 0.3441087236930551 0.001054354060528358 54-55 3.244166340087255E-5 9.732499020261765E-5 3.244166340087255E-5 0.4014007012589961 0.0011030165556296667 56-57 3.244166340087255E-5 9.732499020261765E-5 3.244166340087255E-5 0.4708420817685638 0.001119237387330103 58-59 3.244166340087255E-5 9.732499020261765E-5 3.244166340087255E-5 0.5486696322672571 0.0011354582190305394 60-61 3.244166340087255E-5 9.732499020261765E-5 3.244166340087255E-5 0.6361647984594103 0.0011354582190305394 62-63 3.244166340087255E-5 9.732499020261765E-5 3.244166340087255E-5 0.7278287183985757 0.001232783209233157 64-65 3.244166340087255E-5 9.732499020261765E-5 3.244166340087255E-5 0.8231423254703392 0.001232783209233157 66-67 3.244166340087255E-5 9.732499020261765E-5 3.244166340087255E-5 0.9320814311704693 0.0012652248726340294 68-69 3.244166340087255E-5 9.732499020261765E-5 6.48833268017451E-5 1.0561545728471065 0.001297666536034902 70-71 6.48833268017451E-5 9.732499020261765E-5 6.48833268017451E-5 1.193674784003405 0.0013463290311362109 72-73 6.48833268017451E-5 9.732499020261765E-5 6.48833268017451E-5 1.334731136470399 0.0014274331896383923 74-75 8.110415850218138E-5 9.732499020261765E-5 6.48833268017451E-5 1.4802644384867132 0.0014598748530392648 76-77 9.732499020261765E-5 9.732499020261765E-5 6.48833268017451E-5 1.6425700804812786 0.001476095684739701 78-79 9.732499020261765E-5 9.732499020261765E-5 6.48833268017451E-5 1.832791773832295 0.0015085373481405736 80-81 9.732499020261765E-5 9.732499020261765E-5 6.48833268017451E-5 2.035763040899854 0.0015409790115414462 82-83 9.732499020261765E-5 9.732499020261765E-5 6.48833268017451E-5 2.2511432442182464 0.0016220831700436275 84-85 9.732499020261765E-5 9.732499020261765E-5 6.48833268017451E-5 2.4675291391020666 0.0016220831700436275 86-87 9.732499020261765E-5 9.732499020261765E-5 6.48833268017451E-5 2.6956102536419015 0.0016707456651449365 88-89 9.732499020261765E-5 9.732499020261765E-5 6.48833268017451E-5 2.9434645620245674 0.0017194081602462453 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6570 0.0 49.433723 1 GTATCAA 11695 0.0 43.75616 1 TATCAAC 15150 0.0 33.251034 2 ATCAACG 15520 0.0 32.156307 3 TCAACGC 15675 0.0 31.777987 4 CAACGCA 15760 0.0 31.123272 5 AACGCAG 16130 0.0 30.026264 6 ACGCAGA 18240 0.0 25.56995 7 GTACATG 12665 0.0 25.2877 1 GTGGTAT 3630 0.0 25.267262 1 CGCAGAG 18465 0.0 24.943628 8 TACATGG 12680 0.0 24.676119 2 TGGTATC 3450 0.0 24.052927 2 GCAGAGT 18900 0.0 22.60064 9 ACATGGG 13865 0.0 21.91582 3 AGAGTAC 16190 0.0 21.178928 10-11 GAGTACT 10270 0.0 21.164627 12-13 AGTACTT 10655 0.0 20.490047 12-13 GTACTTT 10925 0.0 20.154398 14-15 CAGAGTA 17820 0.0 20.152132 10-11 >>END_MODULE