FastQCFastQC Report
Fri 27 May 2016
SRR939512_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939512_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3082456
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124290.40321743440944496No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111670.36227605519754374No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75900.24623222521262264No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68300.051.1469841
GTATCAA119450.041.761831
TATCAAC157750.031.5230262
ATCAACG161400.030.5415843
TCAACGC162350.030.3118064
CAACGCA165350.029.6779425
AACGCAG169800.028.9280836
TGGTATC32500.027.3019852
GTGGTAT34400.026.6294231
ACGCAGA194750.025.0516037
CGCAGAG197650.024.612078
GCAGAGT209450.022.2973639
GTATAGG6350.021.6764011
AGAGTAC186950.021.0603710-11
GTACATG139750.021.0573041
GAGTACT116100.020.90435212-13
AGTACTT118300.020.7760512-13
TACATGG138400.020.7422222
GTACTTT119750.020.08905614-15
CAGAGTA199550.019.48112110-11
Produced by FastQC (version 0.11.5)