##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939512_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3082456 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.872811160970343 31.0 31.0 31.0 28.0 34.0 2 31.128019670029353 31.0 31.0 34.0 28.0 34.0 3 31.13625563511693 31.0 31.0 34.0 28.0 34.0 4 35.05621848292401 35.0 35.0 37.0 32.0 37.0 5 34.62103660198232 35.0 35.0 37.0 30.0 37.0 6 34.676688329046705 35.0 35.0 37.0 31.0 37.0 7 34.51022009722118 35.0 35.0 37.0 30.0 37.0 8 34.409263262800835 35.0 35.0 37.0 30.0 37.0 9 35.91769095811911 37.0 35.0 39.0 30.0 39.0 10-11 35.70224262730757 37.0 35.0 39.0 30.0 39.0 12-13 35.68349994290267 37.0 35.0 39.0 30.0 39.0 14-15 36.53010148401145 38.0 35.0 40.0 30.0 41.0 16-17 36.4320454209241 38.0 35.0 40.0 30.0 41.0 18-19 36.4081526224543 38.0 35.0 40.0 30.0 41.0 20-21 36.26297601652708 38.0 34.0 40.0 29.5 41.0 22-23 36.09486104586732 38.0 34.0 40.0 29.0 41.0 24-25 35.927415995556785 38.0 34.0 40.0 29.0 41.0 26-27 35.447438341374536 38.0 34.0 40.0 27.0 41.0 28-29 35.23157167531345 38.0 33.5 40.0 27.0 41.0 30-31 34.92479016732112 37.0 33.0 40.0 26.5 41.0 32-33 34.68417618937626 37.0 33.0 40.0 25.0 41.0 34-35 34.96214317414426 37.5 33.0 40.0 26.0 41.0 36-37 34.829254334855065 38.0 33.0 40.0 25.5 41.0 38-39 34.63566730555115 37.0 33.0 40.0 25.0 41.0 40-41 34.47062748016516 37.0 33.0 40.0 24.5 41.0 42-43 34.288490411541964 37.0 33.0 40.0 24.0 41.0 44-45 33.89314997521457 37.0 32.5 40.0 24.0 41.0 46-47 33.48467455820943 37.0 32.0 40.0 23.0 41.0 48-49 33.07820792900207 36.5 31.0 39.0 21.0 40.5 50-51 32.793317244431066 36.0 31.0 39.0 20.0 40.0 52-53 32.64925598289156 36.0 31.0 39.0 20.0 40.0 54-55 32.28121017785817 36.0 30.5 39.0 18.5 40.0 56-57 31.740430195921697 35.0 29.5 39.0 16.0 40.0 58-59 31.242838340595938 35.0 29.0 38.0 13.0 40.0 60-61 30.83819606832993 34.0 28.0 38.0 11.5 40.0 62-63 30.625045742745392 34.0 28.0 38.0 10.0 40.0 64-65 30.120025557542426 34.0 28.0 37.0 8.0 39.5 66-67 29.194546004874034 33.5 27.0 36.5 4.5 39.0 68-69 25.671066350987655 31.0 16.5 35.0 2.0 38.0 70-71 24.31685026485374 29.0 12.0 34.0 2.0 37.5 72-73 25.341008111713517 30.0 18.5 34.0 2.0 37.0 74-75 26.104344879537614 31.0 21.0 34.0 2.0 37.0 76-77 25.8216814773674 30.5 21.5 34.0 2.0 36.0 78-79 26.968302061732594 31.5 25.0 34.5 2.0 36.0 80-81 27.018791509108322 32.0 25.0 35.0 2.0 36.0 82-83 26.713039699512336 32.0 25.0 34.5 2.0 35.5 84-85 26.3669231612714 32.0 24.5 34.0 2.0 35.0 86-87 25.927909595465437 31.0 23.5 34.0 2.0 35.0 88-89 25.452901030866297 31.0 21.5 34.0 2.0 35.0 90-91 24.858269996392487 31.0 19.5 34.0 2.0 35.0 92-93 24.27992840773721 31.0 17.5 34.0 2.0 35.0 94-95 23.411100434199223 30.0 7.0 34.0 2.0 35.0 96-97 22.721188072108735 30.0 2.0 34.0 2.0 35.0 98-99 21.85363473152577 29.5 2.0 34.0 2.0 35.0 100-101 20.45188074055234 27.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.246351283912297 1101 2 0.26697342075456376 1101 3 0.2811411374413275 1101 4 0.3020666701278927 1101 5 0.5288933091881844 1101 6 0.399500005008818 1101 7 0.49923342494405176 1101 8 0.6069256627208119 1101 9 0.6359238161885159 1101 10-11 0.740347965365622 1101 12-13 0.7147333959218614 1101 14-15 0.7134034748836058 1101 16-17 0.6858043997839971 1101 18-19 0.5201477362070293 1101 20-21 0.6757074516191253 1101 22-23 0.7690488189301732 1101 24-25 0.8751544095425317 1101 26-27 0.8767303705534033 1101 28-29 0.7934131431223648 1101 30-31 0.548907694534698 1101 32-33 0.847277786223664 1101 34-35 0.7942735609302787 1101 36-37 0.8583102921576824 1101 38-39 0.7615224541183565 1101 40-41 0.9513825564049654 1101 42-43 0.665222023074044 1101 44-45 0.8380964589807576 1101 46-47 0.714601870594322 1101 48-49 0.8257965192177821 1101 50-51 0.6038836037236095 1101 52-53 0.38947881169920606 1101 54-55 0.4373013514728612 1101 56-57 0.5420518755060968 1101 58-59 0.6413662692471185 1101 60-61 0.47858046171651125 1101 62-63 0.007743772888289868 1101 64-65 0.04042015771008067 1101 66-67 -0.2823953890489683 1101 68-69 -6.85992204877725 1101 70-71 -7.790895692957708 1101 72-73 -5.006343793453052 1101 74-75 -3.0778532133970025 1101 76-77 -0.3556359198268595 1101 78-79 -0.037529038606699316 1101 80-81 -0.1631199669006307 1101 82-83 -0.3765782461588465 1101 84-85 -0.4454790560169606 1101 86-87 -0.4335293980921371 1101 88-89 -0.2513717899530583 1101 90-91 -0.5136008707166759 1101 92-93 -0.5100116028547212 1101 94-95 -0.6847759879029773 1101 96-97 -0.976414379510814 1101 98-99 -1.044874096549112 1101 100-101 -1.0885998155980197 1102 1 0.40690662808122013 1102 2 0.40290154750018914 1102 3 0.3457768732961881 1102 4 0.322607154266251 1102 5 0.4940201366706063 1102 6 0.4047556614002943 1102 7 0.5075233790416718 1102 8 0.5619162762547774 1102 9 0.45714312127519463 1102 10-11 0.5982155284915152 1102 12-13 0.5756851799800771 1102 14-15 0.787734998387549 1102 16-17 0.5365929843660808 1102 18-19 0.5054871736667153 1102 20-21 0.6342908508421132 1102 22-23 0.669860813457511 1102 24-25 0.632780744539069 1102 26-27 0.523999390178183 1102 28-29 0.5216822598950372 1102 30-31 0.4227025658452206 1102 32-33 0.7199767077920214 1102 34-35 0.5001263620730327 1102 36-37 0.5905359370443293 1102 38-39 0.7659457194211683 1102 40-41 0.5588563557063964 1102 42-43 0.3320862462911336 1102 44-45 0.4738562634975878 1102 46-47 0.47219053829183366 1102 48-49 0.599480704889011 1102 50-51 0.48515627718882826 1102 52-53 0.18552792805810725 1102 54-55 -0.06063324370374801 1102 56-57 0.12498784694461307 1102 58-59 0.43621790281603623 1102 60-61 0.5053703443024773 1102 62-63 0.1525998365813308 1102 64-65 0.0038450979146524844 1102 66-67 -0.12929277298196595 1102 68-69 -6.968041027069191 1102 70-71 -7.700953828779468 1102 72-73 -1.7272085311942007 1102 74-75 -0.0934862668904124 1102 76-77 0.3609133822824191 1102 78-79 0.17815042999428954 1102 80-81 0.04155705231396567 1102 82-83 -0.09231899439556557 1102 84-85 -0.18817654735235934 1102 86-87 -0.3376881602479962 1102 88-89 -0.37289504203296886 1102 90-91 -0.5721569920687948 1102 92-93 -0.5476131246585787 1102 94-95 -0.3142654801882614 1102 96-97 -0.5691167507291333 1102 98-99 -0.8040033005570031 1102 100-101 -0.5780266609808393 1103 1 0.30335683340984687 1103 2 0.1928562658366566 1103 3 0.270993597007525 1103 4 0.28981408535280195 1103 5 0.37222016453232953 1103 6 0.23193377708693674 1103 7 0.24853705621352162 1103 8 0.41474821234737647 1103 9 0.3968641851498802 1103 10-11 0.44979825329472334 1103 12-13 0.3614809634278586 1103 14-15 0.5331508952449724 1103 16-17 0.42812224939373067 1103 18-19 0.5174599296620173 1103 20-21 0.425553168819242 1103 22-23 0.3992527758817559 1103 24-25 0.44912389108283435 1103 26-27 0.6030181050670365 1103 28-29 0.5952000060986933 1103 30-31 0.4897270408197869 1103 32-33 0.5237041901933637 1103 34-35 0.46361073633966043 1103 36-37 0.5717479878910012 1103 38-39 0.7126609592075326 1103 40-41 0.5168749440123008 1103 42-43 0.449660398935805 1103 44-45 0.6014399761119193 1103 46-47 0.5459416133299229 1103 48-49 0.5345531577215112 1103 50-51 0.3641055473681263 1103 52-53 0.21123957538886629 1103 54-55 0.137549284121107 1103 56-57 0.2075447087773412 1103 58-59 0.5753425596254012 1103 60-61 0.4100716470888237 1103 62-63 0.017663495978425203 1103 64-65 0.018019805892425467 1103 66-67 0.005097786250434666 1103 68-69 -7.224007362142423 1103 70-71 -8.03978266624718 1103 72-73 -1.4789018120947937 1103 74-75 0.1653592876114054 1103 76-77 0.42037963490081864 1103 78-79 0.18132641944665906 1103 80-81 -0.022430180399322097 1103 82-83 -0.2975686835711677 1103 84-85 -0.37579112045603225 1103 86-87 -0.2359337699492663 1103 88-89 -0.29111148899849226 1103 90-91 -0.38642312273039536 1103 92-93 -0.43395690523994546 1103 94-95 -0.1884919561765379 1103 96-97 -0.38013817334034883 1103 98-99 -0.2933508324402254 1103 100-101 -0.419236882325837 1104 1 0.2076135125298748 1104 2 0.26755595217037964 1104 3 0.26384642696284644 1104 4 0.2816887446392258 1104 5 0.3354631280197822 1104 6 0.2261075418979317 1104 7 0.3486337241812407 1104 8 0.4359459924994553 1104 9 0.3803360567519576 1104 10-11 0.47256236145428687 1104 12-13 0.31740756713750073 1104 14-15 0.5858173987697981 1104 16-17 0.5885383457632329 1104 18-19 0.5205803764983088 1104 20-21 0.49709116926421615 1104 22-23 0.5084808133269689 1104 24-25 0.5047184807308227 1104 26-27 0.6799651118640355 1104 28-29 0.5683419675489816 1104 30-31 0.38394376833200994 1104 32-33 0.5499419419868801 1104 34-35 0.3655826736747514 1104 36-37 0.7140009087007542 1104 38-39 0.6349996965577063 1104 40-41 0.6140374779664839 1104 42-43 0.6257548951060841 1104 44-45 0.8306369909034714 1104 46-47 0.8743473145854566 1104 48-49 0.8048359487594112 1104 50-51 0.6891902764222593 1104 52-53 0.47920670480231564 1104 54-55 0.5512673207370042 1104 56-57 0.469815254651607 1104 58-59 0.5382381096103437 1104 60-61 0.6597157098408282 1104 62-63 0.33522226805407485 1104 64-65 0.2819108839916282 1104 66-67 0.05020643017506998 1104 68-69 -6.6855810700881335 1104 70-71 -4.67864177557588 1104 72-73 0.015222473413103899 1104 74-75 0.688614711264993 1104 76-77 0.9931987675229657 1104 78-79 0.5943972939935307 1104 80-81 0.20435845104851325 1104 82-83 0.09330771849046826 1104 84-85 0.11600174111312356 1104 86-87 -0.030685304847143158 1104 88-89 -0.08079228361266289 1104 90-91 0.03874360815730782 1104 92-93 0.20563687157158128 1104 94-95 0.24872742643515622 1104 96-97 0.052040065851144846 1104 98-99 0.15056280882598472 1104 100-101 0.3925754489143962 1105 1 0.14112576136004407 1105 2 0.1406945428667754 1105 3 0.07725774910249328 1105 4 0.06917888134901773 1105 5 0.25617603746101736 1105 6 0.21860616562488389 1105 7 0.14713771537309128 1105 8 0.3180285230068378 1105 9 0.36742271607850086 1105 10-11 0.2989920797770722 1105 12-13 0.25992316489875833 1105 14-15 0.18653053758987426 1105 16-17 0.2570791522592373 1105 18-19 0.39149812124552597 1105 20-21 0.3354420500789672 1105 22-23 0.26301471551983013 1105 24-25 0.3590364227438485 1105 26-27 0.48931975449180243 1105 28-29 0.3300234291509696 1105 30-31 0.276611214886735 1105 32-33 0.29070737918295464 1105 34-35 0.3614380273769271 1105 36-37 0.4643347696054221 1105 38-39 0.4979429711700618 1105 40-41 0.47752254540386474 1105 42-43 0.4115806589376376 1105 44-45 0.38915118945379845 1105 46-47 0.3673136417714389 1105 48-49 0.46918623318917696 1105 50-51 0.3131974101042445 1105 52-53 0.2071947082888741 1105 54-55 0.18340146148386083 1105 56-57 0.3718497302915722 1105 58-59 0.49415092271246763 1105 60-61 0.5793505387242135 1105 62-63 0.14128612712371336 1105 64-65 0.2623728529796061 1105 66-67 0.35957930842078056 1105 68-69 -3.4507361301853905 1105 70-71 -3.7391396423526473 1105 72-73 0.41621258718501153 1105 74-75 0.9536566875930994 1105 76-77 0.9740299217572961 1105 78-79 0.5595432936265219 1105 80-81 0.4213492300190609 1105 82-83 0.43874078574434705 1105 84-85 0.440581175006141 1105 86-87 0.4542181266603329 1105 88-89 0.34559603182912113 1105 90-91 0.1961241017805726 1105 92-93 0.1967692002255852 1105 94-95 0.16467258195401158 1105 96-97 0.26219673160029444 1105 98-99 0.43353349420996423 1105 100-101 0.5534326376473082 1106 1 0.10092855668839817 1106 2 0.17573427099237193 1106 3 0.17429840262218121 1106 4 0.25055580393501486 1106 5 0.3065398130689019 1106 6 0.23360281506007397 1106 7 0.35546339027257545 1106 8 0.384443578211382 1106 9 0.3029015277448508 1106 10-11 0.3970849701976462 1106 12-13 0.17972962989333752 1106 14-15 0.33348646968509144 1106 16-17 0.34542469122152397 1106 18-19 0.4520886184055328 1106 20-21 0.398941715022481 1106 22-23 0.4475277667675144 1106 24-25 0.5270082939545304 1106 26-27 0.6153747835300294 1106 28-29 0.5771799962579962 1106 30-31 0.556628605913609 1106 32-33 0.6360914177304053 1106 34-35 0.459137146657028 1106 36-37 0.5683904687566113 1106 38-39 0.7225405045161608 1106 40-41 0.616086828382997 1106 42-43 0.44833061107242145 1106 44-45 0.7298312743347708 1106 46-47 0.7754098827568257 1106 48-49 0.820669017668088 1106 50-51 0.6138441488878357 1106 52-53 0.5758794520504367 1106 54-55 0.46649036313680625 1106 56-57 0.5137550050378934 1106 58-59 0.8574442885941451 1106 60-61 1.0447695188761017 1106 62-63 0.553629607881895 1106 64-65 0.6276511094095021 1106 66-67 0.6877109696627208 1106 68-69 -3.0978466669139664 1106 70-71 -3.315111066821345 1106 72-73 0.9557410350757536 1106 74-75 1.2355210375835242 1106 76-77 1.3993160759471017 1106 78-79 1.0927512998809092 1106 80-81 0.8196064576660085 1106 82-83 0.8071513416190008 1106 84-85 0.7744519548899866 1106 86-87 0.7259114382947729 1106 88-89 0.6982896466098971 1106 90-91 0.9082633576360841 1106 92-93 0.740365552896467 1106 94-95 0.902707954345356 1106 96-97 0.9773238935123523 1106 98-99 0.9780945702199197 1106 100-101 0.9602130639667941 1107 1 0.03044937536381198 1107 2 0.07278444666524919 1107 3 0.07230320515062516 1107 4 -0.14998885973333387 1107 5 0.2693219265813127 1107 6 0.19241133273784072 1107 7 0.09792468353732886 1107 8 0.1131724120610258 1107 9 0.2615966557128715 1107 10-11 0.08013748954267896 1107 12-13 0.15256912013695256 1107 14-15 0.16549521410810542 1107 16-17 -0.0015984486531834818 1107 18-19 0.15009319876875082 1107 20-21 0.23124029960787595 1107 22-23 0.21043727494328124 1107 24-25 0.09450623166315353 1107 26-27 0.3367868195187427 1107 28-29 0.23291678926204185 1107 30-31 0.28298142547220806 1107 32-33 0.23592118790390515 1107 34-35 0.08775286423103523 1107 36-37 0.2848304471484866 1107 38-39 0.49917751611369 1107 40-41 0.40000556137133003 1107 42-43 0.20205422831182318 1107 44-45 0.3232627716548677 1107 46-47 0.542349510725785 1107 48-49 0.39316913345034266 1107 50-51 0.39910963034293445 1107 52-53 0.07195374991007952 1107 54-55 0.1514787532951658 1107 56-57 0.42132372116730465 1107 58-59 0.6683159777556078 1107 60-61 0.6364896177020256 1107 62-63 0.24116413961999328 1107 64-65 0.10536584448346176 1107 66-67 0.34853414990314135 1107 68-69 -2.6170168336797204 1107 70-71 -3.0200355810613644 1107 72-73 1.0445654666190443 1107 74-75 1.2236881762373493 1107 76-77 0.8579924907678453 1107 78-79 0.6264400918677886 1107 80-81 0.6276169578778337 1107 82-83 0.5691045994241364 1107 84-85 0.3699456581295486 1107 86-87 0.4325781550910257 1107 88-89 0.4604917721841737 1107 90-91 0.33124294868460424 1107 92-93 0.31851161319791643 1107 94-95 0.6926834666113564 1107 96-97 0.7136208282646486 1107 98-99 0.7992820313516873 1107 100-101 0.9281290738931318 1108 1 -0.06291601883977549 1108 2 -0.05003736751772436 1108 3 0.0034188148003444496 1108 4 0.03513861492455561 1108 5 -0.05852272124319313 1108 6 -0.2661872040390634 1108 7 0.05833511167948302 1108 8 0.24641256781459475 1108 9 0.2780410933113231 1108 10-11 0.19810576430628402 1108 12-13 0.01406561115973659 1108 14-15 0.12855823967888114 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2311 4 -0.02141586984389221 2311 5 0.22861980024219974 2311 6 0.25469001020565685 2311 7 -3.0863132048723045 2311 8 -0.8957427789699821 2311 9 -3.2855663773093653 2311 10-11 -1.5275229168146112 2311 12-13 -1.0098075781141915 2311 14-15 -2.122330232689606 2311 16-17 -0.06846021379392653 2311 18-19 0.23343915737368803 2311 20-21 -1.0022087679169474 2311 22-23 -0.13836224426203358 2311 24-25 0.26952381197357766 2311 26-27 0.6841902384727092 2311 28-29 0.5143076827297008 2311 30-31 0.5440848004354777 2311 32-33 0.8705633778196287 2311 34-35 0.4359344486448151 2311 36-37 0.6275820429523904 2311 38-39 0.6964560931877841 2311 40-41 0.5434376783282033 2311 42-43 0.6254942581196374 2311 44-45 0.6818394989289231 2311 46-47 0.7643390337278149 2311 48-49 0.8130861991060172 2311 50-51 0.4667897754757533 2311 52-53 0.9882666237626196 2311 54-55 1.1109900777074486 2311 56-57 0.9747152722138637 2311 58-59 1.1531616589087825 2311 60-61 1.3134677848046437 2311 62-63 1.2606572927678812 2311 64-65 1.471428749366794 2311 66-67 1.6188695265530235 2311 68-69 3.9226619611574876 2311 70-71 5.121795940469436 2311 72-73 4.108885559236818 2311 74-75 3.039114254055267 2311 76-77 2.6117437936046066 2311 78-79 1.9496182765849426 2311 80-81 1.5533252013892032 2311 82-83 1.600593205648913 2311 84-85 1.5230547605548814 2311 86-87 1.541995052359038 2311 88-89 1.5795812056532519 2311 90-91 1.7251109797628494 2311 92-93 1.7529655192439897 2311 94-95 1.930076467434585 2311 96-97 1.8421566838838679 2311 98-99 2.048032471715075 2311 100-101 2.1440572116077696 2312 1 -0.11411864508145442 2312 2 0.009012556262813831 2312 3 0.05288218499627462 2312 4 0.15117522356553792 2312 5 -1.131674895986329 2312 6 -0.029238711103062087 2312 7 0.10049598813923666 2312 8 0.2708308839398157 2312 9 -2.3688899294015258 2312 10-11 0.03306088885977232 2312 12-13 -0.8761462617318045 2312 14-15 -3.8631560568711976 2312 16-17 -0.7730446542107572 2312 18-19 0.19024913069876703 2312 20-21 0.3614561943001391 2312 22-23 0.5462038751797067 2312 24-25 0.6591836247770075 2312 26-27 0.6474877156337868 2312 28-29 0.542935107931072 2312 30-31 0.3923329145684633 2312 32-33 0.9885353921039552 2312 34-35 0.7481869941718529 2312 36-37 0.8794077471361774 2312 38-39 0.9692522320127779 2312 40-41 0.6286168520876316 2312 42-43 0.2778561327868516 2312 44-45 1.0269918502300115 2312 46-47 1.2103538773897 2312 48-49 1.2493067144014773 2312 50-51 1.499939904522563 2312 52-53 1.3580440147351212 2312 54-55 1.4639168764285202 2312 56-57 1.289803093552223 2312 58-59 1.312879546055779 2312 60-61 1.27293279134879 2312 62-63 1.4911407658582192 2312 64-65 1.5781205609269726 2312 66-67 1.943927082873298 2312 68-69 4.513152758703498 2312 70-71 5.373287142598187 2312 72-73 3.319617591697863 2312 74-75 2.7504391444835044 2312 76-77 2.824696932825656 2312 78-79 2.1726050840402777 2312 80-81 1.966233439795321 2312 82-83 2.146812848829235 2312 84-85 1.9009763462569147 2312 86-87 1.792032838550508 2312 88-89 1.8455791086425712 2312 90-91 2.1310103304632975 2312 92-93 2.4079486572585047 2312 94-95 2.2818082880962365 2312 96-97 2.54658334031247 2312 98-99 2.902310393169632 2312 100-101 2.967524961052373 2313 1 -0.29984811037392234 2313 2 -0.1957959559455773 2313 3 -0.08414895451323545 2313 4 0.028032140784226556 2313 5 0.01789416889410944 2313 6 0.021082412655758276 2313 7 -2.8636421737797164 2313 8 -3.190217056301684 2313 9 -0.8619471704350872 2313 10-11 0.021984294081633493 2313 12-13 0.08969289305922956 2313 14-15 -0.5704363402143002 2313 16-17 -0.18038818740821227 2313 18-19 0.22642620253346735 2313 20-21 -2.560750546912324 2313 22-23 -0.5182112016087004 2313 24-25 -0.0014013576731457533 2313 26-27 -1.5226474532147165 2313 28-29 -1.5327645755996215 2313 30-31 -0.8905852749522651 2313 32-33 -0.8100124677423608 2313 34-35 -1.3373881800656733 2313 36-37 -1.9980303003338733 2313 38-39 -1.9158947828547497 2313 40-41 -0.8325160531709628 2313 42-43 0.06502088063201228 2313 44-45 0.13853526176839637 2313 46-47 0.09521282361361472 2313 48-49 -2.1628845638548952 2313 50-51 -0.3133442235721873 2313 52-53 -0.2015223014762384 2313 54-55 -0.2878785596298883 2313 56-57 -0.06926566955007019 2313 58-59 -0.05594309945697162 2313 60-61 0.3559802563278005 2313 62-63 0.5835030709083533 2313 64-65 0.6565124676536662 2313 66-67 1.2778545789407048 2313 68-69 1.8795423073970845 2313 70-71 4.382634902382112 2313 72-73 3.685695630204279 2313 74-75 2.263535943333398 2313 76-77 2.3041325213878103 2313 78-79 1.6157550249540513 2313 80-81 1.537855564144163 2313 82-83 1.500663456633763 2313 84-85 1.2786311222217641 2313 86-87 1.453667189737665 2313 88-89 0.9005198243865244 2313 90-91 1.5083595200967501 2313 92-93 1.5724649642794262 2313 94-95 1.7693509271638312 2313 96-97 1.79624991185894 2313 98-99 1.9251348402115447 2313 100-101 1.9848769501308148 2314 1 -0.20617518330932327 2314 2 -0.12011854755157714 2314 3 -0.06214396029921687 2314 4 0.0534186474308882 2314 5 0.011574148122285521 2314 6 -2.518988301311694 2314 7 -0.6311416368536271 2314 8 -0.053292889498784746 2314 9 -0.28616590290485533 2314 10-11 -0.1780333363272959 2314 12-13 -2.164578634471461 2314 14-15 -0.657658621204547 2314 16-17 -1.6550986480106644 2314 18-19 -0.18488626921780593 2314 20-21 -0.7866412832543617 2314 22-23 -0.3140005791270468 2314 24-25 -0.18375091418821654 2314 26-27 -1.9514725307083793 2314 28-29 -0.7727211727383647 2314 30-31 -0.07680871313845472 2314 32-33 0.18110661445061282 2314 34-35 -0.038727289762078954 2314 36-37 -0.44166331856371954 2314 38-39 -0.011725832248743018 2314 40-41 -0.1453198972412082 2314 42-43 -0.21910085253335865 2314 44-45 -3.3635754187333333 2314 46-47 -3.771611234521856 2314 48-49 -3.681101103742897 2314 50-51 -4.0280031072146905 2314 52-53 -0.8035233528897194 2314 54-55 -1.0852715029785784 2314 56-57 -0.6788604586422977 2314 58-59 -1.3397306562349023 2314 60-61 -0.5797812651444332 2314 62-63 -0.6219527392730484 2314 64-65 -0.8158480197149487 2314 66-67 -0.583104002420427 2314 68-69 -1.0263209255070294 2314 70-71 1.985602260481361 2314 72-73 1.7001863838561775 2314 74-75 1.3090446767968764 2314 76-77 1.1973930571599105 2314 78-79 -1.1967415691714969 2314 80-81 -0.7316433475787782 2314 82-83 0.3587925176381823 2314 84-85 0.557348493944513 2314 86-87 0.05763130704719899 2314 88-89 0.20066519512196734 2314 90-91 0.5944012047945186 2314 92-93 0.23658195326202147 2314 94-95 0.7831425549634581 2314 96-97 1.0402185489642193 2314 98-99 0.7995894996080715 2314 100-101 1.0673991243769514 2315 1 -0.383066881156104 2315 2 -0.324599616077343 2315 3 -0.42674768008406616 2315 4 -0.14452841061017097 2315 5 -0.21929433602957715 2315 6 -0.12601823763262843 2315 7 -1.0977106418299414 2315 8 -2.997603555997184 2315 9 -0.7167182087553456 2315 10-11 -1.379106534844297 2315 12-13 -0.5752464971811762 2315 14-15 -0.5105519766851501 2315 16-17 -0.2719853661344658 2315 18-19 -0.017188811264070125 2315 20-21 -0.639042294206341 2315 22-23 -0.18070942563901582 2315 24-25 -5.49948564502737 2315 26-27 -1.060212797225887 2315 28-29 -0.7134218811551207 2315 30-31 -1.1107661603823829 2315 32-33 -0.0825952979676643 2315 34-35 -2.1521880608005546 2315 36-37 -3.4280277456056574 2315 38-39 -0.9370676765880575 2315 40-41 0.020993129509449204 2315 42-43 -1.5102488833257794 2315 44-45 -4.951641307972018 2315 46-47 -3.368414150047336 2315 48-49 -2.5462882355929963 2315 50-51 -1.137417000641797 2315 52-53 -0.04451841068520679 2315 54-55 -1.220039519513989 2315 56-57 -1.149647391303482 2315 58-59 -0.6813282264029503 2315 60-61 -1.614761602060213 2315 62-63 -0.4446616403573316 2315 64-65 0.14457494976889862 2315 66-67 0.4963353694523249 2315 68-69 3.1578452608096512 2315 70-71 3.5012868530138235 2315 72-73 -2.419428267443834 2315 74-75 -1.9975529445327105 2315 76-77 -0.5028107362975547 2315 78-79 -0.36673174615631154 2315 80-81 -0.46711398256167413 2315 82-83 -0.9130447613866117 2315 84-85 -0.42252387507342704 2315 86-87 0.02759522110946122 2315 88-89 0.06409680408410523 2315 90-91 -0.0603048039575107 2315 92-93 -1.2894913305242035 2315 94-95 -0.10847786735797982 2315 96-97 -0.05557051542769287 2315 98-99 -2.8812102575451597 2315 100-101 -4.2561107958451565 2316 1 -0.5225084652502048 2316 2 -0.41590512803120205 2316 3 -0.9583213137403206 2316 4 -0.2821367431612458 2316 5 -0.2229259841656912 2316 6 -0.5404218080469576 2316 7 -0.6021970283974767 2316 8 -0.9436518562440597 2316 9 -4.956373319025715 2316 10-11 -1.3198987680524255 2316 12-13 -0.6316804238804252 2316 14-15 -0.3127685534616873 2316 16-17 -0.293692474445038 2316 18-19 -0.3968214995035666 2316 20-21 -3.8986770143310068 2316 22-23 -1.1581786710966853 2316 24-25 -0.34616476045626854 2316 26-27 -0.5224877600043598 2316 28-29 0.058650015892858676 2316 30-31 0.10983210502934782 2316 32-33 -1.2010176639137384 2316 34-35 -0.29378411648767155 2316 36-37 -0.06027128743892263 2316 38-39 -5.22463675771256 2316 40-41 -5.066275644535018 2316 42-43 -3.633409938147466 2316 44-45 -2.038454789232926 2316 46-47 -2.4367076715023437 2316 48-49 -3.021559143710995 2316 50-51 -1.4067753606213458 2316 52-53 -0.9778638640352213 2316 54-55 -0.26025470526116123 2316 56-57 -3.9899785489749995 2316 58-59 -0.8098776386415523 2316 60-61 -0.6383112697697584 2316 62-63 -0.21328368951944654 2316 64-65 0.13111678026373497 2316 66-67 0.2130121559368412 2316 68-69 2.760684493541298 2316 70-71 3.9226427977037694 2316 72-73 2.577894337694339 2316 74-75 1.319993076873775 2316 76-77 -1.4162100484668052 2316 78-79 0.22765585435368152 2316 80-81 0.25963202268478724 2316 82-83 0.5828208672362756 2316 84-85 0.7693527233527959 2316 86-87 0.7018223423005203 2316 88-89 0.6331224775272872 2316 90-91 0.7140664305691651 2316 92-93 0.75482455988708 2316 94-95 0.8503466534161639 2316 96-97 0.8539217746969889 2316 98-99 0.9444091406603654 2316 100-101 0.9124331861506754 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 38.0 4 108.0 5 213.0 6 590.0 7 1567.0 8 3320.0 9 5637.0 10 8455.0 11 12406.0 12 16123.0 13 20662.0 14 25282.0 15 31365.0 16 37508.0 17 41767.0 18 40527.0 19 37512.0 20 37692.0 21 38429.0 22 39941.0 23 44289.0 24 52642.0 25 64849.0 26 80770.0 27 100363.0 28 121662.0 29 144073.0 30 168799.0 31 194423.0 32 223711.0 33 256937.0 34 289708.0 35 318000.0 36 311445.0 37 229139.0 38 78816.0 39 3679.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.81689094715036 19.38599790186259 15.528747824715644 23.2683633262714 2 16.691358889946663 20.494707557767146 37.89289350675645 24.921040045529736 3 19.806413478127435 23.67334253381892 29.782160011886667 26.738083976166976 4 14.135281806251019 16.89947861638855 34.76204607838872 34.203193498971714 5 14.633298901914577 36.92461465792212 32.93127298491852 15.51081345524478 6 33.57793266148811 35.129293005317834 16.19526766967639 15.097506663517663 7 29.556172091345346 32.47942549707117 19.232164222295467 18.732238189288022 8 27.45901320245934 34.62446828113686 18.333887004388707 19.582631512015094 9 26.6247109447791 16.42709579633902 19.13707770686751 37.81111555201437 10-11 26.07872681165627 26.044237922075226 25.757603778139433 22.119431488129074 12-13 26.675563252159968 23.23134539471123 26.81769017951919 23.275401173609612 14-15 23.592518433353142 24.61113800164544 25.01967586885263 26.776667696148788 16-17 23.032547613285328 27.706023608585152 25.685090827455408 23.57633795067411 18-19 22.99398839268784 26.063831944261285 28.232510239002796 22.709669424048084 20-21 23.915021009221217 25.00081104158502 28.43596145411321 22.648206495080547 22-23 23.7434260875038 24.928101000211196 28.348757757224856 22.97971515506015 24-25 23.403848403008944 25.118031134427426 28.368674678860415 23.10944578370321 26-27 23.16821857153378 25.432925818395823 28.291775634168964 23.107079975901435 28-29 23.22574355587075 25.53093058761012 28.32299188494228 22.92033397157686 30-31 23.311505458320493 25.388311245924513 28.129118071663772 23.171065224091226 32-33 23.229900411444532 25.27122653942949 28.10577360574806 23.393099443377917 34-35 23.493753495693564 25.444496496844632 28.126009776911488 22.935740230550316 36-37 23.589128650322365 25.274407350259505 28.111200080007066 23.025263919411067 38-39 23.394247243319214 25.43975699383132 28.00287910611971 23.163116656729756 40-41 23.541534798631726 25.355043269490668 27.964362310128294 23.139059621749308 42-43 23.334022765549644 25.434106893597015 27.795147068230087 23.436723272623254 44-45 23.31031829265521 25.496936559010607 27.906015513641808 23.286729634692374 46-47 23.657351842739637 25.7658702206216 27.370835168478052 23.20594276816071 48-49 23.62149211456786 25.877587210845437 27.13133772402229 23.36958295056441 50-51 23.608731577212964 26.080108178832727 27.06615239514494 23.24500784880937 52-53 23.789780358454355 25.94333061469606 26.796820411690952 23.470068615158635 54-55 23.58052791863344 26.03228468558825 26.871916965080512 23.515270430697797 56-57 23.482458907230225 26.122384607296816 26.89720219992186 23.497954285551103 58-59 23.60906637471238 25.92232168555576 26.880442707919272 23.58816923181259 60-61 23.560405459906146 25.907559008978172 27.14982425549905 23.38221127561663 62-63 23.527652239023624 25.920298963642235 27.100392991587835 23.451655805746306 64-65 23.389752588327074 26.019080532526306 26.993790091431542 23.597376787715085 66-67 23.192425132854424 26.067039510979388 27.176392426627654 23.564142929538534 68-69 23.09849706575897 25.472566292989956 28.111536139172372 23.3174005020787 70-71 22.638018799017004 25.488293226285876 28.356774933945395 23.516913040751724 72-73 23.244401574954434 26.06539167564093 27.041987821489965 23.648218927914673 74-75 23.286988233976615 26.370742941724952 26.565926329875506 23.776342494422924 76-77 22.744598341372242 26.17845097321127 27.185594558345944 23.891356127070544 78-79 23.350859745977875 26.172130894885328 26.61919231411917 23.857817045017626 80-81 23.305295829380057 26.191445222401867 26.560107605877896 23.943151342340176 82-83 23.41875700144141 26.304835094460454 26.457394738711805 23.819013165386334 84-85 23.265743754364422 26.36426676485688 26.34718620968206 24.022803271096635 86-87 23.361571633768843 26.26603155498277 26.41583377873879 23.9565630325096 88-89 23.330702843605355 26.224706543593268 26.235185246764537 24.209405366036833 90-91 23.15757901466215 26.310152065479176 26.35607521403902 24.176193705819653 92-93 23.109690607998296 26.200869183094095 26.357309811653206 24.332130397254407 94-95 23.044618323250287 26.10073368717335 26.54473829378202 24.309909695794342 96-97 23.047339380939817 25.844900995352816 26.625095457349836 24.482664166357534 98-99 22.981497554367664 26.025778506014714 26.333190029282694 24.659533910334925 100-101 22.809053109390387 25.94277806708879 26.554419401466888 24.693749422053934 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1738.0 1 1257.0 2 779.0 3 799.5 4 785.0 5 865.0 6 1320.5 7 2447.0 8 3730.0 9 4556.5 10 4485.0 11 3845.0 12 3630.0 13 3791.0 14 4018.0 15 4236.0 16 4493.5 17 4789.0 18 5080.0 19 5429.5 20 5960.0 21 6579.0 22 7453.0 23 8786.0 24 10288.0 25 11975.5 26 14253.0 27 17076.5 28 19873.5 29 22614.5 30 26139.5 31 30394.0 32 34841.0 33 38167.5 34 40515.0 35 43342.0 36 47991.5 37 53377.0 38 58632.0 39 63181.5 40 67627.5 41 73177.5 42 78245.5 43 84490.5 44 91912.5 45 99124.0 46 105749.5 47 111618.5 48 116648.0 49 119805.5 50 120774.5 51 121680.0 52 125144.0 53 129159.0 54 130332.5 55 126810.0 56 119871.0 57 111151.0 58 102213.0 59 91788.5 60 77911.0 61 63368.0 62 49614.0 63 37602.0 64 28146.0 65 20669.0 66 14878.0 67 10995.0 68 8284.5 69 6174.5 70 4485.5 71 3136.5 72 2214.5 73 1670.0 74 1225.5 75 798.5 76 513.5 77 301.5 78 186.0 79 125.0 80 71.5 81 46.5 82 27.0 83 19.0 84 9.5 85 4.0 86 3.0 87 1.5 88 3.0 89 3.0 90 0.5 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.021898122795588972 2 0.016675014988048492 3 3.244166340087255E-4 4 0.08350484159384594 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.009862265673865256 12-13 0.0 14-15 0.0 16-17 5.839499412157059E-4 18-19 8.110415850218138E-5 20-21 0.0 22-23 2.2709164380610786E-4 24-25 0.003649687132598162 26-27 0.0037307912911003433 28-29 0.001232783209233157 30-31 1.946499804052353E-4 32-33 7.62379089920505E-4 34-35 0.002984633032880275 36-37 0.08850085775758032 38-39 0.09823335677784208 40-41 0.08157456262149403 42-43 0.0852242497540922 44-45 0.08389414155465641 46-47 0.0825478125235202 48-49 0.0771300547355745 50-51 0.07691918392346882 52-53 0.06061724806453036 54-55 0.05137137399528169 56-57 0.028937963753578315 58-59 0.022546956063606422 60-61 0.013787706945370834 62-63 0.002416903923365005 64-65 0.0066343201654784365 66-67 0.16614997910756876 68-69 0.7647473313487686 70-71 0.9321300936655705 72-73 0.37752363699595387 74-75 0.057210873407438745 76-77 0.2102057580059537 78-79 0.014306773559784793 80-81 0.003130620518184201 82-83 0.0017842914870479904 84-85 1.1354582190305393E-4 86-87 0.001897837308951044 88-89 4.3796245591177943E-4 90-91 0.004298520400615613 92-93 0.0044282870542191035 94-95 0.08217473339441017 96-97 0.04013033762687934 98-99 0.013982356925776068 100-101 0.013657940291767343 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3082456.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.28274747868497 #Duplication Level Percentage of deduplicated Percentage of total 1 67.41259173035426 28.50389593018256 2 14.618409595972349 12.362130429729676 3 6.489763853324554 8.232151386192589 4 3.335127711316408 5.640734513070249 5 2.013069672545761 4.255905831062573 6 1.3450682606439965 3.412390896384247 7 0.9435635504515951 2.792752153368535 8 0.6271924705209019 2.121553668125429 9 0.48839843880511097 1.8585745071282431 >10 2.493599184627383 18.690298447686637 >50 0.15512976598582406 4.497767720752862 >100 0.07435122624856685 5.368352411294803 >500 0.0029565102026617614 0.8553272979328194 >1k 5.446203004175652E-4 0.3822326105259728 >5k 7.780290005965216E-5 0.2496897765635712 >10k+ 1.5560580011930432E-4 0.776242419999213 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12429 0.40321743440944496 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11167 0.36227605519754374 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7590 0.24623222521262264 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 9.732499020261765E-5 2 0.0 0.0 0.0 0.0 1.297666536034902E-4 3 0.0 0.0 0.0 0.0 1.297666536034902E-4 4 0.0 0.0 0.0 0.0 1.297666536034902E-4 5 0.0 0.0 0.0 0.0 1.297666536034902E-4 6 0.0 0.0 0.0 0.0 1.6220831700436276E-4 7 0.0 0.0 0.0 0.0 1.6220831700436276E-4 8 0.0 0.0 0.0 0.0 1.6220831700436276E-4 9 0.0 0.0 0.0 3.244166340087255E-5 1.6220831700436276E-4 10-11 0.0 0.0 0.0 8.110415850218138E-5 1.6220831700436276E-4 12-13 0.0 0.0 0.0 9.732499020261765E-5 2.7575413890741667E-4 14-15 0.0 0.0 0.0 1.4598748530392648E-4 3.4063746570916174E-4 16-17 0.0 0.0 0.0 2.1087081210567157E-4 3.892999608104706E-4 18-19 0.0 0.0 0.0 2.2709164380610786E-4 4.866249510130883E-4 20-21 0.0 0.0 0.0 2.7575413890741667E-4 6.163916046165785E-4 22-23 0.0 0.0 0.0 3.8929996081047064E-4 7.299374265196325E-4 24-25 0.0 0.0 0.0 5.677291095152696E-4 7.461582582200687E-4 26-27 0.0 0.0 0.0 0.0012003415458322845 8.434832484226863E-4 28-29 0.0 0.0 0.0 0.00235202059656326 9.083665752244314E-4 30-31 0.0 0.0 0.0 0.005758395253654878 9.40808238625304E-4 32-33 0.0 0.0 0.0 0.013479511143062544 0.0010056915654270491 34-35 0.0 0.0 0.0 0.02429880588725354 0.0010705748922287942 36-37 0.0 0.0 0.0 0.044007116403283614 0.0010867957239292306 38-39 0.0 0.0 0.0 0.07187450526463314 0.0011678998824314118 40-41 0.0 0.0 0.0 0.10235344802975291 0.0011678998824314118 42-43 0.0 0.0 0.0 0.1328972741216744 0.0011678998824314118 44-45 0.0 0.0 0.0 0.17125954109320618 0.0012165623775327206 46-47 0.0 0.0 0.0 0.2187865779754845 0.0013138873677353384 48-49 0.0 0.0 0.0 0.27171515181400807 0.0014923165164401374 50-51 0.0 0.0 0.0 0.32738504620990533 0.0015571998432418823 52-53 0.0 0.0 0.0 0.38427150298333534 0.0016545248334445001 54-55 0.0 0.0 0.0 0.44785716324904556 0.001719408160246245 56-57 0.0 0.0 0.0 0.5223432224174489 0.001768070655347554 58-59 0.0 0.0 0.0 0.598305377270592 0.0017842914870479904 60-61 0.0 0.0 3.244166340087255E-5 0.6758896152937788 0.0018167331504488628 62-63 0.0 0.0 3.244166340087255E-5 0.7554041322893174 0.0018491748138497353 64-65 0.0 3.244166340087255E-5 3.244166340087255E-5 0.839979548775392 0.001881616477250608 66-67 0.0 3.244166340087255E-5 3.244166340087255E-5 0.9312866104171479 0.0019140581406514804 68-69 0.0 3.244166340087255E-5 3.244166340087255E-5 1.0357487665679574 0.0019140581406514804 70-71 0.0 3.244166340087255E-5 3.244166340087255E-5 1.166456228410073 0.0019140581406514804 72-73 0.0 3.244166340087255E-5 3.244166340087255E-5 1.3115353471387752 0.001995162299153662 74-75 0.0 3.244166340087255E-5 3.244166340087255E-5 1.4673364356214655 0.002043824794254971 76-77 0.0 3.244166340087255E-5 3.244166340087255E-5 1.6519132795407299 0.002043824794254971 78-79 0.0 3.244166340087255E-5 3.244166340087255E-5 1.8692075409997742 0.002076266457655843 80-81 0.0 3.244166340087255E-5 3.244166340087255E-5 2.1009221218405063 0.002108708121056716 82-83 0.0 3.244166340087255E-5 3.244166340087255E-5 2.34718678871653 0.002189812279558897 84-85 0.0 3.244166340087255E-5 3.244166340087255E-5 2.59705248023005 0.0022060331112593334 86-87 0.0 3.244166340087255E-5 3.244166340087255E-5 2.8612736077984566 0.0022709164380610783 88-89 0.0 3.244166340087255E-5 3.244166340087255E-5 3.1486418622033856 0.0023357997648628236 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6830 0.0 51.146984 1 GTATCAA 11945 0.0 41.76183 1 TATCAAC 15775 0.0 31.523026 2 ATCAACG 16140 0.0 30.541584 3 TCAACGC 16235 0.0 30.311806 4 CAACGCA 16535 0.0 29.677942 5 AACGCAG 16980 0.0 28.928083 6 TGGTATC 3250 0.0 27.301985 2 GTGGTAT 3440 0.0 26.629423 1 ACGCAGA 19475 0.0 25.051603 7 CGCAGAG 19765 0.0 24.61207 8 GCAGAGT 20945 0.0 22.297363 9 GTATAGG 635 0.0 21.676401 1 AGAGTAC 18695 0.0 21.06037 10-11 GTACATG 13975 0.0 21.057304 1 GAGTACT 11610 0.0 20.904352 12-13 AGTACTT 11830 0.0 20.77605 12-13 TACATGG 13840 0.0 20.742222 2 GTACTTT 11975 0.0 20.089056 14-15 CAGAGTA 19955 0.0 19.481121 10-11 >>END_MODULE