FastQCFastQC Report
Fri 27 May 2016
SRR939511_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939511_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2934391
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162390.55340273330991No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123860.42209780496191546No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86680.2953934905062073No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50510.17213111681435772No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30780.10489399674412851No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA102600.045.99471
GTATCAA177850.041.0274661
TATCAAC229700.031.5994472
ATCAACG230550.031.3365823
TCAACGC234750.030.7744264
CAACGCA235950.030.2268415
AACGCAG243450.029.185336
GTACATG169750.028.1545561
TACATGG172900.027.1770152
ACGCAGA268350.025.9842247
CGCAGAG269350.025.7962258
ACATGGG177950.024.7957383
GCAGAGT269300.024.386279
GAGTACT152600.023.08115612-13
CATGGGA116050.022.7306984
GTACTTT164500.021.56534414-15
AGTACTT156850.021.26422112-13
AGAGTAC246000.020.57720610-11
CAGAGTA266800.020.52875310-11
CAACGCG3653.6379788E-1219.857515
Produced by FastQC (version 0.11.5)