FastQCFastQC Report
Fri 27 May 2016
SRR939511_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939511_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2934391
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT221510.7548755431706272No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT198260.6756427483590292No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141000.4805085620832397No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA75640.2577706924537323No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49010.16701932360070623No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45300.1543761550522749No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA114850.047.8642351
GTATCAA191250.036.697611
ATCAACG249100.027.8830813
TATCAAC254400.027.4721362
TCAACGC253200.027.450294
CAACGCA257350.026.9523985
AACGCAG264400.026.2158166
TACCGTA1301.022936E-525.5114547
GTACATG186650.024.8818231
TACATGG189400.023.8663462
ACGCAGA301350.022.9384787
CGCAGAG302150.022.8307048
ACATGGG188950.022.7175673
CATTCCG5850.021.866969
GAGTACT178800.021.68934812-13
GCAGAGT313700.021.2802649
AGTACTT182750.021.10388212-13
GTACTTT186000.020.72239514-15
CATGGGA126400.020.2032954
GTATAGG9150.020.1988981
Produced by FastQC (version 0.11.5)