##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939511_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2934391 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.99818497262294 31.0 31.0 31.0 30.0 34.0 2 31.25466340375226 31.0 31.0 34.0 30.0 34.0 3 31.236265037617688 31.0 31.0 34.0 28.0 34.0 4 35.18546710373634 35.0 35.0 37.0 33.0 37.0 5 34.820465643467415 35.0 35.0 37.0 32.0 37.0 6 34.782469684510346 35.0 35.0 37.0 32.0 37.0 7 34.64977605233931 35.0 35.0 37.0 30.0 37.0 8 34.665684975178834 35.0 35.0 37.0 30.0 37.0 9 35.99662280861685 37.0 35.0 39.0 30.0 39.0 10-11 35.86241097386136 37.0 35.0 39.0 30.0 39.0 12-13 35.82513884482334 37.0 35.0 39.0 30.0 39.0 14-15 36.65626513303782 38.0 35.5 40.0 31.0 41.0 16-17 36.682339027075805 38.0 35.5 40.0 31.0 41.0 18-19 36.67996528070049 38.0 35.0 40.0 30.0 41.0 20-21 36.60183441811265 38.0 35.0 40.0 30.0 41.0 22-23 36.46289758249668 38.0 35.0 40.0 30.0 41.0 24-25 36.24331539321106 38.0 34.5 40.0 29.5 41.0 26-27 35.87512826341138 38.0 34.0 40.0 28.5 41.0 28-29 35.59095754451265 38.0 34.0 40.0 27.0 41.0 30-31 35.33594415331835 38.0 34.0 40.0 27.0 41.0 32-33 35.081025330298516 37.0 33.0 40.0 27.0 41.0 34-35 34.990282651493956 37.5 33.0 40.0 26.5 41.0 36-37 34.939739114521544 38.0 33.0 40.0 25.5 41.0 38-39 34.8965923423293 38.0 33.0 40.0 26.0 41.0 40-41 34.64994167443943 38.0 33.0 40.0 25.0 41.0 42-43 34.31709577898787 37.0 33.0 40.0 24.0 41.0 44-45 34.082860293669114 37.0 33.0 40.0 24.0 41.0 46-47 33.68985114798948 37.0 32.5 40.0 23.0 41.0 48-49 33.335352889236646 37.0 32.0 40.0 21.0 41.0 50-51 33.06177772491805 37.0 31.5 40.0 19.5 41.0 52-53 32.70257729798108 36.5 31.0 39.5 18.0 41.0 54-55 32.4826800518404 36.0 31.0 39.0 17.5 41.0 56-57 32.10241392506997 36.0 30.5 39.0 14.5 40.0 58-59 31.688986402970837 35.0 30.0 39.0 12.5 40.0 60-61 31.41420025483993 35.0 29.5 39.0 10.5 40.0 62-63 31.039016613668732 35.0 29.0 38.0 8.0 40.0 64-65 30.620475594424875 34.5 28.5 38.0 7.5 40.0 66-67 28.984983766648682 33.5 25.0 37.5 4.5 39.5 68-69 25.386285604065716 30.0 15.5 35.0 2.0 39.0 70-71 24.80427983182882 29.5 14.0 35.0 2.0 38.0 72-73 26.599824290627936 31.0 21.5 35.0 2.0 38.0 74-75 27.193389360858866 32.0 23.5 35.0 2.0 38.0 76-77 27.21332791028871 31.5 25.0 34.5 2.0 36.5 78-79 28.035447730040065 33.0 26.0 35.0 2.0 37.0 80-81 27.888759371194908 33.0 26.0 35.0 2.0 37.0 82-83 27.4871373651296 33.0 26.0 35.0 2.0 36.0 84-85 27.145966403250284 32.5 25.5 35.0 2.0 36.0 86-87 26.69731862590909 32.0 25.0 35.0 2.0 35.5 88-89 26.206401260091106 32.0 24.0 34.0 2.0 35.0 90-91 25.76075274222147 32.0 23.0 34.0 2.0 35.0 92-93 25.21473518696043 31.5 20.5 34.0 2.0 35.0 94-95 23.87255055648685 31.0 10.0 34.0 2.0 35.0 96-97 23.08971026696851 30.0 4.0 34.0 2.0 35.0 98-99 22.769656293247902 30.0 2.0 34.0 2.0 35.0 100-101 21.539142534174893 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.36870536125886133 1101 2 0.27450080594685744 1101 3 0.3679886193506867 1101 4 0.318043388913523 1101 5 0.3918704985267567 1101 6 0.5071734069381222 1101 7 0.5308910031613081 1101 8 0.5736425416762003 1101 9 0.6108382977019318 1101 10-11 0.7314735646729318 1101 12-13 0.6069270986806643 1101 14-15 0.7850983893380814 1101 16-17 0.7716584302837575 1101 18-19 0.8433508494295978 1101 20-21 0.6847622401699383 1101 22-23 0.791621187363539 1101 24-25 0.7449452158658616 1101 26-27 0.8252937688974598 1101 28-29 0.7270346649467427 1101 30-31 0.7483014671417365 1101 32-33 0.6732340919777258 1101 34-35 0.6328268427883685 1101 36-37 0.6535561755900972 1101 38-39 0.6369623339206001 1101 40-41 0.8687035651263315 1101 42-43 0.9056192987612377 1101 44-45 0.5212175199748899 1101 46-47 0.4040616317542458 1101 48-49 0.5252700438293019 1101 50-51 0.4658442963876581 1101 52-53 0.6173226799819886 1101 54-55 0.4364750181641668 1101 56-57 0.40816188781959184 1101 58-59 0.281148162032288 1101 60-61 0.07551422700103316 1101 62-63 0.10800484205001837 1101 64-65 -0.2056922833271102 1101 66-67 -3.150084062368947 1101 68-69 -8.220735787824712 1101 70-71 -11.509802844323676 1101 72-73 -11.449024731831273 1101 74-75 -9.84991427488664 1101 76-77 -4.946492970243067 1101 78-79 -2.011218370027077 1101 80-81 -1.3318303702293015 1101 82-83 -1.0580373879373077 1101 84-85 -1.0514232258143217 1101 86-87 -1.0234858076195401 1101 88-89 -1.1776190426168185 1101 90-91 -1.3129003242858879 1101 92-93 -1.7775881663796405 1101 94-95 -5.156520791002482 1101 96-97 -2.298766054871681 1101 98-99 -1.4309080586829026 1101 100-101 -1.2767600577263565 1102 1 0.43844055510521684 1102 2 0.33757294197178567 1102 3 0.45343572859795955 1102 4 0.2407791935692103 1102 5 0.4276335045050459 1102 6 0.5171996988157943 1102 7 0.5781559590309371 1102 8 0.5699585729923271 1102 9 0.6333415846843664 1102 10-11 0.5979673279671971 1102 12-13 0.6332666087271619 1102 14-15 0.5954138201975709 1102 16-17 0.6371731665566855 1102 18-19 0.7550734213430417 1102 20-21 0.794478866845985 1102 22-23 0.7223123455997893 1102 24-25 0.6657077858361831 1102 26-27 0.810995992577908 1102 28-29 0.8041426241029441 1102 30-31 0.6058454203284143 1102 32-33 0.6194170711823332 1102 34-35 0.9355236316991906 1102 36-37 1.0567369233895505 1102 38-39 0.9737446429072989 1102 40-41 0.8236650371529421 1102 42-43 1.024325254988419 1102 44-45 0.8370988214845525 1102 46-47 0.8308492246000512 1102 48-49 0.8401451922511924 1102 50-51 0.7797713265721526 1102 52-53 0.986236486582392 1102 54-55 0.9546699889815002 1102 56-57 0.7726886877759434 1102 58-59 0.7149688719727116 1102 60-61 0.762457868889971 1102 62-63 0.7776577816993431 1102 64-65 0.6947244427348558 1102 66-67 -2.428090705617276 1102 68-69 -7.7306890135271225 1102 70-71 -10.867338385941133 1102 72-73 -8.89317056833346 1102 74-75 -7.218858741407995 1102 76-77 -3.617986898782359 1102 78-79 -0.9002096466822955 1102 80-81 -0.2665872032107792 1102 82-83 -0.20644728280961644 1102 84-85 -0.23690554419808763 1102 86-87 -0.08689485436675781 1102 88-89 -0.19778331608420174 1102 90-91 -0.36496306005868817 1102 92-93 -0.7407808317301914 1102 94-95 -4.186438353642295 1102 96-97 -1.032224330701073 1102 98-99 -0.20797982320515374 1102 100-101 -0.17441121910027135 1103 1 0.2701612213886726 1103 2 0.16952279312818774 1103 3 0.3084286875401787 1103 4 0.19284080594028552 1103 5 0.3151237880010882 1103 6 0.2736423835150461 1103 7 0.4318485810305006 1103 8 0.4348832009612096 1103 9 0.5693655133765176 1103 10-11 0.5928473729551911 1103 12-13 0.43238626231335076 1103 14-15 0.5609270463261211 1103 16-17 0.5678886517431962 1103 18-19 0.6445090501858317 1103 20-21 0.7254634544066718 1103 22-23 0.6940314774934393 1103 24-25 0.7674649931844328 1103 26-27 0.6247064664629036 1103 28-29 0.8400653570614764 1103 30-31 0.8257057597011652 1103 32-33 0.6720799384817653 1103 34-35 0.7843518680544221 1103 36-37 0.7899232824305358 1103 38-39 0.8147265589161989 1103 40-41 0.8056949569752376 1103 42-43 0.8981557695620168 1103 44-45 0.5438132567434266 1103 46-47 0.6224835678266984 1103 48-49 0.5913250951718325 1103 50-51 0.39995279737435396 1103 52-53 0.7127293621846533 1103 54-55 0.7439863910632809 1103 56-57 0.5923785112665989 1103 58-59 0.7381396184027231 1103 60-61 0.6933973788821994 1103 62-63 0.4489985930410576 1103 64-65 0.30177334771017783 1103 66-67 -2.735674329861567 1103 68-69 -7.618443141970349 1103 70-71 -11.407817555625954 1103 72-73 -9.28976992825146 1103 74-75 -7.623271903512773 1103 76-77 -3.9799760326543634 1103 78-79 -1.1510303894515808 1103 80-81 -0.545828914779765 1103 82-83 -0.3032407001994706 1103 84-85 -0.34308444236600977 1103 86-87 -0.29546350623785855 1103 88-89 -0.17127926358158874 1103 90-91 -0.09018110697519077 1103 92-93 -0.03101553105988586 1103 94-95 0.8859298368214326 1103 96-97 0.9427684257328472 1103 98-99 0.7269561101789641 1103 100-101 0.7506709330644661 1104 1 0.4093077891066699 1104 2 0.24960937321476706 1104 3 0.2963693183379519 1104 4 0.18882101620621228 1104 5 0.4694218708705975 1104 6 0.6026467125193733 1104 7 0.5286327492432434 1104 8 0.3693297068362895 1104 9 0.6590377459058985 1104 10-11 0.7097304898383072 1104 12-13 0.6591951676936816 1104 14-15 0.757742135998356 1104 16-17 0.6608076375193193 1104 18-19 0.7250594521647997 1104 20-21 0.7457170103745128 1104 22-23 0.6796529988292477 1104 24-25 0.7897284440193104 1104 26-27 0.774830152300872 1104 28-29 0.9540109352927715 1104 30-31 0.8833742816553993 1104 32-33 0.7370150034168219 1104 34-35 0.8050692459146589 1104 36-37 0.8155880938096303 1104 38-39 0.8045224772835482 1104 40-41 0.873412953264662 1104 42-43 0.9354161282509423 1104 44-45 0.8268992184008184 1104 46-47 0.6937575319578073 1104 48-49 0.7862848972744914 1104 50-51 0.7977573737503576 1104 52-53 0.9554628192038237 1104 54-55 0.8747841647182 1104 56-57 0.9439555057007389 1104 58-59 0.8788322590953648 1104 60-61 0.7249372675649433 1104 62-63 0.850668351853674 1104 64-65 0.5293552895778326 1104 66-67 -2.620491893250559 1104 68-69 -7.242747409131759 1104 70-71 -8.64312986236683 1104 72-73 -7.79116139392864 1104 74-75 -6.448874871972887 1104 76-77 -3.0971683641324113 1104 78-79 -0.35913181183871856 1104 80-81 0.32739310129939625 1104 82-83 0.6083239256508683 1104 84-85 0.45608067822768206 1104 86-87 0.49890878813443607 1104 88-89 0.40571517055570183 1104 90-91 0.3819895794812105 1104 92-93 0.4558428486556352 1104 94-95 1.4534932258133928 1104 96-97 1.7955822785467 1104 98-99 1.3524972357200902 1104 100-101 1.2590197271275443 1105 1 0.46599088475080563 1105 2 0.44332138510859664 1105 3 0.42585214911948555 1105 4 0.3155552728105988 1105 5 0.48359576816137917 1105 6 0.6264718532276348 1105 7 0.6878876357708563 1105 8 0.566724183451548 1105 9 0.9134901237348956 1105 10-11 0.9885514764515051 1105 12-13 0.8664111119226092 1105 14-15 0.9764037053092736 1105 16-17 0.9921966669603037 1105 18-19 0.976974083949564 1105 20-21 1.1389036697949422 1105 22-23 1.1626040304815817 1105 24-25 1.1305391484663758 1105 26-27 1.2101184090177028 1105 28-29 1.3075853310749608 1105 30-31 1.110859398750911 1105 32-33 1.2063277974049456 1105 34-35 1.3340573333442975 1105 36-37 1.310432955537614 1105 38-39 1.2430121982408053 1105 40-41 1.3248287813038644 1105 42-43 1.3575877517212689 1105 44-45 1.250759028624266 1105 46-47 1.246369302102046 1105 48-49 1.220203551972368 1105 50-51 1.287165993029106 1105 52-53 1.3959764902369827 1105 54-55 1.245871350740451 1105 56-57 1.3302301257415898 1105 58-59 1.4375658535432443 1105 60-61 1.3988579836934498 1105 62-63 1.530576586956709 1105 64-65 1.4365752942004342 1105 66-67 2.21382998977262 1105 68-69 -4.715805135436238 1105 70-71 -4.901833160031167 1105 72-73 -1.1416655074197664 1105 74-75 1.490109904674231 1105 76-77 1.6456962748361228 1105 78-79 1.544646228302959 1105 80-81 1.5312545356803078 1105 82-83 1.6685317553392203 1105 84-85 1.4987038799417896 1105 86-87 1.5859120926702097 1105 88-89 1.6885169046561401 1105 90-91 1.5575282063185405 1105 92-93 1.6517662134101698 1105 94-95 2.649678954466655 1105 96-97 2.7688164758068723 1105 98-99 2.449459436578394 1105 100-101 2.385461947725606 1106 1 0.136752605940611 1106 2 0.10697257285166373 1106 3 0.046084937039509555 1106 4 0.04921943841070231 1106 5 0.29466350779856754 1106 6 0.3899374177793433 1106 7 0.3208563744446167 1106 8 0.2589487668955499 1106 9 0.5677583702837694 1106 10-11 0.40087864010170904 1106 12-13 0.32500567465671537 1106 14-15 0.44120214270476055 1106 16-17 0.35318204371137796 1106 18-19 0.383194350024084 1106 20-21 0.4101339782921585 1106 22-23 0.43935438291562434 1106 24-25 0.5511568967004266 1106 26-27 0.5787847534536255 1106 28-29 0.7429795137745714 1106 30-31 0.659773866453996 1106 32-33 0.6803989514378586 1106 34-35 0.9043099784771087 1106 36-37 0.6303981911881991 1106 38-39 0.6764726233334883 1106 40-41 0.7959185546573551 1106 42-43 0.694243634759907 1106 44-45 0.6506387471845372 1106 46-47 0.360567213908773 1106 48-49 0.3130254097771825 1106 50-51 0.15872182547546743 1106 52-53 0.4103170642461649 1106 54-55 0.4926350262121133 1106 56-57 0.40807935590025934 1106 58-59 0.49616515551347007 1106 60-61 0.4944998735776771 1106 62-63 0.5887445997810126 1106 64-65 0.6724493618746727 1106 66-67 1.165099074177391 1106 68-69 -5.377504373622688 1106 70-71 -6.2297962764595525 1106 72-73 -2.3242772780590215 1106 74-75 0.3913916161579287 1106 76-77 0.5653248008208074 1106 78-79 0.49962535094161 1106 80-81 0.5625276167164905 1106 82-83 0.661858528375614 1106 84-85 0.6741567452065205 1106 86-87 0.6308601555867419 1106 88-89 0.6063369642566983 1106 90-91 0.400579445950644 1106 92-93 0.5368495330062473 1106 94-95 1.524236831781451 1106 96-97 1.4394190407598302 1106 98-99 0.9797399368514128 1106 100-101 1.0047926948167394 1107 1 -0.037962985231771995 1107 2 -0.09859067235370134 1107 3 -0.09191063479162409 1107 4 -0.07614741135619596 1107 5 0.06687329163028721 1107 6 0.07670930406317211 1107 7 0.022680368290856734 1107 8 -0.018156866740511646 1107 9 0.1525686510909381 1107 10-11 0.045649426791072756 1107 12-13 0.12907319067320344 1107 14-15 0.030080856175423776 1107 16-17 -0.1045737542718399 1107 18-19 -0.017423785149262017 1107 20-21 0.11641445203060385 1107 22-23 0.14289307955053232 1107 24-25 0.28989932576914157 1107 26-27 0.34576845585196025 1107 28-29 0.23222676708003576 1107 30-31 0.21591830404904755 1107 32-33 0.2091348322095925 1107 34-35 0.14742066016456334 1107 36-37 0.1812831756701243 1107 38-39 -0.0779861481762012 1107 40-41 -0.013929184547400553 1107 42-43 -0.13191941840791088 1107 44-45 -0.055249334800372196 1107 46-47 -0.2673200809055274 1107 48-49 -0.11812303814848235 1107 50-51 -0.17225491346117394 1107 52-53 0.0564478304331999 1107 54-55 -0.07273479501881042 1107 56-57 0.09903272797720675 1107 58-59 0.020668180066625297 1107 60-61 0.07731577877804341 1107 62-63 0.22234632926473452 1107 64-65 0.12762857018193685 1107 66-67 0.8256600124878091 1107 68-69 -5.778313190468218 1107 70-71 -6.149236850616678 1107 72-73 -2.4673303064697336 1107 74-75 -0.3107963151913218 1107 76-77 -0.22000793058099788 1107 78-79 -0.07711996403739718 1107 80-81 -0.21517679747351437 1107 82-83 -0.11296183745820088 1107 84-85 -0.11997594059209504 1107 86-87 0.05057610577919647 1107 88-89 -0.03851431549708906 1107 90-91 0.008704499899142348 1107 92-93 0.14205716691882486 1107 94-95 0.982937778754188 1107 96-97 0.9559466635351477 1107 98-99 0.6788996866526169 1107 100-101 0.49558516670050423 1108 1 0.024628839156783044 1108 2 -0.040984372120359325 1108 3 0.058006183115210774 1108 4 0.00684023016138724 1108 5 -0.03294522016644663 1108 6 0.14737087177555708 1108 7 0.13454407929250323 1108 8 0.022332287178770116 1108 9 0.11015118501194365 1108 10-11 0.19810761968710722 1108 12-13 0.17321911583093197 1108 14-15 0.13430153718829985 1108 16-17 0.029813939099518905 1108 18-19 0.16558762268065408 1108 20-21 0.33790027866616157 1108 22-23 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2315 84-85 -4.5088717659218425 2315 86-87 -4.848923098435719 2315 88-89 -3.437258209904641 2315 90-91 -3.159571344822151 2315 92-93 -3.924165186409752 2315 94-95 -1.7969796242423577 2315 96-97 -0.5647387178014078 2315 98-99 -1.8460772172860622 2315 100-101 -1.0416255608482992 2316 1 -0.3174495805078408 2316 2 -0.29512367151827945 2316 3 -0.9319684814284166 2316 4 -0.37167052047580285 2316 5 -0.24240157904809223 2316 6 -2.223849542548308 2316 7 -0.31568342840627395 2316 8 -0.25079443709737603 2316 9 0.010878145365339265 2316 10-11 -0.03856310369337734 2316 12-13 -0.46980458035368855 2316 14-15 -0.8761878669792651 2316 16-17 -1.4463810291931551 2316 18-19 -0.2049206206724108 2316 20-21 -4.34844455997753 2316 22-23 -4.241359850755032 2316 24-25 -2.814568493610963 2316 26-27 -1.8443148120821817 2316 28-29 -1.2043012111146112 2316 30-31 -0.256373536663844 2316 32-33 -2.0044415118492154 2316 34-35 -2.50608652952684 2316 36-37 -4.674007407690631 2316 38-39 -2.37169548464869 2316 40-41 -1.5312573323580096 2316 42-43 -2.947483159410247 2316 44-45 -0.8038527456844875 2316 46-47 -0.7671497265684977 2316 48-49 -0.4409534728210218 2316 50-51 -0.1133033468341722 2316 52-53 -2.423863322027074 2316 54-55 -0.8960019274964637 2316 56-57 0.1893516439540157 2316 58-59 0.253783586427641 2316 60-61 -0.9404696359371947 2316 62-63 -0.5045218723841707 2316 64-65 0.3477833341963539 2316 66-67 1.6294508447874136 2316 68-69 4.0443468898673025 2316 70-71 4.011798102084942 2316 72-73 2.263701291886427 2316 74-75 1.2611791833192676 2316 76-77 0.1495734302284326 2316 78-79 0.43756531819174427 2316 80-81 0.02679070545890383 2316 82-83 0.279617358849066 2316 84-85 0.5189314077450753 2316 86-87 0.20454794615454475 2316 88-89 0.6716053650173279 2316 90-91 0.7566660493957791 2316 92-93 0.8112907517225842 2316 94-95 1.43957284284539 2316 96-97 1.4776982225674082 2316 98-99 0.6417102273140323 2316 100-101 0.5290442352472908 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 36.0 4 94.0 5 293.0 6 600.0 7 1427.0 8 3090.0 9 5055.0 10 8396.0 11 12349.0 12 16090.0 13 20207.0 14 25075.0 15 31675.0 16 37955.0 17 43198.0 18 37951.0 19 30883.0 20 29538.0 21 29997.0 22 31616.0 23 35817.0 24 43264.0 25 54191.0 26 68257.0 27 84541.0 28 102826.0 29 123796.0 30 145804.0 31 169771.0 32 197362.0 33 228746.0 34 267183.0 35 308207.0 36 326449.0 37 280892.0 38 123931.0 39 7823.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.912104063683856 20.713920992370834 15.543220910359533 21.830754033585777 2 15.79808274040305 21.775535767542927 39.24379873034809 23.182582761705937 3 18.964684665404167 26.354361092301602 30.756023992712628 23.9249302495816 4 12.726524856435287 18.159406841146936 36.920437664919234 32.19363063749855 5 12.761468490192845 38.932547434081656 33.79897273298555 14.50701134273995 6 30.481997797839483 37.486551724020416 17.26555867980784 14.765891798332259 7 27.307369740433362 34.6429974737518 20.418171947773832 17.63146083804101 8 26.715764872506764 35.00886555336354 19.337368469300785 18.938001104828906 9 26.726022537555494 16.743542356829746 20.564266997819992 35.96616810779477 10-11 24.752853317775305 27.70907149047281 26.635339325945317 20.90273586580657 12-13 25.95809488237934 25.405918979440706 27.64692912430552 20.98905701387443 14-15 22.728020907915816 26.302118565658088 26.92666042119131 24.04320010523478 16-17 20.961695158195205 29.798260689392414 27.57205011891776 21.66799403349463 18-19 20.729824869907816 27.25349279562181 31.378938825362862 20.637743509107516 20-21 21.86437385623588 26.276330003850884 31.504367176823806 20.35492896308943 22-23 21.367738655141732 26.564353557518412 31.305013544548082 20.762894242791774 24-25 21.003847135572595 26.864722526752573 31.543802444868458 20.587627892806378 26-27 20.896940727913226 27.089214413497665 31.442680544013722 20.571164314575388 28-29 20.513923446606906 27.08909642455435 31.718241930536145 20.6787381983026 30-31 20.79144602629213 26.96741122640037 31.511432857733908 20.7297098895736 32-33 20.608227047619568 27.009857365895616 31.30506916651529 21.076846419969524 34-35 21.181966577014528 27.0239767071439 31.211247943803222 20.582808772038348 36-37 21.20866392273977 26.807066211578018 31.116564245567073 20.86770562011514 38-39 20.87770103712457 27.240212269058812 31.1101551695553 20.77193152426132 40-41 21.056960123972544 27.04018104577271 31.206802932065358 20.69605589818939 42-43 20.94447341260318 27.09348960105643 30.855933187770468 21.106103798569922 44-45 20.95571693523546 27.39026452766766 30.593799750096114 21.060218787000768 46-47 21.179610535208358 27.57727356225148 29.96466466051586 21.278451242024303 48-49 21.22863929947597 27.840433539474496 29.59643420195534 21.334492959094195 50-51 21.23482673551035 27.981761381312868 29.44648627037884 21.336925612797938 52-53 21.558636312168584 28.060426150742064 29.15025640256089 21.23068113452847 54-55 21.168083337482294 27.90805132342721 29.332076596388973 21.591788742701524 56-57 21.354739647272076 28.24453543762352 28.975228999058743 21.425495916045666 58-59 21.49234095020446 27.766192135339846 29.12647550782691 21.614991406628782 60-61 21.49932534977351 27.956472705720603 29.36691599812277 21.177285946383115 62-63 21.466719671501796 27.80906740514466 29.420869921124922 21.303343002228623 64-65 21.22836981383756 27.757661826058104 29.33924764466147 21.674720715442866 66-67 20.900960276934256 27.973600768166797 29.55905692879743 21.566382026101518 68-69 21.135343758110096 27.55433967702005 29.911465027337513 21.39885153753234 70-71 20.574241971030037 27.624779527802705 30.264154057204156 21.536824443963102 72-73 21.29500690462123 27.827725172495928 29.185480235970257 21.69178768691259 74-75 20.95486647032635 28.045557232963265 29.17211653529817 21.827459761412214 76-77 21.05179294274993 28.128504753726318 28.995711088915105 21.823991214608647 78-79 21.133600837992685 28.079843784640516 28.804209185303637 21.982346192063165 80-81 20.86950268952788 28.432811558563003 28.792261575329952 21.90542417657916 82-83 21.156025219542997 28.364079633559395 28.496986257114337 21.982908889783264 84-85 21.017904566910136 28.250631902837757 28.420701263055946 22.310762267196157 86-87 21.009513066622137 28.073344158997543 28.631502001271137 22.285640773109186 88-89 21.097667742310353 28.102432313340493 28.41311260656898 22.386787337780177 90-91 20.91829397130491 28.245799679651025 28.557560576628156 22.278345772415907 92-93 21.019928896934633 28.038579138284287 28.594991977731215 22.346499987049864 94-95 20.542190874220584 27.858094509170282 28.863937764836027 22.73577685177311 96-97 20.526349943645616 27.7051811878821 28.892516820929675 22.875952047542608 98-99 20.62891521217619 27.654130205350157 28.436103277160086 23.28085130531357 100-101 20.527740203280587 27.61585810779263 28.70894368460461 23.147458004322175 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 5863.0 1 3923.0 2 2137.0 3 2401.0 4 2255.5 5 2269.5 6 3698.5 7 6812.0 8 9631.5 9 10819.5 10 9833.5 11 7919.0 12 7085.5 13 6708.0 14 6540.5 15 6474.5 16 6454.5 17 6543.0 18 6850.5 19 7309.0 20 7973.5 21 9004.0 22 10343.5 23 12012.5 24 13848.5 25 16528.5 26 20738.5 27 25030.0 28 28839.0 29 33344.0 30 38404.0 31 44555.0 32 51750.0 33 58830.0 34 66603.0 35 73361.0 36 80626.5 37 88302.0 38 92257.0 39 93543.0 40 93010.0 41 93248.5 42 94515.5 43 96026.0 44 97937.0 45 99727.5 46 100462.0 47 101132.5 48 105543.5 49 104973.5 50 98062.5 51 92120.5 52 88068.0 53 86342.0 54 84214.0 55 78851.0 56 71076.0 57 64070.5 58 58182.5 59 51516.0 60 44159.5 61 36477.5 62 28929.0 63 21742.0 64 15922.5 65 11993.0 66 8736.0 67 6560.0 68 4500.0 69 2768.5 70 2048.5 71 1652.0 72 1309.5 73 1115.5 74 927.5 75 726.5 76 516.0 77 281.5 78 152.5 79 102.0 80 71.0 81 53.0 82 33.0 83 18.5 84 12.5 85 5.5 86 3.0 87 2.5 88 2.0 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02211702530439877 2 0.017584568654961115 3 0.0 4 0.0 5 1.0223586427302974E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.407862142434325E-4 18-19 0.0010053193320181258 20-21 3.4078621424343246E-5 22-23 0.0 24-25 0.0 26-27 5.622972535016635E-4 28-29 0.016732603119352533 30-31 0.004396142163740279 32-33 0.008178869141842378 34-35 0.009286424338133535 36-37 0.10044673664825171 38-39 0.10281520083724356 40-41 0.11653184596054174 42-43 0.1026277684194097 44-45 0.09760117175931905 46-47 0.09904951316985365 48-49 0.10174172426237675 50-51 0.09947549593765793 52-53 0.07780149271177564 54-55 0.06573766072755811 56-57 0.03687306838113939 58-59 0.01511386860169623 60-61 0.009473856755967423 62-63 0.002300306946143169 64-65 0.005196989767212345 66-67 0.4126580268273724 68-69 0.7934014246908473 70-71 0.8813072286549407 72-73 0.27569604732293684 74-75 0.1456690672783552 76-77 0.014636767901755424 78-79 0.008059593966857178 80-81 0.0016016952069441324 82-83 0.0 84-85 0.0 86-87 2.044717285460595E-4 88-89 8.17886914184238E-4 90-91 0.003101154549615235 92-93 0.0020106386640362517 94-95 0.21810317711579677 96-97 0.22120433166541198 98-99 0.013716645123298158 100-101 0.007497296713355514 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2934391.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.09568349832466 #Duplication Level Percentage of deduplicated Percentage of total 1 68.45609295566226 28.81706022593451 2 14.876463452726686 12.524697941607531 3 6.196803297279377 7.82576010910942 4 3.2132254953625408 5.41051693846116 5 1.9123705368804655 4.025127242602064 6 1.216679416250435 3.073017097524283 7 0.7949857121011715 2.3425826845610813 8 0.5905058175940758 1.9886196801087719 9 0.4329218561509722 1.6401727292434707 >10 2.051942163947977 15.173983341839566 >50 0.15098814222636955 4.417159419501919 >100 0.09876286659259495 7.6882624217595605 >500 0.006541217604235289 1.8343596676931404 >1k 0.0013900087408785663 1.0481809389347545 >5k 8.176522005168038E-5 0.26035006804263894 >10k+ 2.452956601550411E-4 1.9301494930760266 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22151 0.7548755431706272 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19826 0.6756427483590292 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14100 0.4805085620832397 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7564 0.2577706924537323 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4901 0.16701932360070623 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4530 0.1543761550522749 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.7039310712171623E-5 0.0 12-13 0.0 0.0 0.0 8.519655356085811E-5 0.0 14-15 0.0 0.0 0.0 1.3631448569737298E-4 0.0 16-17 0.0 0.0 0.0 2.2151103925823108E-4 0.0 18-19 0.0 0.0 0.0 2.5558966068257437E-4 0.0 20-21 0.0 0.0 0.0 4.2598276780429057E-4 0.0 22-23 0.0 0.0 0.0 9.03083467745096E-4 0.0 24-25 0.0 0.0 0.0 0.0012268303712763568 0.0 26-27 0.0 0.0 0.0 0.0020958352175971095 0.0 28-29 0.0 0.0 0.0 0.0037656876673899287 0.0 30-31 0.0 0.0 0.0 0.0088263629489049 0.0 32-33 0.0 0.0 0.0 0.0175334507228246 0.0 34-35 0.0 0.0 0.0 0.03135233171039578 0.0 36-37 0.0 0.0 0.0 0.05471322669678308 0.0 38-39 0.0 0.0 0.0 0.08935414537462799 0.0 40-41 0.0 0.0 0.0 0.13004401935529383 0.0 42-43 0.0 0.0 0.0 0.16911515881830336 0.0 44-45 0.0 0.0 0.0 0.21900626058354186 0.0 46-47 0.0 0.0 0.0 0.2811827053722561 0.0 48-49 0.0 0.0 0.0 0.3489480440745627 0.0 50-51 0.0 0.0 0.0 0.42167182219411115 0.0 52-53 0.0 0.0 0.0 0.4928109444174277 0.0 54-55 0.0 0.0 0.0 0.5710554592077197 0.0 56-57 0.0 0.0 0.0 0.6615512384000632 0.0 58-59 0.0 0.0 0.0 0.7509053837746913 0.0 60-61 0.0 3.4078621424343246E-5 0.0 0.8447408678666204 0.0 62-63 0.0 3.4078621424343246E-5 0.0 0.9388489809299443 0.0 64-65 0.0 3.4078621424343246E-5 0.0 1.0384778306640117 0.0 66-67 3.4078621424343246E-5 3.4078621424343246E-5 0.0 1.1378681300481088 0.0 68-69 3.4078621424343246E-5 3.4078621424343246E-5 0.0 1.262681081014766 0.0 70-71 3.4078621424343246E-5 3.4078621424343246E-5 0.0 1.430876117054612 0.0 72-73 3.4078621424343246E-5 3.4078621424343246E-5 0.0 1.6138612747926229 0.0 74-75 3.4078621424343246E-5 3.4078621424343246E-5 0.0 1.8122840480358615 0.0 76-77 3.4078621424343246E-5 3.4078621424343246E-5 0.0 2.0337780479833807 0.0 78-79 3.4078621424343246E-5 3.4078621424343246E-5 0.0 2.2836765788880893 0.0 80-81 3.4078621424343246E-5 3.4078621424343246E-5 0.0 2.5619626014392765 0.0 82-83 3.4078621424343246E-5 3.4078621424343246E-5 0.0 2.8565552443420117 0.0 84-85 6.815724284868649E-5 3.4078621424343246E-5 0.0 3.143020136035041 0.0 86-87 6.815724284868649E-5 3.4078621424343246E-5 0.0 3.44234970731576 0.0 88-89 6.815724284868649E-5 3.4078621424343246E-5 0.0 3.771685504760613 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11485 0.0 47.864235 1 GTATCAA 19125 0.0 36.69761 1 ATCAACG 24910 0.0 27.883081 3 TATCAAC 25440 0.0 27.472136 2 TCAACGC 25320 0.0 27.45029 4 CAACGCA 25735 0.0 26.952398 5 AACGCAG 26440 0.0 26.215816 6 TACCGTA 130 1.022936E-5 25.511454 7 GTACATG 18665 0.0 24.881823 1 TACATGG 18940 0.0 23.866346 2 ACGCAGA 30135 0.0 22.938478 7 CGCAGAG 30215 0.0 22.830704 8 ACATGGG 18895 0.0 22.717567 3 CATTCCG 585 0.0 21.86696 9 GAGTACT 17880 0.0 21.689348 12-13 GCAGAGT 31370 0.0 21.280264 9 AGTACTT 18275 0.0 21.103882 12-13 GTACTTT 18600 0.0 20.722395 14-15 CATGGGA 12640 0.0 20.203295 4 GTATAGG 915 0.0 20.198898 1 >>END_MODULE