FastQCFastQC Report
Fri 27 May 2016
SRR939510_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939510_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2918085
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162800.5579001297083532No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116950.4007765366670265No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87570.30009406854152637No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA51350.1759715703963387No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29420.10081954432444565No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA100350.048.216661
GTATCAA178700.042.334981
TATCAAC231150.032.3194272
ATCAACG234200.032.1843873
TCAACGC238650.031.6319624
CAACGCA239400.031.3101225
AACGCAG246100.030.3519926
GTACATG168050.027.3526611
ACGCAGA273150.026.689267
CGCAGAG276100.026.246358
TACATGG170600.026.2283042
GCAGAGT270550.024.398289
ACATGGG178400.024.174283
GAGTACT153500.023.27044112-13
AGTACTT157650.022.26116612-13
AACGCGG3750.022.0027946
GTACTTT165850.021.64375514-15
CATGGGA118000.021.4190654
ATCGACG2307.854396E-820.9272693
AGAGTAC248900.020.835610-11
Produced by FastQC (version 0.11.5)