FastQCFastQC Report
Fri 27 May 2016
SRR939510_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939510_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2918085
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT222250.761629630391164No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT189900.6507692544939575No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139890.47938973676229446No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA76420.2618840780854567No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46630.15979657892076482No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45370.15547867865398027No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA115350.045.9680331
GTATCAA189600.036.5289571
ATCAACG249150.027.6356963
TCAACGC251400.027.3576454
TATCAAC253850.027.258152
CAACGCA255650.027.0249485
AACGCAG260650.026.5065356
GTACATG181400.024.336941
TACATGG181400.023.624712
ACGCAGA298200.023.0097547
CGCAGAG299850.022.8198838
ACATGGG186800.021.9990983
GAGTACT173900.021.86322812-13
GCAGAGT308750.021.317379
AGTACTT177850.020.9644912-13
GTACTTT181900.020.87561614-15
CATGGGA125150.019.7977244
AGAGTAC287050.019.55625710-11
CATTCCG5700.019.1339269
CAGAGTA305650.018.86256610-11
Produced by FastQC (version 0.11.5)