##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939510_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2918085 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.897728476038225 31.0 31.0 31.0 28.0 34.0 2 31.14005966241559 31.0 31.0 34.0 28.0 34.0 3 31.139761521682885 31.0 31.0 34.0 28.0 34.0 4 35.0626839177063 35.0 35.0 37.0 32.0 37.0 5 34.647694978042104 35.0 35.0 37.0 32.0 37.0 6 34.71311665013185 35.0 35.0 37.0 32.0 37.0 7 34.54776300210583 35.0 35.0 37.0 30.0 37.0 8 34.45141831029596 35.0 35.0 37.0 30.0 37.0 9 35.929419122472446 37.0 35.0 39.0 30.0 39.0 10-11 35.73650818944616 37.0 35.0 39.0 30.0 39.0 12-13 35.7179198001429 37.0 35.0 39.0 30.0 39.0 14-15 36.51154918379691 38.0 35.0 40.0 30.0 41.0 16-17 36.44837607540562 38.0 35.0 40.0 30.0 41.0 18-19 36.48829626278878 38.0 35.0 40.0 30.0 41.0 20-21 36.39231739308485 38.0 34.5 40.0 30.0 41.0 22-23 36.23010330405043 38.0 34.0 40.0 29.5 41.0 24-25 36.06370342193596 38.0 34.0 40.0 29.0 41.0 26-27 35.60224736428171 38.0 34.0 40.0 27.0 41.0 28-29 35.38548003228144 38.0 34.0 40.0 27.0 41.0 30-31 35.05248647657625 37.5 33.0 40.0 27.0 41.0 32-33 34.746408517915 37.0 33.0 40.0 25.5 41.0 34-35 34.946170862055084 37.0 33.0 40.0 26.5 41.0 36-37 34.749377588384164 37.0 33.0 40.0 25.0 41.0 38-39 34.54462687001921 37.0 33.0 40.0 25.0 41.0 40-41 34.35763574398963 37.0 33.0 40.0 24.5 41.0 42-43 34.15959027924136 37.0 33.0 40.0 24.0 41.0 44-45 33.72363519225794 37.0 32.5 40.0 23.5 41.0 46-47 33.25195427823384 37.0 32.0 40.0 21.0 41.0 48-49 32.816319264174965 36.5 31.0 39.5 18.5 41.0 50-51 32.56395118716556 36.0 31.0 39.0 18.0 40.5 52-53 32.45873372434319 36.0 31.0 39.0 18.0 40.0 54-55 32.12309117109337 36.0 30.5 39.0 15.0 40.0 56-57 31.63659506148724 35.5 29.5 39.0 12.5 40.0 58-59 31.18777982820925 35.0 29.0 39.0 9.0 40.0 60-61 30.831557168485496 35.0 28.0 38.0 8.0 40.0 62-63 30.680809160802376 34.5 28.0 38.0 8.0 40.0 64-65 30.24050687351465 34.0 28.0 38.0 6.5 40.0 66-67 29.403896390955026 34.0 27.0 37.5 2.0 39.5 68-69 26.045228805877827 31.0 17.0 36.0 2.0 39.0 70-71 24.775000899562556 29.5 12.5 35.0 2.0 38.0 72-73 25.762032634416062 31.0 19.0 35.0 2.0 38.0 74-75 26.482070604523173 31.0 21.5 35.0 2.0 37.5 76-77 26.133795622814276 30.5 22.5 34.0 2.0 36.0 78-79 27.21008897958764 32.0 25.5 35.0 2.0 37.0 80-81 27.22302623124412 32.0 26.0 35.0 2.0 36.5 82-83 26.898512894586688 32.0 25.0 35.0 2.0 36.0 84-85 26.53318580507422 32.0 24.5 35.0 2.0 36.0 86-87 26.0870978741195 32.0 24.0 34.0 2.0 35.0 88-89 25.603453977522932 31.5 22.0 34.0 2.0 35.0 90-91 25.014024779949864 31.0 20.0 34.0 2.0 35.0 92-93 24.461852550559698 31.0 18.0 34.0 2.0 35.0 94-95 23.62700589599001 30.5 7.5 34.0 2.0 35.0 96-97 22.970366867311952 30.0 2.0 34.0 2.0 35.0 98-99 22.14151815317237 30.0 2.0 34.0 2.0 35.0 100-101 20.754062510173625 28.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.2844079007492759 1101 2 0.20911661298498885 1101 3 0.2555395521793251 1101 4 0.28049193317234966 1101 5 0.389019747056075 1101 6 0.34235174415750436 1101 7 0.5839501480872897 1101 8 0.6480747278906875 1101 9 0.5718349535190725 1101 10-11 0.6011258767436587 1101 12-13 0.5803687838037845 1101 14-15 0.6756101447375329 1101 16-17 0.6505577456808069 1101 18-19 0.5312169029989136 1101 20-21 0.675897632342334 1101 22-23 0.6786447150228199 1101 24-25 0.5381941652803519 1101 26-27 0.6004231557045898 1101 28-29 0.6236279372700793 1101 30-31 0.4076068380763047 1101 32-33 0.7256201502616477 1101 34-35 0.7109655883073387 1101 36-37 0.870562359834004 1101 38-39 0.7036379242115558 1101 40-41 0.6459137846257903 1101 42-43 0.5008069442088185 1101 44-45 0.6544857924487388 1101 46-47 0.5388972099609717 1101 48-49 0.6435109470060283 1101 50-51 0.4818810293981741 1101 52-53 0.16581694727148744 1101 54-55 0.21552339493428718 1101 56-57 0.4306615391549933 1101 58-59 0.5187346333179477 1101 60-61 0.4014035696604701 1101 62-63 0.043737877062973496 1101 64-65 -0.013716200731259676 1101 66-67 -0.2209837878526244 1101 68-69 -7.002640033990861 1101 70-71 -7.784173988872755 1101 72-73 -4.912073850145351 1101 74-75 -2.997890248152121 1101 76-77 -0.45773349446811196 1101 78-79 -0.3126235267106523 1101 80-81 -0.41933076131708447 1101 82-83 -0.5167545010585961 1101 84-85 -0.5254877413694814 1101 86-87 -0.7050446544730633 1101 88-89 -0.5200719153128688 1101 90-91 -0.7309174677342938 1101 92-93 -0.6459743303337575 1101 94-95 -0.627201624978106 1101 96-97 -0.8374830760584757 1101 98-99 -0.98298851581416 1101 100-101 -0.7734853159068393 1102 1 0.33907701325383854 1102 2 0.2931385129775599 1102 3 0.37144334184482375 1102 4 0.2490926255074939 1102 5 0.3999238940022991 1102 6 0.33527181671484385 1102 7 0.5356887084747157 1102 8 0.5567584308088342 1102 9 0.5280030863862066 1102 10-11 0.5740553337397074 1102 12-13 0.5219100159222876 1102 14-15 0.6945035137490123 1102 16-17 0.5660300353725134 1102 18-19 0.46230898972682866 1102 20-21 0.5357989034317612 1102 22-23 0.5105942350014914 1102 24-25 0.4568974164134758 1102 26-27 0.6064464734842332 1102 28-29 0.48946214322530324 1102 30-31 0.45216956189545954 1102 32-33 0.7121433796826295 1102 34-35 0.5401210819387359 1102 36-37 0.7072355763098912 1102 38-39 0.7879539482216344 1102 40-41 0.7061189265128007 1102 42-43 0.4584669012177258 1102 44-45 0.5254368049094396 1102 46-47 0.7713364069558466 1102 48-49 0.8068954723580859 1102 50-51 0.7365967300944973 1102 52-53 0.4682927812284632 1102 54-55 0.13761367744516662 1102 56-57 0.4225531897777657 1102 58-59 0.514814312384722 1102 60-61 0.5977090845566444 1102 62-63 0.4142315094703015 1102 64-65 0.4025869943705338 1102 66-67 0.36969481880518273 1102 68-69 -6.609384532525606 1102 70-71 -7.628032798818236 1102 72-73 -1.5203316299077976 1102 74-75 0.14559490486018234 1102 76-77 0.5093325607660724 1102 78-79 0.46731285302956493 1102 80-81 0.18604635405301195 1102 82-83 -0.03227490725337745 1102 84-85 -0.16033044574771793 1102 86-87 -0.19478007038527423 1102 88-89 -0.2170265070584101 1102 90-91 -0.3186259379999363 1102 92-93 -0.35135497802601634 1102 94-95 -0.1623588578480124 1102 96-97 -0.3543582644405596 1102 98-99 -0.46871707396073603 1102 100-101 -0.3059617794066156 1103 1 0.41852632612928176 1103 2 0.31975621983765734 1103 3 0.39405441810795594 1103 4 0.43730189116035945 1103 5 0.47800974359871873 1103 6 0.5374003006461479 1103 7 0.5406489208763929 1103 8 0.4895283665121042 1103 9 0.534273077782025 1103 10-11 0.589851444744312 1103 12-13 0.5627906968192775 1103 14-15 0.7076773727546808 1103 16-17 0.7418575791136988 1103 18-19 0.7173467651441072 1103 20-21 0.5357382147192098 1103 22-23 0.6140843600809731 1103 24-25 0.7033118106984944 1103 26-27 0.7646446074851099 1103 28-29 0.718862243873545 1103 30-31 0.6282640086186646 1103 32-33 0.7299763586637624 1103 34-35 0.7985546332844748 1103 36-37 1.0270976155348848 1103 38-39 1.190587358322837 1103 40-41 1.0333420314452937 1103 42-43 0.7677544098544828 1103 44-45 0.8600306250057557 1103 46-47 0.7579875842042156 1103 48-49 0.7153538722783779 1103 50-51 0.6825367531885362 1103 52-53 0.6130552963528899 1103 54-55 0.4936928202286097 1103 56-57 0.8132287193149459 1103 58-59 0.9527266735733662 1103 60-61 0.7931169915660625 1103 62-63 0.4495928483015028 1103 64-65 0.4339718755240085 1103 66-67 0.49437181917421213 1103 68-69 -6.731174729050252 1103 70-71 -7.41035424052734 1103 72-73 -0.735659198580823 1103 74-75 0.7160680191727344 1103 76-77 0.9033340178829583 1103 78-79 0.7641966468313619 1103 80-81 0.5736974892748101 1103 82-83 0.451588066940257 1103 84-85 0.3259934336981445 1103 86-87 0.37038951660525754 1103 88-89 0.4369996820558484 1103 90-91 0.12584344917338086 1103 92-93 0.09120430661717194 1103 94-95 0.4217169529830649 1103 96-97 0.43834932303959917 1103 98-99 0.5527128990531125 1103 100-101 0.6672119483272638 1104 1 0.2785864306569472 1104 2 0.27031567801155987 1104 3 0.18550489978185425 1104 4 0.20809532093321792 1104 5 0.36922393425260225 1104 6 0.21966442530257524 1104 7 0.28472065481000186 1104 8 0.4459214021390565 1104 9 0.47007356554149027 1104 10-11 0.5059180276951807 1104 12-13 0.3915422168719047 1104 14-15 0.5880971101327788 1104 16-17 0.4981480601376944 1104 18-19 0.41263632647484627 1104 20-21 0.4594944957143454 1104 22-23 0.3205402214691091 1104 24-25 0.4242981055858337 1104 26-27 0.5578923486034739 1104 28-29 0.44732740687951633 1104 30-31 0.396238913728169 1104 32-33 0.46878863023021466 1104 34-35 0.32748440045604355 1104 36-37 0.6663990864700722 1104 38-39 0.662459027013 1104 40-41 0.6940799536974112 1104 42-43 0.5164825955973029 1104 44-45 0.621202543627156 1104 46-47 0.6083039798939396 1104 48-49 0.7082083722908834 1104 50-51 0.650711552698219 1104 52-53 0.4393460799523652 1104 54-55 0.5700260957385765 1104 56-57 0.5067032169204673 1104 58-59 0.5627240496310151 1104 60-61 0.7806894090186525 1104 62-63 0.2954972848461246 1104 64-65 0.34851614382498397 1104 66-67 0.29669346819696685 1104 68-69 -6.575210591736248 1104 70-71 -4.147872783004704 1104 72-73 0.4979804916169073 1104 74-75 1.0307947278412897 1104 76-77 1.1374793301958235 1104 78-79 0.6334450736577608 1104 80-81 0.2697892161509188 1104 82-83 0.26207813511897626 1104 84-85 0.13637234673610266 1104 86-87 0.15956231512783603 1104 88-89 0.25645751349250645 1104 90-91 0.11687704799156862 1104 92-93 0.18935239873282228 1104 94-95 0.4466749061795987 1104 96-97 0.25447464065872794 1104 98-99 0.23352539108131865 1104 100-101 0.48595337636873026 1105 1 0.1788174988494582 1105 2 0.2607382285201041 1105 3 0.18759874692086598 1105 4 0.22298589908491095 1105 5 0.39195283868711783 1105 6 0.2874042309030429 1105 7 0.31720113363995495 1105 8 0.47172213270994945 1105 9 0.3944754315188419 1105 10-11 0.3829212975135121 1105 12-13 0.33005549553852376 1105 14-15 0.3230876062177259 1105 16-17 0.3313672749851193 1105 18-19 0.44584804293572944 1105 20-21 0.44802491147958534 1105 22-23 0.45685137088383243 1105 24-25 0.4015981897590919 1105 26-27 0.6413084202548092 1105 28-29 0.5910366905681244 1105 30-31 0.5207059944101289 1105 32-33 0.5389031342646078 1105 34-35 0.5665023804467566 1105 36-37 0.6842204128670133 1105 38-39 0.5936148520853024 1105 40-41 0.5693185903322586 1105 42-43 0.4847212758116939 1105 44-45 0.5733938552969207 1105 46-47 0.5936733812395758 1105 48-49 0.582088361194046 1105 50-51 0.5586119919046197 1105 52-53 0.4649893540687273 1105 54-55 0.5995991138381882 1105 56-57 0.6456142579371225 1105 58-59 0.8845808414637233 1105 60-61 0.9660625209060782 1105 62-63 0.7117148938213056 1105 64-65 0.5462035679775035 1105 66-67 0.7088360036352981 1105 68-69 -2.9994698337633885 1105 70-71 -3.065673459850043 1105 72-73 1.049977115796903 1105 74-75 1.3578955105047115 1105 76-77 1.2559449960404692 1105 78-79 0.902262279517366 1105 80-81 0.6057928350887103 1105 82-83 0.6463165749211832 1105 84-85 0.7304007128618295 1105 86-87 0.7931765577333003 1105 88-89 0.8729887695408607 1105 90-91 0.8023020494868938 1105 92-93 0.7167534937098416 1105 94-95 0.7864292644476798 1105 96-97 0.6349300188329927 1105 98-99 0.6032579798712518 1105 100-101 0.806671688945741 1106 1 0.033554953636802054 1106 2 7.889750572331877E-5 1106 3 -0.004116770201047615 1106 4 0.12936452129419962 1106 5 0.3102074338635106 1106 6 0.150426630511312 1106 7 0.09963356295115489 1106 8 0.16545662875341094 1106 9 0.10430921293673379 1106 10-11 0.23181719638498777 1106 12-13 0.10609304802677144 1106 14-15 0.1580354297740385 1106 16-17 0.14249559346698248 1106 18-19 0.13380161306096028 1106 20-21 0.19877513552186343 1106 22-23 0.21169801740979466 1106 24-25 0.24776487259375557 1106 26-27 0.4423287076294429 1106 28-29 0.21130018853788357 1106 30-31 0.26260452751064634 1106 32-33 0.3966995167902354 1106 34-35 0.24465049202442657 1106 36-37 0.271848948462015 1106 38-39 0.31090583449914533 1106 40-41 0.23603145128284808 1106 42-43 0.14585170190776608 1106 44-45 0.17811543079916703 1106 46-47 0.32966337671253854 1106 48-49 0.5869184066492181 1106 50-51 0.3492838935385052 1106 52-53 0.2210690992610651 1106 54-55 0.11182775129159239 1106 56-57 0.27931538438473424 1106 58-59 0.4224483169923019 1106 60-61 0.7024059026510194 1106 62-63 0.1527943733964996 1106 64-65 0.36667828931048163 1106 66-67 0.27648450093524346 1106 68-69 -3.15343177313801 1106 70-71 -3.1576384838479754 1106 72-73 0.9487346916594674 1106 74-75 1.2047709868832648 1106 76-77 1.1037241480981947 1106 78-79 0.7939997677955652 1106 80-81 0.6093487844505354 1106 82-83 0.39815741713536923 1106 84-85 0.4119695111637327 1106 86-87 0.40998096998032807 1106 88-89 0.2606126568360452 1106 90-91 0.5097331507124316 1106 92-93 0.4949885948984196 1106 94-95 0.68026368450154 1106 96-97 0.557293884278284 1106 98-99 0.6584862777966656 1106 100-101 0.8500995126868283 1107 1 -0.09110990650054873 1107 2 0.0369729574652915 1107 3 0.011162262110978816 1107 4 -0.09780235804588955 1107 5 0.19399216056621071 1107 6 0.10112701905848809 1107 7 0.02087689002376436 1107 8 0.039112783617895275 1107 9 0.24191160682703128 1107 10-11 -0.05029858159351619 1107 12-13 0.07315659556287102 1107 14-15 0.03288070388380504 1107 16-17 -0.050048530294610316 1107 18-19 0.1606743339904142 1107 20-21 0.2159633001263117 1107 22-23 0.3244546592725257 1107 24-25 0.199279513605795 1107 26-27 0.4573778913458284 1107 28-29 0.21355521384362675 1107 30-31 0.27597621972201836 1107 32-33 0.2863724895823765 1107 34-35 0.07253989348016177 1107 36-37 0.24898386704872166 1107 38-39 0.45522745406700693 1107 40-41 0.3181401202339629 1107 42-43 0.18392308251673484 1107 44-45 0.22922127286644667 1107 46-47 0.3586095141808201 1107 48-49 0.3992963882589393 1107 50-51 0.1446721957832935 1107 52-53 -0.15365370841047366 1107 54-55 0.023024200601234668 1107 56-57 0.3481362954394527 1107 58-59 0.6812279658182945 1107 60-61 0.4283354229171259 1107 62-63 0.1227246810321958 1107 64-65 0.16604227031802665 1107 66-67 0.2354816919733267 1107 68-69 -2.8680319530740626 1107 70-71 -3.1611484914468697 1107 72-73 0.6457540676421836 1107 74-75 1.0410754754873146 1107 76-77 0.7351303376117215 1107 78-79 0.5905619556554953 1107 80-81 0.36770084618795806 1107 82-83 0.3973652683691107 1107 84-85 0.15299496148178449 1107 86-87 -0.046359296745599465 1107 88-89 0.1274263728381655 1107 90-91 0.16705025156742437 1107 92-93 0.10089996026180614 1107 94-95 0.3681823106312194 1107 96-97 0.4452469503599765 1107 98-99 0.5794098479860637 1107 100-101 0.8248529070980588 1108 1 -0.06304274402482335 1108 2 0.0010966802565164357 1108 3 0.007987574656620922 1108 4 -0.040147669973094935 1108 5 -0.14343761914337705 1108 6 -0.2014385670080756 1108 7 0.1170978615344751 1108 8 0.20209404206802617 1108 9 0.11593904029296453 1108 10-11 0.14270707671649063 1108 12-13 0.0062975923319399385 1108 14-15 0.0930040112630337 1108 16-17 0.17137913004079053 1108 18-19 0.2315759244771911 1108 20-21 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5.1641972954892275 2311 72-73 3.9849924690018703 2311 74-75 2.8999652126517965 2311 76-77 2.751641334243839 2311 78-79 2.091588865861322 2311 80-81 1.8001196467262695 2311 82-83 1.5584419135079237 2311 84-85 1.487014557412202 2311 86-87 1.3937075488995774 2311 88-89 1.423627015234981 2311 90-91 1.6051280437888131 2311 92-93 1.9261947360304674 2311 94-95 2.305569063928985 2311 96-97 2.256091809924513 2311 98-99 2.708571843405373 2311 100-101 2.8143222533985472 2312 1 -0.07846565523934501 2312 2 -0.024541378597206176 2312 3 0.024269856269253154 2312 4 0.15371503692795585 2312 5 -1.209950468676773 2312 6 -0.06162363142404104 2312 7 0.12657460278509802 2312 8 0.2267128476588809 2312 9 -2.7823254943216753 2312 10-11 -0.1226635044156481 2312 12-13 -1.090530415780016 2312 14-15 -4.379198722313205 2312 16-17 -1.0610232362256156 2312 18-19 -0.022382491251910608 2312 20-21 0.2819478481178237 2312 22-23 0.3682270444599709 2312 24-25 0.4222031118609948 2312 26-27 0.6097469969774068 2312 28-29 0.4341779355643425 2312 30-31 0.1505550892713785 2312 32-33 0.5651484558696751 2312 34-35 0.39006271073221654 2312 36-37 0.6729179000616412 2312 38-39 0.542451953243166 2312 40-41 0.4673891495464986 2312 42-43 -0.035993624440621375 2312 44-45 0.6538833214782187 2312 46-47 0.8537235394748848 2312 48-49 0.9286596178119524 2312 50-51 1.0130871866754774 2312 52-53 0.951406443994351 2312 54-55 1.0967499943807795 2312 56-57 1.111701467215621 2312 58-59 1.0133658320659364 2312 60-61 1.0113290023841444 2312 62-63 1.2143213823912653 2312 64-65 1.2335535570117138 2312 66-67 1.7382763282226463 2312 68-69 4.302947341223955 2312 70-71 5.055766758153936 2312 72-73 3.3127960075963365 2312 74-75 2.587117326377843 2312 76-77 2.539004287138095 2312 78-79 1.9470229383665512 2312 80-81 1.7009993890434636 2312 82-83 1.608165942389892 2312 84-85 1.687673977243925 2312 86-87 1.5922669737363826 2312 88-89 1.5330388124816565 2312 90-91 1.5979757127367442 2312 92-93 1.879152213889558 2312 94-95 1.9344223201313397 2312 96-97 2.200743647053809 2312 98-99 2.243933421218628 2312 100-101 2.137826852807418 2313 1 -0.24322944122059198 2313 2 -0.14155796767333229 2313 3 -0.08166368907203747 2313 4 0.1276205185357142 2313 5 0.0711709331985162 2313 6 -0.07278306359242492 2313 7 -2.755651644674085 2313 8 -2.840663389964881 2313 9 -0.9057024859943539 2313 10-11 -0.015936978286212877 2313 12-13 0.07842932456487972 2313 14-15 -0.541936985377923 2313 16-17 -0.19599635274256144 2313 18-19 0.13394914273930425 2313 20-21 -2.500017842656554 2313 22-23 -0.5569231096282863 2313 24-25 0.07653069693738246 2313 26-27 -1.383249773726149 2313 28-29 -1.6929292213434053 2313 30-31 -0.8430655749473601 2313 32-33 -0.4815521703187784 2313 34-35 -1.0796738639186287 2313 36-37 -1.9525785427945692 2313 38-39 -1.6453869589593069 2313 40-41 -0.7608494905426753 2313 42-43 0.061262211242286924 2313 44-45 0.13303526233455187 2313 46-47 -0.14905029502777012 2313 48-49 -2.2698430213592538 2313 50-51 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0.8220274540380537 2314 74-75 0.4368013114509033 2314 76-77 0.5730471758167432 2314 78-79 -1.6933980644716726 2314 80-81 -1.3173169938010645 2314 82-83 -0.147963170403127 2314 84-85 -0.03604488116036464 2314 86-87 -0.4606021264423177 2314 88-89 -0.4550012379756154 2314 90-91 -0.07548954533116259 2314 92-93 -0.49062204239132257 2314 94-95 0.11301214458208975 2314 96-97 0.40968075942635096 2314 98-99 0.09266804751948143 2314 100-101 0.17431770742741293 2315 1 -0.40118430553293294 2315 2 -0.4314990427525025 2315 3 -0.4264081473235315 2315 4 -0.30391593875373957 2315 5 -0.3034248097765939 2315 6 -0.21760769180423267 2315 7 -1.253585664796745 2315 8 -2.810837759628569 2315 9 -0.7698657120335568 2315 10-11 -1.4388479495452842 2315 12-13 -0.7946359738677984 2315 14-15 -0.5037608519751089 2315 16-17 -0.33621762183302906 2315 18-19 -0.22629617733926466 2315 20-21 -0.7329985617810948 2315 22-23 -0.371136041042881 2315 24-25 -4.971801767888049 2315 26-27 -0.8783453764038285 2315 28-29 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-1.1519669109730302 2315 94-95 -0.18608335056603664 2315 96-97 -0.31165061387870807 2315 98-99 -2.6115757350137763 2315 100-101 -4.316736852048198 2316 1 -0.5047203790467698 2316 2 -0.42146415547521343 2316 3 -0.9715905409121497 2316 4 -0.417778922816872 2316 5 -0.41200735646143727 2316 6 -0.4991010193316612 2316 7 -0.6894913948462857 2316 8 -1.2023844634806835 2316 9 -5.278821149973428 2316 10-11 -1.523133641614514 2316 12-13 -0.7529506021601904 2316 14-15 -0.5169984915285397 2316 16-17 -0.4208950397943667 2316 18-19 -0.5553239817562527 2316 20-21 -3.826096704063822 2316 22-23 -1.3937506515374096 2316 24-25 -0.5897353662886573 2316 26-27 -0.6987216025991785 2316 28-29 -0.364776096171731 2316 30-31 -0.31075606230595554 2316 32-33 -1.6749886970700416 2316 34-35 -0.8812483030526792 2316 36-37 -0.5944601112330972 2316 38-39 -5.4958765149571605 2316 40-41 -5.161882946000901 2316 42-43 -3.9758973075264947 2316 44-45 -2.6692661790771908 2316 46-47 -2.8235376874289813 2316 48-49 -3.1588937855906067 2316 50-51 -1.6785158481499742 2316 52-53 -1.2195152708219439 2316 54-55 -0.5372442303495006 2316 56-57 -4.309535933609158 2316 58-59 -1.3893915712744302 2316 60-61 -0.9748872490679759 2316 62-63 -0.8027165642328917 2316 64-65 -0.4508579491514979 2316 66-67 -0.5273108230211605 2316 68-69 2.3191889182195418 2316 70-71 3.3121910655879425 2316 72-73 2.291820200415305 2316 74-75 0.8701664009779613 2316 76-77 -1.548664914909434 2316 78-79 -0.2780179047253135 2316 80-81 -0.2396586193117045 2316 82-83 0.05940782323547111 2316 84-85 0.12898164161263992 2316 86-87 0.1389149253634656 2316 88-89 -0.17919793329906852 2316 90-91 -0.019837704996223948 2316 92-93 -0.08520900863321756 2316 94-95 0.03590963050774221 2316 96-97 0.1690203830265382 2316 98-99 0.18412055041564201 2316 100-101 0.22805528440443013 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 41.0 4 117.0 5 301.0 6 697.0 7 1588.0 8 3328.0 9 5744.0 10 9225.0 11 13135.0 12 17342.0 13 21734.0 14 26669.0 15 33628.0 16 40388.0 17 44034.0 18 39569.0 19 33576.0 20 32765.0 21 33321.0 22 35235.0 23 39746.0 24 46753.0 25 57275.0 26 71446.0 27 88526.0 28 107553.0 29 128331.0 30 150627.0 31 174892.0 32 202204.0 33 233427.0 34 267975.0 35 298456.0 36 300977.0 37 247281.0 38 104008.0 39 6164.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.715615622306125 20.85761929326357 15.633788575473137 21.79297650895717 2 15.791569882124227 21.9025604588487 39.17533228931132 23.130537369715753 3 18.92261998390037 26.41806476109797 30.81784283427556 23.841472420726102 4 12.678895148911357 18.237863703017673 37.00174080076835 32.081500347302615 5 12.695175089142365 39.01572435347154 33.79336105699457 14.495739500391524 6 30.383419262975547 37.5690221498003 17.31683621279024 14.730722374433919 7 27.21634222443829 34.643027876158506 20.48291944888514 17.657710450518064 8 26.688016284652434 35.004395005628695 19.377674056787242 18.929914652931632 9 26.726397620357183 16.750882856393833 20.56674154454034 35.955977978708646 10-11 24.717896763984694 27.790448220653406 26.61112311609155 20.88053189927035 12-13 25.81444680329737 25.451280548716028 27.69290476459733 21.041367883389277 14-15 22.665515226595524 26.307201469456853 27.000241596800638 24.02704170714698 16-17 20.94210883527317 29.84737688825544 27.5497627627571 21.660751513714285 18-19 20.651441597597326 27.34184645942095 31.400985304730984 20.60572663825074 20-21 21.82554654850698 26.302420937018628 31.529119268287246 20.342913246187138 22-23 21.360767572935778 26.59038594199407 31.33139553014464 20.717450954925514 24-25 20.91818209390357 26.883562377365156 31.630112323277743 20.568143205453534 26-27 20.892643444336457 27.100328444590055 31.47852156068754 20.52850655038595 28-29 20.60609871777649 27.06353133730127 31.685255526281882 20.645114418640357 30-31 20.784561202463127 26.98343806554796 31.53741316627354 20.694587565715366 32-33 20.69303128837625 27.032987104980034 31.26209170496403 21.01188990167969 34-35 21.135124854138358 27.05972059677759 31.209593540792426 20.595561008291625 36-37 21.165030329024166 26.844997773190588 31.137861024798784 20.852110872986458 38-39 20.880731395876335 27.24729203952666 31.14198708685969 20.72998947773731 40-41 21.029701360780788 26.998953298310667 31.254059741562084 20.717285599346464 42-43 20.883256662760257 27.105302599254184 30.94222663965448 21.06921409833108 44-45 20.949940341065957 27.38442817794859 30.63001823550171 21.035613245483738 46-47 21.23334840073997 27.58115650462688 29.974439139223524 21.211055955409623 48-49 21.22803380534406 27.80763389512675 29.65370047526869 21.310631824260497 50-51 21.225308377888666 27.989465502332262 29.49216951563019 21.29305660414888 52-53 21.474095928736908 28.09409668651931 29.20217342964599 21.229633955097793 54-55 21.13258962903128 27.894568480875904 29.401548551580042 21.571293338512774 56-57 21.351890726056904 28.215344915764344 29.00990027738053 21.42286408079822 58-59 21.485287489095654 27.80790528601519 29.13217694431543 21.57463028057373 60-61 21.44536553048284 27.93710848749919 29.447772228107777 21.169753753910197 62-63 21.41940179944058 27.815524098891782 29.44606578849068 21.31900831317696 64-65 21.17193648763481 27.795116228738692 29.31645606060684 21.71649122301966 66-67 21.05004023448407 28.049381758410842 29.26080955317156 21.639768453933534 68-69 21.444593508323667 27.54586015246393 29.60875161052841 21.400794728683987 70-71 20.845869284803047 27.581518756698635 30.089930454312043 21.482681504186274 72-73 21.22650796530328 27.793986279852156 29.275827589379137 21.70367816546543 74-75 21.155906033410982 28.04478684571987 28.90915424721055 21.890152873658593 76-77 20.493133075061827 28.18707962638874 29.46429092592154 21.85549637262789 78-79 21.091734786557677 28.077596695971074 28.827669528558943 22.00299898891231 80-81 20.842172180165115 28.439576557836045 28.782381022553196 21.935870239445645 82-83 21.106539539977945 28.387571906290333 28.530030835829876 21.975857717901846 84-85 20.95855559721159 28.191398584959003 28.489540032857242 22.360505784972162 86-87 20.988051172932945 28.07840708807698 28.63463909004269 22.29890264894739 88-89 21.10409754648226 28.036903089039335 28.47383489563584 22.385164468842564 90-91 20.912604407005905 28.216181297427063 28.57559242863397 22.295621866933065 92-93 20.99626486778989 28.02505783988795 28.592061807373554 22.386615484948607 94-95 20.759077961902577 27.79801475822024 28.658359359044727 22.784547920832452 96-97 20.81531046415141 27.669174427320563 28.621210440891304 22.89430466763672 98-99 20.601340921055517 27.66355713549321 28.406900513281713 23.32820143016956 100-101 20.553307392729387 27.54724540741292 28.771346931529074 23.12810026832862 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 5755.0 1 3900.5 2 2156.0 3 2429.5 4 2332.5 5 2296.0 6 3678.0 7 6860.0 8 9835.5 9 10929.0 10 9772.5 11 7750.5 12 6768.5 13 6425.5 14 6339.0 15 6400.0 16 6462.0 17 6608.0 18 6943.5 19 7409.5 20 7994.0 21 8936.0 22 10265.5 23 11841.5 24 13764.5 25 16509.5 26 20675.5 27 25339.5 28 29270.0 29 33603.0 30 38963.0 31 44835.5 32 51310.5 33 58170.5 34 65829.5 35 72724.0 36 80203.0 37 87810.5 38 91834.5 39 92848.0 40 92273.5 41 92857.5 42 94003.5 43 95097.0 44 97121.5 45 99237.5 46 99788.0 47 100164.0 48 104642.5 49 104210.5 50 97195.0 51 91469.0 52 87159.5 53 85491.0 54 83703.0 55 78370.5 56 70867.5 57 63418.0 58 57641.0 59 51350.5 60 43887.0 61 36473.0 62 29086.5 63 21883.5 64 15928.0 65 11911.0 66 8740.5 67 6544.0 68 4458.5 69 2882.5 70 2175.5 71 1663.5 72 1348.0 73 1196.0 74 943.0 75 710.5 76 510.5 77 302.0 78 180.0 79 106.0 80 73.0 81 56.5 82 31.0 83 15.5 84 9.0 85 5.5 86 1.0 87 0.5 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02021873934446735 2 0.017888443962393145 3 2.0561429841831202E-4 4 0.09458257727242353 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.010777616142093188 12-13 0.0 14-15 0.0 16-17 4.969012211775873E-4 18-19 1.0280714920915601E-4 20-21 0.0 22-23 1.3707619894554133E-4 24-25 0.0042322276424435895 26-27 0.003049945426538295 28-29 9.938024423551746E-4 30-31 2.7415239789108266E-4 32-33 7.025155195958994E-4 34-35 0.002741523978910827 36-37 0.08493583977163105 38-39 0.09723842862699339 40-41 0.07741378335449448 42-43 0.08101203357681493 44-45 0.08032665258208722 46-47 0.07878454534394988 48-49 0.07386693670677859 50-51 0.0733871700104692 52-53 0.057589138081995554 54-55 0.04879912682461272 56-57 0.027175356440953573 58-59 0.022960263323378173 60-61 0.013502005596135824 62-63 0.0022960263323378175 64-65 0.0067338682731997185 66-67 0.15556435127832124 68-69 0.7108771677315774 70-71 0.8648994117717613 72-73 0.35357092065515566 74-75 0.053425448539024735 76-77 0.1934830548116316 78-79 0.01490703663532762 80-81 0.0032555597249566068 82-83 0.001747721536555652 84-85 2.3988334815469733E-4 86-87 0.0019876048847103493 88-89 3.2555597249566066E-4 90-91 0.004026613344025277 92-93 0.0039752097694206985 94-95 0.07602588684017086 96-97 0.03678782489200966 98-99 0.013330660347453895 100-101 0.013502005596135824 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2918085.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.89485684332011 #Duplication Level Percentage of deduplicated Percentage of total 1 69.0927367429237 29.63723051500916 2 14.562928049090603 12.493494277706251 3 6.122947232439882 7.879288349841356 4 3.1480984188043397 5.401489240131783 5 1.8480681297608395 3.9636308931396775 6 1.2102112802450322 3.1147103769769084 7 0.8239966271187971 2.4741652151747595 8 0.5670993972875314 1.9460517970065423 9 0.41817763828398186 1.6143902936342194 >10 1.958104020390564 14.794960499727322 >50 0.1463306973691802 4.363327373671142 >100 0.0940008658826752 7.490772910283459 >500 0.006002962553446515 1.7833353030377426 >1k 9.734533870383108E-4 0.8563141165310002 >5k 8.112111558652589E-5 0.26591704167296126 >10k+ 2.433633467595777E-4 1.9209217964556597 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22225 0.761629630391164 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18990 0.6507692544939575 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13989 0.47938973676229446 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7642 0.2618840780854567 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4663 0.15979657892076482 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4537 0.15547867865398027 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 3.426904973638533E-5 0.0 22-23 0.0 0.0 0.0 2.3988334815469733E-4 0.0 24-25 0.0 0.0 0.0 7.539190942004774E-4 0.0 26-27 0.0 0.0 0.0 0.0015078381884009546 0.0 28-29 0.0 0.0 0.0 0.0032555597249566068 0.0 30-31 0.0 0.0 0.0 0.008053226688050554 0.0 32-33 0.0 0.0 0.0 0.016808968895697007 0.0 34-35 0.0 0.0 0.0 0.03091068286221957 0.0 36-37 0.0 0.0 0.0 0.05383667713586136 0.0 38-39 0.0 0.0 0.0 0.08875683881723802 0.0 40-41 0.0 0.0 0.0 0.12924572108077728 0.0 42-43 0.0 0.0 0.0 0.16922056759827078 0.0 44-45 0.0 0.0 0.0 0.21772840750012423 0.0 46-47 0.0 0.0 0.0 0.2773222849916983 0.0 48-49 0.0 3.426904973638533E-5 0.0 0.34428400817659527 0.0 50-51 0.0 3.426904973638533E-5 0.0 0.4164888959711591 0.0 52-53 0.0 3.426904973638533E-5 0.0 0.4884367658927002 0.0 54-55 0.0 3.426904973638533E-5 0.0 0.5691917815964922 0.0 56-57 0.0 3.426904973638533E-5 0.0 0.6645282779631162 0.0 58-59 0.0 3.426904973638533E-5 0.0 0.7592479314344853 0.0 60-61 0.0 3.426904973638533E-5 0.0 0.8585082339959254 0.0 62-63 0.0 3.426904973638533E-5 0.0 0.9571516936621106 0.0 64-65 0.0 3.426904973638533E-5 0.0 1.0604043405178396 0.0 66-67 3.426904973638533E-5 3.426904973638533E-5 0.0 1.1751199845103895 0.0 68-69 3.426904973638533E-5 3.426904973638533E-5 0.0 1.306079158077986 0.0 70-71 3.426904973638533E-5 3.426904973638533E-5 0.0 1.4680175526072752 0.0 72-73 3.426904973638533E-5 3.426904973638533E-5 0.0 1.6501061483815584 0.0 74-75 3.426904973638533E-5 3.426904973638533E-5 0.0 1.844120373464104 0.0 76-77 3.426904973638533E-5 3.426904973638533E-5 0.0 2.065309954987603 0.0 78-79 3.426904973638533E-5 3.426904973638533E-5 0.0 2.31657062765478 0.0 80-81 3.426904973638533E-5 3.426904973638533E-5 0.0 2.5966687056751256 0.0 82-83 3.426904973638533E-5 3.426904973638533E-5 0.0 2.885436853278777 0.0 84-85 3.426904973638533E-5 3.426904973638533E-5 0.0 3.179413896442359 0.0 86-87 3.426904973638533E-5 3.426904973638533E-5 0.0 3.488263707191531 0.0 88-89 3.426904973638533E-5 3.426904973638533E-5 0.0 3.822883843342466 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11535 0.0 45.968033 1 GTATCAA 18960 0.0 36.528957 1 ATCAACG 24915 0.0 27.635696 3 TCAACGC 25140 0.0 27.357645 4 TATCAAC 25385 0.0 27.25815 2 CAACGCA 25565 0.0 27.024948 5 AACGCAG 26065 0.0 26.506535 6 GTACATG 18140 0.0 24.33694 1 TACATGG 18140 0.0 23.62471 2 ACGCAGA 29820 0.0 23.009754 7 CGCAGAG 29985 0.0 22.819883 8 ACATGGG 18680 0.0 21.999098 3 GAGTACT 17390 0.0 21.863228 12-13 GCAGAGT 30875 0.0 21.31737 9 AGTACTT 17785 0.0 20.96449 12-13 GTACTTT 18190 0.0 20.875616 14-15 CATGGGA 12515 0.0 19.797724 4 AGAGTAC 28705 0.0 19.556257 10-11 CATTCCG 570 0.0 19.133926 9 CAGAGTA 30565 0.0 18.862566 10-11 >>END_MODULE