FastQCFastQC Report
Fri 27 May 2016
SRR939509_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939509_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences458250
Sequences flagged as poor quality0
Sequence length101
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82141.7924713584288055No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62151.3562465902891434No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA56861.2408074195308239No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47271.031533006001091No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15480.33780687397708675No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15400.33606110201854883No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14810.3231860338243317No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13320.290671031096563No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9980.21778505182760505No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7630.16650300054555373No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27300.053.2506521
GTATCAA49800.043.8369141
GGGATCG756.2601375E-638.553127
TATCAAC64500.033.0896842
ATCAACG66750.031.3253173
TCAACGC68150.030.7314854
CAACGCA69000.030.2768785
ATCTACG650.00533025729.7824442
TAAACGT650.00553466729.5540875
AACGCAG71450.029.3973776
GTACATG50200.029.253521
TACATGG50050.028.3319992
TTAGGGC856.240191E-428.0631224
CATCGGG350.00832770927.18479728-29
TGGTATC12600.026.8869292
ATGGGAT14750.026.6988625
ACATGGG50150.026.4634533
CATGGGG9950.026.3708744
ACGCAGA80250.026.0623917
CGCAGAG81600.025.9169458
Produced by FastQC (version 0.11.5)