FastQCFastQC Report
Fri 27 May 2016
SRR939509_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939509_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences458250
Sequences flagged as poor quality0
Sequence length101
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124212.7105291871249317No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119032.597490452809602No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98242.143807965084561No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA74991.636442989634479No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36090.7875613747954174No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29030.6334969994544463No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26130.5702127659574469No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16510.36028368794326243No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15010.32755046372067653No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14830.32362247681396616No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA7290.1590834697217676No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6890.150354609929078No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6850.14948172394980905No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6460.14097108565193672No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6220.13573376977632298No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50100.048.5048141
GTATCAA74000.032.8354872
TAACGCA600.004003876431.5766894
GCGGTAT856.504496E-427.8648221
TATCAAC99550.024.3605183
ATCAACG103750.023.6026214
TCAACGC105000.023.231425
CAACGCA106700.022.9500666
GAGTAAT1050.002229391622.5572381
GTACACT2103.5399353E-822.5547754
TGGTATC24750.022.3907432
AACGCAG109550.022.3530087
GTGGTAT25050.022.1250021
AGTACAC1954.3269392E-721.8607833
GAGTACT86650.021.12704312-13
AGTACTT86450.021.09373912-13
GTACTTT90050.020.35565414-15
GGACAAT1404.939487E-420.2993016
AGTACAT34300.020.29932
ACGCAGA124350.019.5783088
Produced by FastQC (version 0.11.5)