##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939509_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 458250 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.270064375340972 31.0 30.0 31.0 27.0 34.0 2 30.45933224222586 31.0 30.0 31.0 27.0 34.0 3 30.27143917075832 31.0 30.0 31.0 27.0 34.0 4 34.281021276595744 35.0 35.0 37.0 30.0 37.0 5 33.91678123295144 35.0 33.0 37.0 30.0 37.0 6 34.062769230769234 35.0 33.0 37.0 30.0 37.0 7 33.89687943262411 35.0 33.0 37.0 29.0 37.0 8 33.96034260774686 35.0 33.0 37.0 30.0 37.0 9 35.040451718494275 37.0 34.0 39.0 29.0 39.0 10-11 34.93383415166394 37.0 33.5 39.0 28.5 39.0 12-13 34.82030769230769 37.0 33.0 39.0 27.0 39.0 14-15 35.195458810692855 37.0 33.0 40.0 27.0 41.0 16-17 35.26184069830878 37.0 33.0 39.5 27.0 41.0 18-19 35.46736279323513 37.5 33.5 40.0 27.0 41.0 20-21 35.5360632842335 38.0 34.0 40.0 27.0 41.0 22-23 35.33264702673213 37.5 33.0 40.0 27.0 41.0 24-25 35.040638297872334 37.0 33.0 40.0 27.0 41.0 26-27 34.43668303327878 37.0 32.5 39.5 25.0 41.0 28-29 33.993974904528095 36.0 32.0 39.0 24.0 40.0 30-31 33.43097872340425 35.5 32.0 38.5 24.0 40.0 32-33 32.740866339334424 35.0 31.0 38.0 22.5 40.0 34-35 32.26575886524823 35.0 30.5 38.0 20.5 40.0 36-37 31.840367703218767 35.0 30.0 38.0 18.0 40.0 38-39 31.562801963993454 35.0 30.0 38.0 17.0 40.0 40-41 31.101424986361156 35.0 30.0 38.0 15.0 40.0 42-43 30.491235133660666 35.0 28.5 38.0 10.5 40.0 44-45 29.85157010365521 34.0 26.5 38.0 6.5 40.0 46-47 29.010678668848882 33.5 24.5 38.0 2.0 40.0 48-49 28.33221385706492 33.0 24.0 38.0 2.0 40.0 50-51 27.927311511183852 33.0 23.0 37.0 2.0 40.0 52-53 27.52495362793235 33.0 23.0 37.0 2.0 39.5 54-55 27.19074740861975 33.0 22.0 37.0 2.0 39.0 56-57 26.713368248772504 33.0 20.5 36.5 2.0 39.0 58-59 26.184510638297873 32.0 19.5 36.0 2.0 39.0 60-61 25.806671031096563 31.5 18.5 36.0 2.0 39.0 62-63 25.348820512820513 31.0 16.0 35.0 2.0 38.5 64-65 24.807754500818334 31.0 12.0 35.0 2.0 38.0 66-67 22.867008183306055 28.5 6.5 34.5 2.0 37.5 68-69 19.233496999454445 21.5 2.0 32.0 2.0 35.0 70-71 18.595312602291322 20.5 2.0 31.0 2.0 35.0 72-73 20.17055973813421 25.0 2.0 32.0 2.0 35.0 74-75 20.741590834697217 26.0 2.0 33.0 2.0 35.0 76-77 21.330388434260776 27.0 2.0 32.5 2.0 35.0 78-79 22.077574468085103 29.0 2.0 33.5 2.0 35.0 80-81 21.91096672122204 29.0 2.0 34.0 2.0 35.0 82-83 21.42365521003819 29.0 2.0 33.5 2.0 35.0 84-85 20.965800327332243 28.5 2.0 33.0 2.0 35.0 86-87 20.420963447899616 27.0 2.0 33.0 2.0 35.0 88-89 19.749090016366612 26.0 2.0 33.0 2.0 35.0 90-91 19.081321331151116 25.5 2.0 33.0 2.0 35.0 92-93 18.250810692853243 24.5 2.0 32.5 2.0 34.0 94-95 16.758209492635025 18.5 2.0 32.0 2.0 34.0 96-97 15.830128750681943 8.5 2.0 31.0 2.0 34.0 98-99 15.268365521003819 2.0 2.0 31.0 2.0 34.0 100-101 14.320884888161483 2.0 2.0 30.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3461159157911702 1101 2 0.06704710456136809 1101 3 0.39587287549939276 1101 4 0.15546075754434696 1101 5 0.020903116718464787 1101 6 0.5324307301023055 1101 7 0.541426959423859 1101 8 0.46661162173699466 1101 9 0.5075154961695887 1101 10-11 0.8038690706968055 1101 12-13 0.6891395058367848 1101 14-15 0.9435719242763199 1101 16-17 0.933078878208633 1101 18-19 0.7805855859172084 1101 20-21 0.6601209002427382 1101 22-23 0.5598097222666141 1101 24-25 0.5984317974058158 1101 26-27 0.40457126701032564 1101 28-29 0.5027596330732962 1101 30-31 1.136693540799513 1101 32-33 1.0374826516503362 1101 34-35 1.519924065263698 1101 36-37 1.2001189156205676 1101 38-39 1.169842627448844 1101 40-41 1.2131914633510448 1101 42-43 1.5449037020003935 1101 44-45 1.2361810434950726 1101 46-47 1.5514825700559562 1101 48-49 1.3491639037072893 1101 50-51 1.517848164577913 1101 52-53 1.4227950730305068 1101 54-55 0.9007597868923618 1101 56-57 1.1771707463997068 1101 58-59 0.9301884027930676 1101 60-61 0.715146707043143 1101 62-63 0.48923885474329154 1101 64-65 0.24101539438193598 1101 66-67 -3.1543133376173778 1101 68-69 -6.925065395308202 1101 70-71 -8.72148709518008 1101 72-73 -8.775265254031346 1101 74-75 -8.253494752901437 1101 76-77 -4.693041132775257 1101 78-79 -2.57938780624135 1101 80-81 -1.9481421372168093 1101 82-83 -1.3091270415525322 1101 84-85 -0.929259287326996 1101 86-87 -1.2727026258063212 1101 88-89 -1.2668545809324954 1101 90-91 -1.259909907734027 1101 92-93 -1.9376965277441975 1101 94-95 -4.7385341226933075 1101 96-97 -2.328494088421328 1101 98-99 -1.8833951831531959 1101 100-101 -1.4997538622664148 1102 1 0.433079384228062 1102 2 0.39050193489163476 1102 3 0.48660391908162737 1102 4 0.4763643570990439 1102 5 0.29244168926619096 1102 6 0.5301062753956742 1102 7 0.44200111519840135 1102 8 0.40283085234764116 1102 9 0.5208798222323452 1102 10-11 0.6951144836354075 1102 12-13 0.6021141893810906 1102 14-15 0.7191666960571297 1102 16-17 1.0107651438252603 1102 18-19 0.601682460530661 1102 20-21 1.0203108767404885 1102 22-23 1.0287980948390825 1102 24-25 0.7422980201411491 1102 26-27 1.0786450929287668 1102 28-29 0.8487116802427224 1102 30-31 0.7377789735294868 1102 32-33 1.0525993382458125 1102 34-35 0.9269974170139967 1102 36-37 1.3871937104913066 1102 38-39 1.0297645958651174 1102 40-41 1.0054491221973763 1102 42-43 1.3664592827532864 1102 44-45 1.0523274996035958 1102 46-47 1.4587363118610917 1102 48-49 0.995710207257364 1102 50-51 1.1125091343548483 1102 52-53 1.2405134996169096 1102 54-55 1.0260690269863737 1102 56-57 0.8079226121482748 1102 58-59 1.0724800780497397 1102 60-61 0.431944628949708 1102 62-63 0.34842637793629905 1102 64-65 0.6827313676635924 1102 66-67 -2.690221266533392 1102 68-69 -6.153751021828633 1102 70-71 -8.189408589428215 1102 72-73 -7.835690768400701 1102 74-75 -7.436711777599413 1102 76-77 -4.04016829230191 1102 78-79 -1.2731911501575688 1102 80-81 -0.5946394777520005 1102 82-83 -0.2004961505287426 1102 84-85 -0.3164098460207647 1102 86-87 -0.6654684692071378 1102 88-89 -0.6461813652478412 1102 90-91 -1.095203479663276 1102 92-93 -1.4144081716645367 1102 94-95 -4.276452112220376 1102 96-97 -1.5412796201081296 1102 98-99 -0.9689413011592176 1102 100-101 -0.7587888270130438 1103 1 0.2582504069465088 1103 2 0.16471569645929662 1103 3 0.35896478349077654 1103 4 0.07942334634312687 1103 5 0.6130400227980388 1103 6 0.2994081505607369 1103 7 0.3045304389271877 1103 8 0.2948677445761376 1103 9 0.5793771008867452 1103 10-11 0.5911570101627035 1103 12-13 0.3902699922427715 1103 14-15 0.720126028641296 1103 16-17 0.7240697014965534 1103 18-19 0.6052155490896212 1103 20-21 0.7126266356877906 1103 22-23 0.5878857320068107 1103 24-25 0.8968774414862324 1103 26-27 0.6926956255460723 1103 28-29 1.0412902523001222 1103 30-31 0.8879035184614708 1103 32-33 0.7262165269999983 1103 34-35 1.1788914839109417 1103 36-37 1.5203001355597898 1103 38-39 1.2893423859564166 1103 40-41 1.809891570010194 1103 42-43 1.9509543449739688 1103 44-45 1.3924115480129942 1103 46-47 1.2206807145891716 1103 48-49 0.6585119244756399 1103 50-51 0.40712263077903543 1103 52-53 0.778671891770319 1103 54-55 0.622391973807499 1103 56-57 0.49309063128832165 1103 58-59 0.7719655857839562 1103 60-61 0.45722776799985354 1103 62-63 0.6443901899951463 1103 64-65 0.2923438542640078 1103 66-67 -3.038268681643462 1103 68-69 -6.71770264350204 1103 70-71 -8.815764730969256 1103 72-73 -7.657909998552485 1103 74-75 -6.518199377481341 1103 76-77 -3.721031613948906 1103 78-79 -1.4875741478323832 1103 80-81 -0.965484311856077 1103 82-83 -0.11670537565730044 1103 84-85 -0.20541467763912635 1103 86-87 -0.2916899675784741 1103 88-89 -0.31121246264145697 1103 90-91 -0.2961765354935828 1103 92-93 -0.003197574211363019 1103 94-95 0.4483281863767452 1103 96-97 0.7118741372130923 1103 98-99 0.4231693858834493 1103 100-101 0.5805197285246759 1104 1 0.3499047211072188 1104 2 0.165688536306817 1104 3 0.3774256996499048 1104 4 0.40715005002890337 1104 5 0.5506912289914467 1104 6 0.7067285144028403 1104 7 0.7067049919968795 1104 8 0.24852472636611367 1104 9 0.5999526636617958 1104 10-11 0.788971192631621 1104 12-13 0.7530803729559707 1104 14-15 0.7420034047386181 1104 16-17 0.8753040560557181 1104 18-19 0.9298688468886951 1104 20-21 0.7842686101430658 1104 22-23 0.560484929669947 1104 24-25 0.6217519323453828 1104 26-27 1.1184909861240442 1104 28-29 0.969261702010698 1104 30-31 0.9550627493035648 1104 32-33 0.9268276240305298 1104 34-35 1.1631852405832142 1104 36-37 1.284570800086204 1104 38-39 1.4239053376549151 1104 40-41 0.9770497768103468 1104 42-43 1.0376382155453037 1104 44-45 1.4565024931781103 1104 46-47 1.5939849651312734 1104 48-49 1.0334789631255425 1104 50-51 1.2969088364220198 1104 52-53 1.4707347986669852 1104 54-55 1.2169020849416512 1104 56-57 1.1132145915657325 1104 58-59 1.1541585204065576 1104 60-61 1.344485870091713 1104 62-63 0.3512850644092005 1104 64-65 0.3712578065794965 1104 66-67 -2.7555409411152105 1104 68-69 -6.391655368605658 1104 70-71 -7.6969605047481515 1104 72-73 -7.391217474826714 1104 74-75 -6.773887290458257 1104 76-77 -3.792969280515731 1104 78-79 -0.94076263043263 1104 80-81 -0.23799211377564333 1104 82-83 -0.3974976769099605 1104 84-85 -0.4385019848952325 1104 86-87 0.08029070875003086 1104 88-89 0.1494566463652447 1104 90-91 0.21114280430657573 1104 92-93 0.5251581472389866 1104 94-95 0.8846007761020189 1104 96-97 1.2736571478452046 1104 98-99 1.5471578244498954 1104 100-101 0.8281650720779457 1105 1 0.31069759334559066 1105 2 0.2967028317794629 1105 3 0.7611199338668087 1105 4 0.4139488456285534 1105 5 0.7082250195234394 1105 6 0.5906212626008056 1105 7 0.8615730233546941 1105 8 0.4607743953039787 1105 9 0.9372143544090719 1105 10-11 0.9448663180653227 1105 12-13 1.179066716180479 1105 14-15 1.0788434074019122 1105 16-17 1.331650708320872 1105 18-19 0.9684175493963849 1105 20-21 0.9286006044317787 1105 22-23 0.9608531052894662 1105 24-25 0.9781313825726201 1105 26-27 1.226758785183506 1105 28-29 1.2613349524366981 1105 30-31 1.4826697557373905 1105 32-33 1.214365878044589 1105 34-35 1.4089998013987746 1105 36-37 1.56419562099207 1105 38-39 1.4827715145715672 1105 40-41 1.436360575724315 1105 42-43 1.4976740220422613 1105 44-45 1.6881214548390169 1105 46-47 1.5071036567638494 1105 48-49 1.7556580301344908 1105 50-51 1.8218673457669752 1105 52-53 1.4259717454479777 1105 54-55 1.7524496506177236 1105 56-57 1.83720864354434 1105 58-59 1.5850966161026285 1105 60-61 1.6410534277285826 1105 62-63 1.6743363599287058 1105 64-65 1.9263152276732676 1105 66-67 2.0402584537254 1105 68-69 -4.074404133750058 1105 70-71 -4.781051520325951 1105 72-73 -1.7013222063642957 1105 74-75 0.8429745610730137 1105 76-77 1.2713418513098809 1105 78-79 1.1085822734298816 1105 80-81 1.427596905565288 1105 82-83 1.659123195910393 1105 84-85 1.3209890795096086 1105 86-87 0.9854922682561345 1105 88-89 1.0077361589825955 1105 90-91 0.7970303097626257 1105 92-93 1.015444525234269 1105 94-95 1.968579588125312 1105 96-97 1.5402022401110553 1105 98-99 1.3358945603868424 1105 100-101 1.3793508540380817 1106 1 0.1668143587171258 1106 2 0.13256183515395037 1106 3 0.1265998834527906 1106 4 0.17879726476962077 1106 5 0.29490054026501156 1106 6 0.4915631374059899 1106 7 0.3098458011399998 1106 8 0.30507639153648114 1106 9 0.3160485979955112 1106 10-11 0.3871585296415816 1106 12-13 0.46273365840763603 1106 14-15 0.3266513462545859 1106 16-17 0.6945693005229643 1106 18-19 0.4315475552238155 1106 20-21 0.4815068641723954 1106 22-23 0.49562522579523716 1106 24-25 0.5998446460671829 1106 26-27 0.5499546544865197 1106 28-29 0.8548516012596963 1106 30-31 0.6580753039898326 1106 32-33 0.9972535713322017 1106 34-35 0.8726461443496198 1106 36-37 0.5086128105460723 1106 38-39 0.6203313134575765 1106 40-41 0.8611162981219636 1106 42-43 0.6998843785994744 1106 44-45 0.5196749384205823 1106 46-47 0.8296639299753004 1106 48-49 0.3927353722857596 1106 50-51 0.4035082407513215 1106 52-53 0.6338374164829546 1106 54-55 0.2896950394968236 1106 56-57 0.060835471391307294 1106 58-59 0.6192832834920736 1106 60-61 0.24554325683590505 1106 62-63 0.3361667140465272 1106 64-65 0.28003326152655106 1106 66-67 0.5565833802049553 1106 68-69 -4.837520587422013 1106 70-71 -5.7826330568693365 1106 72-73 -3.089010073119507 1106 74-75 -0.8584782299934055 1106 76-77 -0.3783525475842602 1106 78-79 -0.05649517713862906 1106 80-81 -0.13330512590281884 1106 82-83 0.13554922146975912 1106 84-85 0.4043592295319698 1106 86-87 0.42423923596134827 1106 88-89 0.6562284816865258 1106 90-91 0.5575623673863923 1106 92-93 0.7824152119219825 1106 94-95 1.7985976124920846 1106 96-97 1.2498174944172487 1106 98-99 0.7744517979154697 1106 100-101 0.3049481652904369 1107 1 0.14136533531030793 1107 2 0.22233509797009887 1107 3 -0.05113483927852869 1107 4 0.04866164137327189 1107 5 0.015488416196660637 1107 6 0.44669745169619546 1107 7 0.3862731632038461 1107 8 0.19549897099922475 1107 9 0.3399406578303896 1107 10-11 0.3867186699777534 1107 12-13 0.36789328208325855 1107 14-15 0.2996313954769789 1107 16-17 0.33028695251018547 1107 18-19 0.393031907041717 1107 20-21 0.13070830942557166 1107 22-23 0.06275269782400272 1107 24-25 0.44641915166143065 1107 26-27 0.5684619243892968 1107 28-29 0.815691872855588 1107 30-31 1.1103191119177822 1107 32-33 0.5185483645807025 1107 34-35 0.5862049930344035 1107 36-37 0.41592984114424425 1107 38-39 1.0309001637132909 1107 40-41 1.1356410925923015 1107 42-43 0.7360488721499401 1107 44-45 1.266834178091731 1107 46-47 1.0052656824320572 1107 48-49 1.0897872652532428 1107 50-51 0.8762997786892157 1107 52-53 0.6850007754747693 1107 54-55 0.855941046723089 1107 56-57 0.8022206802373724 1107 58-59 0.8478287792942076 1107 60-61 0.8089162124484766 1107 62-63 1.0183471612371875 1107 64-65 0.9880240454193121 1107 66-67 1.66094706734604 1107 68-69 -4.0747061837634995 1107 70-71 -5.219895454104506 1107 72-73 -1.831440821404577 1107 74-75 0.33204035058644266 1107 76-77 0.9448605225314637 1107 78-79 1.0214332933017545 1107 80-81 1.1751132993297695 1107 82-83 0.9371934587845203 1107 84-85 0.4506108222777776 1107 86-87 1.0199471132893763 1107 88-89 0.9250333550523209 1107 90-91 1.234525590231165 1107 92-93 1.269062329270259 1107 94-95 1.6536743261120286 1107 96-97 1.9916582722008496 1107 98-99 1.7435865660291352 1107 100-101 1.3717195604484882 1108 1 -0.028228478782434507 1108 2 0.12505557700072245 1108 3 -0.027749092325510816 1108 4 -0.12380824240521804 1108 5 0.08334322968432417 1108 6 -0.20678329985381794 1108 7 -0.010384780956393058 1108 8 -0.2023769808148259 1108 9 -0.3828408001640611 1108 10-11 0.010731494419630394 1108 12-13 0.2843851057157494 1108 14-15 0.25023136247289557 1108 16-17 0.03853679338337912 1108 18-19 0.0013638652051213285 1108 20-21 0.09763350803940085 1108 22-23 0.12672560304729075 1108 24-25 0.5266524590629871 1108 26-27 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1.396032317318241 2313 58-59 0.4023159587813865 2313 60-61 1.237663746965243 2313 62-63 1.0248806918091766 2313 64-65 0.1794688185948523 2313 66-67 2.18486225472277 2313 68-69 4.8446725144613545 2313 70-71 4.144749516309695 2313 72-73 3.3099546421062733 2313 74-75 2.7911133288901873 2313 76-77 1.680437445608387 2313 78-79 1.8402202755502728 2313 80-81 1.3001132993297695 2313 82-83 1.4330021372855057 2313 84-85 1.1091413942698871 2313 86-87 1.5432459298574237 2313 88-89 1.5976171814823061 2313 90-91 1.4738007381601612 2313 92-93 1.3790228815385035 2313 94-95 1.616322255106109 2313 96-97 1.6050705009976927 2313 98-99 1.5469642780606936 2313 100-101 1.2432678839198896 2314 1 -0.07012540719780702 2314 2 -0.271016050467896 2314 3 0.012405700688809418 2314 4 -0.4217419555811901 2314 5 -1.8329771518718871 2314 6 -3.8613978166968437 2314 7 -1.5993050854016957 2314 8 -0.24126801167346912 2314 9 0.017593305706363083 2314 10-11 0.28601030748279044 2314 12-13 0.04387046178252518 2314 14-15 -3.008347226397426 2314 16-17 -3.380864454671002 2314 18-19 -0.61683002589713 2314 20-21 8.807341885628261E-4 2314 22-23 -0.27123809770525753 2314 24-25 -2.2857074204155126 2314 26-27 -2.078979275161082 2314 28-29 -0.5166870798707635 2314 30-31 -0.5229124208174767 2314 32-33 -0.7290603817873453 2314 34-35 -0.9769113777742753 2314 36-37 -0.5925005208841299 2314 38-39 -1.8727286034390502 2314 40-41 -5.083102942528907 2314 42-43 -6.097549566907553 2314 44-45 -3.5685351672031693 2314 46-47 -4.4900366441357775 2314 48-49 -2.7312345582499695 2314 50-51 -1.718311152150001 2314 52-53 -3.380883299094169 2314 54-55 -2.3871184854183305 2314 56-57 -4.55711422252185 2314 58-59 -5.302571000137828 2314 60-61 -4.164838320859406 2314 62-63 -2.8974552607687087 2314 64-65 -1.9885936297095306 2314 66-67 -1.2860848089662298 2314 68-69 0.49393499033673294 2314 70-71 0.44324000431631205 2314 72-73 -0.9506429592171344 2314 74-75 -0.16952907530060557 2314 76-77 -0.3297465907853301 2314 78-79 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-2.28145683231973 2315 36-37 -6.340301107394396 2315 38-39 -3.573152194185223 2315 40-41 -2.844173323760579 2315 42-43 -3.1131083808534648 2315 44-45 -3.749693524974429 2315 46-47 -3.966152642832423 2315 48-49 -6.065349905268544 2315 50-51 -6.311395218621165 2315 52-53 -5.263165801537962 2315 54-55 -6.646909948435596 2315 56-57 -8.964486330700407 2315 58-59 -8.943488237022809 2315 60-61 -7.936636952335455 2315 62-63 -9.104460800032316 2315 64-65 -8.875397148771114 2315 66-67 -7.428191358330844 2315 68-69 -5.220304175515334 2315 70-71 -3.1879427913826106 2315 72-73 -4.401962607310974 2315 74-75 -5.434061496284636 2315 76-77 -6.935733883062943 2315 78-79 -6.444045458715459 2315 80-81 -6.58706618784972 2315 82-83 -9.09747571819235 2315 84-85 -7.66201245188396 2315 86-87 -7.630326331214839 2315 88-89 -6.712697503832381 2315 90-91 -5.832522105662683 2315 92-93 -6.258855906340283 2315 94-95 -4.371877045593191 2315 96-97 -3.4703956994685097 2315 98-99 -3.5377967942003767 2315 100-101 -2.975904610252604 2316 1 0.05343275507003398 2316 2 -0.25928584518930364 2316 3 0.006996772699238818 2316 4 0.10403191212815699 2316 5 0.09983483900462176 2316 6 -2.0105017593491716 2316 7 -0.15896621280475642 2316 8 -0.08199789111302636 2316 9 0.592047525321874 2316 10-11 0.41461897480113663 2316 12-13 -0.547885810618908 2316 14-15 -1.0739386242468854 2316 16-17 -1.3810273741887542 2316 18-19 -0.03198154104865125 2316 20-21 -4.5667634495846485 2316 22-23 -3.9237633502305087 2316 24-25 -2.630217032667062 2316 26-27 -1.8873207544703021 2316 28-29 -1.709160198150336 2316 30-31 0.1693651940407861 2316 32-33 -2.3306895769649287 2316 34-35 -3.316305317168215 2316 36-37 -5.32514959224288 2316 38-39 -3.3004249214579495 2316 40-41 -2.510082414669668 2316 42-43 -3.5994720172170993 2316 44-45 -1.7989359492168582 2316 46-47 -1.1964556731354499 2316 48-49 -1.4077741476927876 2316 50-51 -0.6000315822575288 2316 52-53 -2.771499134871295 2316 54-55 -1.53781903934469 2316 56-57 -0.8546378458519222 2316 58-59 -1.0472761158106856 2316 60-61 -1.8593642250627305 2316 62-63 -1.222642618214131 2316 64-65 -0.745094118468085 2316 66-67 0.1634753083358227 2316 68-69 2.0403018850907273 2316 70-71 3.296905693465874 2316 72-73 2.528340422992052 2316 74-75 1.3280597158365772 2316 76-77 -0.3364914588205181 2316 78-79 0.533984844314844 2316 80-81 0.07581260002906731 2316 82-83 -0.19596056667719708 2316 84-85 0.38344209357058645 2316 86-87 -0.0674475433360513 2316 88-89 0.6486661325312539 2316 90-91 0.7803566822161052 2316 92-93 1.5381137906294136 2316 94-95 1.4675168938007452 2316 96-97 1.5561194520466426 2316 98-99 -0.014311945715528296 2316 100-101 -0.16075309510108937 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 24.0 4 47.0 5 167.0 6 448.0 7 935.0 8 1544.0 9 2604.0 10 4019.0 11 5284.0 12 6335.0 13 8032.0 14 9783.0 15 12443.0 16 14099.0 17 14662.0 18 12212.0 19 9703.0 20 9084.0 21 9051.0 22 9368.0 23 9963.0 24 11319.0 25 13802.0 26 16534.0 27 19448.0 28 22187.0 29 25215.0 30 27360.0 31 29818.0 32 31290.0 33 32674.0 34 32849.0 35 28381.0 36 17301.0 37 8374.0 38 1832.0 39 53.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.117722168261835 24.171295740524496 17.86360511180714 14.847376979406532 2 19.28641424028739 27.043189223755054 35.21921984840449 18.451176687553062 3 22.076377523186032 29.374795417348608 32.95079105291871 15.598036006546645 4 15.086524822695036 22.40392798690671 34.1228587015821 28.386688488816148 5 10.822476813966176 42.60032733224223 32.10387343153301 14.473322422258592 6 26.30616475722859 40.88270594653574 15.398145117294055 17.412984178941628 7 22.532242225859246 40.45979268957992 19.63316966721222 17.374795417348608 8 27.418876159301693 35.187779596290234 18.106710310965628 19.28663393344244 9 26.830332787779597 17.355591925804692 20.38887070376432 35.42520458265139 10-11 24.815493726132022 30.91533006001091 23.550245499181667 20.718930714675395 12-13 27.354282596835787 29.49056192034916 26.873322422258596 16.281833060556465 14-15 23.442444080741954 28.820076377523186 29.657501363884343 18.079978177850517 16-17 17.34564836687598 31.861712284300513 30.91942672900561 19.873212619817892 18-19 16.821587602251636 26.45967382041683 39.468013254932686 17.25072532239885 20-21 18.37492457711356 25.27007667220586 40.941015756700224 15.413982993980355 22-23 17.768685215493726 25.843426077468628 40.93431533006001 15.453573376977634 24-25 17.11783960720131 26.240043644298964 41.251282051282054 15.390834697217675 26-27 16.976160314763714 26.38208308374168 41.270729745978436 15.371026855516167 28-29 16.74774869155656 26.468970574754792 41.42080152255237 15.362479211136284 30-31 16.58270237358709 26.916584557335227 41.28293511671528 15.217777952362402 32-33 16.703676343014774 27.02135576538011 40.872977334801355 15.40199055680376 34-35 16.971930961710747 26.816554436344077 40.858821128763154 15.352693473182027 36-37 17.1411193808201 26.957431381798486 40.5213192515331 15.380129985848315 38-39 16.96665003226782 26.989985509509452 40.66903042566226 15.374334032560471 40-41 17.11522016672364 27.244997253308316 40.21790056648468 15.421882013483362 42-43 16.938057040024983 27.640650900108977 39.64661741164343 15.774674648222604 44-45 17.2565381226244 27.851184893117757 38.80266995406308 16.089607030194767 46-47 17.492621278017577 28.404831138370557 37.46501726888467 16.637530314727197 48-49 17.757193040694226 28.788323845985015 36.56736708885639 16.887116024464362 50-51 18.05326548662903 29.430655499623278 35.5854508129593 16.93062820078839 52-53 18.263203058642173 29.823180437616408 34.96913627421225 16.944480229529173 54-55 17.806599654166593 29.790469495048296 35.112024697395775 17.290906153389344 56-57 18.17310662244211 29.99127951077946 34.5021408309895 17.33347303578893 58-59 18.452894098749212 29.544229371593943 34.67549559026694 17.327380939389904 60-61 18.39088988599263 29.646716832236326 34.88878607468966 17.073607207081377 62-63 18.855327232514522 29.382894939273683 35.036427060226586 16.725350767985216 64-65 18.757829037819434 29.488030028588263 34.91368963184427 16.84045130174803 66-67 19.861915592177265 29.043015398516953 34.70807298120639 16.38699602809939 68-69 21.631751696184686 27.796061326989165 34.746233148295005 15.825953828531148 70-71 21.716017575746882 27.878557941731813 34.67868234837389 15.726742134147417 72-73 19.7591097371947 29.309446453748546 34.07777320454326 16.85367060451349 74-75 18.74252596645949 30.228981432697076 33.8910665760127 17.137426024830734 76-77 18.154527091838236 30.52446411598644 34.116957647621135 17.20405114455419 78-79 17.98826098200519 30.33245274330528 34.15750885773179 17.521777416957743 80-81 17.883169866000067 30.6491157051936 33.75159439297409 17.71612003583224 82-83 18.08630660120022 30.69132569558101 33.392689579923626 17.829678123295146 84-85 17.96388434260775 30.422804146208403 33.264593562465905 18.348717948717947 86-87 17.98574574085268 30.42090653771203 33.147917398619526 18.445430322815763 88-89 18.143430911394145 30.225958186442508 33.18025045362942 18.450360448533928 90-91 17.873817881455892 30.41684988799403 33.34340823312081 18.365923997429263 92-93 17.78739664326569 30.54034649738239 33.23716892565734 18.435087933694568 94-95 18.058129434699563 29.910140925808832 33.43131699226998 18.60041264722163 96-97 18.174308815002025 29.81400641130841 33.35751249986324 18.654172273826326 98-99 17.798523546326052 30.123037806162255 32.99115546413353 19.087283183378165 100-101 17.8351399658242 30.157741167433038 33.069449324449664 18.937669542293094 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5956.0 1 4143.0 2 2396.0 3 2521.5 4 2342.5 5 2189.5 6 3241.0 7 6344.0 8 8930.0 9 9437.0 10 8201.0 11 5982.0 12 4629.0 13 3882.5 14 3471.5 15 3165.0 16 2843.5 17 2697.0 18 2685.0 19 2741.5 20 2791.0 21 2847.5 22 3065.5 23 3369.0 24 3553.5 25 3859.5 26 4326.0 27 4854.5 28 5483.0 29 6125.0 30 6773.5 31 7684.0 32 8675.5 33 9498.5 34 10260.0 35 10924.0 36 11620.0 37 12256.5 38 12401.0 39 12403.0 40 12405.5 41 12574.5 42 12642.5 43 12485.0 44 12591.0 45 12764.5 46 12948.0 47 12984.0 48 13272.5 49 13102.5 50 12053.0 51 11003.5 52 10030.0 53 9139.0 54 8493.5 55 7858.0 56 7065.5 57 6279.0 58 5621.0 59 5088.5 60 4492.0 61 3831.0 62 3119.5 63 2312.5 64 1633.0 65 1191.5 66 907.5 67 692.0 68 505.0 69 365.5 70 269.5 71 213.0 72 185.5 73 159.5 74 112.0 75 84.5 76 63.0 77 40.0 78 29.0 79 17.0 80 11.5 81 11.0 82 6.5 83 3.5 84 1.0 85 0.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02073104200763775 2 0.012438625204582653 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 5.455537370430988E-4 18-19 0.0012002182214948171 20-21 1.0911074740861975E-4 22-23 0.0 24-25 0.0 26-27 3.273322422258593E-4 28-29 0.015711947626841243 30-31 0.00425531914893617 32-33 0.007965084560829241 34-35 0.0088379705400982 36-37 0.07725040916530279 38-39 0.07932351336606655 40-41 0.09307146753955264 42-43 0.07877795962902345 44-45 0.07408619749045281 46-47 0.07539552645935625 48-49 0.07845062738679759 50-51 0.07746863066012001 52-53 0.058701582105837424 54-55 0.049318057828696124 56-57 0.029132569558101472 58-59 0.012875068194217132 60-61 0.007419530823786142 62-63 0.0012002182214948171 64-65 0.0043644298963447905 66-67 0.5012547735951991 68-69 0.9361702127659575 70-71 1.0688488816148392 72-73 0.3242771412984179 74-75 0.1817785051827605 76-77 0.01276595744680851 78-79 0.0075286415711947625 80-81 7.637752318603383E-4 82-83 0.0 84-85 0.0 86-87 2.182214948172395E-4 88-89 3.273322422258593E-4 90-91 0.0033824331696672124 92-93 0.0015275504637206766 94-95 0.2620840152755046 96-97 0.27168576104746317 98-99 0.012547735951991271 100-101 0.006764866339334425 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 458250.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.78621160869611 #Duplication Level Percentage of deduplicated Percentage of total 1 84.3722919516542 51.286719924839396 2 9.519545679977123 11.573142362434762 3 2.7945005608444604 5.0960130729633395 4 1.139854777312275 2.771498147875485 5 0.602358193536409 1.830753630826804 6 0.34719429802045504 1.2662775641282449 7 0.25813741301483634 1.098383678814886 8 0.1631301227947083 0.7932849731161375 9 0.1153073938812241 0.6308189680060223 >10 0.6203398256213589 6.763413878521489 >50 0.03817121410093238 1.5565599952203126 >100 0.0237669823453684 2.4669429243185044 >500 0.001800528965558212 0.7378939306013912 >1k 0.0021606347586698546 3.011990651164385 >5k 7.202115862232848E-4 3.791912358293651 >10k+ 7.202115862232848E-4 5.324393938875182 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12421 2.7105291871249317 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11903 2.597490452809602 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9824 2.143807965084561 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7499 1.636442989634479 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3609 0.7875613747954174 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2903 0.6334969994544463 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2613 0.5702127659574469 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1651 0.36028368794326243 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1501 0.32755046372067653 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1483 0.32362247681396616 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 729 0.1590834697217676 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 689 0.150354609929078 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 685 0.14948172394980905 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 646 0.14097108565193672 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 622 0.13573376977632298 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.182214948172395E-4 0.0 12-13 0.0 0.0 0.0 2.182214948172395E-4 0.0 14-15 0.0 0.0 0.0 2.182214948172395E-4 0.0 16-17 0.0 0.0 0.0 2.182214948172395E-4 0.0 18-19 0.0 0.0 0.0 2.182214948172395E-4 0.0 20-21 0.0 0.0 0.0 2.182214948172395E-4 0.0 22-23 0.0 0.0 0.0 4.36442989634479E-4 0.0 24-25 0.0 0.0 0.0 5.455537370430988E-4 0.0 26-27 0.0 0.0 0.0 6.546644844517185E-4 0.0 28-29 0.0 0.0 0.0 8.728859792689579E-4 0.0 30-31 0.0 0.0 0.0 0.0019639934533551553 0.0 32-33 0.0 0.0 0.0 0.00458265139116203 0.0 34-35 0.0 0.0 0.0 0.00861974904528096 0.0 36-37 0.0 0.0 0.0 0.0171303873431533 0.0 38-39 0.0 0.0 0.0 0.02629569012547736 0.0 40-41 0.0 0.0 0.0 0.037315875613747954 0.0 42-43 0.0 0.0 0.0 0.04800872885979269 0.0 44-45 0.0 0.0 0.0 0.06339334424440807 0.0 46-47 0.0 0.0 0.0 0.08074195308237861 0.0 48-49 0.0 0.0 0.0 0.10376432078559739 0.0 50-51 0.0 0.0 0.0 0.12940534642662302 0.0 52-53 0.0 0.0 0.0 0.15897435897435896 0.0 54-55 0.0 0.0 0.0 0.19072558647026733 0.0 56-57 0.0 0.0 0.0 0.225531914893617 0.0 58-59 0.0 0.0 0.0 0.2521549372613202 0.0 60-61 0.0 0.0 0.0 0.28412438625204584 0.0 62-63 0.0 0.0 0.0 0.31620294599018006 0.0 64-65 0.0 0.0 0.0 0.3464266230223677 0.0 66-67 0.0 0.0 0.0 0.37817785051827607 0.0 68-69 0.0 0.0 0.0 0.4262956901254774 0.0 70-71 0.0 0.0 0.0 0.49023458810692855 0.0 72-73 0.0 0.0 0.0 0.576759410801964 0.0 74-75 0.0 1.0911074740861975E-4 0.0 0.6644844517184942 0.0 76-77 0.0 2.182214948172395E-4 0.0 0.765302782324059 0.0 78-79 0.0 2.182214948172395E-4 0.0 0.8734315330060012 0.0 80-81 0.0 2.182214948172395E-4 0.0 1.0096017457719584 0.0 82-83 0.0 2.182214948172395E-4 0.0 1.1444626295690126 0.0 84-85 0.0 2.182214948172395E-4 0.0 1.278887070376432 0.0 86-87 0.0 2.182214948172395E-4 0.0 1.4192034915439171 0.0 88-89 0.0 2.182214948172395E-4 0.0 1.5719585379159846 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5010 0.0 48.504814 1 GTATCAA 7400 0.0 32.835487 2 TAACGCA 60 0.0040038764 31.576689 4 GCGGTAT 85 6.504496E-4 27.864822 1 TATCAAC 9955 0.0 24.360518 3 ATCAACG 10375 0.0 23.602621 4 TCAACGC 10500 0.0 23.23142 5 CAACGCA 10670 0.0 22.950066 6 GAGTAAT 105 0.0022293916 22.557238 1 GTACACT 210 3.5399353E-8 22.554775 4 TGGTATC 2475 0.0 22.390743 2 AACGCAG 10955 0.0 22.353008 7 GTGGTAT 2505 0.0 22.125002 1 AGTACAC 195 4.3269392E-7 21.860783 3 GAGTACT 8665 0.0 21.127043 12-13 AGTACTT 8645 0.0 21.093739 12-13 GTACTTT 9005 0.0 20.355654 14-15 GGACAAT 140 4.939487E-4 20.299301 6 AGTACAT 3430 0.0 20.2993 2 ACGCAGA 12435 0.0 19.578308 8 >>END_MODULE