FastQCFastQC Report
Fri 27 May 2016
SRR939508_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939508_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences436761
Sequences flagged as poor quality0
Sequence length101
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78331.7934293583905156No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56461.2926978370321525No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA54641.251027449795197No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47311.083201109989216No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14940.34206350841764716No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14660.33565267961196166No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14090.32260206382895906No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13220.3026827028970077No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9160.20972568521456814No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7730.17698466667124582No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG200.001902151972.813342
GTACGAG250.00481970857.5868873
TACGAGT250.00496100957.1680954
GGTATCA24550.054.5478321
GTATCAA47850.047.4732321
GTGGTAT10450.036.545991
GCGGTAT550.002085201236.0713651
TATCAAC63200.036.0226332
TGGTATC10850.034.8967172
ATCAACG65150.034.6935583
TCAACGC66400.033.8646354
CAACGCA67050.032.998725
CGGTATC600.003550129732.361482
AACGCAG68650.032.355226
ACGCAGA76950.028.488817
GTAACTC856.028443E-428.2288653
CGCAGAG77750.028.1956778
CAACGCG1204.709911E-628.18045
GTACATG45100.028.1532611
CGGGTCG1056.1661915E-527.5923736