FastQCFastQC Report
Fri 27 May 2016
SRR939508_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939508_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences436761
Sequences flagged as poor quality0
Sequence length101
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112902.584937757721042No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108502.484196162203127No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95452.185405748223857No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA74291.7009302570513394No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34840.7976902699645803No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26130.5982677024734351No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26060.5966649952720138No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14980.34297934110417366No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14540.3329051815523822No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13230.30291166106863937No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6680.15294405864992525No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6110.13989344286692262No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6060.13874865200876452No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6040.13829073566550126No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA5490.1256980362257619No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCACGC353.4542609E-667.721493
TATCACG407.622435E-659.263092
GGTATCA43450.047.9014931
TCACGCA502.8557539E-547.4104734
CCCGCTG958.8521665E-734.9140329
CGTATCA550.002601839634.4803432
GTATCAA67300.033.6029661
TCCCGCT1102.7811384E-630.1530288
CACGCAG804.5542463E-429.614585
GTGTAAC1153.9144234E-628.858551
GGTACTC1005.0140974E-528.4430243
GTGGTAC1205.4510347E-627.6561071
TACTCCC1205.475369E-627.6402745
TGGTATC21300.027.377882
CCGTCAG350.00838320827.14767892-93
GATATAG1405.888487E-727.0916981
GTGGTAT21650.026.9352781
CTCCCGC1257.5194403E-626.5346647
TATCAAC93750.026.094721
ATCAACG96900.025.0507352
Produced by FastQC (version 0.11.5)