##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939508_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 436761 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.130629795242708 31.0 30.0 31.0 27.0 33.0 2 30.326281421647078 31.0 30.0 31.0 27.0 34.0 3 30.14627221752858 31.0 30.0 31.0 26.0 34.0 4 34.14637982786925 35.0 33.0 37.0 30.0 37.0 5 33.71160657659452 35.0 33.0 37.0 28.0 37.0 6 33.942547525992474 35.0 33.0 37.0 30.0 37.0 7 33.76483019317201 35.0 33.0 37.0 29.0 37.0 8 33.70751509406747 35.0 33.0 37.0 29.0 37.0 9 34.903379651571456 37.0 33.0 39.0 28.0 39.0 10-11 34.712164089742444 37.0 33.0 39.0 27.5 39.0 12-13 34.64246922229778 37.0 33.0 39.0 27.0 39.0 14-15 34.95137271871802 37.0 33.0 39.5 27.0 40.5 16-17 34.90234476063568 37.0 32.5 39.0 27.0 40.0 18-19 35.19227907253624 37.0 33.0 39.5 27.0 40.0 20-21 35.218084948060834 37.0 33.0 40.0 27.0 41.0 22-23 35.01766526773224 37.0 33.0 40.0 27.0 41.0 24-25 34.77007333530237 37.0 33.0 40.0 26.5 41.0 26-27 34.08457142464643 36.5 32.0 39.0 24.5 40.0 28-29 33.675096906546145 36.0 32.0 39.0 24.0 40.0 30-31 33.02782299701667 35.5 31.0 38.5 23.5 40.0 32-33 32.217907505477825 35.0 30.0 38.0 20.5 40.0 34-35 31.922733485819474 35.0 30.0 38.0 19.5 40.0 36-37 31.440816602214944 35.0 30.0 38.0 17.0 40.0 38-39 31.010588170647104 35.0 30.0 38.0 15.0 40.0 40-41 30.577075334107214 34.5 28.5 38.0 12.5 40.0 42-43 30.044238153131804 34.0 27.0 38.0 9.0 40.0 44-45 29.2551395385577 33.5 25.5 38.0 4.5 40.0 46-47 28.32717206893473 33.0 23.5 37.0 2.0 40.0 48-49 27.569202607375658 33.0 22.5 37.0 2.0 39.5 50-51 27.148489677420834 33.0 22.0 37.0 2.0 39.0 52-53 26.915468643033606 33.0 21.5 37.0 2.0 39.0 54-55 26.474475743026506 33.0 20.0 36.5 2.0 39.0 56-57 25.91537820455581 31.5 18.5 36.0 2.0 39.0 58-59 25.369277476697782 31.0 16.0 35.0 2.0 39.0 60-61 24.934003951818042 31.0 13.5 35.0 2.0 38.0 62-63 24.610040731658735 31.0 11.0 35.0 2.0 38.0 64-65 24.03369233974645 30.5 8.0 35.0 2.0 38.0 66-67 22.95596447485009 29.0 4.5 34.5 2.0 37.5 68-69 19.736900730605527 22.5 2.0 33.0 2.0 36.0 70-71 18.504047980474446 21.0 2.0 31.5 2.0 35.0 72-73 19.255488699769437 22.5 2.0 32.0 2.0 35.0 74-75 19.802796037191968 24.5 2.0 32.0 2.0 35.0 76-77 19.843331936688486 25.0 2.0 31.5 2.0 34.5 78-79 20.848460599733038 27.0 2.0 33.0 2.0 35.0 80-81 20.80371644904192 27.5 2.0 33.0 2.0 35.0 82-83 20.374212670087303 27.0 2.0 33.0 2.0 35.0 84-85 19.902413448087167 26.0 2.0 33.0 2.0 35.0 86-87 19.331485640888268 26.0 2.0 33.0 2.0 35.0 88-89 18.65928047604983 25.0 2.0 32.5 2.0 34.5 90-91 17.88431201503797 23.5 2.0 32.0 2.0 34.0 92-93 17.15062356758044 20.0 2.0 32.0 2.0 34.0 94-95 16.175697921746675 15.5 2.0 31.0 2.0 34.0 96-97 15.367554566456255 4.5 2.0 31.0 2.0 34.0 98-99 14.456935257497808 2.0 2.0 31.0 2.0 34.0 100-101 13.46999846598025 2.0 2.0 30.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3921851513799197 1101 2 0.22400581312463785 1101 3 0.1466430497093576 1101 4 0.28971048870019445 1101 5 0.5960062717705981 1101 6 0.4041818380703859 1101 7 0.5806301401006806 1101 8 0.6615369864605753 1101 9 0.7489939335400848 1101 10-11 0.4526330334091213 1101 12-13 0.6614311502768402 1101 14-15 0.7649041266431453 1101 16-17 0.6731090023908521 1101 18-19 0.3755802366196619 1101 20-21 0.4523307069767597 1101 22-23 0.34983763607258567 1101 24-25 0.3914743812120989 1101 26-27 0.26200641742259023 1101 28-29 0.3553867622725235 1101 30-31 0.3789391835649667 1101 32-33 0.6738563549882457 1101 34-35 0.6740144625204927 1101 36-37 0.7865533213085563 1101 38-39 0.6768386575402268 1101 40-41 0.868462600932201 1101 42-43 0.7774772380478545 1101 44-45 0.9764444337470906 1101 46-47 1.2001986527372246 1101 48-49 0.8957209044933379 1101 50-51 0.7209767937147227 1101 52-53 0.28133753002927975 1101 54-55 0.36406162235107686 1101 56-57 0.48117930212075066 1101 58-59 0.9537748394205785 1101 60-61 0.492092948208267 1101 62-63 0.5881001637260397 1101 64-65 0.17982195370163723 1101 66-67 -0.5415816235306572 1101 68-69 -6.078951170296447 1101 70-71 -5.735996101003845 1101 72-73 -4.884795690344905 1101 74-75 -3.0496927297092213 1101 76-77 -0.5737491834985775 1101 78-79 -0.16703977130869063 1101 80-81 -0.15404966108671303 1101 82-83 -0.503550445494998 1101 84-85 -1.0209477580265052 1101 86-87 -0.966106397042207 1101 88-89 -0.9498693364165618 1101 90-91 -1.0140654609364823 1101 92-93 -0.8930328085261294 1101 94-95 -0.846839224199023 1101 96-97 -0.919503618488978 1101 98-99 -0.843229838861479 1101 100-101 -0.761844563038597 1102 1 0.10835144542868136 1102 2 0.3735959334269907 1102 3 0.3686967066879596 1102 4 0.29482600025134786 1102 5 0.5246499425892921 1102 6 0.48617374693868953 1102 7 0.46273383434107274 1102 8 0.4732691274166072 1102 9 0.493101247497286 1102 10-11 0.4746307977016855 1102 12-13 0.5061293297722074 1102 14-15 0.911068823758022 1102 16-17 0.3115046161457755 1102 18-19 0.1406313385040434 1102 20-21 0.21855289371156772 1102 22-23 0.5970110630927081 1102 24-25 0.6648188930826322 1102 26-27 0.6684500456563924 1102 28-29 0.38248939189031717 1102 30-31 0.4995369691499789 1102 32-33 0.8923408647295474 1102 34-35 0.5225612526833849 1102 36-37 0.9841925207123658 1102 38-39 1.300837912304413 1102 40-41 0.9911843085868348 1102 42-43 0.3789969867969205 1102 44-45 0.45397823551436645 1102 46-47 0.8204344666411956 1102 48-49 1.0111712398494568 1102 50-51 0.6362913896904452 1102 52-53 0.48021701475194334 1102 54-55 0.31962427539057714 1102 56-57 0.18964571327799362 1102 58-59 0.29967604244410495 1102 60-61 0.29202374773982953 1102 62-63 0.49090012114113435 1102 64-65 0.5659951677972437 1102 66-67 -0.2618387298864526 1102 68-69 -5.897579404087569 1102 70-71 -6.252825122615686 1102 72-73 -2.234979337741901 1102 74-75 -0.4476859161246374 1102 76-77 0.43032299791311246 1102 78-79 0.08837383456802783 1102 80-81 0.19784802868226237 1102 82-83 -0.1888985770823517 1102 84-85 -0.41537179720461737 1102 86-87 -0.6435949523493498 1102 88-89 -0.5623919596466287 1102 90-91 -0.293837631701944 1102 92-93 -0.7573120590318254 1102 94-95 -0.6610732282445895 1102 96-97 -1.0241427114305282 1102 98-99 -0.6303106437161574 1102 100-101 -0.8480789929331998 1103 1 0.3863972998674967 1103 2 0.14882920455469062 1103 3 0.32885399807692295 1103 4 0.3035036862017577 1103 5 0.389659273434404 1103 6 0.2789356770906508 1103 7 0.23223530061673614 1103 8 0.38251999385222035 1103 9 0.473746408787612 1103 10-11 0.5352612962354115 1103 12-13 0.1658334086844988 1103 14-15 0.031123475850790783 1103 16-17 0.5076656398727764 1103 18-19 0.443250640023642 1103 20-21 0.397778433567602 1103 22-23 0.42283084448433783 1103 24-25 0.3153854086784804 1103 26-27 0.42906721441138274 1103 28-29 0.45747606211159564 1103 30-31 0.5287891685634705 1103 32-33 1.2888551275927824 1103 34-35 1.154699349190981 1103 36-37 1.2293471461455887 1103 38-39 1.5269709434960923 1103 40-41 1.3049339753423688 1103 42-43 0.9733518255066009 1103 44-45 1.5291515226378003 1103 46-47 1.0758130323570043 1103 48-49 1.1041531113503886 1103 50-51 0.9863846981415278 1103 52-53 0.8679602832136872 1103 54-55 0.7162518413729835 1103 56-57 0.9325076167703941 1103 58-59 0.9222566876053939 1103 60-61 0.912818202551879 1103 62-63 0.10365671938160759 1103 64-65 0.2640690147713798 1103 66-67 -0.07982726627674808 1103 68-69 -5.51040931611103 1103 70-71 -6.605897636611953 1103 72-73 -1.988678351338276 1103 74-75 -0.29617965112863587 1103 76-77 -0.3731027552193851 1103 78-79 -0.35781384871642885 1103 80-81 -0.449879244045011 1103 82-83 -0.6791211843870677 1103 84-85 -1.1196439912861962 1103 86-87 -0.9350505641861915 1103 88-89 -0.9917507972899209 1103 90-91 -1.2887456562287376 1103 92-93 -0.8290595930227624 1103 94-95 -0.8957973018249685 1103 96-97 -1.008007014283102 1103 98-99 -0.6331365568060008 1103 100-101 -0.5432536130345049 1104 1 0.23925845974702753 1104 2 0.0840923399120328 1104 3 0.11281894190997477 1104 4 0.15898834912871962 1104 5 0.3064065864134662 1104 6 0.31109714099964236 1104 7 0.34569110215543475 1104 8 0.16457226408159897 1104 9 0.19084485438346377 1104 10-11 -0.09877858443659449 1104 12-13 -0.06842914145181567 1104 14-15 0.20651564530796662 1104 16-17 0.4744607696919374 1104 18-19 0.3114974034658857 1104 20-21 0.17925404697915326 1104 22-23 0.09481874040064042 1104 24-25 0.20250483658923457 1104 26-27 0.647046346285947 1104 28-29 0.2113949019465906 1104 30-31 0.2879193302843035 1104 32-33 0.7697198273458454 1104 34-35 0.896791014787123 1104 36-37 1.108724528066535 1104 38-39 0.9573410601120891 1104 40-41 1.1875643297579295 1104 42-43 0.48405994295046995 1104 44-45 0.7872739622958029 1104 46-47 0.842100019039659 1104 48-49 0.8460492274810782 1104 50-51 0.2787282942533764 1104 52-53 0.7053145132198217 1104 54-55 0.6542640881935178 1104 56-57 0.7893796817442009 1104 58-59 0.8167299173117861 1104 60-61 0.9723123432047593 1104 62-63 0.38191076240248734 1104 64-65 0.01604948837220377 1104 66-67 0.3206937231750011 1104 68-69 -5.523456890298501 1104 70-71 -4.133822671225637 1104 72-73 -0.5919170501492772 1104 74-75 -0.15135742985799183 1104 76-77 0.37281283021575007 1104 78-79 0.08115616227430777 1104 80-81 -0.07438858617093302 1104 82-83 -0.15199262131880076 1104 84-85 -0.38053838030116793 1104 86-87 -0.5571305253617744 1104 88-89 -0.47773403998536423 1104 90-91 -0.4733072400366716 1104 92-93 -0.4534775835735836 1104 94-95 -0.33601055065809504 1104 96-97 -0.26441375717123883 1104 98-99 0.1885037749144054 1104 100-101 0.3222369021027358 1105 1 0.22120562051091142 1105 2 0.36417242370340475 1105 3 0.11832161291451371 1105 4 0.1684395959579632 1105 5 0.6595538608765565 1105 6 0.49832389986153913 1105 7 0.47042037135994264 1105 8 0.3326493570894087 1105 9 0.3882365697893988 1105 10-11 0.4142334868355064 1105 12-13 0.4846479979007725 1105 14-15 0.5429849758729688 1105 16-17 0.4899569215298172 1105 18-19 0.3983135475178372 1105 20-21 0.529801378909788 1105 22-23 0.7291969941908718 1105 24-25 0.416358220222115 1105 26-27 0.6553405182745422 1105 28-29 0.2729345993460299 1105 30-31 0.8637054151076242 1105 32-33 0.8151624355068492 1105 34-35 0.6718226064833708 1105 36-37 0.7782213163728215 1105 38-39 0.9621902696245108 1105 40-41 1.1172345711524763 1105 42-43 1.2661961904439885 1105 44-45 1.4580653149765403 1105 46-47 1.6950016661333756 1105 48-49 1.801391391041097 1105 50-51 2.0910550806131774 1105 52-53 1.5194734634795104 1105 54-55 1.2862957509517763 1105 56-57 1.830947865739187 1105 58-59 1.482006240378201 1105 60-61 1.4121691687104594 1105 62-63 1.4214644930033309 1105 64-65 1.396396518134825 1105 66-67 1.3215402879449272 1105 68-69 -2.6487407521168826 1105 70-71 -2.5770518523750496 1105 72-73 1.081387734795463 1105 74-75 1.2564689687504291 1105 76-77 1.5798844267318586 1105 78-79 1.7924219619844983 1105 80-81 1.420343556410348 1105 82-83 1.4591437219539465 1105 84-85 1.6070891064828459 1105 86-87 1.4436835980574863 1105 88-89 1.4152481234510716 1105 90-91 1.3749661076944442 1105 92-93 1.5713292359072923 1105 94-95 1.226286111590369 1105 96-97 0.9855159532356375 1105 98-99 0.9635724424399967 1105 100-101 0.8054786272410759 1106 1 0.1277975805473659 1106 2 0.18132569605632654 1106 3 0.4127928722387786 1106 4 0.41086373610040283 1106 5 0.3868836613963538 1106 6 0.4573545138285269 1106 7 0.10856463166304309 1106 8 0.5215344956454544 1106 9 0.8284633899196834 1106 10-11 0.9351755329420897 1106 12-13 0.6084421721337421 1106 14-15 0.19370728997834874 1106 16-17 0.4908716277099856 1106 18-19 0.48970315834579736 1106 20-21 0.8234372515934822 1106 22-23 0.8681918299825426 1106 24-25 0.9356801225409441 1106 26-27 1.141167635431188 1106 28-29 1.066832057745465 1106 30-31 1.0234718443782143 1106 32-33 1.4146932688683194 1106 34-35 1.38512972499014 1106 36-37 1.766100615660637 1106 38-39 1.4392584844285743 1106 40-41 1.2061112714596298 1106 42-43 1.3827857877707501 1106 44-45 1.4650707492088273 1106 46-47 1.5780272846570185 1106 48-49 1.537631982393581 1106 50-51 1.6331568796478457 1106 52-53 1.3538171364804903 1106 54-55 1.0399225103266687 1106 56-57 1.4020772409712379 1106 58-59 1.7873510272280377 1106 60-61 1.6023706740940646 1106 62-63 0.9914783317315177 1106 64-65 1.4150669876389905 1106 66-67 1.6281643133271793 1106 68-69 -2.4892257826633433 1106 70-71 -2.6408500769674816 1106 72-73 0.6283651540414219 1106 74-75 1.772064540357409 1106 76-77 1.675727745326384 1106 78-79 1.628635550940519 1106 80-81 1.5162442550973587 1106 82-83 1.8582560181702341 1106 84-85 1.328009213142309 1106 86-87 1.6045486873341552 1106 88-89 1.6950182655511803 1106 90-91 1.8938677852183226 1106 92-93 1.7371340760868605 1106 94-95 1.5926090602520624 1106 96-97 1.4061829124282639 1106 98-99 1.1975043288949188 1106 100-101 0.7170457788257778 1107 1 -0.05244567533911493 1107 2 0.17692837810841056 1107 3 0.22513498981246016 1107 4 -0.2121987931370839 1107 5 0.47313034781456764 1107 6 0.35786129692536406 1107 7 0.026863399790286735 1107 8 0.2560737128604842 1107 9 0.4369695492834751 1107 10-11 0.04827782516102985 1107 12-13 0.4112879541390413 1107 14-15 0.3509581865945961 1107 16-17 0.5628309291289781 1107 18-19 0.2211432020071058 1107 20-21 0.1393073591874341 1107 22-23 0.4038225800215258 1107 24-25 0.33852590454625187 1107 26-27 0.7036539912708903 1107 28-29 0.43640168194630036 1107 30-31 0.3446982594725583 1107 32-33 0.44980554081592317 1107 34-35 0.5766001756372638 1107 36-37 0.8291405345753375 1107 38-39 0.7910205302729558 1107 40-41 0.307413314185343 1107 42-43 0.37355843707684855 1107 44-45 0.6351432581749883 1107 46-47 1.2665155116958502 1107 48-49 1.156839061763609 1107 50-51 1.1667565989679751 1107 52-53 0.7092825972632717 1107 54-55 0.8148055603812452 1107 56-57 1.2126729060265973 1107 58-59 1.0635790016549826 1107 60-61 1.1475289463535354 1107 62-63 0.7962187028526806 1107 64-65 0.8787384362589812 1107 66-67 0.6641809981860618 1107 68-69 -2.8535911342928486 1107 70-71 -2.6191207771078204 1107 72-73 0.5985117313063526 1107 74-75 1.64514266292095 1107 76-77 1.772765013375654 1107 78-79 1.4114010273166286 1107 80-81 1.1875174501698673 1107 82-83 1.7714986503236752 1107 84-85 1.2760171657385975 1107 86-87 1.2068915845741408 1107 88-89 1.5402739960985095 1107 90-91 1.2862543437712617 1107 92-93 1.0756924730929391 1107 94-95 1.7221779047866015 1107 96-97 1.3771169526590477 1107 98-99 0.8581857572435858 1107 100-101 1.26025878365971 1108 1 -0.09309278817874045 1108 2 0.05879517441370652 1108 3 0.09638312175577823 1108 4 0.06029440983740386 1108 5 -0.3222296694705804 1108 6 -0.12254436396158042 1108 7 0.026174694556125644 1108 8 0.08337524871816981 1108 9 -0.1184104539574875 1108 10-11 -0.500428939454828 1108 12-13 -0.19474564387316917 1108 14-15 0.0660322786740366 1108 16-17 0.04956772669465437 1108 18-19 0.2585155889784332 1108 20-21 0.05449533520428673 1108 22-23 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2311 6 0.40795997707832043 2311 7 -2.7837755542000906 2311 8 -1.0346750228000516 2311 9 -2.9889401583765682 2311 10-11 -1.4479137376858944 2311 12-13 -1.5123829475508543 2311 14-15 -1.8455633380137613 2311 16-17 0.033507504927676734 2311 18-19 0.6588667742286489 2311 20-21 -0.8584692536163132 2311 22-23 -0.025978826170650393 2311 24-25 0.1596311222593343 2311 26-27 0.4230600601002976 2311 28-29 0.3943454342591153 2311 30-31 0.8047728863382275 2311 32-33 0.4249720635740317 2311 34-35 0.26562817613066514 2311 36-37 0.4216624292342743 2311 38-39 0.27665640050855345 2311 40-41 -0.12806220080170405 2311 42-43 0.6240980941633154 2311 44-45 0.7647750578542762 2311 46-47 0.4967825648597781 2311 48-49 1.4315491888143086 2311 50-51 1.0312841677357056 2311 52-53 1.2208405101780393 2311 54-55 1.257635830518165 2311 56-57 1.4021758047560873 2311 58-59 1.640167135366589 2311 60-61 1.7786872046488362 2311 62-63 1.4420556338999226 2311 64-65 1.9730365736710986 2311 66-67 2.3418083572584685 2311 68-69 3.7412867490178705 2311 70-71 4.9559254792138745 2311 72-73 4.195557494227284 2311 74-75 3.3062380126187456 2311 76-77 2.7038184240084426 2311 78-79 2.3229188514192565 2311 80-81 2.1452360883837542 2311 82-83 1.8227167334617036 2311 84-85 1.1604226178170478 2311 86-87 0.9167565161147841 2311 88-89 1.1958771785334399 2311 90-91 1.5540198550578062 2311 92-93 1.591231513428454 2311 94-95 1.9310066947212263 2311 96-97 1.0190806877028358 2311 98-99 0.5548121295144632 2311 100-101 0.8816754606055586 2312 1 -0.25322350713299 2312 2 0.05552720055749916 2312 3 0.023398372300441395 2312 4 0.340250836852654 2312 5 -1.9333407805816947 2312 6 -0.43953782801386865 2312 7 -0.31042661263341387 2312 8 0.27073908314083184 2312 9 -2.801416989905203 2312 10-11 -0.26295617257029846 2312 12-13 -1.6440920491019213 2312 14-15 -4.420351163591757 2312 16-17 -1.2320226872487012 2312 18-19 -0.25945826723071974 2312 20-21 0.007109714289256885 2312 22-23 0.44875046853230316 2312 24-25 0.6164792346386108 2312 26-27 0.4532961346296105 2312 28-29 0.1305760459067642 2312 30-31 0.17558061241373224 2312 32-33 0.14044860244828783 2312 34-35 0.2109652164194138 2312 36-37 0.3374657654941515 2312 38-39 0.8239298372464354 2312 40-41 0.9568758540222113 2312 42-43 -0.051811573186210325 2312 44-45 0.7389824522548238 2312 46-47 0.7802963683911415 2312 48-49 0.2370462134948248 2312 50-51 0.9857959226864388 2312 52-53 1.0771862325465875 2312 54-55 1.0716588526623454 2312 56-57 1.164477043659609 2312 58-59 0.7314070144027802 2312 60-61 0.7065820441766881 2312 62-63 0.7548153646578974 2312 64-65 0.34106896075528326 2312 66-67 1.4189276626920844 2312 68-69 3.784926393930565 2312 70-71 4.098550588959267 2312 72-73 2.178266498226897 2312 74-75 1.697443748644499 2312 76-77 2.3619590932114463 2312 78-79 1.6581398054725192 2312 80-81 0.8702336495972922 2312 82-83 1.2919099829007799 2312 84-85 1.6085740176804748 2312 86-87 1.0555722414098732 2312 88-89 0.8214210279139884 2312 90-91 1.0416356974146481 2312 92-93 1.5485966485372238 2312 94-95 1.2989415303470189 2312 96-97 1.9698220787866347 2312 98-99 1.8607920339140342 2312 100-101 1.5608921223946535 2313 1 -0.106164683603577 2313 2 0.09526209763159343 2313 3 0.058551997613491835 2313 4 0.1878166544569524 2313 5 0.19294961096433383 2313 6 0.23729926196230622 2313 7 -2.3351960219547756 2313 8 -3.2777307720909796 2313 9 -0.49260873139930794 2313 10-11 0.52328168705953 2313 12-13 0.5089682404344487 2313 14-15 0.009051438570601533 2313 16-17 0.2242872510568361 2313 18-19 0.8025874233196433 2313 20-21 -1.9781233197343155 2313 22-23 0.41947614197925276 2313 24-25 0.8785737920877636 2313 26-27 -0.7225483464650111 2313 28-29 -1.0083281138879912 2313 30-31 -0.23668803336233424 2313 32-33 -0.8225194508166318 2313 34-35 -0.9580989824688615 2313 36-37 -1.787511328147044 2313 38-39 -1.9055307943582314 2313 40-41 -0.676469696035813 2313 42-43 0.4275574329832317 2313 44-45 -0.012590451049799611 2313 46-47 -0.05740444402104927 2313 48-49 -2.230686362401574 2313 50-51 -0.40999541565575015 2313 52-53 -0.10050602815831766 2313 54-55 -0.21880904789351519 2313 56-57 -0.2889619643423451 2313 58-59 0.07988908635929093 2313 60-61 0.397239303965339 2313 62-63 0.9614307210808413 2313 64-65 1.11533443018293 2313 66-67 1.8774060696311814 2313 68-69 2.0295510350642765 2313 70-71 4.163467209813216 2313 72-73 4.2523762044146665 2313 74-75 2.238446130905814 2313 76-77 2.6675604614846122 2313 78-79 2.470903228598779 2313 80-81 2.3508854118598315 2313 82-83 2.1248462726814914 2313 84-85 1.8787468843349266 2313 86-87 2.047295410428262 2313 88-89 1.2077291337108242 2313 90-91 1.8777330647104762 2313 92-93 1.987283961468627 2313 94-95 2.5547292036718012 2313 96-97 2.9085399335297737 2313 98-99 3.350957570533666 2313 100-101 3.4610356689098296 2314 1 -0.3088224909125472 2314 2 -0.1512888591997381 2314 3 -0.2006152641618968 2314 4 -0.28440988014732227 2314 5 -0.2484451168898616 2314 6 -3.157438941947813 2314 7 -1.1288640651337971 2314 8 -0.14780042568595775 2314 9 -0.37409412277385456 2314 10-11 -0.680323119349012 2314 12-13 -2.417217578383408 2314 14-15 -0.8675695858443788 2314 16-17 -1.9562480363030517 2314 18-19 -0.6480703011915878 2314 20-21 -0.9139757741696926 2314 22-23 -0.6499238849300113 2314 24-25 -0.05974706983666778 2314 26-27 -2.263264132888253 2314 28-29 -0.8222609028262227 2314 30-31 -0.2603681857101705 2314 32-33 -1.0449193431795791 2314 34-35 -0.8712389830898424 2314 36-37 -0.6186608712070409 2314 38-39 -0.5601710899461487 2314 40-41 -0.6286126049272589 2314 42-43 -0.7482428588787862 2314 44-45 -3.9412446773567105 2314 46-47 -4.717597362744723 2314 48-49 -4.53134563937035 2314 50-51 -4.254459869552935 2314 52-53 -1.9161258209569816 2314 54-55 -2.222648792876491 2314 56-57 -2.256836442975356 2314 58-59 -2.552494142959386 2314 60-61 -1.6446925741977942 2314 62-63 -1.7239482338047445 2314 64-65 -1.93582939548644 2314 66-67 -1.2019710844036524 2314 68-69 -1.5334839159298177 2314 70-71 0.6325060316014408 2314 72-73 0.5522540777548315 2314 74-75 0.27921366591372987 2314 76-77 0.37237100991611527 2314 78-79 -1.3351016771670494 2314 80-81 -1.2236635992247145 2314 82-83 -0.46763879785182993 2314 84-85 0.09060558999932766 2314 86-87 -0.2515630157937814 2314 88-89 -0.6846752090303987 2314 90-91 -0.2594576081246238 2314 92-93 -1.2064627395591003 2314 94-95 -0.3982986060176046 2314 96-97 0.08352259775390891 2314 98-99 0.3493378398333 2314 100-101 0.11189314295791775 2315 1 0.0817785788077714 2315 2 -0.05301125028383069 2315 3 0.3097285071912772 2315 4 -0.21474846783375767 2315 5 -0.43285406107119684 2315 6 0.21357022371746126 2315 7 -1.076662184876497 2315 8 -3.0580051805220805 2315 9 -0.9957571373116849 2315 10-11 -1.2218631396124309 2315 12-13 -0.37782171117951435 2315 14-15 0.2234744517607652 2315 16-17 0.3071234676671608 2315 18-19 0.4527166736676236 2315 20-21 -0.14106161097843284 2315 22-23 0.39284725618353633 2315 24-25 -4.861931278502812 2315 26-27 -0.767222569305865 2315 28-29 0.1055447567953749 2315 30-31 -0.5746279816621964 2315 32-33 0.45243580867829536 2315 34-35 -2.0676469376620723 2315 36-37 -3.5132851731791916 2315 38-39 -0.5007120762565584 2315 40-41 0.4468981040569808 2315 42-43 -1.0457971106636137 2315 44-45 -4.686167040166257 2315 46-47 -3.5447279675843824 2315 48-49 -1.6135656624612302 2315 50-51 -0.13272027812012155 2315 52-53 0.8887562506247413 2315 54-55 -0.500027978098327 2315 56-57 -0.22316028077371186 2315 58-59 0.43715030462667315 2315 60-61 0.27955520507225273 2315 62-63 1.052270516931575 2315 64-65 1.431981212180677 2315 66-67 1.8807201811178977 2315 68-69 4.142943359582073 2315 70-71 4.623581878070656 2315 72-73 -0.8354311794257256 2315 74-75 -0.1804244198995164 2315 76-77 1.0014737643281215 2315 78-79 0.5668798972539157 2315 80-81 1.056890581873752 2315 82-83 0.8869593501654158 2315 84-85 0.7505570520289666 2315 86-87 1.1121360016657498 2315 88-89 1.1419605912570567 2315 90-91 1.559296662509908 2315 92-93 0.11239167009194517 2315 94-95 0.3827268175115286 2315 96-97 0.8129940993679163 2315 98-99 -1.337963354766261 2315 100-101 -2.1033409467195163 2316 1 -0.4686100984507391 2316 2 -0.19807613306124594 2316 3 -0.7198016733655699 2316 4 -0.43945347573118454 2316 5 -0.22781519334534295 2316 6 -0.4900130399326983 2316 7 -0.4527190463463455 2316 8 -0.9785973326481923 2316 9 -5.516646604312829 2316 10-11 -1.6073349721250523 2316 12-13 -0.530319465832811 2316 14-15 -0.120946248787412 2316 16-17 -0.4956654078012619 2316 18-19 -0.8641404603408631 2316 20-21 -4.091578814979798 2316 22-23 -1.677865680110557 2316 24-25 -0.32797234511992457 2316 26-27 -0.735539952934964 2316 28-29 -0.04182741329351103 2316 30-31 0.04284236427604782 2316 32-33 -1.7497460489202652 2316 34-35 -0.8936170822933747 2316 36-37 -0.8984705304215943 2316 38-39 -5.635727096851806 2316 40-41 -5.67925166831132 2316 42-43 -2.9646394408689467 2316 44-45 -2.9462788429473896 2316 46-47 -2.059601453910993 2316 48-49 -2.4175546370346126 2316 50-51 -0.9315914107039731 2316 52-53 -0.9704063124772411 2316 54-55 0.44181754204785406 2316 56-57 -2.203455427727782 2316 58-59 0.10085930769273332 2316 60-61 0.19785555503663588 2316 62-63 0.8663403239866092 2316 64-65 1.4505246789960005 2316 66-67 1.2520540719166213 2316 68-69 3.0471135104074314 2316 70-71 3.827358706092582 2316 72-73 3.0428168307325336 2316 74-75 1.7102659080759572 2316 76-77 -0.2857989910994263 2316 78-79 1.6224560996757909 2316 80-81 1.5739397317390313 2316 82-83 2.116309902985261 2316 84-85 2.1728061151774085 2316 86-87 2.531140925943369 2316 88-89 2.3425629635819973 2316 90-91 2.243871904852501 2316 92-93 1.6520801088791508 2316 94-95 1.8885182635148148 2316 96-97 2.0551818413233818 2316 98-99 1.9760987097139022 2316 100-101 2.0998009901630112 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 32.0 4 79.0 5 205.0 6 490.0 7 946.0 8 1733.0 9 2943.0 10 4381.0 11 5748.0 12 6909.0 13 8360.0 14 10332.0 15 12819.0 16 14375.0 17 14651.0 18 11894.0 19 10034.0 20 9863.0 21 9887.0 22 9934.0 23 10698.0 24 11675.0 25 13500.0 26 16090.0 27 18707.0 28 21399.0 29 23585.0 30 26136.0 31 27769.0 32 28876.0 33 29488.0 34 28548.0 35 22743.0 36 13714.0 37 6755.0 38 1414.0 39 42.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.71676578555056 24.540110515456362 18.121237241830993 14.621886457162091 2 19.24371556670956 27.330472574728542 34.999106967031054 18.426704891530843 3 21.80533931678725 29.680144701895777 33.063009433098266 15.451506548218703 4 15.017640320733106 22.572279495990834 34.0565864833906 28.35349369988545 5 10.7910275871701 42.76480729735484 32.04567257607708 14.398492539397978 6 26.09367594634136 41.12180345772631 15.466353451887874 17.318167144044455 7 22.314034449046506 40.61122673498779 19.62171530883023 17.45302350713548 8 27.393929403037358 35.35549190518385 18.063883909048656 19.186694782730143 9 26.51473002397192 17.529266578288812 20.586545044085895 35.36945835365337 10-11 24.679951732302037 31.134840439538298 23.450934327995952 20.734273500163717 12-13 27.186836736796554 29.710871620863582 26.925366504793242 16.176925137546622 14-15 23.197469554287128 29.036131889065185 29.829014037425505 17.93738451922218 16-17 17.134581921421375 31.984156058247095 30.99894678999908 19.88231523033245 18-19 16.663690210435455 26.516218252087526 39.70226279361024 17.117828743866784 20-21 18.282653442042673 25.40073403989825 41.062045374930456 15.254567143128622 22-23 17.635179921261194 25.90195312991903 41.16168930111583 15.301177647703948 24-25 16.98082319121972 26.34644643464304 41.46774538839592 15.204984985741321 26-27 16.938928544851944 26.46754094231727 41.355604274830135 15.237926238000652 28-29 16.655714658595 26.51034678560471 41.57500560956511 15.258932946235179 30-31 16.497618829563145 26.92989284733034 41.42377965015111 15.148708672955399 32-33 16.750618765125687 27.127441575063475 40.882802107350315 15.239137552460521 34-35 16.715131255080195 26.979702114506175 41.06652623384355 15.23864039657008 36-37 17.031697349713408 26.91290246079219 40.79216450955435 15.263235679940056 38-39 16.939404250833796 27.183132317541936 40.67129764887714 15.206165782747128 40-41 17.040856539291653 27.31765546884666 40.358663226823445 15.282824765038239 42-43 16.696777682181192 27.76449481659012 39.85549003816099 15.683237463067703 44-45 17.224755954078926 28.024067206712424 38.77629343478953 15.974883404419119 46-47 17.35355407670303 28.497913275189912 37.6706812586565 16.477851389450553 48-49 17.75315832161039 28.868915333611323 36.66574257788694 16.71218376689135 50-51 17.94386549346518 29.5092784260985 35.78634933254214 16.760506747894183 52-53 18.05004822020484 29.875681210613486 35.25240585606326 16.821864713118416 54-55 17.683370267933324 29.97967165426924 35.11707409252547 17.21988398527197 56-57 18.110646464461404 30.140248985462108 34.50470855509651 17.244395994979982 58-59 18.374649748483055 29.591744427179172 34.80289155743552 17.230714266902247 60-61 18.427922399106013 29.7194157949695 34.95051477458003 16.90214703134446 62-63 18.734917138709662 29.498298813519742 35.12709671806097 16.63968732970963 64-65 18.681000383529963 29.65373541011947 34.94301431654388 16.72224988980669 66-67 18.94107188942735 29.73096665041723 34.5990307687896 16.72893069136581 68-69 21.512157449446413 27.965423735857293 34.78451594730906 15.737902867387236 70-71 21.86514847683743 27.83348020270286 34.69019814643917 15.611173174020543 72-73 20.095170772917708 29.116739366562644 34.03021786980685 16.757871990712804 74-75 18.60696619997755 30.324826623961293 33.840688784569245 17.227518391491916 76-77 18.36826728662672 30.25449421311968 34.30553993670349 17.071698563550115 78-79 17.975160954586933 30.36376938868744 34.19210128889617 17.46896836782946 80-81 17.717890302121376 30.738531637454347 33.884188714238284 17.65938934618599 82-83 18.083957921440994 30.775298251076975 33.38969604205566 17.751047785426366 84-85 17.903498709820703 30.607013904629767 33.2932656532978 18.196221732251736 86-87 17.918030910131655 30.485403548941044 33.19015455065827 18.40641099026903 88-89 18.034930098590067 30.403449965427075 33.20843579281891 18.353184143163947 90-91 17.783619049363576 30.550397376923062 33.32493777748266 18.341045796230702 92-93 17.713857214818884 30.580322388606497 33.33699684022531 18.368823556349316 94-95 17.735837813122046 30.26656414052804 33.251931246368706 18.745666799981205 96-97 17.719245803369674 30.274262690676533 33.083660027418254 18.92283147853554 98-99 17.86760706904744 30.20912884924747 32.79367060345667 19.129593478248424 100-101 17.928478440828634 30.209975646534698 33.02041363107076 18.841132281565905 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5862.0 1 4220.0 2 2434.5 3 2411.0 4 2255.5 5 2085.0 6 3216.0 7 6247.0 8 8686.0 9 9097.5 10 7757.0 11 5591.5 12 4345.5 13 3710.0 14 3306.0 15 2964.0 16 2692.5 17 2610.0 18 2655.0 19 2630.0 20 2660.0 21 2828.5 22 2981.5 23 3203.5 24 3473.0 25 3771.5 26 4313.0 27 4887.5 28 5391.5 29 5976.5 30 6756.0 31 7495.0 32 8223.5 33 9069.0 34 9863.5 35 10497.5 36 11053.5 37 11581.0 38 11837.5 39 11808.5 40 11737.5 41 11779.5 42 11853.0 43 11937.0 44 12066.5 45 12151.0 46 12014.0 47 11971.5 48 12394.0 49 12349.0 50 11434.0 51 10386.5 52 9371.5 53 8623.0 54 8043.0 55 7334.0 56 6629.0 57 5867.0 58 5191.5 59 4748.0 60 4282.0 61 3652.0 62 2916.5 63 2219.5 64 1614.5 65 1182.0 66 878.5 67 658.5 68 498.5 69 351.5 70 249.0 71 190.0 72 154.5 73 136.0 74 110.5 75 84.0 76 62.0 77 47.0 78 28.5 79 15.0 80 11.5 81 8.0 82 4.0 83 2.5 84 1.5 85 1.5 86 2.5 87 2.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01831665373052997 2 0.010761034066686357 3 2.2895817163162463E-4 4 0.059758082795854026 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.006868745148948739 12-13 0.0 14-15 0.0 16-17 2.2895817163162463E-4 18-19 0.0 20-21 0.0 22-23 1.1447908581581231E-4 24-25 0.002175102630500434 26-27 0.004235726175185055 28-29 0.0016027072014213723 30-31 2.2895817163162463E-4 32-33 9.158326865264985E-4 34-35 0.003663330746105994 36-37 0.07704442475404169 38-39 0.08070775550014768 40-41 0.07029015869090875 42-43 0.07338109400793569 44-45 0.0724652613214092 46-47 0.07132047046325107 48-49 0.06754266063132927 50-51 0.06674130703061858 52-53 0.050828714102220666 54-55 0.04086903363624499 56-57 0.024956440707847083 58-59 0.025299877965294523 60-61 0.014882281156055601 62-63 0.0016027072014213723 64-65 0.006525307891501301 66-67 0.19427100862943347 68-69 0.8314616002802447 70-71 1.0083317878656748 72-73 0.4014781539560538 74-75 0.06456620440011815 76-77 0.23674274946709986 78-79 0.01591259292839791 80-81 0.003663330746105994 82-83 0.00148822811560556 84-85 0.0 86-87 0.002518539887947871 88-89 6.868745148948739E-4 90-91 0.005037079775895742 92-93 0.004808121604264117 94-95 0.10291669814841527 96-97 0.04361653169582449 98-99 0.015683634756766287 100-101 0.015454676585134662 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 436761.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.02592320774804 #Duplication Level Percentage of deduplicated Percentage of total 1 85.45437078218076 53.00386239901972 2 8.817909749322641 10.93877985928678 3 2.5807812389280063 4.802260168252361 4 1.1002832023815892 2.7298432567078215 5 0.5633896351099238 1.7472381121684664 6 0.33442430413087 1.244578572409555 7 0.21669559940650862 0.9408521225771526 8 0.16113812720724144 0.7995792883197353 9 0.10945012017313925 0.6109870274044138 >10 0.5957862365661282 6.554224098578406 >50 0.0401314604332687 1.7046347935275592 >100 0.020065730212057962 2.216762769231685 >500 0.0018579379825979594 0.7002001760164129 >1k 0.0022295255791175512 2.9911777104479946 >5k 7.431751930391837E-4 3.912178336965962 >10k+ 7.431751930391837E-4 5.102841309086037 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11290 2.584937757721042 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10850 2.484196162203127 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9545 2.185405748223857 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7429 1.7009302570513394 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3484 0.7976902699645803 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2613 0.5982677024734351 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2606 0.5966649952720138 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1498 0.34297934110417366 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1454 0.3329051815523822 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1323 0.30291166106863937 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 668 0.15294405864992525 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 611 0.13989344286692262 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 606 0.13874865200876452 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 604 0.13829073566550126 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 549 0.1256980362257619 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 1.1447908581581231E-4 0.0 22-23 0.0 0.0 0.0 3.4343725744743694E-4 0.0 24-25 0.0 0.0 0.0 9.158326865264985E-4 0.0 26-27 0.0 0.0 0.0 0.0017171862872371847 0.0 28-29 0.0 0.0 0.0 0.002175102630500434 0.0 30-31 0.0 0.0 0.0 0.0030909353170269324 0.0 32-33 0.0 0.0 0.0 0.0053805170333431785 0.0 34-35 0.0 0.0 0.0 0.00938728503689661 0.0 36-37 0.0 0.0 0.0 0.017171862872371848 0.0 38-39 0.0 0.0 0.0 0.02690258516671589 0.0 40-41 0.0 0.0 0.0 0.03766361923340225 0.0 42-43 0.0 0.0 0.0 0.04979840232987835 0.0 44-45 0.0 0.0 0.0 0.0642227671426707 0.0 46-47 0.0 0.0 0.0 0.08150910910085837 0.0 48-49 0.0 0.0 0.0 0.10371805174912595 0.0 50-51 0.0 0.0 0.0 0.12798761794207816 0.0 52-53 0.0 0.0 0.0 0.1515703096201355 0.0 54-55 0.0 0.0 0.0 0.17629779215635094 0.0 56-57 0.0 0.0 0.0 0.2034293354946985 0.0 58-59 0.0 0.0 0.0 0.2325070232919148 0.0 60-61 0.0 0.0 0.0 0.2657059581785004 0.0 62-63 0.0 0.0 0.0 0.29821801855019103 0.0 64-65 0.0 0.0 0.0 0.3255785200601702 0.0 66-67 0.0 0.0 0.0 0.36427245106591477 0.0 68-69 0.0 0.0 0.0 0.40869033636244995 0.0 70-71 0.0 0.0 0.0 0.4725696662476732 0.0 72-73 0.0 0.0 0.0 0.5457218020839774 0.0 74-75 0.0 0.0 0.0 0.6236820595245454 0.0 76-77 0.0 0.0 0.0 0.7172114726360641 0.0 78-79 0.0 0.0 0.0 0.8249362923887436 0.0 80-81 0.0 0.0 0.0 0.9500619331854263 0.0 82-83 0.0 0.0 0.0 1.0785074674707678 0.0 84-85 0.0 0.0 0.0 1.2100439370731362 0.0 86-87 0.0 0.0 0.0 1.3586377904620606 0.0 88-89 0.0 0.0 0.0 1.5113528909403542 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCACGC 35 3.4542609E-6 67.72149 3 TATCACG 40 7.622435E-6 59.26309 2 GGTATCA 4345 0.0 47.901493 1 TCACGCA 50 2.8557539E-5 47.410473 4 CCCGCTG 95 8.8521665E-7 34.914032 9 CGTATCA 55 0.0026018396 34.480343 2 GTATCAA 6730 0.0 33.602966 1 TCCCGCT 110 2.7811384E-6 30.153028 8 CACGCAG 80 4.5542463E-4 29.61458 5 GTGTAAC 115 3.9144234E-6 28.85855 1 GGTACTC 100 5.0140974E-5 28.443024 3 GTGGTAC 120 5.4510347E-6 27.656107 1 TACTCCC 120 5.475369E-6 27.640274 5 TGGTATC 2130 0.0 27.37788 2 CCGTCAG 35 0.008383208 27.147678 92-93 GATATAG 140 5.888487E-7 27.091698 1 GTGGTAT 2165 0.0 26.935278 1 CTCCCGC 125 7.5194403E-6 26.534664 7 TATCAAC 9375 0.0 26.09472 1 ATCAACG 9690 0.0 25.050735 2 >>END_MODULE