FastQCFastQC Report
Fri 27 May 2016
SRR939507_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939507_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences833090
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73990.8881393366863124No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71370.8566901535248292No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56950.6835996110864373No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA48400.5809696431357957No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40560.48686216375181557No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23960.2876039803622658No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA16670.20009842874119244No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13930.16720882497689324No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA13860.1663685796252506No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11350.13623978201634876No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT11230.13479936141353274No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10180.12219568113889256No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG8250.056.8427622
GTACCTG9650.048.8489231
ACCTGGG11000.041.372063
AACGCGT650.005241670629.8876046
GTACATG71100.029.4822861
TACATGG70300.028.4201162
GGACCGC700.00752802427.7527736
ACATGGG71150.027.3355223
CCTGGGA10050.026.2003544
GGTATCA83600.025.0140611
GTATCAA112400.024.94021
CATGGGG18150.024.7948654
CATGGGA49650.024.5884674
CCTGGGG6450.023.7520314
CTTAGGG2103.1472155E-822.8116653
ATGGGAG16150.022.7451425
ATGGGAC5950.022.7451425
GAGTACT62600.022.72993512-13
GTCCTAC1854.093028E-621.6856631
ACGCAGG2506.521077E-921.5893338
Produced by FastQC (version 0.11.5)