FastQCFastQC Report
Fri 27 May 2016
SRR939507_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939507_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences833090
Sequences flagged as poor quality0
Sequence length101
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98721.1849860159166477No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98431.181504999459842No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89681.0764743305045072No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA78390.9409547587895665No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64890.7789074409727641No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34990.42000264077110516No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA23490.28196233300123635No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23090.27716093099184963No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA20520.24631192308153982No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20270.24331104682567312No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT16610.19937821843978443No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16410.19697751743509104No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT12570.15088405814497832No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10460.12555666254546327No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT10320.12387617184217792No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG10450.049.8743552
GTACCTG12200.043.4995881
ACCTGGG12400.041.262053
TATCACG500.001635653237.9045072
TATAACG1203.8853614E-935.5354772
TAACGCA1255.7953002E-934.1099624
ATAACGC1401.7491402E-830.4553263
GTACACG1303.1011405E-729.1590631
GTACATG79750.028.043891
TACATGG78400.027.8602962
CCTGGGA11250.027.372194
GTACCGG700.00849138227.0762751
ACATGGG79250.026.7212643
CATGGGT7350.026.4268424
CATGGGA53800.024.479994
GGTATCA93000.023.131291
GTATCAA121400.022.8720911
CATGGGG20900.022.4407654
ATGGGAC7650.022.2940925
GCAACGC550.002672040121.86877468-69
Produced by FastQC (version 0.11.5)