##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939507_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 833090 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.032892004465303 31.0 31.0 33.0 30.0 34.0 2 31.2414324982895 31.0 31.0 34.0 30.0 34.0 3 31.21053187530759 31.0 31.0 34.0 28.0 34.0 4 35.15848347717534 35.0 35.0 37.0 33.0 37.0 5 34.834637314095716 35.0 35.0 37.0 32.0 37.0 6 34.82537420926911 35.0 35.0 37.0 32.0 37.0 7 34.726261268290344 35.0 35.0 37.0 31.0 37.0 8 34.75434226794224 35.0 35.0 37.0 32.0 37.0 9 36.08098644804283 38.0 35.0 39.0 31.0 39.0 10-11 35.93774622189679 37.0 35.0 39.0 30.0 39.0 12-13 35.903832719153996 37.0 35.0 39.0 30.0 39.0 14-15 36.68363862247776 38.0 35.5 40.0 31.0 41.0 16-17 36.71539629571835 38.0 35.5 40.0 31.0 41.0 18-19 36.7711789842634 38.5 35.5 40.0 31.0 41.0 20-21 36.684074349710116 38.5 35.0 40.0 30.0 41.0 22-23 36.47872078646965 38.0 35.0 40.0 30.0 41.0 24-25 36.22536760734134 38.0 34.5 40.0 30.0 41.0 26-27 35.8134007130082 38.0 34.0 40.0 28.0 41.0 28-29 35.51686372420747 38.0 34.0 40.0 27.0 41.0 30-31 35.21625454632753 37.5 34.0 40.0 27.0 41.0 32-33 34.85312271183186 37.0 33.0 40.0 27.0 41.0 34-35 34.59964589660181 37.0 33.0 40.0 25.5 41.0 36-37 34.429362973988404 37.0 33.0 40.0 24.5 41.0 38-39 34.35277160930992 37.0 33.0 40.0 24.0 41.0 40-41 34.10401517243035 37.0 33.0 40.0 24.0 41.0 42-43 33.7687488746714 37.0 33.0 40.0 22.5 41.0 44-45 33.497037534960214 37.0 33.0 40.0 22.0 41.0 46-47 33.039532343444286 37.0 32.0 40.0 19.5 41.0 48-49 32.641205031869305 36.5 31.0 40.0 16.5 41.0 50-51 32.36944747866377 36.0 31.0 40.0 14.5 41.0 52-53 32.00855609838073 36.0 31.0 39.0 10.0 41.0 54-55 31.823556878608553 35.5 31.0 39.0 9.0 40.5 56-57 31.476707798677214 35.0 30.0 39.0 8.0 40.0 58-59 31.09418550216663 35.0 29.5 39.0 7.5 40.0 60-61 30.858223601291577 35.0 29.0 39.0 6.0 40.0 62-63 30.503446806467487 35.0 28.0 38.0 2.0 40.0 64-65 30.10434286811749 34.5 28.0 38.0 2.0 40.0 66-67 28.553404194024655 33.5 25.0 37.5 2.0 39.5 68-69 25.113158242206723 30.0 14.0 35.0 2.0 39.0 70-71 24.563098224681607 29.5 11.0 35.0 2.0 38.0 72-73 26.305226326087215 31.0 21.0 35.0 2.0 38.0 74-75 26.862942179116303 32.0 22.5 35.0 2.0 38.0 76-77 26.82871658524289 31.5 25.0 34.5 2.0 36.5 78-79 27.61762714712696 33.0 26.0 35.0 2.0 37.0 80-81 27.473220180292646 33.0 26.0 35.0 2.0 37.0 82-83 27.071694534804163 33.0 25.5 35.0 2.0 36.0 84-85 26.74925338198754 33.0 25.0 35.0 2.0 36.0 86-87 26.313748814653877 32.0 24.5 35.0 2.0 35.5 88-89 25.818561019817786 32.0 22.5 34.5 2.0 35.0 90-91 25.3860879376778 32.0 20.5 34.0 2.0 35.0 92-93 24.8861125448631 31.5 19.0 34.0 2.0 35.0 94-95 23.620979726080016 31.0 7.0 34.0 2.0 35.0 96-97 22.857456577320576 30.0 2.0 34.0 2.0 35.0 98-99 22.57806659544587 30.0 2.0 34.0 2.0 35.0 100-101 21.457425968382765 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.4038943548518894 1101 2 0.3695565078164549 1101 3 0.3957957080564434 1101 4 0.35179097615146304 1101 5 0.45971516103998766 1101 6 0.6016708707290235 1101 7 0.5348229505087048 1101 8 0.7242648292872502 1101 9 0.7648332491924208 1101 10-11 0.9426923464799515 1101 12-13 0.7750012088040137 1101 14-15 0.5763878931795645 1101 16-17 0.6728382259338659 1101 18-19 0.8341527033919363 1101 20-21 0.9312262289145394 1101 22-23 0.9243109972590631 1101 24-25 0.8185379648093161 1101 26-27 1.1653764399375177 1101 28-29 0.8724595568794271 1101 30-31 0.892566590696326 1101 32-33 0.8651219173954061 1101 34-35 1.3124637158216075 1101 36-37 1.173802647957011 1101 38-39 1.1877253332012074 1101 40-41 1.3230965519193774 1101 42-43 1.2331557467725744 1101 44-45 1.087046097362986 1101 46-47 1.1579683542512882 1101 48-49 1.213601635143128 1101 50-51 0.82326390055443 1101 52-53 1.0229157986724893 1101 54-55 0.81438098006657 1101 56-57 0.6757515401853382 1101 58-59 0.7334617905838492 1101 60-61 0.46714398625811526 1101 62-63 0.5228553872370156 1101 64-65 0.539418976290289 1101 66-67 -2.3048742205492765 1101 68-69 -7.145753984858995 1101 70-71 -10.568197529901248 1101 72-73 -10.545650249805583 1101 74-75 -9.039319870754948 1101 76-77 -3.9307922221176774 1101 78-79 -0.7322297111877738 1101 80-81 -0.19317949666339373 1101 82-83 -0.010426618182208358 1101 84-85 -0.11279640086771536 1101 86-87 -0.23650645845245322 1101 88-89 -0.08414505944254458 1101 90-91 -0.4837006719182888 1101 92-93 -0.7344763103363441 1101 94-95 -4.29229445744204 1101 96-97 -1.4923688218549493 1101 98-99 -1.2165641522964137 1101 100-101 -1.1455405396908702 1102 1 0.5623488769909066 1102 2 0.36194364161549686 1102 3 0.4195522690114295 1102 4 0.3669494167073708 1102 5 0.5868243795150292 1102 6 0.5729651849489343 1102 7 0.571938847382043 1102 8 0.677493187340815 1102 9 0.8648555951639239 1102 10-11 0.6572936613372278 1102 12-13 0.6987017778644926 1102 14-15 0.7495253691512929 1102 16-17 0.6824362015920045 1102 18-19 0.7020772197159246 1102 20-21 0.9875488691926506 1102 22-23 0.9623931034684503 1102 24-25 1.046110735227309 1102 26-27 0.9841575941577361 1102 28-29 0.8947166523602093 1102 30-31 0.7662921720089955 1102 32-33 0.7377708291719856 1102 34-35 0.9662084435689593 1102 36-37 1.2337349244274591 1102 38-39 1.1075763008071675 1102 40-41 1.2530038415307843 1102 42-43 1.4046801229263792 1102 44-45 1.2175472057739682 1102 46-47 1.0810271114597683 1102 48-49 1.120022800841042 1102 50-51 1.122572318129535 1102 52-53 1.188876535784825 1102 54-55 1.1990243459793142 1102 56-57 1.0681671143117484 1102 58-59 1.0603179663247566 1102 60-61 0.836809202975946 1102 62-63 0.7579235805398277 1102 64-65 0.7936685198484241 1102 66-67 -1.991826839471745 1102 68-69 -7.268044853228528 1102 70-71 -11.119320922832301 1102 72-73 -8.67110193045118 1102 74-75 -6.624758931005278 1102 76-77 -3.065318144714279 1102 78-79 -0.5910615755274122 1102 80-81 -0.25611133891099414 1102 82-83 0.03240718111541696 1102 84-85 -0.19171894414399304 1102 86-87 -0.19833674587751204 1102 88-89 -0.11938122842887822 1102 90-91 -0.18465951724100194 1102 92-93 -0.4550316839076629 1102 94-95 -3.6031948477347484 1102 96-97 -1.0495935537667727 1102 98-99 -0.16477609367886714 1102 100-101 -0.19082668239529 1103 1 0.24355693068218187 1103 2 0.19975124787947962 1103 3 0.327829673933131 1103 4 0.2763453898617385 1103 5 0.40673489405641305 1103 6 0.4096542229802509 1103 7 0.5629903015207489 1103 8 0.3441598540074793 1103 9 0.750761635432383 1103 10-11 0.6886135718517679 1103 12-13 0.44310893670117935 1103 14-15 0.761829619710575 1103 16-17 0.4363511904063202 1103 18-19 0.678587286830016 1103 20-21 0.45566967456177565 1103 22-23 0.5597085397100656 1103 24-25 0.602598430976748 1103 26-27 0.4880725829720518 1103 28-29 0.6061260483333655 1103 30-31 0.5901176753647022 1103 32-33 0.8602540506484928 1103 34-35 0.9762755576629161 1103 36-37 1.034629779013592 1103 38-39 0.8777105289950811 1103 40-41 0.9549054075263115 1103 42-43 0.9164250893693335 1103 44-45 0.9535651028656886 1103 46-47 0.9193940018400824 1103 48-49 0.8029087068813112 1103 50-51 0.7383441302100735 1103 52-53 0.6659458870152442 1103 54-55 0.8276596927354696 1103 56-57 0.9714109621864679 1103 58-59 0.6934275860115555 1103 60-61 0.7081738562197977 1103 62-63 0.9134045648798725 1103 64-65 0.6862859695128556 1103 66-67 -2.5729230363397484 1103 68-69 -7.841311072467899 1103 70-71 -10.95992494967794 1103 72-73 -8.108462109422092 1103 74-75 -6.155519557403487 1103 76-77 -3.0586067353183033 1103 78-79 -0.5396074368249515 1103 80-81 0.06547702797938726 1103 82-83 0.3310648992362246 1103 84-85 0.006826917153546219 1103 86-87 0.24126056955872954 1103 88-89 0.2447127313026698 1103 90-91 0.021716321685172346 1103 92-93 0.3100689872332758 1103 94-95 1.1468051522652516 1103 96-97 0.9381021956739453 1103 98-99 0.4935013112428308 1103 100-101 0.3953030715196988 1104 1 0.1940965476647527 1104 2 0.20976181896387658 1104 3 0.17896668010369154 1104 4 0.09642307504009295 1104 5 0.3778414161696446 1104 6 0.36606447939050923 1104 7 0.4070348475787142 1104 8 0.37374905675104486 1104 9 0.487249085342647 1104 10-11 0.46054980117055067 1104 12-13 0.3812447382598023 1104 14-15 0.5252266253894646 1104 16-17 0.503762749764185 1104 18-19 0.44098372707880884 1104 20-21 0.5270183482594462 1104 22-23 0.5486826528452511 1104 24-25 0.5657950777304492 1104 26-27 0.8327661444441361 1104 28-29 0.6382277274149075 1104 30-31 0.5823983253634708 1104 32-33 0.35124158660247673 1104 34-35 0.4809747734359604 1104 36-37 0.5062882100679928 1104 38-39 0.490773193400031 1104 40-41 0.6539736770643785 1104 42-43 0.5578760321625609 1104 44-45 0.604364080174733 1104 46-47 0.46348156975315646 1104 48-49 0.330454986105444 1104 50-51 0.4938361773128648 1104 52-53 0.7562376981124324 1104 54-55 0.7050203633847296 1104 56-57 0.6157148685709117 1104 58-59 0.5468238541729242 1104 60-61 0.6870882304361849 1104 62-63 0.8212812519511914 1104 64-65 0.7267117629587823 1104 66-67 -2.0230004756789377 1104 68-69 -6.596262273392256 1104 70-71 -8.36962822179732 1104 72-73 -7.519019672664221 1104 74-75 -6.034129091046577 1104 76-77 -3.0193990749697086 1104 78-79 -0.5615682500609331 1104 80-81 0.0746505166830751 1104 82-83 0.19346256868087153 1104 84-85 -0.07979943188890815 1104 86-87 0.2592744348880345 1104 88-89 0.2904929019150515 1104 90-91 0.23058005303212425 1104 92-93 0.06293547197895677 1104 94-95 1.1317204606704934 1104 96-97 1.3509018121648353 1104 98-99 0.8195860765893386 1104 100-101 0.7039025257567317 1105 1 0.43242495934117997 1105 2 0.3362830950446245 1105 3 0.4231962390909594 1105 4 0.29188708239837524 1105 5 0.3925482951937056 1105 6 0.5117100232739418 1105 7 0.4766141669377717 1105 8 0.6217594164354168 1105 9 0.6238928380657001 1105 10-11 0.7465339711855563 1105 12-13 0.6813282450613727 1105 14-15 0.8400012780495771 1105 16-17 0.9589849504709704 1105 18-19 0.9616909933807847 1105 20-21 0.8042367245967625 1105 22-23 0.9158160700888018 1105 24-25 1.0816819441679755 1105 26-27 1.1054594203312433 1105 28-29 1.1703451694442464 1105 30-31 0.9759695118861984 1105 32-33 1.1967891007623237 1105 34-35 1.3477560098207277 1105 36-37 1.1298891685808456 1105 38-39 0.9704568357488768 1105 40-41 1.0464616956537185 1105 42-43 1.3529672845225278 1105 44-45 1.1705377743219998 1105 46-47 0.8124746471603288 1105 48-49 0.878138580044542 1105 50-51 0.8320919743792317 1105 52-53 1.0931428634316909 1105 54-55 0.9285390253395143 1105 56-57 0.9197473999162185 1105 58-59 0.9786883553105561 1105 60-61 1.04609849330107 1105 62-63 1.2039021552769427 1105 64-65 1.0504809453182737 1105 66-67 1.84599308582564 1105 68-69 -5.263329127243249 1105 70-71 -4.537954939132845 1105 72-73 -1.259446646571373 1105 74-75 1.1224618950598284 1105 76-77 1.1177771848937788 1105 78-79 1.0888492839469102 1105 80-81 1.0978194182660737 1105 82-83 1.0931757409711373 1105 84-85 0.9166823902537047 1105 86-87 0.9465535654491006 1105 88-89 1.0742308599922197 1105 90-91 0.9681593239814426 1105 92-93 0.9224395141922521 1105 94-95 1.7734448156107092 1105 96-97 1.800303951875346 1105 98-99 1.5331020760084257 1105 100-101 1.3590666347754663 1106 1 0.10768146221429475 1106 2 0.12494385022307952 1106 3 0.0870641560468286 1106 4 -0.01347527955279304 1106 5 0.25772434139019396 1106 6 0.13044736338753893 1106 7 0.2354943837552952 1106 8 0.2899074963821562 1106 9 0.29182136194064867 1106 10-11 0.2866519412598265 1106 12-13 0.2864194670679723 1106 14-15 0.416301734631773 1106 16-17 0.2696467585742397 1106 18-19 0.3622320928609284 1106 20-21 0.44238352970179307 1106 22-23 0.5150737108761803 1106 24-25 0.5032113056611465 1106 26-27 0.32235544046429965 1106 28-29 0.5483345552066297 1106 30-31 0.4005437383641919 1106 32-33 0.5479649061553786 1106 34-35 0.5700104965552839 1106 36-37 0.4437038490913565 1106 38-39 0.45073397217109346 1106 40-41 0.295602948114869 1106 42-43 0.5454506761680662 1106 44-45 0.7137041506080521 1106 46-47 0.5790381460814373 1106 48-49 0.45086003657480234 1106 50-51 0.06691545601915294 1106 52-53 0.6327503066186075 1106 54-55 0.4767439773913047 1106 56-57 -0.03509723465276693 1106 58-59 0.2343596302939126 1106 60-61 0.49117701410696313 1106 62-63 0.5058177229828402 1106 64-65 0.4812157263051908 1106 66-67 1.093258078200197 1106 68-69 -5.144480828684767 1106 70-71 -6.068537565401197 1106 72-73 -2.262279628142828 1106 74-75 0.31166611053640736 1106 76-77 0.5348404689804482 1106 78-79 0.42888202849223944 1106 80-81 0.3619713416244821 1106 82-83 0.4490306875930443 1106 84-85 0.30443080732525374 1106 86-87 0.35887927806200537 1106 88-89 0.5573291738960187 1106 90-91 0.3918311277880271 1106 92-93 0.42178733822632 1106 94-95 1.51501551502664 1106 96-97 1.5669713267874066 1106 98-99 1.3318753949879856 1106 100-101 0.9241880280358075 1107 1 -0.14743270451002743 1107 2 0.08960077955028822 1107 3 -0.132170427409406 1107 4 -0.19519889302581817 1107 5 -0.024449724173585707 1107 6 0.017619492893430788 1107 7 0.04962068755980198 1107 8 -0.047982552523031075 1107 9 0.09028319732353651 1107 10-11 -0.09653518632213576 1107 12-13 0.04593740685960768 1107 14-15 -0.019781650592761935 1107 16-17 0.02540911019876546 1107 18-19 0.06621551680662918 1107 20-21 0.07446212758317472 1107 22-23 0.01082470105751554 1107 24-25 0.0683332530319305 1107 26-27 0.015579480598354678 1107 28-29 -0.046148711548163135 1107 30-31 -0.004356369545241989 1107 32-33 0.2787290481782705 1107 34-35 -0.03487528816721408 1107 36-37 0.21676448351142596 1107 38-39 0.10228178673195032 1107 40-41 0.16939624996147273 1107 42-43 0.37325380274983644 1107 44-45 0.5326281151205308 1107 46-47 0.3932178583795931 1107 48-49 0.6965427488523233 1107 50-51 0.46052877124214575 1107 52-53 0.5048600270858365 1107 54-55 0.4517833965010958 1107 56-57 0.2984224214936404 1107 58-59 0.27082731390684955 1107 60-61 0.1522612793578162 1107 62-63 0.3214704172592704 1107 64-65 0.32412131829585533 1107 66-67 1.203073224120697 1107 68-69 -4.954026140329397 1107 70-71 -5.41900410073152 1107 72-73 -2.2054906196108206 1107 74-75 0.27588484744980946 1107 76-77 0.2552403286617668 1107 78-79 0.3558362027908579 1107 80-81 0.3045535694626871 1107 82-83 0.25963931379306615 1107 84-85 0.2867058634952073 1107 86-87 0.4689772462329742 1107 88-89 0.5654757899217273 1107 90-91 0.3245740545006477 1107 92-93 0.1456654260565884 1107 94-95 0.9728950278926263 1107 96-97 1.0564753439558068 1107 98-99 0.8040471421376125 1107 100-101 0.6900312328367448 1108 1 -0.05771900172521782 1108 2 -0.11402872345952275 1108 3 0.04902253384201671 1108 4 -0.14313372978683248 1108 5 -0.0681004038625801 1108 6 0.06217167979760063 1108 7 0.11074712207378923 1108 8 -0.13971278460495284 1108 9 0.027060321515513408 1108 10-11 0.14192845559220046 1108 12-13 0.2172025511194846 1108 14-15 0.13324545330906545 1108 16-17 -0.021030373320009232 1108 18-19 0.1818363144032773 1108 20-21 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84-85 0.2920596145764378 2316 86-87 -0.019387847711257677 2316 88-89 0.5699166277954717 2316 90-91 0.5064020656651635 2316 92-93 0.7624703858839474 2316 94-95 1.4207530815928777 2316 96-97 1.4492988875304142 2316 98-99 0.6913932064673389 2316 100-101 0.8962080548732878 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 14.0 4 30.0 5 146.0 6 359.0 7 739.0 8 1296.0 9 1985.0 10 3227.0 11 4951.0 12 6347.0 13 7432.0 14 8381.0 15 10483.0 16 12052.0 17 13698.0 18 11764.0 19 8788.0 20 8403.0 21 8406.0 22 8938.0 23 10138.0 24 12043.0 25 14986.0 26 18458.0 27 23119.0 28 28252.0 29 33759.0 30 40235.0 31 46800.0 32 54860.0 33 64249.0 34 76462.0 35 88109.0 36 88030.0 37 77078.0 38 36312.0 39 2760.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.06718100548196 20.413632139692695 14.697555810083635 18.821631044741714 2 15.665174733829257 23.596510606009673 40.4232559032997 20.31505875686137 3 18.98894477187339 31.39180640747098 30.649029516618853 18.970219304036778 4 11.814929959548188 19.173438644084072 37.6895653530831 31.32206604328464 5 11.098200674596983 40.27752103614255 34.163415717389476 14.460862571870987 6 25.554862019709756 37.74646196689434 20.934352831026658 15.764323182369253 7 24.710655511409332 35.85867073185371 23.21633917103794 16.214334585699024 8 25.50276680790791 33.76513942071085 21.819971431658043 18.9121223397232 9 26.943787585975105 17.786673708722947 22.78841421695135 32.4811244883506 10-11 23.23032325439028 28.59715036790743 28.317468700860655 19.855057676841636 12-13 25.5574427732898 27.05199918376166 29.115401697295614 18.27515634565293 14-15 22.336422235292705 27.36853161123048 30.094467584534684 20.20057856894213 16-17 18.630887290283006 30.62801364083223 30.74162722500771 19.99947184387705 18-19 18.247494826402395 28.488517254128077 34.91716241975522 18.34682549971431 20-21 20.05103896462443 27.17782853932773 34.69005112298926 18.08108137305858 22-23 19.38962176955671 27.516954950845644 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19.29959686867291 48-49 19.168950737371322 29.183754639737664 32.41880383988234 19.228490783008677 50-51 19.179682947080543 29.597693898419543 32.26277205618179 18.95985109831813 52-53 19.258101117028847 29.897475047496403 31.914337696254886 18.930086139219863 54-55 18.84347384263903 29.646470531002894 32.05794702583364 19.452108600524433 56-57 19.25365857758874 30.078329044428887 31.37971033984214 19.28830203814023 58-59 19.356360669966918 29.530539343418972 31.640348081225355 19.47275190538875 60-61 19.285233243658862 29.640113659690787 32.00532284610204 19.06933025054831 62-63 19.533317368317125 29.453474281619286 31.980388237984375 19.032820112079214 64-65 19.21693681884259 29.698253043938593 31.892241632845124 19.192568504373693 66-67 18.88152978328472 29.787503314610802 31.8793240604585 19.451642841645974 68-69 19.18149253045082 29.77698133777318 31.69765341267618 19.34387271909982 70-71 18.858430982901712 29.931658925542926 31.748840042408162 19.461070049147207 72-73 19.23711615680157 29.83463086537443 31.048689950024556 19.879563027799442 74-75 18.731461385286433 30.35268972572317 31.27109307428909 19.644755814701305 76-77 19.01546510566982 30.10539369870776 31.183881330945095 19.695259864677325 78-79 18.811395658442365 30.118280615448146 31.285480883389216 19.784842842720273 80-81 18.751744291777026 30.658542543313956 30.90209819169834 19.687614973210678 82-83 18.796108463671395 30.679998559579396 30.632464679686468 19.891428297062742 84-85 18.66310962801138 30.322654215030788 30.615419702553147 20.398816454404685 86-87 18.810269029363663 30.301378906777497 30.761114528785992 20.127237535072847 88-89 18.97644052273303 30.01669701578354 30.66867286932995 20.338189592153483 90-91 18.64957944155216 30.19125896245044 30.918878494800488 20.240283101196916 92-93 18.56770054965171 30.61190315854673 30.703791864578495 20.116604427223063 94-95 18.15518867470575 30.457539770376002 30.751486877941275 20.63578467697697 96-97 18.112199476429712 30.253929183268852 30.603838232180326 21.030033108121113 98-99 18.417692759733654 30.288178435448426 30.196698934599958 21.097429870217958 100-101 18.347239486953864 30.151274062167438 30.61086438634158 20.89062206453712 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 6565.0 1 4358.0 2 4822.0 3 6661.5 4 4861.0 5 3776.5 6 4169.5 7 5769.0 8 7078.5 9 7206.5 10 6153.0 11 4666.0 12 3998.0 13 3746.0 14 3601.5 15 3463.0 16 3302.0 17 3173.5 18 3227.0 19 3405.0 20 3588.0 21 3884.5 22 4308.0 23 4801.0 24 5297.0 25 6080.0 26 7395.0 27 8939.5 28 10155.0 29 11203.0 30 12731.0 31 14579.5 32 16791.0 33 19163.0 34 21204.0 35 22806.0 36 24664.0 37 26443.0 38 26417.0 39 25525.0 40 25024.5 41 24858.0 42 24743.0 43 24945.5 44 25357.5 45 25394.0 46 25495.0 47 27062.0 48 31483.5 49 30865.0 50 25369.0 51 21858.5 52 19554.5 53 18174.0 54 16839.0 55 15351.0 56 13728.0 57 12194.0 58 10919.5 59 9814.5 60 8642.0 61 7310.5 62 5830.0 63 4283.0 64 2934.0 65 2118.5 66 1552.0 67 1119.5 68 824.5 69 590.0 70 451.5 71 377.0 72 325.5 73 301.5 74 237.5 75 168.0 76 116.0 77 63.0 78 42.0 79 24.5 80 15.0 81 12.0 82 9.5 83 7.5 84 5.0 85 2.0 86 1.5 87 1.5 88 0.0 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02112616884130166 2 0.019925818338954977 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.601051507040056E-4 18-19 0.0016804907032853594 20-21 1.2003505023466853E-4 22-23 0.0 24-25 0.0 26-27 5.401577260560084E-4 28-29 0.017945240010082946 30-31 0.005521612310794752 32-33 0.009782856594125484 34-35 0.011583382347645513 36-37 0.1040703885534576 38-39 0.107011247284207 40-41 0.12285587391518324 42-43 0.10575087925674297 44-45 0.1008894597222389 46-47 0.10262996795064158 48-49 0.1052707390558043 50-51 0.10359024835251893 52-53 0.07904308057952922 54-55 0.06854001368399573 56-57 0.0385312511253286 58-59 0.014824328703981563 60-61 0.009242698868069476 62-63 0.0022206484293413676 64-65 0.004921437059621409 66-67 0.41172022230491306 68-69 0.7963725407819083 70-71 0.8769160594893709 72-73 0.27391998463551354 74-75 0.1454224633593009 76-77 0.014164135927690887 78-79 0.008522488566661466 80-81 0.0018605432786373622 82-83 0.0 84-85 0.0 86-87 3.000876255866713E-4 88-89 8.402453516426797E-4 90-91 0.0037210865572747244 92-93 0.002040595853989365 94-95 0.21822372132662737 96-97 0.2249456841397688 98-99 0.013984083352338883 100-101 0.007862295790370788 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 833090.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.843868853862126 #Duplication Level Percentage of deduplicated Percentage of total 1 84.93500156712088 51.677740964524766 2 9.423376573413085 11.467093767866048 3 2.343597846109124 4.2778067998457185 4 0.9149900822116462 2.2268614625867973 5 0.5174808681435681 1.5742769037854987 6 0.33548200553438906 1.2247213888539008 7 0.23405847192498186 0.9968716078957277 8 0.17181555991700312 0.8363138715714422 9 0.13317116207182803 0.7292383848193249 >10 0.8789884793350373 10.157334506029194 >50 0.07086535287697351 2.9718200525378777 >100 0.03661935480742164 3.83250960940296 >500 0.0015835396640228661 0.5743585665200225 >1k 0.0019794245800285825 2.2729857886207556 >5k 9.897122900142912E-4 5.180066325140005 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9872 1.1849860159166477 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9843 1.181504999459842 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8968 1.0764743305045072 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7839 0.9409547587895665 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6489 0.7789074409727641 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3499 0.42000264077110516 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2349 0.28196233300123635 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2309 0.27716093099184963 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2052 0.24631192308153982 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2027 0.24331104682567312 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1661 0.19937821843978443 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1641 0.19697751743509104 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1257 0.15088405814497832 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1046 0.12555666254546327 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1032 0.12387617184217792 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 1.2003505023466853E-4 0.0 8 0.0 0.0 0.0 1.2003505023466853E-4 0.0 9 0.0 0.0 0.0 1.2003505023466853E-4 0.0 10-11 0.0 0.0 0.0 1.2003505023466853E-4 0.0 12-13 0.0 0.0 0.0 1.2003505023466853E-4 0.0 14-15 0.0 0.0 0.0 1.2003505023466853E-4 0.0 16-17 0.0 0.0 0.0 1.2003505023466853E-4 0.0 18-19 0.0 0.0 0.0 1.2003505023466853E-4 0.0 20-21 0.0 0.0 0.0 3.601051507040056E-4 0.0 22-23 1.2003505023466853E-4 0.0 0.0 0.0010202979269946825 0.0 24-25 1.2003505023466853E-4 0.0 0.0 0.0028208236805147106 0.0 26-27 1.2003505023466853E-4 0.0 0.0 0.00408119170797873 0.0 28-29 1.2003505023466853E-4 0.0 0.0 0.005101489634973412 0.0 30-31 1.2003505023466853E-4 0.0 0.0 0.009602804018773482 0.0 32-33 1.2003505023466853E-4 0.0 0.0 0.019925818338954973 0.0 34-35 1.2003505023466853E-4 0.0 0.0 0.038351198549976595 0.0 36-37 1.2003505023466853E-4 0.0 0.0 0.061878068395971625 0.0 38-39 1.2003505023466853E-4 0.0 0.0 0.09596802266261747 0.0 40-41 1.2003505023466853E-4 0.0 0.0 0.13750015004381277 0.0 42-43 1.2003505023466853E-4 0.0 0.0 0.1819731361557575 0.0 44-45 1.2003505023466853E-4 0.0 0.0 0.23412836548272095 0.0 46-47 2.4007010046933706E-4 0.0 0.0 0.300387713212258 0.0 48-49 2.4007010046933706E-4 0.0 0.0 0.3692878320469577 0.0 50-51 2.4007010046933706E-4 0.0 0.0 0.44238917763987085 0.0 52-53 2.4007010046933706E-4 0.0 0.0 0.5171109964109519 0.0 54-55 2.4007010046933706E-4 0.0 0.0 0.6019157594017454 0.0 56-57 2.4007010046933706E-4 0.0 0.0 0.6961432738359601 0.0 58-59 2.4007010046933706E-4 0.0 0.0 0.796492575832143 0.0 60-61 2.4007010046933706E-4 0.0 0.0 0.8968418778283259 0.0 62-63 2.4007010046933706E-4 0.0 0.0 0.9925098128653567 0.0 64-65 2.4007010046933706E-4 0.0 0.0 1.0940594653638862 0.0 66-67 4.801402009386741E-4 0.0 0.0 1.2034714136527866 0.0 68-69 4.801402009386741E-4 0.0 0.0 1.3401913358700739 0.0 70-71 4.801402009386741E-4 0.0 0.0 1.5120815278061195 0.0 72-73 4.801402009386741E-4 0.0 0.0 1.701076714400605 0.0 74-75 4.801402009386741E-4 0.0 0.0 1.9062166152516533 0.0 76-77 4.801402009386741E-4 0.0 0.0 2.1296018437383717 0.0 78-79 4.801402009386741E-4 0.0 0.0 2.395599515058397 0.0 80-81 4.801402009386741E-4 0.0 0.0 2.669699552269263 0.0 82-83 4.801402009386741E-4 0.0 0.0 2.9668463191251844 0.0 84-85 4.801402009386741E-4 0.0 0.0 3.2802578352879044 0.0 86-87 4.801402009386741E-4 0.0 0.0 3.604892628647565 0.0 88-89 4.801402009386741E-4 0.0 0.0 3.933848683815674 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1045 0.0 49.874355 2 GTACCTG 1220 0.0 43.499588 1 ACCTGGG 1240 0.0 41.26205 3 TATCACG 50 0.0016356532 37.904507 2 TATAACG 120 3.8853614E-9 35.535477 2 TAACGCA 125 5.7953002E-9 34.109962 4 ATAACGC 140 1.7491402E-8 30.455326 3 GTACACG 130 3.1011405E-7 29.159063 1 GTACATG 7975 0.0 28.04389 1 TACATGG 7840 0.0 27.860296 2 CCTGGGA 1125 0.0 27.37219 4 GTACCGG 70 0.008491382 27.076275 1 ACATGGG 7925 0.0 26.721264 3 CATGGGT 735 0.0 26.426842 4 CATGGGA 5380 0.0 24.47999 4 GGTATCA 9300 0.0 23.13129 1 GTATCAA 12140 0.0 22.872091 1 CATGGGG 2090 0.0 22.440765 4 ATGGGAC 765 0.0 22.294092 5 GCAACGC 55 0.0026720401 21.868774 68-69 >>END_MODULE