FastQCFastQC Report
Fri 27 May 2016
SRR939506_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939506_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences829768
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73280.8831384194136193No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69510.8377040329345069No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53990.6506638000019282No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA46540.5608796675697304No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39920.4810983310997773No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23680.28538097395898615No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA16030.1931865292467292No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12940.15594720451981758No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA12410.14955987697766124No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12250.14763162715361403No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10440.12581830101908004No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT10000.12051561400295023No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG9650.054.281522
GTACCTG10850.051.120991
ACCTGGG11500.046.8690073
GTATCGA807.1512422E-637.8179131
CGTTGCG300.004046930531.51258368-69
GTACACG1801.05501385E-1030.8145961
GTACATG66850.030.6239321
CCTGGGA11350.030.2420394
CGACGCA650.005240703529.8887355
TACATGG66900.029.7826842
ACATGGG69300.027.9857773
TATAACG1055.621185E-527.9741882
CATGGGG18000.027.014824
CGCATAG1656.806113E-826.4791458
TACGTGG1708.3779014E-825.9172632
GTATCAA113550.025.8447381
TGGGATA7000.024.962966
ATGGGGA7800.024.9072785
ACGTGGG1951.36515155E-824.6791923
GTACGTG1851.4262514E-724.5305391
Produced by FastQC (version 0.11.5)