FastQCFastQC Report
Fri 27 May 2016
SRR939506_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939506_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences829768
Sequences flagged as poor quality0
Sequence length101
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95781.1542985509202572No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95351.1491163795181303No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84781.0217313755170119No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA77640.9356832271189055No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65440.7886541780353062No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33430.4028836976118626No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA22090.26621899133251703No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21350.2573008358962987No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA20840.2511545395821483No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20270.2442851495839801No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16620.20029695047290327No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT15840.19089673258067316No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT11580.13955708101541636No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT10460.12605933224708593No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9860.11882839540690891No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCACGC801.8189894E-1259.3164563
TATCACG1000.052.2079282
TACCTGG9950.047.7002562
TCACGCA1305.456968E-1240.1647954
GTACCTG12150.039.854051
ACCTGGG11500.039.613083
TATAACG1404.6020432E-1033.9012572
ATAACGC1404.6020432E-1033.895123
TAACGCA1751.5279511E-1029.8367024
CACGCAG1852.9467628E-1028.198395
ATGGGCG1109.576424E-525.8679455
GTACATG81200.025.8413261
CGTCACT1301.0060603E-525.56251
TACATGG81700.024.921782
ACATGGG82200.023.380213
CCTGGGA11000.022.8706934
GGTATCA100850.022.8304481
CCTGGGG7800.022.5166264
ATGGGGA9850.022.1475125
GGACGCT1302.9723241E-421.888266
Produced by FastQC (version 0.11.5)