##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939506_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 829768 Sequences flagged as poor quality 0 Sequence length 101 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.931995449330415 31.0 31.0 31.0 30.0 34.0 2 31.12830815360438 31.0 31.0 34.0 28.0 34.0 3 31.118947705864773 31.0 31.0 34.0 28.0 34.0 4 35.036053451085124 35.0 35.0 37.0 32.0 37.0 5 34.66435678406494 35.0 35.0 37.0 32.0 37.0 6 34.76366647062793 35.0 35.0 37.0 32.0 37.0 7 34.622251038844595 35.0 35.0 37.0 30.0 37.0 8 34.54088130658208 35.0 35.0 37.0 30.0 37.0 9 36.01175629814599 37.0 35.0 39.0 30.0 39.0 10-11 35.815858770162265 37.0 35.0 39.0 30.0 39.0 12-13 35.79891005678696 37.0 35.0 39.0 30.0 39.0 14-15 36.528805039480915 38.0 35.0 40.0 30.0 41.0 16-17 36.487978567503205 38.0 35.0 40.0 30.5 41.0 18-19 36.5882951620212 38.0 35.0 40.0 30.0 41.0 20-21 36.488349755594335 38.0 35.0 40.0 30.0 41.0 22-23 36.257791937023356 38.0 34.0 40.0 30.0 41.0 24-25 36.07226116215617 38.0 34.0 40.0 29.5 41.0 26-27 35.550069416993665 38.0 34.0 40.0 27.5 41.0 28-29 35.321541683940566 38.0 34.0 40.0 27.0 41.0 30-31 34.94591078470127 37.0 33.0 40.0 27.0 41.0 32-33 34.51154961386797 37.0 33.0 40.0 25.5 41.0 34-35 34.5228087851062 37.0 33.0 40.0 25.0 41.0 36-37 34.252905631453615 37.0 33.0 40.0 24.0 41.0 38-39 34.03035728058927 37.0 33.0 40.0 24.0 41.0 40-41 33.852435259012154 37.0 33.0 40.0 23.5 41.0 42-43 33.64664641200914 37.0 33.0 40.0 23.0 41.0 44-45 33.18182672747081 36.5 32.0 40.0 20.5 41.0 46-47 32.64815767780874 36.0 31.0 40.0 18.0 41.0 48-49 32.166238635377596 36.0 31.0 39.0 14.0 41.0 50-51 31.9125189209514 36.0 30.0 39.0 12.0 40.0 52-53 31.80312629554285 35.5 30.0 39.0 9.5 40.0 54-55 31.50046880573847 35.0 30.0 39.0 8.5 40.0 56-57 31.05392049343913 35.0 29.5 39.0 7.5 40.0 58-59 30.649977463580182 35.0 28.0 38.5 6.5 40.0 60-61 30.326157431956887 35.0 28.0 38.0 2.0 40.0 62-63 30.18095298926929 34.5 28.0 38.0 2.0 40.0 64-65 29.76601471736678 34.0 28.0 38.0 2.0 40.0 66-67 28.979512948197566 33.5 27.0 37.5 2.0 39.5 68-69 25.74836339796184 31.0 15.5 36.0 2.0 39.0 70-71 24.538751193104577 29.5 11.5 35.0 2.0 38.0 72-73 25.492126714937186 31.0 16.5 35.0 2.0 38.0 74-75 26.18262514341358 31.0 21.0 35.0 2.0 37.5 76-77 25.797110758669895 30.5 21.0 34.5 2.0 36.0 78-79 26.856821424783796 32.0 24.5 35.0 2.0 37.0 80-81 26.858377883938644 32.0 25.0 35.0 2.0 36.5 82-83 26.54367546109274 32.0 24.5 35.0 2.0 36.0 84-85 26.186167699887196 32.0 23.5 35.0 2.0 36.0 86-87 25.738881229452087 32.0 22.5 34.5 2.0 35.0 88-89 25.265659798883544 31.5 20.0 34.0 2.0 35.0 90-91 24.67400044349746 31.0 18.5 34.0 2.0 35.0 92-93 24.163517392813414 31.0 14.5 34.0 2.0 35.0 94-95 23.38246172424099 30.5 4.5 34.0 2.0 35.0 96-97 22.745004627799577 30.0 2.0 34.0 2.0 35.0 98-99 21.962629915831897 30.0 2.0 34.0 2.0 35.0 100-101 20.69568602308115 28.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.20467080517750347 1101 2 0.11255477143249593 1101 3 0.2379015196980525 1101 4 0.30356376519549144 1101 5 0.47915365008640975 1101 6 0.20845173303950304 1101 7 0.4242219343997746 1101 8 0.4451441296674403 1101 9 0.5728514095288659 1101 10-11 0.6010259210114128 1101 12-13 0.5230361353339887 1101 14-15 0.583451662025368 1101 16-17 0.6675275942034133 1101 18-19 0.39870606975902945 1101 20-21 0.5823164778303109 1101 22-23 0.5901690622887088 1101 24-25 0.6943730627069797 1101 26-27 0.732669004986839 1101 28-29 0.7554059932173871 1101 30-31 0.5909570553803221 1101 32-33 0.5969808674588108 1101 34-35 0.574111358654207 1101 36-37 0.6680359759040826 1101 38-39 0.91648950579836 1101 40-41 1.0352204790740487 1101 42-43 0.5713395383213538 1101 44-45 0.7032234584936248 1101 46-47 0.6067679398081793 1101 48-49 0.6339635229701983 1101 50-51 0.2453424620227267 1101 52-53 0.164693228502113 1101 54-55 0.34376002975908904 1101 56-57 0.2705355181057314 1101 58-59 0.18599647842421874 1101 60-61 -0.2831776482302608 1101 62-63 -0.19579896025940258 1101 64-65 -0.38461561294046476 1101 66-67 -0.4966453573385472 1101 68-69 -6.86994519477134 1101 70-71 -7.949176821043242 1101 72-73 -4.7085325652263315 1101 74-75 -2.834106673166648 1101 76-77 -0.4120113977633686 1101 78-79 -0.27377050666527225 1101 80-81 -0.6236482697558365 1101 82-83 -0.685215933730781 1101 84-85 -0.8851458247338222 1101 86-87 -0.6582727955689656 1101 88-89 -0.6498096704820782 1101 90-91 -0.5414877962934135 1101 92-93 -0.7383842375989218 1101 94-95 -0.7077114133355273 1101 96-97 -1.0394660332121788 1101 98-99 -0.7466796163498906 1101 100-101 -0.31645223796672184 1102 1 0.40381331991388336 1102 2 0.43443079730998946 1102 3 0.5204682272789647 1102 4 0.5268988607858489 1102 5 0.5560491420923057 1102 6 0.39425207239482774 1102 7 0.5990954012691247 1102 8 0.6363222094752672 1102 9 0.6759816789959885 1102 10-11 0.7481253821826499 1102 12-13 0.6401249306752916 1102 14-15 0.8658746643576194 1102 16-17 0.6910214247657009 1102 18-19 0.6023258599493673 1102 20-21 0.7685624352961895 1102 22-23 0.8241047271627977 1102 24-25 0.6740592051702947 1102 26-27 0.6719014607513358 1102 28-29 0.28906136693672835 1102 30-31 0.725943086966474 1102 32-33 0.7629900935994556 1102 34-35 0.7086478169394752 1102 36-37 1.2090506404314283 1102 38-39 1.2923354221302574 1102 40-41 0.9056985429292794 1102 42-43 0.798519078912804 1102 44-45 1.0611081963370168 1102 46-47 1.3323495835061792 1102 48-49 1.4781448447157004 1102 50-51 1.1361535001679073 1102 52-53 0.7598524710567993 1102 54-55 0.6144170000569318 1102 56-57 0.9515889698662541 1102 58-59 0.8077293264117529 1102 60-61 1.0350655353288047 1102 62-63 0.9013246934926897 1102 64-65 0.7690198002516766 1102 66-67 0.5311991825871196 1102 68-69 -6.1450484581988825 1102 70-71 -7.177127623749119 1102 72-73 -1.2337093292741805 1102 74-75 0.633443626966379 1102 76-77 1.1290975321335175 1102 78-79 0.9898205161073932 1102 80-81 0.7571686616229307 1102 82-83 0.7651352212507163 1102 84-85 0.5959002440047456 1102 86-87 0.6573975366802074 1102 88-89 0.520770437283673 1102 90-91 0.5394189179058522 1102 92-93 0.4156776769505086 1102 94-95 0.31859126155969264 1102 96-97 -0.23634881587314993 1102 98-99 -0.1795817899906531 1102 100-101 -0.3107290775587046 1103 1 0.4056939217582425 1103 2 0.3195768901752203 1103 3 0.27828735039820174 1103 4 0.31476749045742025 1103 5 0.3396479125178047 1103 6 0.35440350420198286 1103 7 0.3369177601107367 1103 8 0.414904315930535 1103 9 0.506995430816076 1103 10-11 0.5891316918412741 1103 12-13 0.42618996869503434 1103 14-15 0.6073598609277511 1103 16-17 0.6410476341169371 1103 18-19 0.7438841838451751 1103 20-21 0.5899013772149715 1103 22-23 0.48755206578114496 1103 24-25 0.7155638160746847 1103 26-27 1.0260726304649737 1103 28-29 1.0856670941653306 1103 30-31 0.9375800389159892 1103 32-33 0.9589512649309526 1103 34-35 0.9825271244948866 1103 36-37 1.2491528892584753 1103 38-39 1.2242014632239275 1103 40-41 1.169987816509611 1103 42-43 1.0668439454392598 1103 44-45 1.0883756287913116 1103 46-47 0.9051973427684317 1103 48-49 1.1759917950473664 1103 50-51 0.5450271452091613 1103 52-53 0.511379408445567 1103 54-55 0.5113715381575616 1103 56-57 0.7056747165091899 1103 58-59 0.9798937011084057 1103 60-61 0.6676861468803317 1103 62-63 0.3415421340362883 1103 64-65 0.5953158688489886 1103 66-67 0.4306588169504941 1103 68-69 -6.52302419028107 1103 70-71 -7.560057246787462 1103 72-73 -0.779756247248617 1103 74-75 0.7099671668466563 1103 76-77 1.029549886121547 1103 78-79 0.7879444442743591 1103 80-81 0.6883603781736483 1103 82-83 0.6050012623443912 1103 84-85 0.1813407876061035 1103 86-87 0.4182886546222875 1103 88-89 0.6645691753519465 1103 90-91 0.13332411124026677 1103 92-93 0.16907227329414454 1103 94-95 0.12046021480350433 1103 96-97 0.3705549213861943 1103 98-99 0.3618898165455029 1103 100-101 0.4176838006163486 1104 1 0.20515712522144725 1104 2 0.28824355684188774 1104 3 0.21300596511681746 1104 4 0.20279346448339908 1104 5 0.42027994714983663 1104 6 0.24078445658293646 1104 7 0.40518615837913075 1104 8 0.4053112423374614 1104 9 0.4689175087381585 1104 10-11 0.4635472762568611 1104 12-13 0.19862286912793792 1104 14-15 0.5451520687199576 1104 16-17 0.6244372445065451 1104 18-19 0.49736037432136726 1104 20-21 0.7192476975613005 1104 22-23 0.6231884294175103 1104 24-25 0.6515378420487039 1104 26-27 0.9693323707247146 1104 28-29 0.7303857088839436 1104 30-31 0.7058570951930463 1104 32-33 0.45987767185736317 1104 34-35 0.4451981201658768 1104 36-37 0.8953779974835854 1104 38-39 0.6530369610594349 1104 40-41 0.8386155221373102 1104 42-43 0.5730517376470488 1104 44-45 0.6125531179688011 1104 46-47 0.8262060007770913 1104 48-49 0.5661779422335158 1104 50-51 0.5097544179364348 1104 52-53 0.3107820058481714 1104 54-55 0.43290400569002685 1104 56-57 0.3779517727862469 1104 58-59 0.5938633980780992 1104 60-61 0.8933225105166969 1104 62-63 0.5133646448588003 1104 64-65 0.37340677793990196 1104 66-67 0.3854856567773304 1104 68-69 -6.451409471666349 1104 70-71 -4.189221748951962 1104 72-73 0.513170159677788 1104 74-75 1.227967166846657 1104 76-77 1.3006710982427592 1104 78-79 0.921476911806824 1104 80-81 0.696494577307849 1104 82-83 0.7383908727340014 1104 84-85 0.5985139477792636 1104 86-87 0.6608514251850579 1104 88-89 0.48894146972424224 1104 90-91 0.6486790895952446 1104 92-93 0.7634142646361362 1104 94-95 0.7267286130719057 1104 96-97 0.6914553542866244 1104 98-99 0.44517553083121797 1104 100-101 0.4076405105730565 1105 1 0.17698989650173402 1105 2 0.17986418299290108 1105 3 0.05858727147160181 1105 4 0.09036970040217085 1105 5 0.26595572625103614 1105 6 0.1256173952832782 1105 7 0.09369755490158127 1105 8 0.20045127725650502 1105 9 0.24158738030305926 1105 10-11 0.17127849531404848 1105 12-13 0.22510393870292944 1105 14-15 0.23858322320083403 1105 16-17 0.3219600255928654 1105 18-19 0.3263593219667271 1105 20-21 0.37066538668617 1105 22-23 0.2523697453391591 1105 24-25 0.21108078529330498 1105 26-27 0.2516259059188002 1105 28-29 0.38800332147393135 1105 30-31 0.3146155558852115 1105 32-33 0.4100546587746763 1105 34-35 0.4395626414641072 1105 36-37 0.2405467329838089 1105 38-39 0.35747060292401045 1105 40-41 0.27771946607551 1105 42-43 0.34489287993018536 1105 44-45 0.38239299264292725 1105 46-47 0.3905927650257368 1105 48-49 0.5591457019139767 1105 50-51 0.4263720544435685 1105 52-53 0.3728506002372036 1105 54-55 0.34765251684737564 1105 56-57 0.4541924749937998 1105 58-59 0.570551948937922 1105 60-61 0.8957082463278461 1105 62-63 0.2913542887324212 1105 64-65 0.39274917587345826 1105 66-67 0.6371939392867212 1105 68-69 -2.562585358394724 1105 70-71 -2.6719625348695466 1105 72-73 0.6431419374396228 1105 74-75 1.0089853199642569 1105 76-77 1.191845071599051 1105 78-79 0.5015766463264484 1105 80-81 0.2818315699652807 1105 82-83 0.6523698495582835 1105 84-85 0.5039485224127347 1105 86-87 0.32809449519055534 1105 88-89 0.3015541792982752 1105 90-91 0.5122538665678107 1105 92-93 0.5024392819760699 1105 94-95 0.5434562684735909 1105 96-97 0.15358254569558483 1105 98-99 0.09026235008930783 1105 100-101 0.25861513447585693 1106 1 0.15398809683146197 1106 2 0.2986839516786368 1106 3 0.15962805980269223 1106 4 0.1974463059471745 1106 5 0.4513003694003288 1106 6 0.31647053967220273 1106 7 0.25171112164669296 1106 8 0.40715032959796105 1106 9 0.3880666966800561 1106 10-11 0.4608108327459348 1106 12-13 0.286556385876942 1106 14-15 0.58255999109371 1106 16-17 0.4054810867690648 1106 18-19 0.2570550266697822 1106 20-21 0.3050598542732246 1106 22-23 0.24303725940301035 1106 24-25 0.12466892508867744 1106 26-27 0.5834901377868036 1106 28-29 0.3947917280735638 1106 30-31 0.17780978183822072 1106 32-33 0.717692560082277 1106 34-35 0.6409117649114151 1106 36-37 0.6429127330593261 1106 38-39 0.862938202566589 1106 40-41 0.4445319102290952 1106 42-43 0.3464150485957873 1106 44-45 0.5227817465915052 1106 46-47 0.7979972126024677 1106 48-49 0.8755082285000242 1106 50-51 0.7692432207054125 1106 52-53 0.6203920996268302 1106 54-55 0.3509881994006463 1106 56-57 0.3605617975375033 1106 58-59 0.6015389988630417 1106 60-61 0.6817362607755477 1106 62-63 0.4709362114850393 1106 64-65 0.12893513341130713 1106 66-67 0.44693607044870376 1106 68-69 -2.5566577730991646 1106 70-71 -3.1321841586000225 1106 72-73 0.5130937364806378 1106 74-75 1.0797406780734704 1106 76-77 1.1079527497727035 1106 78-79 0.5943609753514814 1106 80-81 0.1916737527262029 1106 82-83 0.22867649827019676 1106 84-85 0.15331800856282385 1106 86-87 0.091964541377898 1106 88-89 0.36354086425529886 1106 90-91 0.4420862329454387 1106 92-93 0.6035480298837967 1106 94-95 0.7035822453925071 1106 96-97 0.5176665386982648 1106 98-99 0.6540512223378911 1106 100-101 0.5572672695392242 1107 1 0.06070714329968396 1107 2 0.008145738933684754 1107 3 0.0013831259544723196 1107 4 -0.12732731766899263 1107 5 0.26528351393670135 1107 6 0.1228923787585785 1107 7 -0.12392712863483268 1107 8 0.057911087939565675 1107 9 0.23249817186735555 1107 10-11 -0.017837027926539406 1107 12-13 0.22111805640867743 1107 14-15 0.07123602990549927 1107 16-17 -0.026221633861133853 1107 18-19 0.10739208452238103 1107 20-21 0.26446893606696165 1107 22-23 0.05131278812877582 1107 24-25 0.22351608953484714 1107 26-27 0.5059591186945767 1107 28-29 0.6686338790734183 1107 30-31 0.23221467290503028 1107 32-33 0.3185913810225358 1107 34-35 0.01607823703923117 1107 36-37 0.10842022237682869 1107 38-39 0.17281674861573748 1107 40-41 0.009922676232271499 1107 42-43 -0.2886497662666443 1107 44-45 -0.31982817643281436 1107 46-47 -0.200836839753336 1107 48-49 -0.24954254871271075 1107 50-51 -0.07424341267539702 1107 52-53 -0.017271994327728635 1107 54-55 -0.17692127061380347 1107 56-57 0.27072341047887605 1107 58-59 0.11001043764500551 1107 60-61 0.11885093243338218 1107 62-63 -0.03893642126845265 1107 64-65 -0.243963718380936 1107 66-67 -0.17084392410078308 1107 68-69 -2.55324734410242 1107 70-71 -2.789967598286978 1107 72-73 0.4688687124418074 1107 74-75 0.861875583486448 1107 76-77 0.7645818113069716 1107 78-79 0.4310486042227595 1107 80-81 0.35615592799628715 1107 82-83 0.38150819559173144 1107 84-85 0.2405416905406419 1107 86-87 0.11368433343557172 1107 88-89 0.046310549102347665 1107 90-91 -0.2524830477407072 1107 92-93 -0.26860009045948274 1107 94-95 0.06902777365548829 1107 96-97 -0.007810767101393168 1107 98-99 -0.19465839370925053 1107 100-101 -0.16429620583318538 1108 1 -0.11480461387468921 1108 2 -0.1568969139250065 1108 3 0.021814847957589478 1108 4 -0.026927270362627098 1108 5 -0.1648727513286019 1108 6 -0.1937800298038752 1108 7 0.05353344510911029 1108 8 -0.00628930593734367 1108 9 -0.004871799903085616 1108 10-11 0.15728008103750568 1108 12-13 1.0991662865222906E-4 1108 14-15 0.15545292959029666 1108 16-17 0.10365876330665458 1108 18-19 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94-95 1.3077946785593326 2316 96-97 1.417308630585005 2316 98-99 1.0789716308736104 2316 100-101 0.9612488705612385 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 12.0 4 53.0 5 131.0 6 390.0 7 770.0 8 1402.0 9 2213.0 10 3562.0 11 4986.0 12 6613.0 13 8089.0 14 8675.0 15 10794.0 16 12763.0 17 14071.0 18 12173.0 19 9587.0 20 9055.0 21 9150.0 22 9784.0 23 10991.0 24 12931.0 25 15876.0 26 19599.0 27 24344.0 28 29708.0 29 35081.0 30 41806.0 31 48428.0 32 56038.0 33 65748.0 34 76304.0 35 85090.0 36 82142.0 37 68329.0 38 31032.0 39 2046.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.68999809542637 20.594468057407227 14.815774691350583 18.899759155815822 2 15.611835198227636 23.78574949947386 40.268337457676765 20.33407784462174 3 18.946471526843776 31.434480165444633 30.81032679171427 18.80872151599732 4 11.759603843346826 19.249425488410246 37.781450362801806 31.209520305441124 5 11.117444876158157 40.32633217959719 34.154727586506105 14.401495357738547 6 25.491342158290024 37.79899923834132 20.962967962129174 15.74669064123948 7 24.654722765881548 36.0212734161838 23.098143095419445 16.225860722515208 8 25.55184099651951 33.64663375786967 21.958185902565535 18.843339343045283 9 26.84184012880709 17.788225142449456 22.93797784440952 32.431956884333935 10-11 23.242686208278467 28.63890705179391 28.39574174458302 19.722664995344605 12-13 25.541838200557265 27.03213428331775 29.164537557485946 18.26148995863904 14-15 22.19807223223841 27.377110228401193 30.19934487712228 20.22547266223812 16-17 18.666007845594837 30.627888619066844 30.754671503377462 19.951432031960856 18-19 18.270720412645712 28.432121045955643 34.96401100308219 18.333147538316457 20-21 20.081878308153605 27.224537461073457 34.64215298734104 18.0514312434319 22-23 19.459100517495294 27.597479289341813 34.358301014984946 18.585119178177944 24-25 18.985987838410303 27.839326249618107 35.02805385037665 18.14663206159494 26-27 19.04482702517038 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18.891218472978736 52-53 19.19777165492002 29.92216467807769 31.89306837570766 18.986995291294622 54-55 18.784502075927023 29.684942581190843 32.039810052828074 19.490745290054058 56-57 19.296393001540586 30.075980587158885 31.35244962955998 19.275176781740548 58-59 19.368191642347337 29.568447914444175 31.57185442876998 19.49150601443851 60-61 19.294087301659047 29.659976604972982 31.96217502638118 19.083761066986796 62-63 19.584177961178856 29.4619851184809 31.982732231480654 18.97110468885959 64-65 19.257003705460203 29.715573237526083 31.813867665234657 19.21355539177905 66-67 18.952745926372963 29.836089318044657 31.694508147254076 19.516656608328304 68-69 19.388554591606052 29.78737479024236 31.466103469307864 19.357967148843716 70-71 19.177244505825154 29.854428833157254 31.498335061692075 19.469991599325514 72-73 19.40382989582867 29.760066085235927 30.99589869994473 19.840205318990677 74-75 18.951541674931256 30.386216052132927 31.009421330055233 19.652820942880584 76-77 18.511275707841072 30.12617249007429 31.60603367497754 19.7565181271071 78-79 18.856628705355984 30.1487174573214 31.258448485856565 19.73620535146605 80-81 18.763509445991442 30.551971784051336 30.953963483895926 19.730555286061293 82-83 18.83120991665617 30.60184596546814 30.61088477268304 19.95605934519265 84-85 18.68398725420722 30.350858798405326 30.602617129689886 20.362536817697563 86-87 18.774005695684608 30.30276552845372 30.735485705952748 20.187743069908926 88-89 18.957705520415104 30.014684871810164 30.634136994126653 20.393472613648072 90-91 18.673699421442834 30.20476982752958 30.861966419459673 20.259564331567915 92-93 18.592243218797247 30.595302013827247 30.66255211732371 20.149902650051793 94-95 18.365947685577986 30.40591104606532 30.55696762806191 20.67117364029478 96-97 18.38282143354445 30.239410326163945 30.387817361694225 20.98995087859738 98-99 18.37431554029378 30.284788270985175 30.123579403352423 21.217316785368624 100-101 18.32377529324983 30.135246869832272 30.62092306802239 20.92005476889551 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 6497.0 1 4432.0 2 4948.0 3 6530.5 4 4617.5 5 3632.0 6 4102.0 7 5813.0 8 7103.0 9 7089.5 10 6006.5 11 4614.0 12 3891.5 13 3546.5 14 3465.0 15 3368.0 16 3211.0 17 3164.5 18 3298.5 19 3435.0 20 3543.0 21 3843.0 22 4323.5 23 4816.0 24 5326.0 25 6030.5 26 7382.0 27 8912.5 28 9985.5 29 11238.0 30 12938.0 31 14827.0 32 16804.0 33 18943.5 34 21150.0 35 22860.5 36 24656.5 37 26304.5 38 26342.5 39 25412.5 40 24861.5 41 24813.0 42 24836.5 43 24932.0 44 25204.0 45 25389.5 46 25387.0 47 26925.0 48 31616.5 49 30844.5 50 25245.5 51 22046.0 52 19445.5 53 17919.0 54 16653.0 55 15223.5 56 13680.5 57 11995.5 58 10644.5 59 9453.0 60 8482.0 61 7364.0 62 5915.0 63 4320.0 64 2983.0 65 2165.5 66 1556.5 67 1159.5 68 848.5 69 567.5 70 421.0 71 353.5 72 312.0 73 288.0 74 237.5 75 186.5 76 130.5 77 76.5 78 50.0 79 29.5 80 13.5 81 8.0 82 13.0 83 9.0 84 3.0 85 1.5 86 0.5 87 0.0 88 1.0 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02241590420454874 2 0.01699270157441598 3 4.8206245601180086E-4 4 0.09677403804436903 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.010304084997252244 12-13 0.0 14-15 0.0 16-17 3.615468420088507E-4 18-19 6.025780700147511E-5 20-21 0.0 22-23 2.4103122800590043E-4 24-25 0.0029526325430722805 26-27 0.002771859122067855 28-29 0.001084640526026552 30-31 1.8077342100442534E-4 32-33 6.628358770162262E-4 34-35 0.0033141793850811315 36-37 0.08098649260998254 38-39 0.09309831181727905 40-41 0.07435813383982029 42-43 0.07797360225990879 44-45 0.07622612585686601 46-47 0.07502096971683651 48-49 0.06977854050770818 50-51 0.06971828270070671 52-53 0.05459357314333645 54-55 0.04573567551411961 56-57 0.025790341396631347 58-59 0.02181332613453399 60-61 0.012593881663308298 62-63 0.001687218596041303 64-65 0.005603976051137185 66-67 0.1528137985557409 68-69 0.7111023804244078 70-71 0.8695201550312859 72-73 0.35636467060672383 74-75 0.051399909372258266 76-77 0.19475323222876756 78-79 0.012955428505317149 80-81 0.0029526325430722805 82-83 0.0015064451750368777 84-85 4.8206245601180086E-4 86-87 0.0020487654380501536 88-89 3.012890350073756E-4 90-91 0.0037962418410929315 92-93 0.0035552106130870313 94-95 0.07333375112079521 96-97 0.03525081709586294 98-99 0.011931045786292073 100-101 0.012051561400295022 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 829768.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.50835707650395 #Duplication Level Percentage of deduplicated Percentage of total 1 85.22349772888145 52.41957329616663 2 9.306500050055883 11.448550564226782 3 2.2226328864466294 4.1013149168861975 4 0.9079510360041794 2.23386306122107 5 0.5107749635476485 1.5708464421813526 6 0.330265150594062 1.2188440087598948 7 0.21730171910500024 0.9356110212433958 8 0.1912482382952232 0.9410691945051924 9 0.13142254619890525 0.7275226409555041 >10 0.8495509453366551 9.928999583401465 >50 0.07059848595285202 3.00375779648935 >100 0.033917912007175074 3.6693478183597867 >500 0.0015775772997691894 0.6265982681704618 >1k 0.0017747744622403385 2.092057090476989 >5k 9.859858123557434E-4 5.0820442969559005 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9578 1.1542985509202572 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9535 1.1491163795181303 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8478 1.0217313755170119 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7764 0.9356832271189055 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6544 0.7886541780353062 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3343 0.4028836976118626 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 2209 0.26621899133251703 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2135 0.2573008358962987 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 2084 0.2511545395821483 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2027 0.2442851495839801 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1662 0.20029695047290327 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1584 0.19089673258067316 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1158 0.13955708101541636 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1046 0.12605933224708593 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 986 0.11882839540690891 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.2051561400295022E-4 0.0 14-15 0.0 0.0 0.0 1.2051561400295022E-4 0.0 16-17 0.0 0.0 0.0 2.4103122800590043E-4 0.0 18-19 0.0 0.0 0.0 3.615468420088507E-4 0.0 20-21 0.0 0.0 0.0 3.615468420088507E-4 0.0 22-23 1.2051561400295022E-4 0.0 0.0 0.0011448983330280271 0.0 24-25 1.2051561400295022E-4 0.0 0.0 0.0023500544730575296 0.0 26-27 1.2051561400295022E-4 0.0 0.0 0.003977015262097357 0.0 28-29 1.2051561400295022E-4 0.0 0.0 0.006206554121151936 0.0 30-31 1.2051561400295022E-4 0.0 0.0 0.011629756751284696 0.0 32-33 1.2051561400295022E-4 0.0 0.0 0.022114615169541364 0.0 34-35 1.8077342100442532E-4 0.0 0.0 0.03832396525293817 0.0 36-37 3.615468420088507E-4 0.0 0.0 0.061402705334503135 0.0 38-39 4.8206245601180086E-4 0.0 0.0 0.09508681944832773 0.0 40-41 4.8206245601180086E-4 0.0 0.0 0.13515826110430867 0.0 42-43 4.8206245601180086E-4 0.0 0.0 0.17962852267139728 0.0 44-45 6.025780700147511E-4 0.0 0.0 0.23277590844669838 0.0 46-47 8.436092980206516E-4 0.0 0.0 0.2967094416752635 0.0 48-49 8.436092980206516E-4 0.0 0.0 0.3649212792009333 0.0 50-51 8.436092980206516E-4 0.0 0.0 0.4357242024276665 0.0 52-53 8.436092980206516E-4 0.0 0.0 0.5135170312665709 0.0 54-55 9.641249120236017E-4 0.0 0.0 0.597757445454633 0.0 56-57 9.641249120236017E-4 0.0 0.0 0.692482718060952 0.0 58-59 9.641249120236017E-4 0.0 0.0 0.7963069195244936 0.0 60-61 9.641249120236017E-4 0.0 0.0 0.8997093163390248 0.0 62-63 9.641249120236017E-4 0.0 0.0 1.0059438300826256 0.0 64-65 9.641249120236017E-4 0.0 0.0 1.1108526720721936 0.0 66-67 9.641249120236017E-4 0.0 0.0 1.2271502395850407 0.0 68-69 9.641249120236017E-4 0.0 0.0 1.3665868049864542 0.0 70-71 9.641249120236017E-4 0.0 0.0 1.543262695114779 0.0 72-73 9.641249120236017E-4 0.0 0.0 1.7324722090994111 0.0 74-75 9.641249120236017E-4 0.0 0.0 1.9377705575534367 0.0 76-77 9.641249120236017E-4 0.0 0.0 2.1631347557389535 0.0 78-79 0.0010243827190250768 0.0 0.0 2.419772755758236 0.0 80-81 0.001084640526026552 0.0 0.0 2.703707542349187 0.0 82-83 0.001084640526026552 0.0 0.0 2.992824500342264 0.0 84-85 0.0011448983330280271 0.0 0.0 3.3098408229770246 0.0 86-87 0.0012051561400295023 0.0 0.0 3.635835558855005 0.0 88-89 0.0012051561400295023 0.0 0.0 3.987379604901611 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCACGC 80 1.8189894E-12 59.316456 3 TATCACG 100 0.0 52.207928 2 TACCTGG 995 0.0 47.700256 2 TCACGCA 130 5.456968E-12 40.164795 4 GTACCTG 1215 0.0 39.85405 1 ACCTGGG 1150 0.0 39.61308 3 TATAACG 140 4.6020432E-10 33.901257 2 ATAACGC 140 4.6020432E-10 33.89512 3 TAACGCA 175 1.5279511E-10 29.836702 4 CACGCAG 185 2.9467628E-10 28.19839 5 ATGGGCG 110 9.576424E-5 25.867945 5 GTACATG 8120 0.0 25.841326 1 CGTCACT 130 1.0060603E-5 25.5625 1 TACATGG 8170 0.0 24.92178 2 ACATGGG 8220 0.0 23.38021 3 CCTGGGA 1100 0.0 22.870693 4 GGTATCA 10085 0.0 22.830448 1 CCTGGGG 780 0.0 22.516626 4 ATGGGGA 985 0.0 22.147512 5 GGACGCT 130 2.9723241E-4 21.88826 6 >>END_MODULE