FastQCFastQC Report
Fri 27 May 2016
SRR939505_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939505_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2032417
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54750.269383694389488No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45200.22239530568775992No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28720.1413095836139926No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35900.055.633871
GTATCAA69250.045.239751
TATCAAC89050.035.207582
ATCAACG90300.034.504853
TCAACGC92600.033.570934
CAACGCA92650.033.3584565
AACGCAG96400.032.150986
GTGGTAT18550.029.2665771
TGGTATC18000.028.6693342
ACGCAGA111450.027.388727
CGCAGAG112600.026.8994488
GTACATG84950.025.0353581
GCAGAGT116300.024.5195039
TACATGG85550.024.4130232
GAGTACT61250.023.32985512-13
ACATGGG87850.022.6096153
GTACTTT68050.021.17170314-15
CAGAGTA110750.021.15669810-11
AGAGTAC106150.020.48367310-11
CATGGGG46050.020.459624
Produced by FastQC (version 0.11.5)