FastQCFastQC Report
Fri 27 May 2016
SRR939505_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939505_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2032417
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76100.37443103457607374No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71530.35194549150100596No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46130.22697113830478688No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20410.10042230506830045No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41450.059.351251
GTATCAA75450.042.217941
ATCAACG98050.032.1778563
TATCAAC99350.031.8600142
TCAACGC99200.031.8048294
GTGGTAT18850.031.4329851
TGGTATC18150.031.06762
CAACGCA101500.030.9441095
AACGCAG104850.029.9102526
ACGCAGA121800.025.6700787
CGCAGAG123200.025.1861118
GTACATG93300.024.0307041
TACATGG95100.023.1193052
GCAGAGT131900.022.9861229
ACATGGG93700.022.3972473
GAGTACT75150.021.7170812-13
CAGAGTA128300.020.73259410-11
GTACTTT78050.020.69772714-15
TAGCGCG1708.1231265E-519.5065124
TAGGACG1708.1231265E-519.5065124
Produced by FastQC (version 0.11.5)