FastQCFastQC Report
Fri 27 May 2016
SRR939504_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939504_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2018240
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56280.2788568257491676No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42820.2121650547011257No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27960.13853654669414936No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34250.059.6557581
GTATCAA66200.045.9180561
TATCAAC88550.034.0019232
ATCAACG90800.033.5716823
TCAACGC93400.032.6114164
CAACGCA93850.031.7008275
AACGCAG95500.031.0786696
ACGCAGA109150.026.6153857
GTACATG85950.026.3357321
CGCAGAG110700.026.026028
TGGTATC16350.025.96622
GTGGTAT17250.025.837691
TACATGG89250.025.2604262
ACATGGG93450.023.8730753
GCAGAGT114700.023.8097089
GAGTACT63500.022.42447512-13
GTACTTT70600.020.67164414-15
AGAGTAC105250.020.4267510-11
GTATAGG5900.020.3709831
AGTACTT68200.020.35095212-13
Produced by FastQC (version 0.11.5)