FastQCFastQC Report
Fri 27 May 2016
SRR939504_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939504_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2018240
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76250.37780442365625494No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68970.3417333914697955No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44210.21905224353892502No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42600.058.136431
GTATCAA75250.039.784171
GTGGTAT19900.029.3250981
ATCAACG102000.029.06143
TATCAAC103150.028.8782022
TCAACGC103200.028.6803634
TGGTATC19000.028.4597432
CAACGCA104950.028.1825645
AACGCAG108700.027.2103046
ACGCAGA125650.023.3887797
CGCAGAG126950.022.9625848
GTACATG93750.021.5588591
GCAGAGT134200.021.0156489
GAGTACT73750.020.59738512-13
TACATGG96350.020.4795952
ACATGGG93900.019.9006523
CAGAGTA131650.019.44197810-11
GTACTTT77250.019.29604114-15
AGAGTAC125850.019.28343210-11
AGTACTT77750.019.17195112-13
Produced by FastQC (version 0.11.5)