##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939504_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2018240 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.896445417789757 31.0 31.0 31.0 28.0 34.0 2 31.152383760107817 31.0 31.0 34.0 28.0 34.0 3 31.16289588948787 31.0 31.0 34.0 28.0 34.0 4 35.07690512525765 35.0 35.0 37.0 32.0 37.0 5 34.6492448866339 35.0 35.0 37.0 32.0 37.0 6 34.70510246551451 35.0 35.0 37.0 31.0 37.0 7 34.54198608688758 35.0 35.0 37.0 30.0 37.0 8 34.44849026874901 35.0 35.0 37.0 30.0 37.0 9 35.95376268431901 37.0 35.0 39.0 30.0 39.0 10-11 35.751825600523226 37.0 35.0 39.0 30.0 39.0 12-13 35.73486156254955 37.0 35.0 39.0 30.0 39.0 14-15 36.58679988504836 38.0 35.5 40.0 30.5 41.0 16-17 36.491063500872045 38.0 35.0 40.0 30.0 41.0 18-19 36.473596053987634 38.0 35.0 40.0 30.0 41.0 20-21 36.33597639527509 38.0 34.5 40.0 29.5 41.0 22-23 36.1800326026637 38.0 34.0 40.0 29.0 41.0 24-25 36.01689789123196 38.0 34.0 40.0 29.0 41.0 26-27 35.56186380212462 38.0 34.0 40.0 27.0 41.0 28-29 35.344606934755035 38.0 34.0 40.0 27.0 41.0 30-31 35.053964345172034 37.5 33.0 40.0 27.0 41.0 32-33 34.84547650428096 37.0 33.0 40.0 26.0 41.0 34-35 35.11767753091803 38.0 33.0 40.0 27.0 41.0 36-37 34.99259429007452 38.0 33.0 40.0 26.0 41.0 38-39 34.81853322697003 38.0 33.0 40.0 25.0 41.0 40-41 34.6682827612177 37.5 33.0 40.0 25.0 41.0 42-43 34.50002502180118 37.0 33.0 40.0 25.0 41.0 44-45 34.12687143253528 37.0 33.0 40.0 24.0 41.0 46-47 33.74062153163152 37.0 32.0 40.0 23.0 41.0 48-49 33.37695665530363 37.0 31.5 40.0 22.5 41.0 50-51 33.116521573251944 37.0 31.0 39.0 21.5 40.5 52-53 33.00249202275249 36.5 31.0 39.0 21.5 40.0 54-55 32.647221589107346 36.0 31.0 39.0 19.5 40.0 56-57 32.149026379419695 35.5 30.5 39.0 17.5 40.0 58-59 31.68814288687173 35.0 29.5 39.0 15.5 40.0 60-61 31.31748478872681 35.0 29.0 38.0 13.0 40.0 62-63 31.133123414460123 34.5 29.0 38.0 13.0 40.0 64-65 30.65602604249247 34.0 28.5 38.0 9.5 40.0 66-67 29.749220112573333 34.0 27.0 37.5 7.5 39.5 68-69 26.19105482995085 31.0 18.5 36.0 2.0 39.0 70-71 24.838771652528933 29.5 12.5 35.0 2.0 38.0 72-73 25.8931128111622 30.5 19.5 35.0 2.0 37.5 74-75 26.68212898366894 31.0 22.5 35.0 2.0 37.0 76-77 26.370308536150308 30.5 23.0 34.0 2.0 36.0 78-79 27.537269601236723 32.0 26.0 35.0 2.0 37.0 80-81 27.5748778638814 32.0 26.0 35.0 2.0 36.0 82-83 27.25029282939591 32.0 25.5 35.0 2.0 36.0 84-85 26.896292066354846 32.0 25.0 34.5 2.0 35.5 86-87 26.456232658157603 32.0 24.5 34.0 2.0 35.0 88-89 25.980674994054226 31.5 23.5 34.0 2.0 35.0 90-91 25.39664038964642 31.0 21.5 34.0 2.0 35.0 92-93 24.82410392222927 31.0 19.5 34.0 2.0 35.0 94-95 23.948381510623115 31.0 15.0 34.0 2.0 35.0 96-97 23.277583934517203 30.0 4.5 34.0 2.0 35.0 98-99 22.411658425162518 30.0 2.0 34.0 2.0 35.0 100-101 20.98467575709529 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.31088911887310644 1101 2 0.19529045809582612 1101 3 0.36170961611139063 1101 4 0.34570276813266076 1101 5 0.5488865149548658 1101 6 0.39829417259216626 1101 7 0.3796821648479636 1101 8 0.5969774687096177 1101 9 0.6278794085818689 1101 10-11 0.6436280127577376 1101 12-13 0.7370748645076404 1101 14-15 0.7266405587141804 1101 16-17 0.7019953593065082 1101 18-19 0.5493121416409394 1101 20-21 0.7353209368692717 1101 22-23 0.6723990311847814 1101 24-25 0.6668419004857711 1101 26-27 0.7692006296126621 1101 28-29 0.6444143126446065 1101 30-31 0.39699250618718906 1101 32-33 0.7726813117659574 1101 34-35 0.90841199989854 1101 36-37 0.8725040221511406 1101 38-39 0.9259381917839278 1101 40-41 0.9267473720027297 1101 42-43 0.5396017647323035 1101 44-45 0.7115684599934937 1101 46-47 0.5246792273250307 1101 48-49 0.6340102036703925 1101 50-51 0.3846199050412267 1101 52-53 0.2869985001138744 1101 54-55 0.29907738953470187 1101 56-57 0.5801297294327696 1101 58-59 0.4707135031907903 1101 60-61 0.4127821693865137 1101 62-63 0.11637596022444896 1101 64-65 -0.06505755480602815 1101 66-67 -0.17851885090446373 1101 68-69 -6.967769253001677 1101 70-71 -8.032800623018431 1101 72-73 -5.403910485873766 1101 74-75 -3.61333987269467 1101 76-77 -0.6145056796106267 1101 78-79 -0.2008468486485704 1101 80-81 -0.3047863422325996 1101 82-83 -0.5742416783081623 1101 84-85 -0.49696776330078407 1101 86-87 -0.8516226357797763 1101 88-89 -0.6583869490745435 1101 90-91 -0.8015823378491831 1101 92-93 -0.8360159682407158 1101 94-95 -1.021022550953699 1101 96-97 -1.038244870322945 1101 98-99 -0.9658899976409714 1101 100-101 -0.8113254202237776 1102 1 0.33692109177627216 1102 2 0.3143186261894151 1102 3 0.3681154918285223 1102 4 0.33376265623318346 1102 5 0.5418047101282468 1102 6 0.5286948568327574 1102 7 0.5361599312911878 1102 8 0.5248626168812365 1102 9 0.6751472057988792 1102 10-11 0.6223394425307305 1102 12-13 0.5782166057408133 1102 14-15 0.7135698727970876 1102 16-17 0.5947511965208889 1102 18-19 0.4186688617189134 1102 20-21 0.6832893134743827 1102 22-23 0.48943002944292857 1102 24-25 0.565346331852858 1102 26-27 0.6112685322504561 1102 28-29 0.4362416441915684 1102 30-31 0.40661286348125714 1102 32-33 0.6930539856179152 1102 34-35 0.3876778833446366 1102 36-37 0.4895704685044606 1102 38-39 0.6348752319887438 1102 40-41 0.509644767171558 1102 42-43 0.4719878974943015 1102 44-45 0.5919055649852041 1102 46-47 0.6260055472870718 1102 48-49 0.6654032569803476 1102 50-51 0.48114901083993544 1102 52-53 0.20295845442206684 1102 54-55 0.02396145508312486 1102 56-57 0.36217349326746273 1102 58-59 0.3865209028208234 1102 60-61 0.45497765675164814 1102 62-63 0.23887825476047198 1102 64-65 0.11032473762726269 1102 66-67 0.021639635468975627 1102 68-69 -6.956862570289836 1102 70-71 -7.376509332025801 1102 72-73 -1.4313440437827403 1102 74-75 0.2532893331259203 1102 76-77 0.5086618882948528 1102 78-79 0.18027451880965017 1102 80-81 0.03991086425859436 1102 82-83 -0.2236703175275352 1102 84-85 -0.37719227263947275 1102 86-87 -0.3524940977188926 1102 88-89 -0.21204836790763792 1102 90-91 -0.4250296189305516 1102 92-93 -0.2666621962508202 1102 94-95 -0.07630435265463831 1102 96-97 -0.32305220033547855 1102 98-99 -0.3860943108003845 1102 100-101 -0.33725793955328953 1103 1 0.3426421393359327 1103 2 0.28684144235629816 1103 3 0.37783291147593445 1103 4 0.2794743505815731 1103 5 0.36508145180955864 1103 6 0.3711031670192497 1103 7 0.4202906495044445 1103 8 0.3949592667310142 1103 9 0.468137965959869 1103 10-11 0.5995431157918816 1103 12-13 0.5400640066817957 1103 14-15 0.6722740278903387 1103 16-17 0.6326246163950486 1103 18-19 0.6314041091538627 1103 20-21 0.5005778742135689 1103 22-23 0.6068886485688267 1103 24-25 0.6807840904833284 1103 26-27 0.5510288916405202 1103 28-29 0.5397455555344521 1103 30-31 0.46805708433041104 1103 32-33 0.6575741375367343 1103 34-35 0.4999979048853689 1103 36-37 0.7310916407736059 1103 38-39 0.9077587136507788 1103 40-41 0.851554948000306 1103 42-43 0.6244657899040291 1103 44-45 0.746967636296354 1103 46-47 0.844084170947859 1103 48-49 0.8224195825854679 1103 50-51 0.5814239385651234 1103 52-53 0.6287676489121736 1103 54-55 0.5552720383833929 1103 56-57 0.5772293291044406 1103 58-59 0.6157142592065163 1103 60-61 0.6686205908256788 1103 62-63 0.16372378505920793 1103 64-65 0.2820553651427069 1103 66-67 0.20576610222256164 1103 68-69 -6.8878904315221945 1103 70-71 -7.920779072970191 1103 72-73 -0.9776473996015689 1103 74-75 0.5287081019032023 1103 76-77 0.9455489695260724 1103 78-79 0.5972335914245797 1103 80-81 0.30386769777143385 1103 82-83 0.1216927571280948 1103 84-85 -0.016013059442943955 1103 86-87 0.10251979038268644 1103 88-89 0.12059221369204565 1103 90-91 -0.015852134660953254 1103 92-93 -0.06553910232190674 1103 94-95 0.22048721244938108 1103 96-97 -0.10188843410907467 1103 98-99 -0.1332380384802221 1103 100-101 -0.02390552188989048 1104 1 0.24326964205647528 1104 2 0.2819983447084091 1104 3 0.1396141016862522 1104 4 0.21934433358263306 1104 5 0.4868765470107306 1104 6 0.3131807006792897 1104 7 0.39019025618168257 1104 8 0.4354775565949538 1104 9 0.312541296045147 1104 10-11 0.30209980263441594 1104 12-13 0.3460465312022407 1104 14-15 0.49172341158985233 1104 16-17 0.44141810060880005 1104 18-19 0.362214243794007 1104 20-21 0.4837602145195561 1104 22-23 0.5342307149002608 1104 24-25 0.4121701620211269 1104 26-27 0.48523267977255813 1104 28-29 0.5125756123635483 1104 30-31 0.29184911047606477 1104 32-33 0.45155702835233313 1104 34-35 0.18839097159375484 1104 36-37 0.5876205852034317 1104 38-39 0.5309036776258296 1104 40-41 0.6018299360868227 1104 42-43 0.4931682693310435 1104 44-45 0.5953088171181378 1104 46-47 0.6382667459672362 1104 48-49 0.6693114970644416 1104 50-51 0.581099696227092 1104 52-53 0.3185190305205978 1104 54-55 0.35320816077576467 1104 56-57 0.5284789112434609 1104 58-59 0.6209689054649736 1104 60-61 0.6461072192743664 1104 62-63 0.3943508610281121 1104 64-65 0.3377895735539802 1104 66-67 0.22817874260606885 1104 68-69 -6.640212493870607 1104 70-71 -4.501939321766397 1104 72-73 0.36682287714686623 1104 74-75 1.0886648399559355 1104 76-77 1.3011566638581016 1104 78-79 0.8057362621785806 1104 80-81 0.4928974245792581 1104 82-83 0.3666768250669534 1104 84-85 0.23306720187131091 1104 86-87 0.14103421458804277 1104 88-89 0.4158076165811515 1104 90-91 0.4949888683476438 1104 92-93 0.012350006265048563 1104 94-95 0.3328680776171993 1104 96-97 -0.12939506654077704 1104 98-99 -0.03186425383332647 1104 100-101 0.31907868118699056 1105 1 0.07263514808794014 1105 2 0.2159655388577164 1105 3 0.1574166177991323 1105 4 0.20390450838402785 1105 5 0.3302874161948779 1105 6 0.2406307584048477 1105 7 0.29640924918336253 1105 8 0.4614519536636692 1105 9 0.39845686304425953 1105 10-11 0.3105523893679276 1105 12-13 0.3608981106553415 1105 14-15 0.4296971426577798 1105 16-17 0.3831420242326473 1105 18-19 0.38563298189835393 1105 20-21 0.6063087555547 1105 22-23 0.6450517074062816 1105 24-25 0.5608892736244329 1105 26-27 0.5339521210792171 1105 28-29 0.6155805287901615 1105 30-31 0.5019771214691104 1105 32-33 0.5695411011156679 1105 34-35 0.5843182742008182 1105 36-37 0.5546073877274225 1105 38-39 0.7122705654807078 1105 40-41 0.7205135294797884 1105 42-43 0.6358782624384105 1105 44-45 0.6663080545875317 1105 46-47 0.7119972094404119 1105 48-49 0.8682815220834286 1105 50-51 0.5160891539621417 1105 52-53 0.6027822158616942 1105 54-55 0.5937070715640829 1105 56-57 0.6813396465588824 1105 58-59 0.7300462251158599 1105 60-61 0.6642330278489617 1105 62-63 0.4442768334652776 1105 64-65 0.5762745463159042 1105 66-67 0.605273755604621 1105 68-69 -2.7039749257479393 1105 70-71 -3.0396290866567632 1105 72-73 1.1495381052109224 1105 74-75 1.3453249852122404 1105 76-77 1.2442222969902126 1105 78-79 0.9245641923759855 1105 80-81 0.7369215969642973 1105 82-83 0.676738107763228 1105 84-85 0.6379918344306503 1105 86-87 0.6392550070373915 1105 88-89 0.8779056509446654 1105 90-91 0.9184550104684774 1105 92-93 0.532309331860489 1105 94-95 0.3846466166027014 1105 96-97 0.4110512931952677 1105 98-99 0.42340919572288627 1105 100-101 0.5522646798575508 1106 1 0.06919956909636937 1106 2 0.16289623697810285 1106 3 0.09262184167617704 1106 4 0.12257753122716508 1106 5 0.2579704581442357 1106 6 0.17314641581625523 1106 7 0.3131755904078517 1106 8 0.37743541004116565 1106 9 0.3349864066821837 1106 10-11 0.37465445317093327 1106 12-13 0.15720737596340228 1106 14-15 0.2060420543821735 1106 16-17 0.17725427408574035 1106 18-19 0.21463706945307592 1106 20-21 0.25753428909452936 1106 22-23 0.26171976685809284 1106 24-25 0.3842704749844259 1106 26-27 0.4805324655241847 1106 28-29 0.5905993884969689 1106 30-31 0.5087488174607202 1106 32-33 0.4927226551326598 1106 34-35 0.42108049227365996 1106 36-37 0.4349205378590639 1106 38-39 0.5178205636437525 1106 40-41 0.39427182027923635 1106 42-43 0.36958976707848024 1106 44-45 0.3841905401969541 1106 46-47 0.4532636375632748 1106 48-49 0.5000943591715412 1106 50-51 0.3581692097293896 1106 52-53 0.2259368152030916 1106 54-55 0.17291212944905965 1106 56-57 0.4451930839367577 1106 58-59 0.7827640311727784 1106 60-61 0.8649555121411048 1106 62-63 0.39539959278648595 1106 64-65 0.4845226199417638 1106 66-67 0.5619839945551561 1106 68-69 -3.3247873617520014 1106 70-71 -3.695914618144524 1106 72-73 0.565319496700365 1106 74-75 1.229825818313497 1106 76-77 1.2403084438288516 1106 78-79 0.8367196469953306 1106 80-81 0.6025522535676302 1106 82-83 0.4131677325507148 1106 84-85 0.4041996180500895 1106 86-87 0.4082375422731097 1106 88-89 0.33700916432292516 1106 90-91 0.595225333979787 1106 92-93 0.7593940028544992 1106 94-95 0.8805804771385226 1106 96-97 0.7490517892678987 1106 98-99 0.7676049841309691 1106 100-101 0.9266111574658495 1107 1 0.023453109747265444 1107 2 0.03121720983926579 1107 3 0.11478355009997898 1107 4 -0.10069584487727212 1107 5 0.19323741999176747 1107 6 0.12017808117848006 1107 7 0.028941013594447895 1107 8 0.015349480492133694 1107 9 0.288323452416968 1107 10-11 0.04976143292171287 1107 12-13 0.04820636548448931 1107 14-15 0.03036593813100552 1107 16-17 -0.03783726169932322 1107 18-19 0.11535374271741006 1107 20-21 0.07925328660123654 1107 22-23 0.14286834791104042 1107 24-25 0.21902869561578342 1107 26-27 0.36849699954569104 1107 28-29 0.18501698053034943 1107 30-31 0.09648338250096344 1107 32-33 0.20519924214906382 1107 34-35 0.23607762143483768 1107 36-37 0.40181583183678526 1107 38-39 0.4721593338943393 1107 40-41 0.3289486149459364 1107 42-43 -0.06962693768156214 1107 44-45 0.24061287731533554 1107 46-47 0.5034678967892532 1107 48-49 0.49666001886776456 1107 50-51 0.4269552323165371 1107 52-53 0.3252571720363875 1107 54-55 0.23548544753296596 1107 56-57 0.6190471785096179 1107 58-59 0.7193495864964525 1107 60-61 0.6845778816205765 1107 62-63 0.5050436993357614 1107 64-65 0.32131578531274485 1107 66-67 0.6633412080081129 1107 68-69 -2.722470183268335 1107 70-71 -2.855970171340978 1107 72-73 0.9956491792819904 1107 74-75 1.3910354426773708 1107 76-77 0.9497676816407683 1107 78-79 0.998171778248512 1107 80-81 0.6493591019621832 1107 82-83 0.6876960969011137 1107 84-85 0.48907823681134843 1107 86-87 0.6246398227636227 1107 88-89 0.7314312241180794 1107 90-91 0.6615423144329284 1107 92-93 0.7209483090020399 1107 94-95 0.7628500236466138 1107 96-97 0.7644529892443082 1107 98-99 0.9767370892054785 1107 100-101 1.1697813685233172 1108 1 -0.03830413622851836 1108 2 0.006697280745189715 1108 3 0.09391427991673851 1108 4 0.052008592873150405 1108 5 -0.09309760879460782 1108 6 -0.15599827772400943 1108 7 0.04254344830361845 1108 8 0.16185369414986894 1108 9 0.2412022601621331 1108 10-11 0.2625168790040959 1108 12-13 0.04695233615831995 1108 14-15 0.1668875121607769 1108 16-17 0.21982910072803463 1108 18-19 0.16308135601107665 1108 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94-95 0.6241938677129468 2316 96-97 1.0953455168257022 2316 98-99 1.1433866041761434 2316 100-101 1.1651660425276553 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10.0 3 28.0 4 63.0 5 143.0 6 303.0 7 876.0 8 1899.0 9 3205.0 10 5087.0 11 7356.0 12 9724.0 13 12344.0 14 15438.0 15 19137.0 16 22664.0 17 25705.0 18 24540.0 19 22236.0 20 22810.0 21 23178.0 22 24553.0 23 27186.0 24 32163.0 25 40207.0 26 50385.0 27 62755.0 28 76876.0 29 91616.0 30 107850.0 31 124591.0 32 144607.0 33 166300.0 34 190323.0 35 210837.0 36 214335.0 37 168445.0 38 64987.0 39 3478.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.34081340862967 20.151352583624284 15.373140615669007 23.134693392077036 2 16.344668118327903 20.969335721273115 38.519156873569685 24.166839286829298 3 19.41879765933516 24.538679932416027 29.947726473196813 26.094795935052 4 13.63157143055503 17.298064201311956 35.8255162824096 33.24484808572341 5 13.97891231964484 37.51317980022198 33.69485294117647 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27.73196203441734 22.685437237885854 52-53 22.962547804299092 26.795939472841408 27.543208542257663 22.69830418060184 54-55 22.7200306555499 26.738267336818932 27.68475248270792 22.85694952492325 56-57 22.711718072197794 27.006582607473835 27.57441706764575 22.707282252682617 58-59 22.810608446604036 26.775010723474608 27.610264700700455 22.804116129220905 60-61 22.914218857549773 26.680991357117406 27.85866525666254 22.546124528670276 62-63 22.739153292078605 26.718108723992206 27.846197068367594 22.696540915561595 64-65 22.63128359436649 26.52392532093885 27.821679027700526 23.023112056994133 66-67 22.451157235597925 26.895550395687746 27.777029191633666 22.87626317708066 68-69 22.45563399742233 26.345583395054195 28.483783702148237 22.714998905375243 70-71 21.91762359561238 26.253559415610717 28.992746643622358 22.836070345154543 72-73 22.47112523608982 26.677260400696802 27.844908728515673 23.00670563469771 74-75 22.56257791176515 26.827178846123818 27.40627597317047 23.203967268940566 76-77 21.919187605945982 26.925771525095854 27.992927500424532 23.162113368533632 78-79 22.49890048081509 26.89762144576552 27.415380384082184 23.1880976893372 80-81 22.376512699915715 27.068452845483737 27.324675955311832 23.23035849928871 82-83 22.6252863619258 27.03779320897044 27.118087272040796 23.218833157062964 84-85 22.424899163205104 26.959600621631807 27.133490054312265 23.482010160850823 86-87 22.402027347589083 26.888715632491966 27.322244758766896 23.38701226115206 88-89 22.374228315348518 26.886894237303423 27.200708739660804 23.538168707687255 90-91 22.303798967415798 26.999383838736694 27.30389682977969 23.392920364067816 92-93 22.426368754060068 26.8475138630743 27.17918212445079 23.546935258414837 94-95 22.182696460831313 26.738851115297585 27.31890206377535 23.759550360095748 96-97 22.218265047281633 26.611869294263872 27.356454305162625 23.81341135329187 98-99 22.027789918956394 26.58665382179525 27.170867514467822 24.21468874478053 100-101 21.993256569492818 26.525448422271158 27.3749432596419 24.106351748594125 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 946.0 1 673.5 2 427.5 3 462.5 4 471.5 5 486.0 6 687.0 7 1318.0 8 2028.0 9 2467.5 10 2453.5 11 2207.0 12 2145.0 13 2272.0 14 2439.5 15 2566.0 16 2694.0 17 2822.5 18 3049.0 19 3368.0 20 3695.0 21 4179.5 22 4810.5 23 5553.0 24 6628.5 25 8049.0 26 10041.5 27 12527.5 28 14810.5 29 17458.0 30 20678.5 31 23885.5 32 27679.5 33 31672.0 34 35569.0 35 39925.5 36 45297.0 37 50034.5 38 52651.0 39 54421.0 40 55993.0 41 58857.5 42 62072.0 43 64422.5 44 66787.5 45 68977.5 46 70056.0 47 71466.0 48 75230.0 49 77462.0 50 77415.0 51 76783.0 52 75936.0 53 75856.0 54 74607.0 55 70468.5 56 64391.5 57 58055.5 58 52369.5 59 46703.5 60 39973.5 61 32170.0 62 25051.5 63 19421.0 64 14361.0 65 10226.0 66 7433.0 67 5677.0 68 4199.5 69 2885.0 70 2056.5 71 1545.5 72 1207.5 73 974.5 74 732.5 75 529.0 76 352.5 77 193.0 78 103.5 79 55.0 80 38.5 81 30.0 82 12.5 83 7.5 84 7.5 85 4.5 86 2.5 87 0.0 88 0.0 89 1.0 90 1.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02155343269383225 2 0.01595449500554939 3 4.954812113524656E-4 4 0.08462819089900112 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.008200214047883304 12-13 0.0 14-15 0.0 16-17 7.927699381639449E-4 18-19 1.238703028381164E-4 20-21 0.0 22-23 3.220627873791026E-4 24-25 0.0032949500554938956 26-27 0.004533653083875059 28-29 0.0015855398763278898 30-31 2.7251466624385605E-4 32-33 7.679958775963215E-4 34-35 0.003617012842872998 36-37 0.08338948787061995 38-39 0.09419097827810369 40-41 0.07697300618360552 42-43 0.08016885999682892 44-45 0.079177897574124 46-47 0.07793919454574282 48-49 0.07310825273505628 50-51 0.07263754558427143 52-53 0.05757491675915649 54-55 0.04890399556048834 56-57 0.02846539559219914 58-59 0.023312390994133503 60-61 0.013823925796733787 62-63 0.002328761693356588 64-65 0.006812866656096401 66-67 0.16502001744093864 68-69 0.7566245837957825 70-71 0.9222887268114793 72-73 0.3774328127477406 74-75 0.05621234342793721 76-77 0.21018312985571588 78-79 0.015483787854764548 80-81 0.0038152053274139843 82-83 0.0016598620580307592 84-85 1.7341842397336293E-4 86-87 0.001808506421436499 88-89 4.7070715078484224E-4 90-91 0.004930038052957031 92-93 0.005400745203741874 94-95 0.08388496908197242 96-97 0.04001010781671159 98-99 0.015558110036467416 100-101 0.01367528143332805 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2018240.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.27082649074156 #Duplication Level Percentage of deduplicated Percentage of total 1 76.86414149219054 43.25208769266864 2 13.001327491982364 14.631908869012959 3 4.343707197175186 7.332719820564908 4 1.9651961970085872 4.423328568885416 5 1.0217350704412411 2.8746938434152347 6 0.5928685307393959 2.0016721335034457 7 0.40271737799020857 1.586286779118539 8 0.28002334575994886 1.2605716082092013 9 0.20116776740596595 1.0187888881707872 >10 1.1901645672748917 12.367982874731231 >50 0.09367426343020734 3.639326575412643 >100 0.041674552737072997 4.060483164771003 >500 0.0010680972429174475 0.3783966742004856 >1k 3.5603241430581394E-4 0.444409489104023 >5k 1.7801620715290697E-4 0.727343018231559 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7625 0.37780442365625494 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6897 0.3417333914697955 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4421 0.21905224353892502 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 4.954812113524655E-5 0.0 0.0 0.0 4.954812113524655E-5 7 4.954812113524655E-5 0.0 0.0 0.0 4.954812113524655E-5 8 4.954812113524655E-5 0.0 0.0 0.0 4.954812113524655E-5 9 4.954812113524655E-5 0.0 0.0 0.0 4.954812113524655E-5 10-11 4.954812113524655E-5 0.0 0.0 0.0 4.954812113524655E-5 12-13 1.238703028381164E-4 0.0 0.0 0.0 4.954812113524655E-5 14-15 2.972887268114793E-4 0.0 0.0 0.0 7.432218170286983E-5 16-17 2.972887268114793E-4 0.0 0.0 2.4774060567623274E-5 9.90962422704931E-5 18-19 3.716109085143491E-4 0.0 0.0 4.954812113524655E-5 9.90962422704931E-5 20-21 4.45933090217219E-4 0.0 0.0 4.954812113524655E-5 9.90962422704931E-5 22-23 4.45933090217219E-4 0.0 0.0 1.4864436340573966E-4 9.90962422704931E-5 24-25 6.441255747582052E-4 0.0 0.0 6.193515141905819E-4 1.4864436340573966E-4 26-27 6.441255747582052E-4 0.0 0.0 0.0010157364832725543 1.4864436340573966E-4 28-29 7.432218170286982E-4 0.0 0.0 0.0020314729665451086 1.4864436340573966E-4 30-31 7.927699381639448E-4 0.0 0.0 0.005698033930553353 1.4864436340573966E-4 32-33 7.927699381639448E-4 0.0 0.0 0.01263477088948787 1.4864436340573966E-4 34-35 8.91866180434438E-4 0.0 0.0 0.023931742508324083 2.477406056762328E-4 36-37 0.0010405105438401777 0.0 0.0 0.04494014586966862 2.477406056762328E-4 38-39 0.0011891549072459173 0.0 0.0 0.07407444109719359 2.477406056762328E-4 40-41 0.0012139289678135406 0.0 0.0 0.10937747740605677 2.972887268114793E-4 42-43 0.0013377992706516569 0.0 0.0 0.1449530283811638 2.972887268114793E-4 44-45 0.00143689551292215 0.0 0.0 0.1894720152211828 2.972887268114793E-4 46-47 0.0015607658157602662 0.0 0.0 0.2442970112573331 3.220627873791026E-4 48-49 0.0015855398763278896 0.0 0.0 0.3042997859521167 3.4683684794672585E-4 50-51 0.0015855398763278896 0.0 0.0 0.3671268035516093 3.4683684794672585E-4 52-53 0.0016350879974631363 0.0 0.0 0.4320348422387823 3.4683684794672585E-4 54-55 0.0016846361185983828 0.0 0.0 0.5035575550975107 3.4683684794672585E-4 56-57 0.0016846361185983828 0.0 0.0 0.5904154114475979 3.4683684794672585E-4 58-59 0.0016846361185983828 0.0 0.0 0.676951205010306 3.4683684794672585E-4 60-61 0.0016846361185983828 0.0 0.0 0.7654193752972888 3.716109085143491E-4 62-63 0.0016846361185983828 0.0 0.0 0.8537884493420009 4.45933090217219E-4 64-65 0.0017341842397336294 0.0 0.0 0.9468645948945617 4.954812113524656E-4 66-67 0.001783732360868876 0.0 0.0 1.0445239416521326 5.202552719200888E-4 68-69 0.0019819248454098623 0.0 0.0 1.1610858966228 5.450293324877121E-4 70-71 0.0020810210876803553 0.0 0.0 1.305097510702394 5.450293324877121E-4 72-73 0.0022048913905184713 0.0 0.0 1.4669712224512446 7.432218170286982E-4 74-75 0.002254439511653718 0.0 0.0 1.6415540272712859 7.432218170286982E-4 76-77 0.0022792135722213415 0.0 0.0 1.8384830347233234 7.679958775963215E-4 78-79 0.002303987632788965 0.0 0.0 2.0697984382432217 7.927699381639448E-4 80-81 0.003071983510385286 0.0 2.4774060567623274E-5 2.3268540906928807 8.423180592991913E-4 82-83 0.003171079752655779 0.0 4.954812113524655E-5 2.5838849690819723 9.414143015696845E-4 84-85 0.003171079752655779 0.0 4.954812113524655E-5 2.8544177104804183 9.414143015696845E-4 86-87 0.003171079752655779 0.0 4.954812113524655E-5 3.1400130807039797 9.414143015696845E-4 88-89 0.003171079752655779 0.0 4.954812113524655E-5 3.453157206278738 9.909624227049311E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4260 0.0 58.13643 1 GTATCAA 7525 0.0 39.78417 1 GTGGTAT 1990 0.0 29.325098 1 ATCAACG 10200 0.0 29.0614 3 TATCAAC 10315 0.0 28.878202 2 TCAACGC 10320 0.0 28.680363 4 TGGTATC 1900 0.0 28.459743 2 CAACGCA 10495 0.0 28.182564 5 AACGCAG 10870 0.0 27.210304 6 ACGCAGA 12565 0.0 23.388779 7 CGCAGAG 12695 0.0 22.962584 8 GTACATG 9375 0.0 21.558859 1 GCAGAGT 13420 0.0 21.015648 9 GAGTACT 7375 0.0 20.597385 12-13 TACATGG 9635 0.0 20.479595 2 ACATGGG 9390 0.0 19.900652 3 CAGAGTA 13165 0.0 19.441978 10-11 GTACTTT 7725 0.0 19.296041 14-15 AGAGTAC 12585 0.0 19.283432 10-11 AGTACTT 7775 0.0 19.171951 12-13 >>END_MODULE