##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939503_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1037635 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.942500012046626 31.0 31.0 31.0 30.0 34.0 2 31.131197386364185 31.0 31.0 34.0 28.0 34.0 3 31.10128802517263 31.0 31.0 34.0 28.0 34.0 4 34.982935232523964 35.0 35.0 37.0 32.0 37.0 5 34.665977920945224 35.0 35.0 37.0 32.0 37.0 6 34.701844097394556 35.0 35.0 37.0 31.0 37.0 7 34.589079011405744 35.0 35.0 37.0 30.0 37.0 8 34.65231897536224 35.0 35.0 37.0 30.0 37.0 9 35.892604817686376 37.0 35.0 39.0 30.0 39.0 10-11 35.74298187705696 37.0 35.0 39.0 30.0 39.0 12-13 35.723189271757406 37.0 35.0 39.0 30.0 39.0 14-15 36.29998747150973 38.0 34.5 40.0 30.0 41.0 16-17 36.388701711102655 38.0 34.5 40.0 30.0 41.0 18-19 36.547754749984335 38.0 35.0 40.0 30.0 41.0 20-21 36.48225869404945 38.0 35.0 40.0 30.0 41.0 22-23 36.34096141706863 38.0 34.5 40.0 30.0 41.0 24-25 36.035571756928015 38.0 34.0 40.0 29.5 41.0 26-27 35.508904383526 38.0 34.0 40.0 27.5 41.0 28-29 35.252700612450425 38.0 34.0 40.0 27.0 41.0 30-31 35.008638875905305 37.0 33.0 40.0 27.0 41.0 32-33 34.67411999402488 37.0 33.0 39.5 26.0 40.5 34-35 34.476280194866206 37.0 33.0 40.0 25.0 41.0 36-37 34.27832137504999 37.0 33.0 40.0 24.5 41.0 38-39 34.11151801934206 37.0 33.0 40.0 24.0 41.0 40-41 33.911598972663796 37.0 33.0 40.0 24.0 41.0 42-43 33.578181152331986 36.5 33.0 40.0 22.5 41.0 44-45 33.31243163540166 36.0 32.0 40.0 22.0 41.0 46-47 32.826409093756475 36.0 31.0 40.0 19.5 41.0 48-49 32.374730998857984 36.0 31.0 39.0 16.0 41.0 50-51 32.07689890953948 36.0 31.0 39.0 14.0 40.0 52-53 31.682169549022536 35.0 30.0 39.0 10.5 40.0 54-55 31.531043671425884 35.0 30.0 39.0 8.5 40.0 56-57 31.161256607573954 35.0 29.5 39.0 8.0 40.0 58-59 30.768059577789877 35.0 28.5 38.5 7.0 40.0 60-61 30.374293465428593 35.0 28.0 38.0 4.0 40.0 62-63 30.014084914252123 34.0 28.0 38.0 2.0 40.0 64-65 29.556066921412636 34.0 27.0 37.5 2.0 40.0 66-67 27.871673083502387 33.0 23.0 36.5 2.0 39.0 68-69 24.46292675169978 29.5 13.0 35.0 2.0 38.0 70-71 23.78311689563286 28.5 10.5 34.0 2.0 37.0 72-73 25.475198889783016 30.0 19.5 35.0 2.0 37.0 74-75 26.053927922631754 31.0 21.0 35.0 2.0 37.0 76-77 26.011862552824454 30.5 23.0 34.0 2.0 36.0 78-79 26.785580671430708 32.0 25.0 35.0 2.0 36.0 80-81 26.616423887012292 32.0 25.0 35.0 2.0 36.0 82-83 26.20218236663181 32.0 23.5 35.0 2.0 36.0 84-85 25.840662178897205 32.0 22.5 34.5 2.0 35.0 86-87 25.435756793091983 32.0 20.5 34.0 2.0 35.0 88-89 24.8324849296718 31.0 19.0 34.0 2.0 35.0 90-91 24.27267680831892 31.0 16.5 34.0 2.0 35.0 92-93 23.781188471861494 31.0 9.0 34.0 2.0 35.0 94-95 22.50582237491989 30.0 2.0 34.0 2.0 35.0 96-97 21.64323871110747 29.0 2.0 34.0 2.0 35.0 98-99 21.313657018122942 29.0 2.0 34.0 2.0 35.0 100-101 20.168715396068947 27.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.21957089113638872 1101 2 0.14229050816322086 1101 3 0.36004636775924936 1101 4 0.21020747840559295 1101 5 0.3146275321191041 1101 6 0.41693061969223777 1101 7 0.4714219524544063 1101 8 0.5118590010561519 1101 9 0.5758575374410881 1101 10-11 0.7069396200847393 1101 12-13 0.5131901462752353 1101 14-15 0.6082291553812524 1101 16-17 0.6987202518598679 1101 18-19 0.5758913036761797 1101 20-21 0.5458279009273141 1101 22-23 0.7242408660176309 1101 24-25 0.513476006149375 1101 26-27 0.620328892036575 1101 28-29 0.5142146926830762 1101 30-31 0.6949396649813053 1101 32-33 0.9517210216799654 1101 34-35 0.7594007993226128 1101 36-37 0.8712627349346604 1101 38-39 0.8666635543050845 1101 40-41 0.9891500434840168 1101 42-43 1.2175761057542829 1101 44-45 0.8241746939164898 1101 46-47 0.9577165794622289 1101 48-49 1.0516282315334209 1101 50-51 0.9142130937023261 1101 52-53 0.9787214655456893 1101 54-55 0.9215216891904028 1101 56-57 0.8920580590077272 1101 58-59 0.8064842880140723 1101 60-61 0.8206180975344424 1101 62-63 0.5799069664685135 1101 64-65 0.2532780635099776 1101 66-67 -2.5029922830829037 1101 68-69 -7.386721323016182 1101 70-71 -10.735903623509994 1101 72-73 -9.97885477211825 1101 74-75 -8.396131360286834 1101 76-77 -3.8728549567526365 1101 78-79 -1.041312544487127 1101 80-81 -0.5310027137595554 1101 82-83 -0.3727720676514288 1101 84-85 -0.4986271231301629 1101 86-87 -0.5316370521392848 1101 88-89 -0.4951314670985134 1101 90-91 -0.5092260453552839 1101 92-93 -1.0929582081825693 1101 94-95 -4.188781083183713 1101 96-97 -1.584314255656441 1101 98-99 -0.8405070996923314 1101 100-101 -0.4940634967833013 1102 1 0.45081900329662616 1102 2 0.28309099145020156 1102 3 0.3192266628310563 1102 4 0.2272096084772599 1102 5 0.5187582235882289 1102 6 0.5289645439275859 1102 7 0.5287040123867044 1102 8 0.5326262191354729 1102 9 0.5215395330397286 1102 10-11 0.6843820434237387 1102 12-13 0.764742195733696 1102 14-15 0.6575813076166526 1102 16-17 0.6714902472702065 1102 18-19 0.6750039699531811 1102 20-21 0.8423578062705346 1102 22-23 0.7063843536079091 1102 24-25 0.5717858354690506 1102 26-27 0.7267657881730258 1102 28-29 0.6154134954494239 1102 30-31 0.513560335698898 1102 32-33 0.4551201139477641 1102 34-35 0.5178910767026679 1102 36-37 0.5924979411357967 1102 38-39 0.3529287109614714 1102 40-41 0.3812095442705328 1102 42-43 0.7042390327002792 1102 44-45 0.3658009232189059 1102 46-47 0.4751290327320561 1102 48-49 0.16726680191404597 1102 50-51 0.2987122130373301 1102 52-53 0.2640894815192709 1102 54-55 0.20655705308745098 1102 56-57 -0.053244995664108785 1102 58-59 -0.1904312269886148 1102 60-61 -0.4211948562826251 1102 62-63 0.015105714315886587 1102 64-65 -0.29509888464258793 1102 66-67 -3.3838490975469924 1102 68-69 -8.043573838109094 1102 70-71 -10.717507336032696 1102 72-73 -9.058142473013824 1102 74-75 -7.668546736030695 1102 76-77 -4.0781283551780305 1102 78-79 -1.597986180633228 1102 80-81 -1.1204716080444506 1102 82-83 -0.7268970710838651 1102 84-85 -0.6910749795248883 1102 86-87 -0.6734625588648946 1102 88-89 -0.4759435618259005 1102 90-91 -0.7815143229000938 1102 92-93 -1.1064506234798621 1102 94-95 -4.50422782943059 1102 96-97 -1.4818683998815132 1102 98-99 -0.6015209039689502 1102 100-101 -0.1764019467344653 1103 1 0.31773216292340223 1103 2 0.30498730954226616 1103 3 0.25009042062268705 1103 4 0.18358422615506242 1103 5 0.24762887285842794 1103 6 0.3186544273919125 1103 7 0.3728048292979267 1103 8 0.21390075550932153 1103 9 0.6052044058571013 1103 10-11 0.6183876185524682 1103 12-13 0.3988499815558342 1103 14-15 0.6552699521634793 1103 16-17 0.4622847031144133 1103 18-19 0.6228439947315323 1103 20-21 0.676802809948569 1103 22-23 0.5442983262742871 1103 24-25 0.6205608557950413 1103 26-27 0.5619282025134922 1103 28-29 0.5562296323498117 1103 30-31 0.8030798680075435 1103 32-33 0.7935501731835117 1103 34-35 0.9062653846510997 1103 36-37 0.8074071517130506 1103 38-39 0.9522333685169215 1103 40-41 0.8138426311109157 1103 42-43 1.1220082068843382 1103 44-45 0.9614070248875706 1103 46-47 0.7612446392208909 1103 48-49 0.5397128896448891 1103 50-51 0.8021560165140436 1103 52-53 0.6017994199605567 1103 54-55 0.5337013482595445 1103 56-57 0.9907268963952056 1103 58-59 0.8967409438271368 1103 60-61 0.7070919531176614 1103 62-63 0.6337893644765593 1103 64-65 0.3570034034831586 1103 66-67 -2.813315975862068 1103 68-69 -7.1193893942669 1103 70-71 -10.724446048332824 1103 72-73 -8.41022424156855 1103 74-75 -6.559693896199494 1103 76-77 -3.3291353628051183 1103 78-79 -0.7946337473218321 1103 80-81 -0.14277057432266815 1103 82-83 0.11112182240794866 1103 84-85 -0.255236967987468 1103 86-87 0.11746082183121942 1103 88-89 0.017316339835023342 1103 90-91 0.058795406473869605 1103 92-93 0.14601133788140075 1103 94-95 0.8584435864198099 1103 96-97 1.0280262921313401 1103 98-99 1.0558173592235853 1103 100-101 0.8518778937549065 1104 1 0.4205040417654722 1104 2 0.2799792994085166 1104 3 0.43051886963725394 1104 4 0.27483268932707716 1104 5 0.4097688825451016 1104 6 0.5718826975755817 1104 7 0.5015213450760285 1104 8 0.31003875334844366 1104 9 0.5318680920088852 1104 10-11 0.6542853123790735 1104 12-13 0.49106972001565197 1104 14-15 0.6922395136552311 1104 16-17 0.7426032456426341 1104 18-19 0.8953919623929423 1104 20-21 0.7718991178357584 1104 22-23 0.7307883974340754 1104 24-25 0.8299982836106921 1104 26-27 0.9207490363125359 1104 28-29 1.0157371022652413 1104 30-31 1.0539833365817373 1104 32-33 0.7132070414074931 1104 34-35 0.7200472854744646 1104 36-37 0.9159422286475944 1104 38-39 0.8954821297404223 1104 40-41 1.208298128298054 1104 42-43 1.0161537305235342 1104 44-45 0.9524323220076454 1104 46-47 0.9777298670439976 1104 48-49 0.9441169729502761 1104 50-51 0.7613024715641536 1104 52-53 0.6446037119019863 1104 54-55 0.5264180875762641 1104 56-57 0.5768917188682856 1104 58-59 0.8161355267413306 1104 60-61 0.44556741975452496 1104 62-63 0.5790998968710284 1104 64-65 0.4479705805630019 1104 66-67 -2.137920126229041 1104 68-69 -6.725292303994554 1104 70-71 -7.894507521452304 1104 72-73 -7.203984533659003 1104 74-75 -6.148950630300309 1104 76-77 -2.9877717772457117 1104 78-79 -0.42687211399967495 1104 80-81 0.25899687100351443 1104 82-83 0.33629934932572425 1104 84-85 0.5080714035337088 1104 86-87 0.5766635340997652 1104 88-89 0.6044446137443416 1104 90-91 0.2136353900810377 1104 92-93 0.38082524198333445 1104 94-95 1.156969721809702 1104 96-97 1.243199720841151 1104 98-99 0.6896122997995668 1104 100-101 0.6300806432798893 1105 1 0.5203825500586028 1105 2 0.51080327142434 1105 3 0.5713575439103593 1105 4 0.5049362213903237 1105 5 0.6152211897137008 1105 6 0.6384727716444445 1105 7 0.6975338346582589 1105 8 0.45737752739734816 1105 9 0.8765832027600169 1105 10-11 0.9262576304643204 1105 12-13 0.8329841387922841 1105 14-15 0.9393192374523451 1105 16-17 1.007126126580765 1105 18-19 0.8317049238560088 1105 20-21 1.003761565160005 1105 22-23 1.1644338235941163 1105 24-25 1.175654661631846 1105 26-27 1.2303856176415522 1105 28-29 1.361473080831054 1105 30-31 1.1648837691391662 1105 32-33 1.3696386186867855 1105 34-35 1.337283848022146 1105 36-37 1.3414214459358078 1105 38-39 1.1614337854317611 1105 40-41 1.235273542367608 1105 42-43 1.4293168727568784 1105 44-45 1.1128714382288436 1105 46-47 1.3812833527408444 1105 48-49 1.201246540627615 1105 50-51 1.4935757604092181 1105 52-53 1.3700632674888453 1105 54-55 1.3950786561809245 1105 56-57 1.6333527153350467 1105 58-59 1.4835813250064085 1105 60-61 1.5995383497823426 1105 62-63 1.6806111333866234 1105 64-65 1.9129742194450436 1105 66-67 2.3092346495101808 1105 68-69 -4.387877333516453 1105 70-71 -4.420246375908764 1105 72-73 -0.6635848728967204 1105 74-75 1.7162717083270103 1105 76-77 1.7391403965754648 1105 78-79 1.5865961514274218 1105 80-81 1.5999282047124765 1105 82-83 1.8423405537956796 1105 84-85 1.8798635382662532 1105 86-87 1.8361862536358657 1105 88-89 1.9188179777147134 1105 90-91 1.9483032980760129 1105 92-93 2.1209056201923 1105 94-95 2.6384264631321415 1105 96-97 2.6529473761099 1105 98-99 2.474305298473137 1105 100-101 2.1919225632010075 1106 1 0.13073031198093332 1106 2 0.22816488669634793 1106 3 0.26979502531425936 1106 4 0.13827768741748514 1106 5 0.37458841654444797 1106 6 0.3456139710779311 1106 7 0.025342886941565723 1106 8 0.2818310996335214 1106 9 0.40151536419200795 1106 10-11 0.36139332247721967 1106 12-13 0.43572944364928645 1106 14-15 0.7878729816652381 1106 16-17 0.6019398234256741 1106 18-19 0.3660117505582221 1106 20-21 0.5733366368665642 1106 22-23 0.5418520602674093 1106 24-25 0.5171169695713402 1106 26-27 0.6336174708246105 1106 28-29 0.6837527691306846 1106 30-31 0.24511514923747768 1106 32-33 0.4339774009249808 1106 34-35 0.5728562508166206 1106 36-37 0.7323144157178945 1106 38-39 0.6012992091847806 1106 40-41 0.5848258970800089 1106 42-43 0.7570809224813573 1106 44-45 0.6428874011199568 1106 46-47 0.4211638021416064 1106 48-49 0.5116779106096416 1106 50-51 0.3249660493777178 1106 52-53 0.40490938105298824 1106 54-55 0.45819158359365986 1106 56-57 0.5467934547754396 1106 58-59 0.8793613495235029 1106 60-61 0.6424321110143865 1106 62-63 0.5543023399610121 1106 64-65 0.5292147131193907 1106 66-67 1.3087102395272012 1106 68-69 -5.239289670019556 1106 70-71 -6.047411333717935 1106 72-73 -2.1236099553146275 1106 74-75 0.5584559168175396 1106 76-77 0.7450390790790671 1106 78-79 0.8084059535054244 1106 80-81 0.9511560679165996 1106 82-83 1.0005929817023222 1106 84-85 0.7143690456868406 1106 86-87 0.756106989624822 1106 88-89 0.8503095782288597 1106 90-91 0.544240199281635 1106 92-93 0.9337165469623514 1106 94-95 1.758652100755171 1106 96-97 1.7268103362517628 1106 98-99 1.4156394421004777 1106 100-101 1.456143371789132 1107 1 0.07771520331809612 1107 2 0.024222585521300743 1107 3 -0.0034202176751847446 1107 4 -0.09367448491493491 1107 5 -0.03151081261798083 1107 6 0.060082117802025437 1107 7 -0.009791532095846378 1107 8 -0.03976251923628382 1107 9 0.3266706408247231 1107 10-11 0.173892552255829 1107 12-13 0.20083425537637112 1107 14-15 0.30130371719585725 1107 16-17 -0.08279238600059813 1107 18-19 0.0173529370688712 1107 20-21 0.20913475567783024 1107 22-23 0.18387664881514354 1107 24-25 0.21799533004727323 1107 26-27 0.3479958867564861 1107 28-29 0.21257637918915862 1107 30-31 0.22487296082283592 1107 32-33 0.3734276014906328 1107 34-35 0.17407219932886164 1107 36-37 0.2808098652499069 1107 38-39 0.1812712963615155 1107 40-41 0.2071597757300907 1107 42-43 0.2498350647320038 1107 44-45 0.3679264513445517 1107 46-47 0.16419406876143938 1107 48-49 0.15620379312931476 1107 50-51 -0.10971956165431962 1107 52-53 0.13561995650079695 1107 54-55 0.046607607895683856 1107 56-57 0.050817861550921606 1107 58-59 -0.0779167805021892 1107 60-61 0.03272716742539927 1107 62-63 0.0037099626880916503 1107 64-65 0.26814278060311025 1107 66-67 1.0395671570395706 1107 68-69 -5.202177707557066 1107 70-71 -5.516000164891565 1107 72-73 -2.0539715434495207 1107 74-75 0.29643237577090176 1107 76-77 0.20211887709620768 1107 78-79 0.42374275591591015 1107 80-81 0.39764570689842316 1107 82-83 0.4735431915106325 1107 84-85 0.4239620761075038 1107 86-87 0.3977815125496882 1107 88-89 0.11567202901171214 1107 90-91 -0.04087927746076048 1107 92-93 -0.016019960299296088 1107 94-95 0.9323974871290304 1107 96-97 1.2699504364421657 1107 98-99 0.944634041308074 1107 100-101 0.6633048132738715 1108 1 -0.11909330732019896 1108 2 -0.03694762724465761 1108 3 0.10739538516169489 1108 4 -0.0537808678936571 1108 5 0.04207074766570429 1108 6 0.2905785717027314 1108 7 0.35457017003181335 1108 8 0.16945733891974157 1108 9 0.37188340678216747 1108 10-11 0.1322258855891647 1108 12-13 0.299238510695524 1108 14-15 0.15103775974905176 1108 16-17 0.02226080548876297 1108 18-19 0.2004203129553943 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0.18507243856029731 2316 94-95 0.5906362774497111 2316 96-97 0.6980624036436929 2316 98-99 -0.015709435518576953 2316 100-101 -0.2880585499833579 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 15.0 4 59.0 5 171.0 6 415.0 7 848.0 8 1514.0 9 2463.0 10 3849.0 11 5652.0 12 7239.0 13 8998.0 14 10787.0 15 13804.0 16 16535.0 17 18356.0 18 15947.0 19 12773.0 20 12281.0 21 12601.0 22 13226.0 23 14901.0 24 17734.0 25 21263.0 26 26053.0 27 32466.0 28 39318.0 29 46378.0 30 53932.0 31 62638.0 32 71573.0 33 82810.0 34 95906.0 35 107079.0 36 99169.0 37 76853.0 38 30195.0 39 1831.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.8266744813306 20.1071752881818 15.102673501926997 17.963476728560604 2 14.901157920812597 25.279744916204237 36.91129225813354 22.90780490484962 3 23.749198899420314 28.280850202624237 30.719183527926486 17.25076737002896 4 11.656700092036216 17.8897203737345 39.22670303141278 31.226876502816502 5 10.915505852738056 42.72797537474678 32.64156725781923 13.714951514695933 6 25.927517865145262 38.166889127679774 17.60888944571068 18.29670356146429 7 24.45011974345507 37.932028121642006 20.373349010008337 17.244503124894592 8 24.717362078187417 31.212613298510554 19.67156080895498 24.39846381434705 9 25.348508868725517 20.17038746765481 23.028617962963853 31.45248570065582 10-11 24.417063803746018 27.05474468382427 25.68311593190284 22.84507558052687 12-13 30.17303772521166 25.640952743498435 25.815966115252476 18.370043416037433 14-15 24.860861478265477 26.62149985303117 30.143306654073925 18.37433201462942 16-17 18.415440156047616 30.34076628322951 30.95220656263492 20.291586998087954 18-19 17.528214849677408 31.0019048426758 33.3281933581562 18.141686949490587 20-21 20.113103409726644 27.29901521200384 31.69256612034392 20.8953152579256 22-23 18.648705951514742 27.413444997518393 31.978826851445834 21.959022199521026 24-25 20.766984536951817 27.55482419155098 32.6639907096426 19.0142005618546 26-27 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32.779674113260185 18.705713653181384 52-53 19.294317680972682 30.55209700612316 30.83043120583518 19.323154107068973 54-55 17.505153098247586 28.80291418253178 32.110877798644644 21.58105492057599 56-57 20.2552470693489 29.227891112194083 29.73667884768616 20.780182970770856 58-59 21.527523755959773 28.181987736589697 29.737830206274552 20.552658301175978 60-61 19.88602876508673 28.32211692533077 32.45818845974984 19.333665849832656 62-63 20.933458877771503 28.180903495905028 31.538221342425988 19.34741628389748 64-65 19.82728128737653 30.49225941442757 30.97241377316321 18.708045525032695 66-67 19.206880400111487 28.76077119011489 30.855249914836953 21.17709849493667 68-69 18.820522311390388 29.971918585757606 30.866773823905586 20.34078527894642 70-71 19.84888540590049 29.716740350669085 29.923256499084584 20.511117744345842 72-73 20.03796922164062 28.042671645476286 30.668046662810795 21.2513124700723 74-75 18.468468033729067 31.528926138288394 30.011658667418175 19.990947160564364 76-77 19.786802147882334 28.648118000514707 30.14404035905992 21.42103949254304 78-79 19.263789194177587 28.5817207487886 31.71701400183607 20.437476055197738 80-81 19.954916014452316 30.053516753259146 30.16217898862685 19.82938824366169 82-83 19.09100984450216 29.27301025890607 30.78519903434252 20.85078086224925 84-85 18.859241862139317 29.245124367009772 30.331971421536196 21.563662349314715 86-87 19.352581703094266 29.95049290256922 30.142709966664388 20.554215427672126 88-89 19.744175400554152 28.79518130345741 29.40257800265028 22.058065293338153 90-91 19.06058858139942 28.145719458382906 30.49764527925836 22.296046680959314 92-93 19.421040178986612 30.448155610028532 30.262586622552874 19.868217588431982 94-95 18.77719767868069 29.97900729167019 29.875130329127224 21.368664700521897 96-97 16.821114652757654 29.623490775620592 30.777117743649185 22.77827682797257 98-99 18.816157519233993 29.947922188829533 28.63378397395242 22.60213631798405 100-101 18.863553783447497 28.81845755277531 29.87150610230358 22.446482561473612 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 9432.0 1 6200.0 2 3221.5 3 3637.0 4 3302.0 5 3060.5 6 4814.5 7 8487.5 8 11295.5 9 11799.5 10 9947.5 11 7289.5 12 5845.5 13 4999.5 14 4438.0 15 3981.0 16 3544.5 17 3396.0 18 3404.0 19 3425.0 20 3669.5 21 3895.5 22 4088.0 23 4436.5 24 4946.0 25 5697.0 26 6751.5 27 7745.0 28 8640.0 29 9772.0 30 11047.0 31 12533.5 32 14662.5 33 17008.0 34 19233.5 35 21174.0 36 23726.0 37 25966.5 38 26345.0 39 26054.5 40 25667.0 41 25180.5 42 24665.5 43 24786.5 44 26171.5 45 28823.0 46 36226.0 47 57608.5 48 94482.0 49 88488.5 50 48217.0 51 29088.5 52 20225.0 53 16650.5 54 15177.5 55 14066.5 56 13049.0 57 12053.0 58 11371.5 59 10816.5 60 10217.5 61 9369.0 62 7628.0 63 5337.5 64 3561.5 65 2511.5 66 1847.0 67 1385.0 68 1001.5 69 747.0 70 596.0 71 468.5 72 417.5 73 422.5 74 344.0 75 256.0 76 198.0 77 127.5 78 68.0 79 43.0 80 26.0 81 15.0 82 12.0 83 9.0 84 7.0 85 4.0 86 2.0 87 1.0 88 0.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02592433755607704 2 0.016576156355558553 3 0.0 4 0.0 5 9.637300206720088E-5 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.8911900620160266E-4 18-19 0.0015901545341088148 20-21 4.818650103360044E-5 22-23 0.0 24-25 0.0 26-27 3.373055072352031E-4 28-29 0.018599989398969775 30-31 0.005445074616796851 32-33 0.009589113705686488 34-35 0.0106974032294593 36-37 0.09589113705686489 38-39 0.0981077161044105 40-41 0.11449112645583466 42-43 0.09911963262611613 44-45 0.09367455800931927 46-47 0.09439735552482328 48-49 0.0972403590858057 50-51 0.09507196653929369 52-53 0.07247249755453507 54-55 0.061437788817840566 56-57 0.03522433225556193 58-59 0.015275120827651343 60-61 0.008914502691216082 62-63 0.002023833043411219 64-65 0.005011396107494447 66-67 0.4166204879365094 68-69 0.8005223416712042 70-71 0.8812347309024849 72-73 0.27461486939048896 74-75 0.14417401109253253 76-77 0.014263204305945732 78-79 0.008914502691216082 80-81 0.0015419680330752141 82-83 0.0 84-85 4.818650103360044E-5 86-87 5.782380124032053E-4 88-89 9.63730020672009E-4 90-91 0.0037103605795872343 92-93 0.001493781532041614 94-95 0.2192967662039156 96-97 0.2250309598269141 98-99 0.014359577308012935 100-101 0.007324348157107268 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1037635.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.752579147705035 #Duplication Level Percentage of deduplicated Percentage of total 1 77.75086721011876 31.68548369783069 2 11.856516595817027 9.663672619742233 3 4.182043846479596 5.112872185584977 4 1.921612877416443 3.1324272351265128 5 1.0807753527058725 2.2022191551017447 6 0.6649053208518755 1.6257964028247762 7 0.4484993337869763 1.2794253218492713 8 0.3170689861786527 1.0337103163622512 9 0.22721774910338494 0.8333738373689172 >10 1.337455330041403 10.202116802136906 >50 0.11914891248567512 3.3656219151458275 >100 0.07553972017995014 5.7706639285612304 >500 0.005719095532776717 1.6511889127821415 >1k 0.010008417182359257 10.407549666045691 >5k 7.148869415970897E-4 2.0914930079744156 >10k+ 0.001906365177592239 9.942384995562456 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 18728 1.8048735827145383 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14891 1.4350903737826886 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13782 1.3282127144901628 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 11735 1.1309371792586025 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 11193 1.0787030121381798 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 11115 1.0711859179769379 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 10554 1.017120663817238 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10383 1.000640880463747 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 7520 0.7247249755453506 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 7483 0.7211591744688644 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 6534 0.6297011955070907 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 4879 0.47020387708587313 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 4681 0.45112202267656737 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 4614 0.44466503153806497 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 4373 0.4214391380398695 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 4298 0.4142111628848294 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 4292 0.41363292487242626 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4185 0.40332101365123574 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4142 0.39917697456234613 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 4005 0.38597387327913957 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 3682 0.3548453936114337 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 3624 0.34925575949153603 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 3588 0.3457863314171168 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 3576 0.3446298553923104 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3270 0.3151397167597469 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 2774 0.2673387077344153 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 2640 0.25442472545741035 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2617 0.2522081464098647 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 2378 0.22917499891580373 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 2367 0.22811489589306452 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2343 0.2258019438434517 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 2311 0.22271800777730125 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 2289 0.22059780173182283 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1984 0.19120403610132658 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 1941 0.18705999701243695 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1909 0.18397606094628652 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1832 0.17655533978711205 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 1796 0.1730859117126928 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 1785 0.1720258086899536 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 1691 0.1629667464956367 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1690 0.16287037349356953 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 1653 0.15930457241708307 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 1617 0.15583514434266385 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1540 0.14841442318348938 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1513 0.14581235212767496 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 1414 0.13627142492302205 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 1380 0.13299474285273724 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 1289 0.12422479966462195 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 1096 0.10562481026565218 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 1084 0.10446833424084577 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 4.818650103360045E-5 0.0 14-15 0.0 0.0 0.0 2.4093250516800223E-4 0.0 16-17 0.0 0.0 0.0 9.637300206720089E-4 0.0 18-19 0.0 0.0 0.0 0.0012046625258400113 0.0 20-21 0.0 0.0 0.0 0.0022647655485792213 0.0 22-23 0.0 0.0 0.0 0.005493261117830451 0.0 24-25 0.0 0.0 0.0 0.007998959171577674 0.0 26-27 0.0 0.0 0.0 0.010119165217056095 0.0 28-29 0.0 0.0 0.0 0.013829525796643328 0.0 30-31 0.0 0.0 0.0 0.02471967503023703 0.0 32-33 0.0 0.0 0.0 0.04356059693437481 0.0 34-35 0.0 0.0 0.0 0.0747854496041479 0.0 36-37 0.0 0.0 0.0 0.1187315385467915 0.0 38-39 9.63730020672009E-5 0.0 0.0 0.17197762218892 0.0 40-41 9.63730020672009E-5 0.0 0.0 0.23028328843957654 0.0 42-43 9.63730020672009E-5 0.0 0.0 0.28834802218506506 0.0 44-45 9.63730020672009E-5 0.0 0.0 0.35749565116828175 0.0 46-47 9.63730020672009E-5 0.0 0.0 0.45015829265589535 0.0 48-49 9.63730020672009E-5 0.0 0.0 0.5458084972075923 0.0 50-51 9.63730020672009E-5 0.0 0.0 0.6506623234567068 0.0 52-53 9.63730020672009E-5 0.0 0.0 0.7469871390228742 0.0 54-55 9.63730020672009E-5 0.0 0.0 0.8490461482120399 0.0 56-57 9.63730020672009E-5 0.0 0.0 0.9760175784355771 0.0 58-59 9.63730020672009E-5 0.0 0.0 1.112529935863767 0.0 60-61 9.63730020672009E-5 0.0 0.0 1.2616671565627606 0.0 62-63 9.63730020672009E-5 0.0 0.0 1.3890722652956 0.0 64-65 9.63730020672009E-5 0.0 0.0 1.500864947693553 0.0 66-67 9.63730020672009E-5 0.0 0.0 1.6296674649563672 0.0 68-69 9.63730020672009E-5 0.0 0.0 1.7842015737711239 0.0 70-71 9.63730020672009E-5 0.0 0.0 1.982103533516121 0.0 72-73 9.63730020672009E-5 0.0 0.0 2.1969189551239117 0.0 74-75 9.63730020672009E-5 0.0 0.0 2.4368877302712417 0.0 76-77 9.63730020672009E-5 0.0 0.0 2.6855300756046203 0.0 78-79 9.63730020672009E-5 0.0 0.0 2.999802435345762 0.0 80-81 9.63730020672009E-5 0.0 0.0 3.3462633777773494 0.0 82-83 9.63730020672009E-5 0.0 0.0 3.674943501327538 0.0 84-85 1.4455950310080136E-4 0.0 0.0 4.052388363923731 0.0 86-87 1.927460041344018E-4 0.0 0.0 4.427616647472377 0.0 88-89 1.927460041344018E-4 0.0 0.0 4.78458224712929 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 50 2.860438E-5 47.405025 2 TACCTGG 515 0.0 34.978466 2 CGTCACT 165 7.8216544E-11 31.617065 1 GCTATCG 60 0.0039896728 31.603352 2 CGCATAG 435 0.0 29.846739 66-67 CATATAG 185 2.9831426E-10 28.181341 3 GGGACCG 85 6.489483E-4 27.879934 5 ATAACGC 105 7.009109E-5 27.083364 3 ACCTGGG 675 0.0 26.68213 3 ACGCATA 505 0.0 25.233463 64-65 AACGCAT 510 0.0 24.986078 64-65 TAGTAAG 740 0.0 23.894262 70-71 GTACATG 10800 0.0 23.844538 1 CATGGGT 740 0.0 23.697947 4 ATGGGCG 140 1.8097351E-5 23.697945 5 TAACGCA 120 1.736298E-4 23.697945 4 ATAGTAA 750 0.0 23.684418 68-69 TACATGG 10635 0.0 23.579935 2 GCGTTTA 530 0.0 23.548689 62-63 TCGTTAG 145 2.373983E-5 22.880775 9 >>END_MODULE