##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939501_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 739223 Sequences flagged as poor quality 0 Sequence length 101 %GC 32 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.89119791997814 31.0 31.0 31.0 30.0 34.0 2 30.975464778558027 31.0 31.0 33.0 28.0 34.0 3 30.840747649897256 31.0 30.0 34.0 27.0 34.0 4 34.84316640580718 35.0 35.0 37.0 32.0 37.0 5 34.54371279032173 35.0 35.0 37.0 30.0 37.0 6 34.63206096130667 35.0 35.0 37.0 31.0 37.0 7 34.48874155701324 35.0 35.0 37.0 30.0 37.0 8 34.59093940529448 35.0 35.0 37.0 30.0 37.0 9 35.695788686228646 37.0 35.0 39.0 30.0 39.0 10-11 35.58509340212629 37.0 35.0 39.0 30.0 39.0 12-13 35.49826507021562 37.0 35.0 39.0 30.0 39.0 14-15 35.82748778108906 38.0 34.0 40.0 27.0 41.0 16-17 35.9615068795208 38.0 34.0 40.0 28.5 41.0 18-19 36.330317779614546 38.0 35.0 40.0 30.0 41.0 20-21 36.43454749108185 38.0 35.0 40.0 30.0 41.0 22-23 36.247919775223444 38.0 35.0 40.0 30.0 41.0 24-25 35.92323493722463 38.0 34.0 40.0 29.5 41.0 26-27 35.32066507670892 37.5 33.5 40.0 27.5 41.0 28-29 34.85594536425409 37.0 33.0 40.0 26.5 41.0 30-31 34.27523223709219 35.5 33.0 39.0 26.0 40.0 32-33 33.477582542750966 35.0 33.0 38.0 24.5 40.0 34-35 32.81081689828374 35.0 32.0 38.0 22.0 40.0 36-37 32.176995980915095 35.0 31.0 38.0 20.0 40.0 38-39 31.81960450364775 35.0 31.0 38.0 18.0 40.0 40-41 31.295234996746586 35.0 31.0 38.0 15.5 40.0 42-43 30.68779853981816 35.0 30.0 38.0 10.0 40.0 44-45 30.070610627645515 35.0 28.0 38.0 3.5 40.0 46-47 29.15854349769961 34.0 24.5 37.5 2.0 40.0 48-49 28.478002578382977 34.0 23.5 37.0 2.0 40.0 50-51 28.108493648060193 33.0 23.0 37.0 2.0 40.0 52-53 27.63327169203339 33.0 23.0 36.5 2.0 40.0 54-55 27.453531613599687 33.0 23.0 36.0 2.0 40.0 56-57 27.11464808319005 33.0 22.0 36.0 2.0 40.0 58-59 26.75829418186393 33.0 21.5 35.0 2.0 39.0 60-61 26.43544370237398 33.0 20.5 35.0 2.0 39.0 62-63 25.950914000240793 33.0 19.5 35.0 2.0 39.0 64-65 25.42552923813247 33.0 15.5 35.0 2.0 38.5 66-67 24.063261018664193 31.0 8.5 35.0 2.0 37.5 68-69 21.22072094618268 26.5 2.0 33.5 2.0 36.0 70-71 20.7267177834023 25.5 2.0 33.0 2.0 35.0 72-73 22.050827017016516 29.0 2.0 34.0 2.0 35.0 74-75 22.385415497082747 29.0 2.0 34.0 2.0 35.0 76-77 22.156999985119512 29.0 2.0 33.5 2.0 35.0 78-79 22.75639286115286 30.5 2.0 34.0 2.0 35.0 80-81 22.609855889224228 31.0 2.0 34.0 2.0 35.0 82-83 22.23910781996772 30.0 2.0 34.0 2.0 35.0 84-85 21.907998668872587 30.0 2.0 34.0 2.0 35.0 86-87 21.407568487452366 29.0 2.0 34.0 2.0 35.0 88-89 20.816873257460877 29.0 2.0 34.0 2.0 35.0 90-91 20.272160092421366 28.5 2.0 33.5 2.0 35.0 92-93 19.8269284099656 27.5 2.0 33.0 2.0 35.0 94-95 18.78608687770808 25.0 2.0 33.0 2.0 35.0 96-97 18.019635482121092 24.0 2.0 33.0 2.0 35.0 98-99 17.703483928395084 24.0 2.0 33.0 2.0 35.0 100-101 16.955372059581478 19.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.49639736423692327 1101 2 0.14950675900996657 1101 3 0.32580021496352884 1101 4 0.29828901075094194 1101 5 0.40514284611639795 1101 6 0.5185546373892791 1101 7 0.4055573188148358 1101 8 0.35825733911879354 1101 9 0.4714725966187032 1101 10-11 0.8601352340677195 1101 12-13 0.6834152078914428 1101 14-15 0.6973262573739873 1101 16-17 0.9149821900204245 1101 18-19 0.9884176529660564 1101 20-21 0.7928039067890253 1101 22-23 0.8478020114363645 1101 24-25 0.6639605252281342 1101 26-27 0.8300899794534473 1101 28-29 0.8199974458476191 1101 30-31 0.752203716774666 1101 32-33 0.5871490591362161 1101 34-35 0.6317023757729672 1101 36-37 0.7513617371709742 1101 38-39 0.8741346210904517 1101 40-41 0.9765870066018429 1101 42-43 0.7404129939317983 1101 44-45 0.5659498333887676 1101 46-47 0.339987269677426 1101 48-49 0.37931754185841626 1101 50-51 0.429395533439763 1101 52-53 0.3653813882614685 1101 54-55 0.3978257842772699 1101 56-57 0.4408781956492014 1101 58-59 0.29285338637709657 1101 60-61 0.18160681670121193 1101 62-63 0.3494142705060135 1101 64-65 0.1582982854957642 1101 66-67 -2.285190366013339 1101 68-69 -6.7397790110367275 1101 70-71 -9.273590879958274 1101 72-73 -9.082688778857184 1101 74-75 -7.2848070101838935 1101 76-77 -3.030912283928661 1101 78-79 -0.9656469203007099 1101 80-81 -0.847061707611342 1101 82-83 -0.5697481660572592 1101 84-85 -0.5938683795456967 1101 86-87 -0.42203673969856226 1101 88-89 -0.571397824332248 1101 90-91 -0.8814467635813266 1101 92-93 -1.5313346176546858 1101 94-95 -3.8238706234698423 1101 96-97 -1.9407142395359038 1101 98-99 -1.4926888550352473 1101 100-101 -1.2285110694928125 1102 1 0.5020747021962357 1102 2 0.3871126714699997 1102 3 0.5165537525472175 1102 4 0.35537421383047274 1102 5 0.36782796891034053 1102 6 0.7013584681587304 1102 7 0.6438478092737512 1102 8 0.6017018775483507 1102 9 0.8586785148134197 1102 10-11 0.8122165662350014 1102 12-13 0.8113470654992199 1102 14-15 0.9031960656921498 1102 16-17 0.8351643379466083 1102 18-19 0.898650122751441 1102 20-21 1.0416193359584938 1102 22-23 1.0247994738383355 1102 24-25 0.9325839141401175 1102 26-27 0.8993900611727099 1102 28-29 0.7179989081922429 1102 30-31 0.709451641535594 1102 32-33 0.5034710473514394 1102 34-35 0.8705502059115986 1102 36-37 1.404750219056531 1102 38-39 1.2921071232767964 1102 40-41 1.1130115739915531 1102 42-43 1.453286286070984 1102 44-45 1.4265987846582853 1102 46-47 1.511814555307737 1102 48-49 0.9613468317610732 1102 50-51 1.0396824827308109 1102 52-53 1.0266030911761241 1102 54-55 1.0439652661485006 1102 56-57 0.9267565343110142 1102 58-59 1.2259314067638982 1102 60-61 0.8891504361475278 1102 62-63 0.7604800616774661 1102 64-65 1.0665988833366562 1102 66-67 -1.3628129090593148 1102 68-69 -6.332826064125573 1102 70-71 -8.645332862152078 1102 72-73 -6.521098264025856 1102 74-75 -4.928733262137271 1102 76-77 -2.1555287768821643 1102 78-79 0.10373738960165468 1102 80-81 0.39754410449171473 1102 82-83 0.619014360644865 1102 84-85 0.8043355888561905 1102 86-87 0.6842914849863888 1102 88-89 0.6410973370274498 1102 90-91 0.46764142153429944 1102 92-93 0.1770118576646631 1102 94-95 -2.201521123104257 1102 96-97 -0.09810983243062665 1102 98-99 0.4953149011833027 1102 100-101 0.46459727303991016 1103 1 0.3905818380415873 1103 2 0.2913965813228927 1103 3 0.44361813647560666 1103 4 0.35739758123554566 1103 5 0.31202318696721676 1103 6 0.5309614144837198 1103 7 0.6468682729149222 1103 8 0.5413029017601119 1103 9 1.0425835624200488 1103 10-11 0.9943942861968296 1103 12-13 0.6820480533281241 1103 14-15 0.996228920098936 1103 16-17 0.8551465927227184 1103 18-19 0.8181213013500823 1103 20-21 0.9733302569939681 1103 22-23 0.9931841436124174 1103 24-25 1.0411747305577137 1103 26-27 1.3214912741634421 1103 28-29 1.105150267744115 1103 30-31 1.2969455491231372 1103 32-33 1.302163152271369 1103 34-35 1.62221575134555 1103 36-37 1.4302714206518914 1103 38-39 1.249765714779688 1103 40-41 1.3405177007274638 1103 42-43 1.2625980665656513 1103 44-45 1.1367192256459795 1103 46-47 1.4618644959281681 1103 48-49 1.2675145841928845 1103 50-51 1.024275543902064 1103 52-53 0.8205139394458776 1103 54-55 0.7433373186221885 1103 56-57 0.9061676125478897 1103 58-59 1.0637762716324772 1103 60-61 1.1177471905220493 1103 62-63 1.0000869208758054 1103 64-65 0.632619182225266 1103 66-67 -2.0101806436429364 1103 68-69 -6.649453888087461 1103 70-71 -9.343603131865752 1103 72-73 -7.078644961080219 1103 74-75 -5.682865805573595 1103 76-77 -2.823616720426955 1103 78-79 -0.6933861336318863 1103 80-81 -0.13117604268724392 1103 82-83 0.1099143558395852 1103 84-85 0.09340168209241284 1103 86-87 0.21987975460395504 1103 88-89 0.3019850787503451 1103 90-91 -0.009243450748140702 1103 92-93 0.04653726731504548 1103 94-95 0.6983213322580966 1103 96-97 0.8156061616108197 1103 98-99 0.564040746055035 1103 100-101 0.22700005186646166 1104 1 0.24440369486200453 1104 2 0.01793481295671384 1104 3 0.24523141375743407 1104 4 0.06418549684471486 1104 5 0.20892075578220215 1104 6 0.4189939609401634 1104 7 0.4320531677530184 1104 8 0.2144027524854195 1104 9 0.3424656452103392 1104 10-11 0.6529300549615087 1104 12-13 0.5407442025636229 1104 14-15 0.8306752808341429 1104 16-17 0.7685762523306892 1104 18-19 0.7213409964638657 1104 20-21 0.8485021515413607 1104 22-23 0.7967613467207215 1104 24-25 0.709260650066966 1104 26-27 0.9461576925833555 1104 28-29 0.9823408003967273 1104 30-31 1.0739962796545512 1104 32-33 0.8653640002300236 1104 34-35 0.7896712547475104 1104 36-37 1.3972799165705183 1104 38-39 1.3288020927288393 1104 40-41 1.4891970933068457 1104 42-43 1.3302150936800743 1104 44-45 1.2724489567882067 1104 46-47 1.158735606865072 1104 48-49 0.7860073344076532 1104 50-51 0.8134830161294566 1104 52-53 0.9587359373749749 1104 54-55 0.7422871695108135 1104 56-57 1.0327865243778263 1104 58-59 0.9618701611692018 1104 60-61 0.7812919908957277 1104 62-63 0.8297504485275589 1104 64-65 0.5847320561061125 1104 66-67 -2.2721283354380546 1104 68-69 -6.191412489674281 1104 70-71 -7.230028607209295 1104 72-73 -6.514121219217648 1104 74-75 -5.409026767464454 1104 76-77 -2.825775813934321 1104 78-79 -0.7913603094363211 1104 80-81 -0.3511292024438326 1104 82-83 -0.1691280649582687 1104 84-85 -0.22219997332148722 1104 86-87 0.01711114997938168 1104 88-89 -0.12556116537420792 1104 90-91 -0.23404313847985136 1104 92-93 -0.16636862429911048 1104 94-95 0.2446389509184108 1104 96-97 0.37781238933802186 1104 98-99 -0.019996497491366227 1104 100-101 -0.11858638210153316 1105 1 0.3055624689843661 1105 2 0.4363683351192087 1105 3 0.46092289199704695 1105 4 0.38203612557827427 1105 5 0.4266227659924837 1105 6 0.5186664854927798 1105 7 0.5843549855956098 1105 8 0.5127094020384391 1105 9 0.5949562922156133 1105 10-11 0.7135218590681447 1105 12-13 1.0760740745651134 1105 14-15 0.9840378553444538 1105 16-17 0.9594392691580396 1105 18-19 0.7096091403085794 1105 20-21 0.9303686007488849 1105 22-23 1.0104028865630355 1105 24-25 1.0444050705994812 1105 26-27 1.1610891897380498 1105 28-29 1.2003572578072266 1105 30-31 1.0347354155439916 1105 32-33 1.0662249904411496 1105 34-35 1.3011827565988057 1105 36-37 1.518464328996565 1105 38-39 1.503056507052836 1105 40-41 1.7329403178141085 1105 42-43 1.6217752021522926 1105 44-45 1.3133858003495185 1105 46-47 1.3173617540746143 1105 48-49 1.0272985141234763 1105 50-51 0.6745021789117764 1105 52-53 1.1929556740435423 1105 54-55 0.9136633062655903 1105 56-57 0.9719558690730814 1105 58-59 1.1407713169838196 1105 60-61 1.4758029169306717 1105 62-63 1.3035701054182134 1105 64-65 1.036868527799431 1105 66-67 1.4428280923130785 1105 68-69 -4.916431227832248 1105 70-71 -4.005471487191798 1105 72-73 -1.4580764342158936 1105 74-75 0.43576338125057745 1105 76-77 0.7733916649872583 1105 78-79 0.2789648820467434 1105 80-81 0.1775474171603051 1105 82-83 0.5220638748994837 1105 84-85 0.40236776230481297 1105 86-87 0.4885246022075336 1105 88-89 0.29907444581142784 1105 90-91 0.24580248483331246 1105 92-93 0.4847477838935639 1105 94-95 1.0021623615526813 1105 96-97 0.6677335121477519 1105 98-99 0.7321571843556178 1105 100-101 0.44280802636778915 1106 1 0.2193527573297267 1106 2 0.2588904667880598 1106 3 0.41239571898827165 1106 4 0.13339481489209248 1106 5 0.17754605272219237 1106 6 0.3834973082637134 1106 7 0.24698795832230047 1106 8 0.2404630512563415 1106 9 0.42055734215784213 1106 10-11 0.3647527266233439 1106 12-13 0.3940135940361671 1106 14-15 0.5265829078493454 1106 16-17 0.3952088354504113 1106 18-19 0.397833677421211 1106 20-21 0.4687781062093066 1106 22-23 0.5593055943006036 1106 24-25 0.7760944481759395 1106 26-27 0.8165862251129568 1106 28-29 0.9167018043121118 1106 30-31 0.6479214273029683 1106 32-33 0.4585468213712076 1106 34-35 0.6788105278196355 1106 36-37 0.6669319451518732 1106 38-39 0.9154076343567716 1106 40-41 0.6645043616467063 1106 42-43 0.5733514372572515 1106 44-45 0.3490266118777363 1106 46-47 -0.08756731417940244 1106 48-49 -0.24800109727971886 1106 50-51 -0.30637276258100243 1106 52-53 -0.1270621152620386 1106 54-55 -0.2276808126117551 1106 56-57 -0.16011883979031794 1106 58-59 -0.025019879486933405 1106 60-61 -0.2908679482609138 1106 62-63 -0.21210283260067442 1106 64-65 -0.08900013923110706 1106 66-67 0.45365873159292036 1106 68-69 -5.422833200827956 1106 70-71 -5.027724446763111 1106 72-73 -2.224958003158008 1106 74-75 -0.6098427069215582 1106 76-77 -0.4511337850712849 1106 78-79 -0.3815361730270155 1106 80-81 -0.34696030579303283 1106 82-83 0.07017974796718818 1106 84-85 -0.36453868678496093 1106 86-87 -0.141443069150597 1106 88-89 -0.34820747580677747 1106 90-91 -0.2517398230802854 1106 92-93 -0.0768500191894006 1106 94-95 0.6553222180564298 1106 96-97 0.7483770682672564 1106 98-99 0.2461157246995569 1106 100-101 -0.026286165206236944 1107 1 0.10703140568981695 1107 2 -0.03156726012423405 1107 3 0.025491291568364716 1107 4 0.05678440411793417 1107 5 0.3173990756178995 1107 6 0.07627363958032163 1107 7 0.1302616641558032 1107 8 0.0156517676105139 1107 9 0.10628295575915558 1107 10-11 -0.0804820683641978 1107 12-13 0.14541218670229483 1107 14-15 0.15012187801061572 1107 16-17 0.14813089512786348 1107 18-19 0.4178994237639557 1107 20-21 0.2856881217561593 1107 22-23 0.20284787363203094 1107 24-25 0.4051232067376418 1107 26-27 0.05479262367440185 1107 28-29 -0.34649117025722376 1107 30-31 0.1982067651817303 1107 32-33 0.4337046313249502 1107 34-35 0.6587603907700412 1107 36-37 0.7106590385141729 1107 38-39 0.6082869870081744 1107 40-41 0.20432444893387114 1107 42-43 0.34233389865546826 1107 44-45 0.23419060311155349 1107 46-47 0.31341747612535187 1107 48-49 0.8177897319483698 1107 50-51 0.619607969307534 1107 52-53 0.7244810194586471 1107 54-55 0.9296265218829127 1107 56-57 1.104173809481349 1107 58-59 0.8503274272856274 1107 60-61 1.0383265809940205 1107 62-63 0.9922079858882213 1107 64-65 0.6596761927098775 1107 66-67 1.3654506160092268 1107 68-69 -4.279859099767151 1107 70-71 -3.4478587179027755 1107 72-73 -0.6715671964073024 1107 74-75 0.9248458252429508 1107 76-77 0.660742701713886 1107 78-79 0.7645418359764236 1107 80-81 0.6926186179149845 1107 82-83 0.55345204896431 1107 84-85 0.6339081885467373 1107 86-87 0.6207345208994148 1107 88-89 0.30915293028459345 1107 90-91 0.19657000265756608 1107 92-93 0.08377507056403743 1107 94-95 0.5922029193507505 1107 96-97 0.6133847792580696 1107 98-99 0.5213504884685172 1107 100-101 0.35695320018234256 1108 1 0.20963670634175813 1108 2 -0.046635322370192966 1108 3 0.10079459568664006 1108 4 0.01692774499660743 1108 5 0.24281741017452418 1108 6 0.11501237554075061 1108 7 0.1543903008754981 1108 8 0.0031125246526571004 1108 9 0.4407994886862383 1108 10-11 0.362611787838496 1108 12-13 0.18120577336986798 1108 14-15 0.17197843227846477 1108 16-17 0.02057817541356144 1108 18-19 0.08698954224838928 1108 20-21 0.05967721082610211 1108 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0.20403637122483076 2316 96-97 0.6074415034413576 2316 98-99 0.03463151772696804 2316 100-101 -0.17009844785881256 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9.0 3 32.0 4 85.0 5 310.0 6 747.0 7 1516.0 8 2519.0 9 4128.0 10 6410.0 11 8929.0 12 10362.0 13 12320.0 14 14856.0 15 18646.0 16 21686.0 17 23486.0 18 18410.0 19 13466.0 20 12557.0 21 12830.0 22 13477.0 23 14747.0 24 16075.0 25 17816.0 26 20499.0 27 24176.0 28 28207.0 29 31611.0 30 35694.0 31 40477.0 32 46295.0 33 52776.0 34 63471.0 35 69418.0 36 43632.0 37 27568.0 38 9515.0 39 464.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.82051060006603 21.297418855506784 16.104956078868604 11.77711446555858 2 15.73441286343827 27.557784130961576 37.40506868609514 19.30273431950501 3 21.292627529175906 36.101961113222934 31.279735614286892 11.325675743314264 4 10.82000965879038 22.293137524130067 34.77245702582306 32.11439579125649 5 9.111200694784518 45.846985073496185 28.78837480486241 16.253439426856886 6 21.28762227365761 39.593735584525916 17.67098696874962 21.447655173066853 7 20.794266412165204 45.59652499989854 17.64961317491474 15.95959541302151 8 27.158651719440545 31.414742236104665 18.364147219445282 23.0624588250095 9 29.76598401294332 19.804172759776144 19.83298679830038 30.596856428980157 10-11 23.64841191359035 31.977698204736594 21.204629726077247 23.169260155595808 12-13 30.40083980070966 32.09836544588034 24.14379693272531 13.35699782068469 14-15 25.973285463249923 30.171747902865576 31.20465678151248 12.650309852372018 16-17 14.49839019507048 34.85707908335813 31.81238332296204 18.832147398609344 18-19 13.682524522701847 30.063121184286533 41.745477961020704 14.508876331990914 20-21 17.0292320449986 27.64808454282402 41.28145363442425 14.041229777753127 22-23 15.514262949069495 29.13356321434804 40.79668787361865 14.555485962963816 24-25 15.069742148174504 29.801020801571383 42.36319757366857 12.76603947658555 26-27 15.519893292786035 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14.457425928922365 52-53 13.201765578263434 35.39495709585254 35.97382633787363 15.429450988010393 54-55 12.03343429045194 34.66946413766265 36.266865196866384 17.030236375019033 56-57 14.132808096691537 35.9756877322917 33.09633631203495 16.79516785898181 58-59 13.636557357641765 34.38468433425223 34.21103012058381 17.7677281875222 60-61 14.348653120531202 33.57261622424586 34.85151247441328 17.227218180809658 62-63 17.021044892117278 33.302241392955004 34.14848780210645 15.528225912821267 64-65 15.981252401283571 33.98851171826382 35.41913559202801 14.611100288424595 66-67 15.092502368155692 33.68423911751824 35.373353749834486 15.849904764491585 68-69 15.988696059366136 34.579602330858386 34.04030675503153 15.391394854743954 70-71 15.755501542730881 34.11901300696429 33.210155896656474 16.915329553648355 72-73 14.749409361785231 33.50966585202445 33.26968093330331 18.471243852887007 74-75 14.269438867710848 34.134554163855725 34.663214962082726 16.932792006350702 76-77 14.505842114093051 32.98785305689499 35.39890896692806 17.107395862083898 78-79 13.197833224877328 32.80731043085219 36.78877324757868 17.2060830966918 80-81 12.836524348257806 34.70558473832235 35.352761254905566 17.105129658514283 82-83 12.559809421514212 34.11000469411801 34.27443410175279 19.055751782614987 84-85 13.191418557052472 32.60693998969188 34.028770749827856 20.17287070342779 86-87 14.020213143007107 32.68023678948949 34.51290681283836 18.78664325466504 88-89 14.845833601071403 31.268672616652065 35.040904740485715 18.84458904179081 90-91 13.26656714418967 31.848365082897445 35.883827913146234 19.001239859766653 92-93 12.756708129569883 33.445817522642265 34.84764243149828 18.94983191628958 94-95 13.10704090180801 33.467734283289815 33.83799771728492 19.587227097617248 96-97 12.846510997390451 32.330904531128205 33.1275290609008 21.69505541058054 98-99 13.9539210035691 31.794006943373354 33.02817396857352 21.223898084484023 100-101 14.706049471570893 31.649129025450094 33.59157273273338 20.053248770245627 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 21688.0 1 14233.5 2 6901.5 3 7036.0 4 6295.0 5 6370.5 6 10297.0 7 18664.5 8 25318.5 9 26944.0 10 22977.5 11 16543.5 12 13164.5 13 11008.0 14 9277.0 15 7654.0 16 6193.0 17 5371.0 18 4873.5 19 4570.0 20 4328.0 21 4274.5 22 4409.5 23 4491.0 24 4635.0 25 4928.5 26 5528.5 27 6084.0 28 6549.5 29 7116.0 30 7936.0 31 8834.0 32 9765.5 33 10981.0 34 12295.0 35 13272.0 36 14262.5 37 15105.5 38 14703.0 39 13737.0 40 13071.5 41 12639.0 42 12133.0 43 11944.0 44 12427.0 45 13068.5 46 15867.0 47 24815.5 48 46047.0 49 45247.0 50 25071.0 51 15971.5 52 9482.0 53 6908.5 54 5983.0 55 5778.0 56 5981.5 57 5993.0 58 5763.0 59 5845.5 60 6239.5 61 6170.5 62 5172.0 63 3456.5 64 2297.0 65 1873.5 66 1467.0 67 1115.0 68 804.5 69 530.0 70 397.0 71 325.5 72 299.0 73 338.5 74 293.5 75 197.0 76 139.0 77 92.5 78 71.0 79 48.5 80 28.5 81 20.0 82 17.0 83 10.5 84 4.0 85 4.5 86 2.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02313239712508945 2 0.01839769595913547 3 0.0 4 0.0 5 1.3527717617011376E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 4.058315285103413E-4 18-19 0.0013527717617011375 20-21 0.0 22-23 0.0 24-25 0.0 26-27 5.41108704680455E-4 28-29 0.019006443251900984 30-31 0.004802339754039038 32-33 0.008184269158291882 34-35 0.009875233860418305 36-37 0.09624971084503593 38-39 0.09821122989950258 40-41 0.11255061057353463 42-43 0.09733192825439684 44-45 0.09334125155737849 46-47 0.09381472167397388 48-49 0.0953704091999302 50-51 0.09367944449780377 52-53 0.0732525908961166 54-55 0.062295139626337384 56-57 0.03442804133529395 58-59 0.014948127966797571 60-61 0.009469402331907963 62-63 0.0023673505829769907 64-65 0.0047347011659539815 66-67 0.39101867771971377 68-69 0.7674950590011405 70-71 0.8481202559985282 72-73 0.25499747708066445 74-75 0.14028243168840798 76-77 0.014474657850202171 78-79 0.008116630570206824 80-81 0.0016233261140413651 82-83 0.0 84-85 0.0 86-87 1.3527717617011376E-4 88-89 7.440244689356256E-4 90-91 0.0035848451685080145 92-93 0.00108221740936091 94-95 0.2035921501360212 96-97 0.20940906871133608 98-99 0.013662994793181489 100-101 0.006899135984675802 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 739223.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 27.667189622950893 #Duplication Level Percentage of deduplicated Percentage of total 1 75.38589624712819 20.857158863653975 2 10.239524993860588 5.665977593081719 3 4.3652078745580445 3.6231910202698754 4 2.4323991240259595 2.691905912125035 5 1.5553131630136499 2.151557210208509 6 1.0386092532791986 1.7241239492776215 7 0.7984164913183149 1.5462958324376461 8 0.5614149662799572 1.242621946338413 9 0.45640404035114723 1.136467541616851 >10 2.803422758432695 14.39434416485119 >50 0.18514234281147027 3.5653590673173174 >100 0.13836435726060986 7.5116261607751325 >500 0.01920359406819852 3.497923633502526 >1k 0.01575679513288084 9.441778133024073 >5k 0.0024619992395126305 5.02496156806904 >10k+ 0.0024619992395126305 15.924707403451116 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 30607 4.140428531038672 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 27806 3.761517160586183 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 27525 3.723504274082381 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20867 2.822828835141764 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10088 1.3646761532041074 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 9520 1.287838717139483 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 7931 1.0728832842051723 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7556 1.0221543431413795 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 5991 0.8104455624351515 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5887 0.7963767361134596 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4691 0.6345852334140036 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 4410 0.5965723469102017 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3950 0.5343448458719493 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 3886 0.525687106597062 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 3812 0.5156765955604736 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3723 0.5036369268813335 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 3418 0.4623773881494488 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2649 0.35834923967463134 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 2592 0.35063844063293487 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 2376 0.32141857058019024 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 2175 0.2942278581699974 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 2131 0.2882756624185124 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2081 0.2815118036100067 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 1909 0.25824412930874713 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1901 0.25716191189938625 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 1812 0.24512224322024612 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1658 0.2242895580900486 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 1653 0.22361317220919805 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1650 0.2232073406806877 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1545 0.20900323718282574 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1532 0.20724463389261427 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 1494 0.20210410119814995 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1490 0.20156299249346948 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 1414 0.19128192710454084 No Hit GTACATGGGACTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAAA 1387 0.18762944334794776 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1363 0.18438279111986505 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 1234 0.16693203539392037 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 1191 0.1611151168186055 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1088 0.14718156767308377 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 1054 0.14258214368329988 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 1035 0.14001187733606774 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1002 0.13554773052245397 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 932 0.126078328190546 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 876 0.11850280632501964 No Hit CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 863 0.11674420303480817 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 838 0.11336227363055533 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 832 0.11255061057353463 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 825 0.11160367034034385 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 813 0.1099803442263025 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 810 0.10957451269779214 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 808 0.10930395834545191 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 780 0.10551619741268872 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 4.0583152851034123E-4 0.0 12-13 0.0 0.0 0.0 0.0010145788212758532 0.0 14-15 0.0 0.0 0.0 0.0018938804663815925 0.0 16-17 0.0 0.0 0.0 0.0035848451685080145 0.0 18-19 0.0 0.0 0.0 0.00541108704680455 0.0 20-21 0.0 0.0 0.0 0.008725377862972337 0.0 22-23 0.0 0.0 0.0 0.018059503018710187 0.0 24-25 0.0 0.0 0.0 0.032872353809337646 0.0 26-27 0.0 0.0 0.0 0.04782048177613521 0.0 28-29 0.0 0.0 0.0 0.071358710429735 0.0 30-31 0.0 0.0 0.0 0.12614596677863107 0.0 32-33 0.0 0.0 0.0 0.19216122874964658 0.0 34-35 0.0 0.0 0.0 0.2717718469257585 0.0 36-37 0.0 0.0 0.0 0.3822932998567415 0.0 38-39 0.0 0.0 0.0 0.5258900223613172 0.0 40-41 0.0 0.0 0.0 0.6928896963433226 0.0 42-43 0.0 0.0 0.0 0.8342543454410916 0.0 44-45 0.0 0.0 0.0 0.9711548477252467 0.0 46-47 0.0 0.0 0.0 1.1388985461761876 0.0 48-49 0.0 0.0 0.0 1.3278807612858365 0.0 50-51 0.0 0.0 0.0 1.5063113566542166 0.0 52-53 0.0 0.0 0.0 1.6713495115817554 6.763858808505688E-5 54-55 0.0 0.0 0.0 1.8247538293586643 1.3527717617011376E-4 56-57 0.0 0.0 0.0 1.9770759297262126 1.3527717617011376E-4 58-59 0.0 0.0 0.0 2.1425199161822617 1.3527717617011376E-4 60-61 0.0 0.0 0.0 2.3516584305412573 1.3527717617011376E-4 62-63 0.0 0.0 0.0 2.529412640028787 1.3527717617011376E-4 64-65 0.0 0.0 0.0 2.679570305577613 1.3527717617011376E-4 66-67 0.0 0.0 0.0 2.8309454657119706 1.3527717617011376E-4 68-69 0.0 0.0 0.0 3.0149224253033253 1.3527717617011376E-4 70-71 0.0 0.0 0.0 3.2659968642750563 1.3527717617011376E-4 72-73 0.0 0.0 0.0 3.564891785022923 1.3527717617011376E-4 74-75 0.0 0.0 0.0 3.8426158277001665 1.3527717617011376E-4 76-77 0.0 0.0 0.0 4.09010542150339 1.3527717617011376E-4 78-79 0.0 0.0 0.0 4.385280219906577 1.3527717617011376E-4 80-81 0.0 0.0 0.0 4.711568768828892 1.3527717617011376E-4 82-83 0.0 0.0 0.0 5.049964625018431 1.3527717617011376E-4 84-85 0.0 0.0 0.0 5.474734958192588 1.3527717617011376E-4 86-87 0.0 0.0 0.0 5.890644636327603 1.3527717617011376E-4 88-89 0.0 0.0 0.0 6.231881313216716 1.3527717617011376E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 40 7.648023E-6 59.236427 2 TAGCCGA 15 0.00977135 48.168045 64-65 GCGTATC 40 5.4420694E-4 47.40197 1 ATAGTCA 70 4.38598E-6 40.608273 8 CGTTTAG 30 7.636758E-5 40.140038 64-65 CGTCACT 125 1.382432E-10 37.921574 1 ATAACGC 65 1.3482475E-4 36.443325 3 GTGTTGT 385 0.0 33.738422 68-69 GTCACTA 205 0.0 32.372078 1 GGGAATG 205 0.0 32.354557 7 GTCAAGC 30 0.0039743134 31.629143 38-39 CTTAACG 30 0.0040020645 31.584213 20-21 GATAGTC 90 2.4551682E-5 31.584211 7 TAGATAG 110 2.7877904E-6 30.148567 5 TAGGATG 65 0.005917649 29.15466 5 TGTAGTA 100 5.0302297E-5 28.433483 2 GATATAG 135 4.2986358E-7 28.090055 1 AGGCTTA 135 4.3190266E-7 28.074858 6 CTAGATA 170 3.739842E-9 27.868422 4 CATGGGT 545 0.0 27.817287 4 >>END_MODULE