##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939500_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 735625 Sequences flagged as poor quality 0 Sequence length 101 %GC 32 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.79485471537808 31.0 30.0 31.0 28.0 34.0 2 30.86833372982158 31.0 31.0 33.0 28.0 34.0 3 30.75867323704333 31.0 30.0 33.0 27.0 34.0 4 34.720017672047575 35.0 35.0 37.0 32.0 37.0 5 34.37827153780799 35.0 35.0 37.0 30.0 37.0 6 34.56514392523364 35.0 35.0 37.0 30.0 37.0 7 34.37650297366185 35.0 35.0 37.0 30.0 37.0 8 34.36781512319456 35.0 35.0 37.0 30.0 37.0 9 35.61922446898895 37.0 35.0 39.0 30.0 39.0 10-11 35.44106032285471 37.0 34.5 39.0 30.0 39.0 12-13 35.38098079864061 37.0 34.0 39.0 30.0 39.0 14-15 35.614041121495326 38.0 33.5 40.0 27.0 41.0 16-17 35.69519388275276 38.0 33.5 40.0 28.5 41.0 18-19 36.12925811384876 38.0 34.0 40.0 30.0 41.0 20-21 36.23454749362787 38.0 34.5 40.0 30.0 41.0 22-23 36.023946983857265 38.0 34.0 40.0 29.5 41.0 24-25 35.761825658453695 38.0 34.0 40.0 29.0 41.0 26-27 35.05880509770603 37.0 33.5 40.0 26.5 41.0 28-29 34.68106576040782 37.0 33.0 39.5 26.0 41.0 30-31 34.02593033135089 35.5 33.0 39.0 25.0 40.0 32-33 33.05507425658454 35.0 32.5 38.0 23.5 40.0 34-35 32.595214953271025 35.0 31.0 38.0 21.5 40.0 36-37 31.977642141036533 35.0 31.0 38.0 19.0 40.0 38-39 31.459471877655055 35.0 31.0 38.0 17.0 40.0 40-41 30.994669158878505 35.0 30.0 38.0 15.0 40.0 42-43 30.520534239592184 35.0 30.0 38.0 10.0 40.0 44-45 29.72279422259983 34.5 27.0 37.5 2.0 40.0 46-47 28.741615632965164 33.5 24.0 37.0 2.0 40.0 48-49 27.9836948173322 33.0 23.0 37.0 2.0 40.0 50-51 27.62235649957519 33.0 23.0 37.0 2.0 40.0 52-53 27.36815225148683 33.0 23.0 36.0 2.0 40.0 54-55 27.082096176720476 33.0 22.0 36.0 2.0 39.5 56-57 26.642677315208157 33.0 20.5 35.5 2.0 39.0 58-59 26.26444927782498 33.0 20.0 35.0 2.0 39.0 60-61 25.880424129141886 33.0 19.0 35.0 2.0 39.0 62-63 25.57754358538658 33.0 17.5 35.0 2.0 38.5 64-65 25.011325743415462 32.0 12.0 35.0 2.0 38.0 66-67 24.26236465590484 31.5 6.5 35.0 2.0 37.5 68-69 21.547547323704332 27.0 2.0 34.0 2.0 36.0 70-71 20.499739677145286 26.0 2.0 33.0 2.0 35.0 72-73 21.184611724723872 26.5 2.0 33.0 2.0 35.0 74-75 21.661094307561598 28.5 2.0 33.0 2.0 35.0 76-77 21.151385556499577 28.0 2.0 33.0 2.0 35.0 78-79 21.96008700084962 29.0 2.0 34.0 2.0 35.0 80-81 21.928997111299914 29.0 2.0 34.0 2.0 35.0 82-83 21.622913169073918 29.0 2.0 34.0 2.0 35.0 84-85 21.270022090059474 29.0 2.0 34.0 2.0 35.0 86-87 20.77390790144435 29.0 2.0 34.0 2.0 35.0 88-89 20.176456754460492 28.0 2.0 33.0 2.0 35.0 90-91 19.5111367884452 26.5 2.0 33.0 2.0 35.0 92-93 19.05520271877655 25.5 2.0 33.0 2.0 35.0 94-95 18.345990144435007 24.5 2.0 33.0 2.0 35.0 96-97 17.688262361937127 23.5 2.0 33.0 2.0 35.0 98-99 16.987938147833475 19.0 2.0 33.0 2.0 35.0 100-101 16.1273264231096 8.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.19269515738314524 1101 2 0.2027498207685099 1101 3 0.25877732508482154 1101 4 0.28058237611956827 1101 5 0.4292620596589387 1101 6 0.5065840247953091 1101 7 0.5380289340650037 1101 8 0.5864019931401714 1101 9 0.5769739499207773 1101 10-11 0.7944332321968304 1101 12-13 0.5540970498992976 1101 14-15 0.6732316117917563 1101 16-17 0.6905811653335405 1101 18-19 0.414902470706096 1101 20-21 0.5431902700087576 1101 22-23 0.5191819918438583 1101 24-25 0.535815243251129 1101 26-27 0.7249529136983313 1101 28-29 0.6330157181489326 1101 30-31 0.37357643202740576 1101 32-33 0.731405139501021 1101 34-35 0.8870517824765258 1101 36-37 1.0465635624969991 1101 38-39 1.233461149735902 1101 40-41 1.2612970609105396 1101 42-43 0.9758815536415497 1101 44-45 0.8925532127027402 1101 46-47 0.5013214271222282 1101 48-49 0.40256293021537104 1101 50-51 0.5075680538405187 1101 52-53 0.23660335384066045 1101 54-55 0.4438131863567847 1101 56-57 0.3558951685027232 1101 58-59 0.47375513443993 1101 60-61 0.3451967225518118 1101 62-63 -0.05944686200559701 1101 64-65 -0.3466971346667691 1101 66-67 -0.1284853886728321 1101 68-69 -5.586271235604716 1101 70-71 -6.54570766318608 1101 72-73 -3.9818155828495314 1101 74-75 -2.1873620630218333 1101 76-77 -0.28220048839440537 1101 78-79 -0.11550657961052124 1101 80-81 -0.16980366770963684 1101 82-83 -0.13029601602239893 1101 84-85 -0.5732845625525371 1101 86-87 -0.4336179012881516 1101 88-89 -0.4588166689436761 1101 90-91 -0.5844994826507417 1101 92-93 -0.7752720053456486 1101 94-95 -0.9349125663300093 1101 96-97 -0.8488207729308606 1101 98-99 -0.8170590312165267 1101 100-101 -0.7588937488598084 1102 1 0.3717684685180842 1102 2 0.3909376417516377 1102 3 0.3158065028036532 1102 4 0.43048855028289523 1102 5 0.43378051442500976 1102 6 0.42290619308923283 1102 7 0.44940937314170526 1102 8 0.5826736506829207 1102 9 0.9028070951321325 1102 10-11 0.8097945674866338 1102 12-13 0.7386588197508672 1102 14-15 0.7501441068535613 1102 16-17 0.9993545865198428 1102 18-19 0.5374968609087034 1102 20-21 0.6360143571468626 1102 22-23 0.5058753012856982 1102 24-25 0.2817200629362162 1102 26-27 0.7302508989119474 1102 28-29 0.7092121739308581 1102 30-31 0.740550090247396 1102 32-33 0.7121708429831628 1102 34-35 0.7976227091653882 1102 36-37 1.0465406351308957 1102 38-39 1.1136198071093446 1102 40-41 1.2169731713005127 1102 42-43 0.8857911140004866 1102 44-45 0.9957492875376666 1102 46-47 1.2347713960821025 1102 48-49 1.025622922314252 1102 50-51 0.7253780318302496 1102 52-53 0.8901637974322867 1102 54-55 0.7368213978811369 1102 56-57 0.6722381477904911 1102 58-59 0.7065649007924328 1102 60-61 0.5787119463229082 1102 62-63 0.5657788843683527 1102 64-65 0.6164039150538692 1102 66-67 0.7637542269939672 1102 68-69 -5.0378750930458835 1102 70-71 -5.750009895253353 1102 72-73 -0.29682172032907417 1102 74-75 0.9194406628656324 1102 76-77 1.1869657314825623 1102 78-79 1.0242410077251094 1102 80-81 1.0033649648317038 1102 82-83 0.7664585271233761 1102 84-85 0.6034254662301244 1102 86-87 0.5085786814761128 1102 88-89 0.21049134787442725 1102 90-91 0.03584626203010899 1102 92-93 0.2612407069970324 1102 94-95 0.49577095136286076 1102 96-97 0.47328932075388863 1102 98-99 0.2913380284369502 1102 100-101 0.37619429222603173 1103 1 0.3276857868617036 1103 2 0.368470248505254 1103 3 0.4504871696444077 1103 4 0.30686806678211553 1103 5 0.7208024080218536 1103 6 0.3667772228991595 1103 7 0.3577290773788633 1103 8 0.3919567831302473 1103 9 0.69845311429075 1103 10-11 0.5603975690946399 1103 12-13 0.563288429019579 1103 14-15 0.4250712656336688 1103 16-17 0.4472542168162903 1103 18-19 0.6964590796797481 1103 20-21 0.6311353697772546 1103 22-23 0.6094249363924149 1103 24-25 0.8481498811080357 1103 26-27 0.5746249454478658 1103 28-29 0.581877475397313 1103 30-31 0.564614492880672 1103 32-33 0.8330546268783792 1103 34-35 1.182653838479169 1103 36-37 1.377298183817686 1103 38-39 1.254699849989457 1103 40-41 1.26815958984891 1103 42-43 0.8220189695824622 1103 44-45 1.2132765342769893 1103 46-47 1.2230996213676235 1103 48-49 1.017682542165545 1103 50-51 1.2521111031184375 1103 52-53 0.9190460282977249 1103 54-55 0.7013027134210539 1103 56-57 0.9235455102511487 1103 58-59 1.0150577747221483 1103 60-61 1.171886861456013 1103 62-63 0.8689237696777887 1103 64-65 0.48786493125760444 1103 66-67 0.734839355015847 1103 68-69 -5.048179376934229 1103 70-71 -6.041742416796488 1103 72-73 -0.1484558375072318 1103 74-75 1.0999339903350176 1103 76-77 1.0261068922692473 1103 78-79 0.963116229341086 1103 80-81 0.8032423029661437 1103 82-83 0.9545864390542178 1103 84-85 0.8197123506874568 1103 86-87 0.4610222982488352 1103 88-89 0.5989326144870368 1103 90-91 0.7521675055534267 1103 92-93 0.9330257291929911 1103 94-95 1.1082917385977815 1103 96-97 0.9280057897413947 1103 98-99 0.9760935980415475 1103 100-101 0.9613961860977476 1104 1 0.2655902645940067 1104 2 0.22089425877386404 1104 3 0.13815475699143676 1104 4 0.12140977629066185 1104 5 0.31754440919118565 1104 6 0.34071592037165743 1104 7 0.5778708083923476 1104 8 0.42400754823579234 1104 9 0.2785859073123973 1104 10-11 0.45141539131596176 1104 12-13 0.42636158614678266 1104 14-15 0.5528349867952116 1104 16-17 0.43830362442231774 1104 18-19 0.5567878364924468 1104 20-21 0.3643951158845269 1104 22-23 0.5907567979812285 1104 24-25 0.3573667651762378 1104 26-27 0.42430371536514144 1104 28-29 0.4336580174562741 1104 30-31 0.5854860478896455 1104 32-33 0.6953801512293722 1104 34-35 0.9028358912603593 1104 36-37 1.1306566510528953 1104 38-39 1.0059338640886395 1104 40-41 1.0505421695012096 1104 42-43 0.7329271837985729 1104 44-45 0.7585629196321229 1104 46-47 0.8910129026858797 1104 48-49 0.7310048542157048 1104 50-51 0.6935899375505379 1104 52-53 0.43109551654729117 1104 54-55 0.4889724848638366 1104 56-57 0.6149285804849498 1104 58-59 0.4103522244308273 1104 60-61 0.4773880946300757 1104 62-63 0.3507243718161881 1104 64-65 0.427547766953122 1104 66-67 0.3301558136893945 1104 68-69 -6.0319112237383905 1104 70-71 -3.495016208319509 1104 72-73 0.298484076338589 1104 74-75 0.42843291172773235 1104 76-77 0.5976797100215769 1104 78-79 0.0673883206295649 1104 80-81 0.05510802475938803 1104 82-83 -0.025947387548740153 1104 84-85 0.0940616581610989 1104 86-87 -0.35780191900484937 1104 88-89 -0.2366982195687264 1104 90-91 0.03130670301584004 1104 92-93 0.10415803163312987 1104 94-95 0.2158475338438457 1104 96-97 -0.13089003225144324 1104 98-99 -0.014921368054565676 1104 100-101 -0.11312768102858684 1105 1 0.3310199280747028 1105 2 0.25954568918177756 1105 3 0.3637077928471477 1105 4 0.2838320331655879 1105 5 0.6213415039390071 1105 6 0.3159201062435173 1105 7 0.20999681743375476 1105 8 0.4431430561630094 1105 9 0.5424919449176855 1105 10-11 0.3985517059843886 1105 12-13 0.48597025107206804 1105 14-15 0.611404070570039 1105 16-17 0.28431771615797174 1105 18-19 0.23128087670981046 1105 20-21 0.38964103257701765 1105 22-23 0.48359631387091895 1105 24-25 0.3525517335542716 1105 26-27 0.7131277260823339 1105 28-29 0.46717057195639455 1105 30-31 0.48528780599517773 1105 32-33 1.1071297138346168 1105 34-35 0.6473227497412282 1105 36-37 0.6870982008486664 1105 38-39 0.5848805421021552 1105 40-41 0.3577646349482535 1105 42-43 0.09396027166613763 1105 44-45 0.47256824215643434 1105 46-47 0.45032887796549304 1105 48-49 0.6163443004173779 1105 50-51 0.4370372148809487 1105 52-53 0.15417503794642684 1105 54-55 0.2960252395079266 1105 56-57 0.37668002876578655 1105 58-59 0.22091315552823687 1105 60-61 0.46103026883042375 1105 62-63 0.010646878748094224 1105 64-65 0.020211505083612025 1105 66-67 0.057480564739226025 1105 68-69 -2.439218790544601 1105 70-71 -2.619482275177365 1105 72-73 0.3518946075907934 1105 74-75 0.7899710327093992 1105 76-77 0.6354998034527064 1105 78-79 0.32913157032088947 1105 80-81 0.3138858446232611 1105 82-83 -0.07153236510919925 1105 84-85 0.022060005325453602 1105 86-87 -0.23941264669525708 1105 88-89 -0.3186955927406494 1105 90-91 -0.1392485546576303 1105 92-93 -0.2685129540504896 1105 94-95 -0.1136865731083958 1105 96-97 -0.21830913266940755 1105 98-99 -0.08979990595090825 1105 100-101 0.07226866338432103 1106 1 0.024492145431807444 1106 2 0.18860115256550003 1106 3 0.19958337042487173 1106 4 0.1062931591197227 1106 5 0.2196212696916362 1106 6 0.11999109377006789 1106 7 0.3639708387758702 1106 8 0.28786978348263403 1106 9 0.4803014326368995 1106 10-11 0.3123568883413199 1106 12-13 0.4134812612718264 1106 14-15 0.537630159409531 1106 16-17 0.4810324890915112 1106 18-19 0.30238304308404196 1106 20-21 0.3407678465133941 1106 22-23 0.48681657837046544 1106 24-25 0.7520078312783554 1106 26-27 0.9143588304141304 1106 28-29 0.9673918905054393 1106 30-31 0.6930486036335992 1106 32-33 0.9983373170024947 1106 34-35 0.9342558796950229 1106 36-37 0.93968316965708 1106 38-39 0.9770785059715053 1106 40-41 0.7684450859922727 1106 42-43 0.9091409726886575 1106 44-45 1.1305968511613962 1106 46-47 1.2537733270096822 1106 48-49 1.0178424200058238 1106 50-51 0.5809977660437475 1106 52-53 0.6736009153391329 1106 54-55 0.742291802574158 1106 56-57 0.5693394179265923 1106 58-59 0.7650773516005493 1106 60-61 0.858395781986939 1106 62-63 0.43529428167841644 1106 64-65 0.7065044034649652 1106 66-67 0.6224240250149329 1106 68-69 -1.913343626944016 1106 70-71 -2.4114778679130247 1106 72-73 0.7350519475647452 1106 74-75 1.1144192489455804 1106 76-77 1.167771710008509 1106 78-79 0.6190819567526269 1106 80-81 0.4844181555391991 1106 82-83 0.3290382864589745 1106 84-85 0.34614439934252417 1106 86-87 0.37021168497049217 1106 88-89 0.5289958478042713 1106 90-91 0.6561233401157516 1106 92-93 0.5019852833622132 1106 94-95 0.6997761227744412 1106 96-97 0.6118734630946498 1106 98-99 0.684022886356761 1106 100-101 0.36073350876385746 1107 1 0.20074278168035775 1107 2 0.13271895316846738 1107 3 0.07354416446305834 1107 4 -0.0856189798500111 1107 5 0.19748634584568947 1107 6 0.07273577212195903 1107 7 0.28130420691254443 1107 8 0.2468790629229929 1107 9 0.15959301066380505 1107 10-11 -0.04193403712297794 1107 12-13 0.23751122694703497 1107 14-15 0.05266193920880369 1107 16-17 0.14738375049503816 1107 18-19 0.31303939056058283 1107 20-21 0.4232338153731092 1107 22-23 0.24219695711781242 1107 24-25 0.021077342247934894 1107 26-27 0.6011793495929396 1107 28-29 0.4348567500087057 1107 30-31 0.3249771871811902 1107 32-33 0.4321478911270873 1107 34-35 0.4287678281164702 1107 36-37 0.36758315791095697 1107 38-39 0.31299000542987443 1107 40-41 0.5576673618696404 1107 42-43 0.18795161206950795 1107 44-45 0.367421611997198 1107 46-47 0.5411048027147771 1107 48-49 0.8570607805075134 1107 50-51 0.7870851963826873 1107 52-53 0.3750045775205244 1107 54-55 0.18964468233297183 1107 56-57 0.520695769318511 1107 58-59 0.6303427488154121 1107 60-61 0.4659283122332134 1107 62-63 0.3268253240819341 1107 64-65 -0.0892076076024999 1107 66-67 0.05090153118164764 1107 68-69 -1.434837408022311 1107 70-71 -1.6589703960711013 1107 72-73 1.0723990847725595 1107 74-75 1.0228614514749168 1107 76-77 0.6375058559998159 1107 78-79 0.5738675246778726 1107 80-81 0.27539256203349893 1107 82-83 0.2881356618521451 1107 84-85 0.27890924187916966 1107 86-87 0.7239756661244812 1107 88-89 0.6267823554196781 1107 90-91 0.4995768319782954 1107 92-93 0.502326247327705 1107 94-95 0.4994834484423407 1107 96-97 0.6080576032128953 1107 98-99 0.4819226135855921 1107 100-101 0.8823806420562974 1108 1 0.014871916793946838 1108 2 0.0431480749965516 1108 3 -0.11045344414594638 1108 4 -0.11504637456237532 1108 5 -0.14035741660946854 1108 6 -0.19636878480643105 1108 7 0.1374288249542559 1108 8 0.13466304060334267 1108 9 0.2378645663965031 1108 10-11 0.3356566364521498 1108 12-13 0.1768330018905715 1108 14-15 0.29167814752552346 1108 16-17 0.3199558243624523 1108 18-19 0.031705525807019797 1108 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1.1812569146391318 2316 98-99 1.4606390370925446 2316 100-101 1.6923240562913655 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 29.0 4 108.0 5 365.0 6 845.0 7 1649.0 8 2640.0 9 4488.0 10 7030.0 11 9351.0 12 10872.0 13 12878.0 14 15638.0 15 19534.0 16 22333.0 17 23766.0 18 18891.0 19 14165.0 20 13394.0 21 13720.0 22 14363.0 23 15584.0 24 17142.0 25 18610.0 26 21374.0 27 24604.0 28 28727.0 29 32856.0 30 36604.0 31 40862.0 32 46205.0 33 52897.0 34 61341.0 35 62643.0 36 38284.0 37 23559.0 38 7868.0 39 401.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.6026635485428 21.418927646382997 16.20699266470424 11.77141614036996 2 15.737077252320233 27.779454673508404 37.250471117057295 19.23299695711407 3 21.35166878731526 36.14563962024078 31.248708579382946 11.25398301306102 4 10.764363280080559 22.3366355496285 34.807446316305146 32.09155485398579 5 9.127612574341546 46.07714528462192 28.651690739167375 16.143551401869157 6 21.240713678844518 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13.08735101580596 35.513017032788426 36.0131333230871 15.386498628318524 54-55 12.054870718027667 34.71137996877431 36.301768549093424 16.9319807641046 56-57 14.207033932298572 35.94654864128033 33.08926009776527 16.75715732865583 58-59 13.736354507796891 34.407953731949064 34.21032057091305 17.645371189341 60-61 14.332930445410952 33.6058151818502 34.852954972176256 17.208299400562595 62-63 16.87849752008706 33.43522149289604 34.10928745579352 15.576993531223385 64-65 15.956496618291812 34.064235462053496 35.30183869761751 14.67742922203718 66-67 14.778369385718545 33.913724774788875 35.4419746968482 15.865931142644374 68-69 15.99953461473971 34.58736410579302 33.958272736294234 15.454828543173038 70-71 15.870083759441785 34.10983316654557 33.043031241860525 16.97705183215211 72-73 14.983355464665912 33.42233607298088 33.16283000067519 18.43147846167801 74-75 14.361899995035591 34.18703037878443 34.37404579633545 17.07702382984453 76-77 14.218358024792241 33.12462036711691 35.41419820173181 17.242823406359037 78-79 13.335667283010661 32.880615075048944 36.576843593648036 17.206874048292363 80-81 12.912954553333103 34.71955149446335 35.26964777660639 17.09784617559715 82-83 12.578285049339803 34.19121195519927 34.20317476716698 19.027328228293953 84-85 13.232303459375986 32.647521016171304 33.99080236642632 20.129373158026382 86-87 14.030484800081563 32.73183911366379 34.48480008156468 18.75287600468997 88-89 14.809274037602115 31.35890846336475 34.9410398118059 18.89077768722723 90-91 13.334606681543542 31.890507460982185 35.68662583342566 19.08826002404861 92-93 12.743256121251834 33.4232381221681 34.74230994585321 19.091195810726855 94-95 13.008971211722365 33.384824782827835 33.81587011366891 19.79033389178089 96-97 12.839118692562302 32.417843184717185 32.96022911164842 21.7828090110721 98-99 14.01827291081759 31.77331529608506 32.90571912197576 21.302692671121594 100-101 14.773043218759922 31.559340906603513 33.51365792212507 20.153957952511504 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 21232.0 1 14136.5 2 7000.0 3 7136.0 4 6296.0 5 6156.0 6 10245.5 7 18886.0 8 25575.5 9 26703.5 10 22450.5 11 16159.0 12 12868.5 13 10849.0 14 9101.0 15 7480.0 16 6250.0 17 5444.5 18 4914.5 19 4592.5 20 4410.0 21 4345.5 22 4290.0 23 4400.0 24 4704.5 25 5101.0 26 5615.0 27 6129.0 28 6590.0 29 7186.5 30 7977.5 31 8770.0 32 9762.0 33 11182.0 34 12554.0 35 13473.5 36 14331.0 37 15069.0 38 14734.5 39 13805.5 40 13078.5 41 12432.0 42 11908.0 43 11723.5 44 12275.5 45 12985.5 46 15651.0 47 24113.5 48 45061.0 49 44985.5 50 25144.0 51 15967.0 52 9560.0 53 6982.0 54 6080.0 55 5755.0 56 5853.0 57 5802.0 58 5669.0 59 5814.0 60 6142.0 61 6085.0 62 5180.5 63 3519.0 64 2257.5 65 1782.5 66 1405.0 67 1109.5 68 867.0 69 610.0 70 455.0 71 356.0 72 335.5 73 373.5 74 310.0 75 205.0 76 155.5 77 113.5 78 78.5 79 55.5 80 37.5 81 25.5 82 15.5 83 8.5 84 6.0 85 3.5 86 0.5 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.019031435853865762 2 0.018895497026338148 3 1.3593882752761257E-4 4 0.10399320305862361 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.011011045029736618 12-13 0.0 14-15 0.0 16-17 8.836023789294817E-4 18-19 0.0 20-21 0.0 22-23 2.7187765505522514E-4 24-25 0.003806287170773152 26-27 0.004214103653355989 28-29 0.0015632965165675444 30-31 2.0390824129141885E-4 32-33 6.796941376380628E-4 34-35 0.0027187765505522514 36-37 0.08047578589634664 38-39 0.09706032285471537 40-41 0.07286321155480033 42-43 0.0768054375531011 44-45 0.07558198810535259 46-47 0.07388275276125744 48-49 0.06905692438402719 50-51 0.06926083262531861 52-53 0.05383177570093458 54-55 0.045267629566694986 56-57 0.025828377230246388 58-59 0.02331350892098556 60-61 0.013865760407816483 62-63 0.0019711129991503823 64-65 0.006796941376380629 66-67 0.14844519966015293 68-69 0.6861512319456244 70-71 0.8367034834324555 72-73 0.3400509770603229 74-75 0.053220050977060326 76-77 0.18528462192013592 78-79 0.01427357689039932 80-81 0.0036023789294817336 82-83 0.0016312659303313508 84-85 1.3593882752761257E-4 86-87 0.0016992353440951572 88-89 2.0390824129141885E-4 90-91 0.004282073067119797 92-93 0.004350042480883602 94-95 0.07497026338147833 96-97 0.03575191163976211 98-99 0.014953271028037384 100-101 0.01352591333899745 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 735625.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 28.35077695931616 #Duplication Level Percentage of deduplicated Percentage of total 1 75.7224123676894 21.467892238577253 2 10.24176029396074 5.807237235297224 3 4.323543029987785 3.67727412351569 4 2.3820798936501153 2.701352630565839 5 1.5040556653135588 2.1320573350850287 6 1.0125580402791399 1.7224084294989697 7 0.7346830973186331 1.4580185639502095 8 0.5897113327933066 1.3375019573123281 9 0.43114733114189074 1.1001025639627355 >10 2.6960608474442704 14.073822209891349 >50 0.18513523305531718 3.5764554296664373 >100 0.13738983084617543 7.456784568254821 >500 0.019487919268961054 3.59289223482837 >1k 0.015103137433444816 9.11262003308346 >5k 0.0024359899086201318 4.90659352828726 >10k+ 0.0024359899086201318 15.876986918223002 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 29837 4.056006796941376 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 27935 3.797451146983857 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26339 3.5804927782497877 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20792 2.8264401019541205 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10044 1.3653695836873407 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 9194 1.24982158028887 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 7529 1.0234834324553952 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7464 1.0146474086661001 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 5677 0.7717247238742566 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5659 0.7692778249787596 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4419 0.60071367884452 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 4228 0.5747493627867459 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3965 0.5389974511469838 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 3843 0.5224129141886151 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3678 0.499983007646559 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 3668 0.49862361937128297 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 3362 0.4570263381478335 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2536 0.34474086661002545 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 2398 0.3259813084112149 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 2372 0.32244689889549705 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 2143 0.29131690739167376 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 1989 0.27038232795242145 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1938 0.26344944774851314 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 1851 0.2516227697536109 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1847 0.2510790144435004 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 1715 0.23313508920985554 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1600 0.21750212404418012 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1577 0.214375531011045 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 1543 0.20975361087510622 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1498 0.20363636363636364 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1456 0.1979269328802039 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1378 0.18732370433305012 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1364 0.18542056074766355 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 1350 0.18351741716227699 No Hit GTACATGGGACTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAAAA 1315 0.17875955819881054 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 1299 0.17658453695836873 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 1215 0.1651656754460493 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 1179 0.16027187765505524 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1148 0.15605777400169923 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 1062 0.14436703483432456 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 1038 0.14110450297366184 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 991 0.13471537807986406 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 897 0.12193712829226848 No Hit CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 865 0.11758708581138487 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 840 0.11418861512319456 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 831 0.11296516567544604 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 775 0.10535259133389974 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 772 0.1049447748513169 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 760 0.10331350892098555 No Hit GAGTACATGGGACTCCCGACCCGGGGAGGTAGTGACGAAAAAAAAAAAAA 759 0.10317757009345795 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 759 0.10317757009345795 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 742 0.10086661002548852 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.7187765505522514E-4 0.0 12-13 0.0 0.0 0.0 4.75785896346644E-4 0.0 14-15 0.0 0.0 0.0 0.0010195412064570942 0.0 16-17 0.0 0.0 0.0 0.002650807136788445 0.0 18-19 0.0 0.0 0.0 0.005097706032285472 0.0 20-21 0.0 0.0 0.0 0.009447748513169074 0.0 22-23 0.0 0.0 0.0 0.020254885301614275 0.0 24-25 0.0 0.0 0.0 0.033712829226847915 0.0 26-27 0.0 0.0 0.0 0.045199660152931184 0.0 28-29 0.0 0.0 0.0 0.06851316907391673 0.0 30-31 0.0 0.0 0.0 0.12519966015293119 0.0 32-33 0.0 0.0 0.0 0.18983857264231097 0.0 34-35 0.0 0.0 0.0 0.2756159728122345 0.0 36-37 0.0 0.0 0.0 0.38851316907391675 0.0 38-39 0.0 0.0 0.0 0.5225488530161427 0.0 40-41 0.0 0.0 0.0 0.685267629566695 0.0 42-43 0.0 0.0 0.0 0.8246728971962616 0.0 44-45 0.0 0.0 0.0 0.9672727272727273 0.0 46-47 0.0 0.0 0.0 1.1364485981308412 0.0 48-49 0.0 0.0 0.0 1.336550552251487 0.0 50-51 0.0 0.0 0.0 1.521019541206457 0.0 52-53 0.0 0.0 0.0 1.687748513169074 0.0 54-55 0.0 0.0 0.0 1.8372812234494478 0.0 56-57 0.0 0.0 0.0 1.9940186915887852 0.0 58-59 0.0 0.0 0.0 2.1697875955819885 0.0 60-61 0.0 0.0 0.0 2.3861342395921836 0.0 62-63 0.0 0.0 0.0 2.569923534409516 0.0 64-65 0.0 0.0 0.0 2.7259133389974513 0.0 66-67 0.0 0.0 0.0 2.8867969413763808 0.0 68-69 0.0 0.0 0.0 3.064672897196262 0.0 70-71 0.0 0.0 0.0 3.305828377230246 0.0 72-73 0.0 0.0 0.0 3.592115548003399 0.0 74-75 0.0 0.0 0.0 3.867051826677995 0.0 76-77 0.0 0.0 0.0 4.121393372982158 0.0 78-79 0.0 0.0 0.0 4.416924384027188 0.0 80-81 0.0 0.0 0.0 4.74195412064571 0.0 82-83 0.0 0.0 0.0 5.082888700084962 0.0 84-85 0.0 0.0 0.0 5.501988105352591 0.0 86-87 0.0 0.0 0.0 5.924757858963467 0.0 88-89 0.0 0.0 0.0 6.28016992353441 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCACTA 180 0.0 50.09834 2 GATAGTC 100 1.2732926E-11 47.42286 7 ATAAGGC 55 4.9933158E-5 43.13515 3 TCACTAC 220 0.0 40.978394 3 CGTCACT 190 0.0 39.97581 1 TAGTCAA 170 0.0 39.05412 9 CTCGTCG 45 5.277034E-7 37.30937 66-67 TAGATAG 140 1.0913936E-11 37.26082 5 GTATATG 145 1.6370905E-11 36.01269 1 GGACCCG 130 8.411007E-9 32.83121 9 TCGCCGG 30 0.003760592 31.988258 68-69 CACTACC 300 0.0 31.643211 4 GCAGTAC 60 0.003970995 31.632444 3 GCATTCC 75 3.1068534E-4 31.615242 8 ACTGGGC 105 1.9247E-6 31.615238 8 AACGTAT 40 4.250503E-4 29.971466 64-65 GCTTATG 80 4.5080192E-4 29.669548 1 ACTACCT 320 0.0 29.63929 5 GTCTATA 65 0.0058600125 29.213093 1 CGACTAA 50 4.994155E-5 28.466297 50-51 >>END_MODULE