FastQCFastQC Report
Fri 27 May 2016
SRR939499_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939499_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3435940
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86010.25032451090531266No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74220.2160107568816685No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44590.1297752580079978No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA93600.039.606421
GTATCAA146950.034.9642371
TATCAAC180900.027.6439132
ATCAACG182650.027.059843
TCAACGC185100.026.9400464
CAACGCA186850.026.3052275
AACGCAG190350.025.739396
GTACATG124250.024.4408051
ACGCAGA210500.022.7577617
TACATGG130500.022.6182562
GAGTACT107250.022.43769512-13
CGCAGAG214100.022.2371238
ACATGGG132450.021.6952423
GTACTTT114600.021.32714314-15
GCAGAGT213850.020.8199569
AGAGTAC175350.019.8256410-11
CAGAGTA208250.018.95385710-11
AGTACTT115200.018.60287312-13
ATACCGT3406.7484507E-1018.5478746
ACTTTTT144100.018.52946516-17
Produced by FastQC (version 0.11.5)