FastQCFastQC Report
Fri 27 May 2016
SRR939499_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939499_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3435940
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125990.36668277094477786No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122000.35507022823448603No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75830.22069651972968096No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA106200.039.009651
GTATCAA162450.032.388321
ATCAACG198900.026.154363
TATCAAC200100.026.144522
TCAACGC200500.025.9929074
CAACGCA203550.025.6034285
AACGCAG208350.025.059056
GTACATG140150.021.8486481
GAGTACT129250.021.79267312-13
ACGCAGA237900.021.747277
CGCAGAG241700.021.3661568
GTACTTT133650.020.96886614-15
TACATGG144550.020.6529052
GCAGAGT250000.020.050369
ACATGGG142200.019.9240743
AGTACTT132500.019.5417512-13
AGAGTAC207700.019.17245910-11
CGCGGAA5900.018.4699282-83
ACTTTTT158100.018.1605916-17
CAGAGTA245050.018.1546310-11
Produced by FastQC (version 0.11.5)