##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR939499_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3435940 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.982066625144792 31.0 31.0 31.0 30.0 34.0 2 31.251916215067784 31.0 31.0 34.0 30.0 34.0 3 31.241555149391434 31.0 31.0 34.0 28.0 34.0 4 35.17567419687189 35.0 35.0 37.0 33.0 37.0 5 34.78893548781411 35.0 35.0 37.0 32.0 37.0 6 34.74623916599242 35.0 35.0 37.0 32.0 37.0 7 34.61676251622554 35.0 35.0 37.0 30.0 37.0 8 34.63805537931396 35.0 35.0 37.0 30.0 37.0 9 35.99593415484554 37.0 35.0 39.0 30.0 39.0 10-11 35.84725242582816 37.0 35.0 39.0 30.0 39.0 12-13 35.80344301704919 37.0 35.0 39.0 30.0 39.0 14-15 36.6821399675198 38.0 36.0 40.0 31.0 41.0 16-17 36.679669027980694 38.0 35.5 40.0 31.0 41.0 18-19 36.62349488058581 38.0 35.0 40.0 30.0 41.0 20-21 36.50682200504083 38.0 35.0 40.0 30.0 41.0 22-23 36.36665293922478 38.0 35.0 40.0 30.0 41.0 24-25 36.14167374866849 38.0 34.0 40.0 29.0 41.0 26-27 35.771367951710445 38.0 34.0 40.0 27.5 41.0 28-29 35.495148634725865 38.0 34.0 40.0 27.0 41.0 30-31 35.284799792778685 38.0 34.0 40.0 27.0 41.0 32-33 35.09686082993301 38.0 33.0 40.0 27.0 41.0 34-35 35.06639609539165 38.0 33.0 40.0 26.0 41.0 36-37 35.08796733935982 38.0 33.0 40.0 26.0 41.0 38-39 35.070078493803734 38.0 33.0 40.0 26.0 41.0 40-41 34.85348594562187 38.0 33.0 40.0 25.5 41.0 42-43 34.55659033626897 38.0 33.0 40.0 24.5 41.0 44-45 34.382016275022266 37.0 33.0 40.0 24.0 41.0 46-47 34.07196720548089 37.0 33.0 40.0 24.0 41.0 48-49 33.77692669254993 37.0 32.5 40.0 23.0 41.0 50-51 33.51407096747906 37.0 32.0 40.0 23.0 41.0 52-53 33.13458514991531 37.0 31.0 39.5 21.5 41.0 54-55 32.89418601605383 36.0 31.0 39.0 20.5 40.5 56-57 32.48810602047766 36.0 31.0 39.0 19.0 40.0 58-59 32.03438942472802 35.5 30.0 39.0 17.5 40.0 60-61 31.73390993439932 35.0 30.0 39.0 16.5 40.0 62-63 31.330887908403522 35.0 29.0 38.0 13.0 40.0 64-65 30.877651530585517 34.5 29.0 38.0 10.5 40.0 66-67 29.14893216994476 33.5 25.5 37.0 7.0 39.5 68-69 25.449289714022946 29.5 16.0 35.0 4.5 38.0 70-71 24.824883583531726 29.5 15.0 35.0 2.0 38.0 72-73 26.675376025192524 31.0 21.5 35.0 3.5 38.0 74-75 27.306333346915252 31.5 24.0 35.0 2.0 37.5 76-77 27.378433121649387 31.5 25.5 34.5 2.0 36.0 78-79 28.221344377375623 32.5 26.0 35.0 2.0 37.0 80-81 28.08582192937013 33.0 26.0 35.0 2.0 36.5 82-83 27.692651355960813 33.0 26.0 35.0 2.0 36.0 84-85 27.364633841103164 32.0 26.0 35.0 2.0 36.0 86-87 26.91781084652235 32.0 25.0 34.5 2.0 35.0 88-89 26.431125397998798 32.0 24.5 34.0 2.0 35.0 90-91 25.98097638491941 32.0 24.0 34.0 2.0 35.0 92-93 25.413830567472075 31.5 21.5 34.0 2.0 35.0 94-95 24.022719692427692 31.0 14.5 34.0 2.0 35.0 96-97 23.214366083226132 30.0 4.5 34.0 2.0 35.0 98-99 22.86953555649982 30.0 2.0 34.0 2.0 35.0 100-101 21.593298049442076 29.0 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.3302708893340558 1101 2 0.2048044383834764 1101 3 0.34598618911981305 1101 4 0.24279208106288053 1101 5 0.34462832029001333 1101 6 0.4582391123537093 1101 7 0.5602285609840649 1101 8 0.4620647227065149 1101 9 0.6024312080371317 1101 10-11 0.7835635272339729 1101 12-13 0.6445028338426866 1101 14-15 0.7458902295890582 1101 16-17 0.6389894493782364 1101 18-19 0.6958755141625019 1101 20-21 0.7156396132470277 1101 22-23 0.7954851718069733 1101 24-25 0.7931194510072004 1101 26-27 0.886912583692741 1101 28-29 0.7013419093796855 1101 30-31 0.7774219769493911 1101 32-33 0.7282213063484662 1101 34-35 0.8032674520856844 1101 36-37 0.8614820506658418 1101 38-39 0.7544216165914008 1101 40-41 0.9163251309562099 1101 42-43 0.9520186177528771 1101 44-45 0.6042147288436226 1101 46-47 0.6680372316966796 1101 48-49 0.6570076341324125 1101 50-51 0.7072443702771949 1101 52-53 0.8134249164189598 1101 54-55 0.7336758424266492 1101 56-57 0.6504024619899482 1101 58-59 0.6787686352213562 1101 60-61 0.5397857071913954 1101 62-63 0.3794627585997432 1101 64-65 0.08206527562673571 1101 66-67 -2.973175485874581 1101 68-69 -8.018508207707264 1101 70-71 -11.360234528412278 1101 72-73 -10.903187871958526 1101 74-75 -9.520768372614118 1101 76-77 -4.5558462473738714 1101 78-79 -1.435817757518425 1101 80-81 -0.7339026134951325 1101 82-83 -0.5165888152521241 1101 84-85 -0.49744799392721717 1101 86-87 -0.5862315683111952 1101 88-89 -0.5789619252737523 1101 90-91 -0.6451865231672826 1101 92-93 -1.3067650440475589 1101 94-95 -4.958343408071311 1101 96-97 -1.9139328816197008 1101 98-99 -1.0930276806144263 1101 100-101 -0.8084629487005586 1102 1 0.4882126973116705 1102 2 0.3942618356231584 1102 3 0.4285022619438337 1102 4 0.3063123304747535 1102 5 0.5138337892328906 1102 6 0.6081376051502687 1102 7 0.4905728557351452 1102 8 0.5835620100667782 1102 9 0.716762528909392 1102 10-11 0.7549958295805723 1102 12-13 0.7141563967285265 1102 14-15 0.6504925355878797 1102 16-17 0.7298017382449515 1102 18-19 0.7690876441542898 1102 20-21 0.9521149090801728 1102 22-23 0.8497041308361446 1102 24-25 0.9108969499377153 1102 26-27 1.0082822814291177 1102 28-29 0.9219160422837263 1102 30-31 0.7787366397584421 1102 32-33 0.7057351653973001 1102 34-35 0.9171461222427055 1102 36-37 1.1363640336643215 1102 38-39 0.996215617838736 1102 40-41 1.1587894878154117 1102 42-43 1.1807382871624483 1102 44-45 0.962136337397844 1102 46-47 0.9747853693710553 1102 48-49 0.8828685750446752 1102 50-51 0.8889563428223042 1102 52-53 0.9514129246265952 1102 54-55 0.7546629073144047 1102 56-57 0.8217118675079931 1102 58-59 0.7409343670695883 1102 60-61 0.742212868546698 1102 62-63 0.7691372486323118 1102 64-65 0.7220014060021143 1102 66-67 -2.5397221802083365 1102 68-69 -7.482708844431173 1102 70-71 -11.166039958316539 1102 72-73 -9.287388046204057 1102 74-75 -7.753062251780758 1102 76-77 -3.9252944154493967 1102 78-79 -1.0731764550704845 1102 80-81 -0.3714064476449437 1102 82-83 -0.027441890631340726 1102 84-85 -0.10248536966165744 1102 86-87 -0.049531732423009345 1102 88-89 -0.08138330564929674 1102 90-91 -0.20588630056555246 1102 92-93 -0.7066562596107708 1102 94-95 -4.413656162476926 1102 96-97 -1.1981184673927814 1102 98-99 -0.5662449794822386 1102 100-101 -0.3078820282461585 1103 1 0.373884013085803 1103 2 0.2533749073668332 1103 3 0.3875266064897751 1103 4 0.22495943110379812 1103 5 0.4528227035563859 1103 6 0.37685508434082493 1103 7 0.3951892647739257 1103 8 0.3925308369301206 1103 9 0.5714468565456059 1103 10-11 0.5767919538229123 1103 12-13 0.49220236301083986 1103 14-15 0.6642785786952103 1103 16-17 0.5845256274953456 1103 18-19 0.471631044828861 1103 20-21 0.6521597950001805 1103 22-23 0.6883618261598201 1103 24-25 0.7829578573005236 1103 26-27 0.6695043848466824 1103 28-29 0.8330459644443948 1103 30-31 0.7955043278269116 1103 32-33 0.7085257082170173 1103 34-35 0.7888876386521844 1103 36-37 0.7451817580762281 1103 38-39 0.761606888605634 1103 40-41 0.921954053010019 1103 42-43 0.7825685341394717 1103 44-45 0.6851213993501872 1103 46-47 0.7868712403145963 1103 48-49 0.791457051022789 1103 50-51 0.5735637072808046 1103 52-53 0.6949034384322132 1103 54-55 0.7599071126668164 1103 56-57 0.556451817291034 1103 58-59 0.6125229276300281 1103 60-61 0.4725294996225209 1103 62-63 0.5224088492231118 1103 64-65 0.36648685733644015 1103 66-67 -2.8692028952693462 1103 68-69 -7.699001995318294 1103 70-71 -11.713616236579586 1103 72-73 -9.550194433888333 1103 74-75 -7.791418652051288 1103 76-77 -3.887761563976973 1103 78-79 -1.1106616910737124 1103 80-81 -0.41920944698143003 1103 82-83 -0.14045907701877525 1103 84-85 -0.22893978972849283 1103 86-87 -0.08854858250302655 1103 88-89 -0.02559148870297534 1103 90-91 0.08861352302469427 1103 92-93 0.058342630035578935 1103 94-95 0.8721413528245563 1103 96-97 1.22646250468393 1103 98-99 0.8097658214450014 1103 100-101 0.6142493924707182 1104 1 0.3929520524797674 1104 2 0.23384400894550694 1104 3 0.29368000275355044 1104 4 0.12547157164339495 1104 5 0.4289420578768741 1104 6 0.501876201349944 1104 7 0.5010325713018773 1104 8 0.3815599096259561 1104 9 0.6020743484290989 1104 10-11 0.6403720671873714 1104 12-13 0.5900952132517574 1104 14-15 0.72227775609133 1104 16-17 0.7146297368126753 1104 18-19 0.6697816805128198 1104 20-21 0.7443912489678155 1104 22-23 0.5316319448871667 1104 24-25 0.6854651688632671 1104 26-27 0.8365496576084723 1104 28-29 0.9214070366008897 1104 30-31 0.8408358470390596 1104 32-33 0.723568524714878 1104 34-35 0.7461015384160987 1104 36-37 0.790917073221479 1104 38-39 0.758857924417434 1104 40-41 0.8520219908521369 1104 42-43 0.8223055997803357 1104 44-45 0.6133470033883484 1104 46-47 0.702598563771474 1104 48-49 0.6004703033682475 1104 50-51 0.6147090150593044 1104 52-53 0.6631394150229895 1104 54-55 0.5972562835588704 1104 56-57 0.6092262373671815 1104 58-59 0.8559665675469041 1104 60-61 0.6744935258167821 1104 62-63 0.6323803909328944 1104 64-65 0.3091357776773407 1104 66-67 -2.727317244001089 1104 68-69 -7.311392882280227 1104 70-71 -8.87767187747869 1104 72-73 -8.261316024726792 1104 74-75 -7.074628091006236 1104 76-77 -3.6253132611663617 1104 78-79 -0.6362520418147497 1104 80-81 0.04152154287052312 1104 82-83 0.2906450292418121 1104 84-85 0.1839532524080525 1104 86-87 0.43070082372437923 1104 88-89 0.4918117458080786 1104 90-91 0.32320394814651365 1104 92-93 0.337887134365797 1104 94-95 1.2974011336423992 1104 96-97 1.5522169007069024 1104 98-99 1.1441239339936473 1104 100-101 1.010957007058721 1105 1 0.4413817316235189 1105 2 0.31502050781580593 1105 3 0.4691785483483315 1105 4 0.35596068586642815 1105 5 0.41276533384547776 1105 6 0.4248595282557446 1105 7 0.5115059140908471 1105 8 0.48094608324513644 1105 9 0.7608050628737075 1105 10-11 0.8346625575001951 1105 12-13 0.7758366247104647 1105 14-15 0.8896785946386387 1105 16-17 0.9671164802417778 1105 18-19 0.9334741666671036 1105 20-21 1.0160749900684536 1105 22-23 1.0694342236528556 1105 24-25 1.039268715302832 1105 26-27 1.1692576412790459 1105 28-29 1.1441104188590714 1105 30-31 1.2001123655136112 1105 32-33 1.190083075030934 1105 34-35 1.2243086534388823 1105 36-37 1.1470020263321032 1105 38-39 1.0646921022459352 1105 40-41 1.04347998848948 1105 42-43 1.114367706319797 1105 44-45 1.1038489297853857 1105 46-47 1.236915890577734 1105 48-49 0.9526286840503033 1105 50-51 1.1888662438646165 1105 52-53 1.2728808657579052 1105 54-55 1.3037128201885153 1105 56-57 1.3000195188310784 1105 58-59 1.45590399646456 1105 60-61 1.5031929987366688 1105 62-63 1.5010495805324169 1105 64-65 1.4660611064717841 1105 66-67 2.2358662765093946 1105 68-69 -4.716741592479618 1105 70-71 -5.46546506515368 1105 72-73 -1.5372152893472801 1105 74-75 1.3003317947676827 1105 76-77 1.4326399534060634 1105 78-79 1.3800239223910715 1105 80-81 1.328999064852507 1105 82-83 1.5560180974696856 1105 84-85 1.4905804035852697 1105 86-87 1.647203608103684 1105 88-89 1.673822999799608 1105 90-91 1.5881730807616563 1105 92-93 1.5758960873125467 1105 94-95 2.5319046161903813 1105 96-97 2.443312439774722 1105 98-99 2.206108615590473 1105 100-101 2.0336416653945406 1106 1 0.09464520866237436 1106 2 0.1236338352312707 1106 3 0.17400092904532372 1106 4 -0.035401398140123774 1106 5 0.21502157959477586 1106 6 0.21018336971195595 1106 7 0.2582394988793979 1106 8 0.23863363580559138 1106 9 0.40119704217705987 1106 10-11 0.3303578977074082 1106 12-13 0.26972627507198865 1106 14-15 0.39192396102537685 1106 16-17 0.37915666469402964 1106 18-19 0.3812358613725664 1106 20-21 0.3871537313336475 1106 22-23 0.3769271527441873 1106 24-25 0.42857719268899075 1106 26-27 0.39868921455673245 1106 28-29 0.5590007661226366 1106 30-31 0.6626903709493703 1106 32-33 0.5549585147920979 1106 34-35 0.5573947244291872 1106 36-37 0.5027517999100297 1106 38-39 0.47372275848881173 1106 40-41 0.5339492403779218 1106 42-43 0.5269219017256006 1106 44-45 0.566741705577229 1106 46-47 0.5133827326230644 1106 48-49 0.3982194483312824 1106 50-51 0.2534262904312712 1106 52-53 0.6444411220586304 1106 54-55 0.5943981157720586 1106 56-57 0.5992453509736819 1106 58-59 0.7216335101915838 1106 60-61 0.485626856341991 1106 62-63 0.5803963530039908 1106 64-65 0.5206250024507284 1106 66-67 1.1732470147976954 1106 68-69 -5.506983149532363 1106 70-71 -6.626291527067174 1106 72-73 -2.521707391011301 1106 74-75 0.2556942508524962 1106 76-77 0.526614154084335 1106 78-79 0.5042970462493948 1106 80-81 0.5847158076837395 1106 82-83 0.5594498695140686 1106 84-85 0.47835689476540466 1106 86-87 0.5313910334561598 1106 88-89 0.3908246813279632 1106 90-91 0.31525543611454054 1106 92-93 0.4981006225782636 1106 94-95 1.248956530992043 1106 96-97 1.4976142020148195 1106 98-99 1.2207068313208538 1106 100-101 1.2743328373232679 1107 1 -0.055800469654325724 1107 2 -0.07721503230051141 1107 3 -0.09081830585539308 1107 4 -0.05370470420023565 1107 5 0.12337146187041981 1107 6 0.11333649719825445 1107 7 0.017479788114812322 1107 8 -0.07374989727576065 1107 9 0.00976696734981175 1107 10-11 0.05991022859340234 1107 12-13 0.14663939259726533 1107 14-15 -0.07896506011435633 1107 16-17 -0.014088904726762053 1107 18-19 0.1275801103249279 1107 20-21 0.08822510387935978 1107 22-23 0.0352332093405181 1107 24-25 0.288167876320351 1107 26-27 0.2178827868842248 1107 28-29 0.16525672356600296 1107 30-31 0.05235331419194722 1107 32-33 0.16495509596703783 1107 34-35 0.1501897264727461 1107 36-37 0.22250800110295899 1107 38-39 0.08675910425724709 1107 40-41 0.28761561370096445 1107 42-43 0.3299695450929079 1107 44-45 0.10881780468375979 1107 46-47 0.03442767610341235 1107 48-49 0.13210960696898155 1107 50-51 0.05204328424292015 1107 52-53 0.07748994020738564 1107 54-55 0.041961253240479834 1107 56-57 0.2573139891628031 1107 58-59 -0.03251719668215358 1107 60-61 -0.009480214083691152 1107 62-63 0.11593792373603407 1107 64-65 0.1588120121817198 1107 66-67 1.0198501377466656 1107 68-69 -5.453251248728936 1107 70-71 -6.286574944851118 1107 72-73 -2.5636004141460553 1107 74-75 -0.04771465539783648 1107 76-77 0.06437831283881223 1107 78-79 0.2148622708894159 1107 80-81 0.09833117298097349 1107 82-83 0.22331288858984877 1107 84-85 0.1436538155014233 1107 86-87 0.08106610438271034 1107 88-89 0.05726655672938108 1107 90-91 0.14345631108050938 1107 92-93 0.17601898722620746 1107 94-95 0.877082803818503 1107 96-97 1.1793255983094042 1107 98-99 0.8162215227718903 1107 100-101 0.5278007288578763 1108 1 0.06383113075277436 1108 2 0.049921794091233096 1108 3 0.06583200536567446 1108 4 0.03347516992270272 1108 5 0.010237334109483243 1108 6 0.18029796572384527 1108 7 0.18212049790643903 1108 8 0.06015539842410078 1108 9 0.2776537920588993 1108 10-11 0.30397676553591424 1108 12-13 0.25678710171901287 1108 14-15 0.34056040878957106 1108 16-17 0.15153720560678607 1108 18-19 0.22994047353838454 1108 20-21 0.3065229756168151 1108 22-23 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86-87 -5.436973289284818 2315 88-89 -3.8680218065353316 2315 90-91 -3.509866573097465 2315 92-93 -4.291241205537837 2315 94-95 -2.2838078019686385 2315 96-97 -1.1070910274577912 2315 98-99 -2.8176369070050384 2315 100-101 -1.9722230192224863 2316 1 -0.4051247870113528 2316 2 -0.34796205907245437 2316 3 -0.8419384183852578 2316 4 -0.3079349767479087 2316 5 -0.1253026788853262 2316 6 -2.283669014654322 2316 7 -0.5580734746265321 2316 8 -0.45764160365725104 2316 9 0.020001895887403975 2316 10-11 -0.05380164957931299 2316 12-13 -0.4751346996939674 2316 14-15 -1.1423269808375736 2316 16-17 -1.432085737246389 2316 18-19 -0.24312748974761433 2316 20-21 -3.9584653886450383 2316 22-23 -4.138422719312928 2316 24-25 -2.656814651586174 2316 26-27 -2.0418099638801976 2316 28-29 -1.3893821915568054 2316 30-31 -0.44500564285660715 2316 32-33 -2.0994686160991662 2316 34-35 -2.483360089768503 2316 36-37 -4.83765823399299 2316 38-39 -2.2050489374549898 2316 40-41 -1.7754580675214342 2316 42-43 -3.159521783762763 2316 44-45 -0.8187499500991535 2316 46-47 -0.8577029973228107 2316 48-49 -0.5566328572239954 2316 50-51 -0.02045374207410333 2316 52-53 -2.537452596894653 2316 54-55 -0.959000091003734 2316 56-57 0.05174154078297022 2316 58-59 0.049461283618750684 2316 60-61 -1.1370980711030718 2316 62-63 -0.6293799737412051 2316 64-65 0.4831606072871075 2316 66-67 1.5137270500174047 2316 68-69 3.8017282524201974 2316 70-71 3.946625975428706 2316 72-73 2.185972170104254 2316 74-75 1.1265412263075092 2316 76-77 -0.26859211289068696 2316 78-79 0.22493191057184347 2316 80-81 -0.08987866683137824 2316 82-83 0.2055463079946982 2316 84-85 0.35662204546704857 2316 86-87 -0.05192142646868092 2316 88-89 0.3853841623277283 2316 90-91 0.49074123691888616 2316 92-93 0.8306316987580331 2316 94-95 1.4285710404078138 2316 96-97 1.4425468567004671 2316 98-99 0.6890887961244765 2316 100-101 0.4189138901573557 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11.0 3 51.0 4 96.0 5 273.0 6 607.0 7 1494.0 8 3188.0 9 5362.0 10 8212.0 11 12197.0 12 16112.0 13 20483.0 14 25040.0 15 30775.0 16 36503.0 17 41758.0 18 40291.0 19 35712.0 20 35526.0 21 35668.0 22 38102.0 23 43476.0 24 52144.0 25 65623.0 26 82688.0 27 102983.0 28 125974.0 29 150863.0 30 176005.0 31 206817.0 32 238404.0 33 275674.0 34 318995.0 35 366639.0 36 388169.0 37 320120.0 38 126598.0 39 7307.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.482293349635384 19.83037130838537 15.381249727086155 23.306085614893092 2 16.30100212557979 20.543516166574197 38.75145620554607 24.404025502299945 3 19.399116399005802 24.1114804100188 30.114233659493472 26.375169531481923 4 13.765752603363271 17.292938759116865 35.58868897594254 33.35261966157733 5 14.159111229079938 37.151041230104404 33.74442946550809 14.945418075307574 6 32.77443727189648 35.811510096218214 16.625377625918965 14.788675005966345 7 28.977601471504162 32.4658754227373 20.125351432213602 18.43117167354494 8 26.636087941000135 35.29913211522902 18.78312776125311 19.28165218251774 9 26.16110875044384 16.80518286116754 19.97176900644365 37.06193938194497 10-11 25.62103820206406 26.46722585376927 26.42758604632211 21.48414989784455 12-13 26.27174805147936 23.687302455805398 27.294044715565462 22.746904777149776 14-15 23.101669412155044 25.140762062201322 25.598526167511654 26.159042358131977 16-17 22.640008370372083 28.430611987414757 26.246180617553595 22.683199024659565 18-19 22.48550012631363 26.667194428695662 28.687790535907414 22.15951490908329 20-21 23.210052102220704 25.704523771965615 28.88407765215787 22.20134647365581 22-23 22.954111538618253 25.66259888123774 28.851464228129707 22.531825352014295 24-25 22.956847325622682 25.91273421538211 28.816335558828154 22.314082900167058 26-27 22.656934404291597 26.170761535609323 28.75310760476215 22.41919645533693 28-29 22.414117321034453 26.28163964642657 28.942420602877284 22.3618224296617 30-31 22.540954083489616 25.957802453803275 28.828528464592416 22.67271499811469 32-33 22.497201750072293 26.069298217400917 28.774042719099953 22.659457313426838 34-35 23.084579330033183 26.078129516128612 28.506596949647555 22.330694204190646 36-37 22.909631887783235 25.902319869238145 28.50122774963259 22.68682049334603 38-39 22.594111235837538 26.163624238543225 28.718526768871122 22.52373775674812 40-41 22.84549620157893 25.966607578659723 28.73750341102481 22.450392808736535 42-43 22.74833010284067 26.118686154311405 28.264329563276043 22.868654179571884 44-45 22.81862565517667 26.24716811750042 28.193743660902125 22.74046256642078 46-47 22.929679767203005 26.4127054231463 27.88068003129481 22.77693477835589 48-49 23.046492673905966 26.50828969918476 27.65725955939282 22.787958067516456 50-51 22.819503271759302 26.632066809993532 27.699254227372887 22.849175690874283 52-53 23.26356338633884 26.608912026603292 27.35640868393432 22.771115903123547 54-55 22.928385808391795 26.453396853447764 27.560508995786716 23.05770834237373 56-57 22.94815207327654 26.782141463783898 27.475121263822327 22.794585199117236 58-59 23.13559761125491 26.459622104158882 27.427007979172412 22.977772305413797 60-61 23.037894548415476 26.52231271934516 27.740698455388024 22.69909427685134 62-63 22.945178617450306 26.522344443986857 27.75910309931105 22.77337383925179 64-65 22.789732255055863 26.491284315085167 27.570250613505458 23.148732816353508 66-67 22.253586497397006 26.670815012608713 28.094512141657148 22.981086348337136 68-69 22.16857864522997 26.23119564974994 28.74471682412027 22.855508880899816 70-71 21.802235679413407 26.33315623411284 28.989952923326612 22.87465516314715 72-73 22.7523234868004 26.471668112339902 27.741048986725104 23.034959414134597 74-75 22.466645631559658 26.836215458763295 27.465272034213868 23.23186687546318 76-77 22.78300542603595 26.73800667331689 27.22211759486976 23.2568703057774 78-79 22.77030591580821 26.85585843216784 27.240878228175458 23.132957423848495 80-81 22.669908514521882 27.011652403787593 27.135753785652405 23.182685296038112 82-83 22.69942141015268 26.97983666769501 27.041973957636046 23.278767964516263 84-85 22.675468721805387 26.907862186184854 26.905155503297497 23.51151358871226 86-87 22.74265494370016 26.855452819444846 27.12345858847385 23.278433648381146 88-89 22.615078863260752 26.910382400265664 26.872401256666528 23.602137479807055 90-91 22.57863033729318 27.034402809922742 26.99174928634295 23.395217566441122 92-93 22.65232564941897 26.865221388762528 27.040198447034953 23.442254514783553 94-95 22.12031967675877 26.69549165462344 27.435893060519785 23.748295608098008 96-97 22.067228552750986 26.582080378705736 27.547251434250548 23.80343963429273 98-99 22.33610362876489 26.617511193441175 26.93481636119871 24.111568816595227 100-101 22.159406363209165 26.52033273534809 27.16900628939604 24.151254612046703 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1435.0 1 1047.5 2 684.0 3 754.0 4 745.5 5 765.5 6 1170.5 7 2281.5 8 3532.0 9 4387.5 10 4427.5 11 3939.5 12 3765.5 13 3845.0 14 4071.5 15 4269.0 16 4491.5 17 4843.5 18 5167.0 19 5549.0 20 6204.0 21 7008.0 22 8239.0 23 9836.5 24 11745.0 25 14452.5 26 18141.0 27 22385.5 28 26494.5 29 30786.0 30 35936.0 31 41863.0 32 48137.0 33 54177.0 34 60178.5 35 65935.0 36 72268.0 37 78957.0 38 83695.5 39 86456.0 40 89092.0 41 94185.5 42 99807.5 43 104713.5 44 110025.0 45 114613.0 46 118406.0 47 123902.0 48 131524.0 49 133335.0 50 130497.0 51 128759.5 52 127195.5 53 128045.0 54 127798.0 55 121928.0 56 113713.5 57 104134.0 58 93839.5 59 82823.0 60 70106.0 61 57375.0 62 46173.0 63 36535.5 64 27946.0 65 20848.5 66 15553.5 67 11806.5 68 8271.0 69 5464.0 70 3965.0 71 2943.0 72 2137.5 73 1615.0 74 1235.0 75 910.5 76 589.0 77 341.5 78 195.5 79 102.5 80 55.0 81 33.0 82 22.5 83 11.5 84 9.0 85 4.0 86 1.0 87 1.0 88 1.0 89 1.0 90 0.5 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022992252484036392 2 0.01868484315791312 3 0.0 4 0.0 5 1.1641646827360197E-4 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.346973462866057E-4 18-19 0.001396997619283224 20-21 5.820823413680099E-5 22-23 0.0 24-25 0.0 26-27 4.0745763895760694E-4 28-29 0.017869927879997905 30-31 0.004991356077230685 32-33 0.009036828349738355 34-35 0.009808087452050968 36-37 0.1001327147738319 38-39 0.10299947030506937 40-41 0.1179735385367614 42-43 0.10311588677334295 44-45 0.09818273893024906 46-47 0.09957973654953228 48-49 0.10237373178809875 50-51 0.10068569299813153 52-53 0.07801358580184753 54-55 0.06627007456474793 56-57 0.03725326984755264 58-59 0.014435642065926645 60-61 0.008367433657165142 62-63 0.0018481114338434314 64-65 0.004409273735862675 66-67 0.42189328102353363 68-69 0.8135182802959307 70-71 0.904235813198135 72-73 0.28009802266628636 74-75 0.15039552495095956 76-77 0.013591622670943031 78-79 0.007930871901139135 80-81 0.0011496126242018195 82-83 0.0 84-85 0.0 86-87 1.8917676094460322E-4 88-89 7.130508681758122E-4 90-91 0.0033760775799344576 92-93 0.0017753511411724304 94-95 0.22306850527075558 96-97 0.2282635901674651 98-99 0.01298043621250662 100-101 0.006883123686676717 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3435940.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.800500444839926 #Duplication Level Percentage of deduplicated Percentage of total 1 70.78224475188658 33.83426721749323 2 15.132867830988515 14.467173109737406 3 5.817461166027474 8.342326651636057 4 2.8130928936336583 5.378689924540469 5 1.480551342899716 3.538554756244311 6 0.9012248452357436 2.5847399169355176 7 0.6048068927554168 2.0237050502338283 8 0.41651951559834866 1.59278730325147 9 0.3113956667167728 1.3396381834874693 >10 1.5416682120498733 13.433158710919368 >50 0.12199162439882658 4.030178486718274 >100 0.07008184560054057 6.103437967218058 >500 0.004739320608260527 1.554430464146924 >1k 0.0011694427458275568 0.824200608978394 >5k 6.154961820145035E-5 0.22309963653470272 >10k+ 1.230992364029007E-4 0.7296120119245803 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12599 0.36668277094477786 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12200 0.35507022823448603 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7583 0.22069651972968096 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.9104117068400497E-5 2 0.0 0.0 0.0 0.0 2.9104117068400497E-5 3 0.0 0.0 0.0 0.0 2.9104117068400497E-5 4 0.0 0.0 0.0 0.0 2.9104117068400497E-5 5 0.0 2.9104117068400497E-5 0.0 0.0 2.9104117068400497E-5 6 0.0 2.9104117068400497E-5 0.0 0.0 1.1641646827360199E-4 7 0.0 2.9104117068400497E-5 0.0 0.0 1.1641646827360199E-4 8 0.0 2.9104117068400497E-5 0.0 0.0 1.1641646827360199E-4 9 0.0 2.9104117068400497E-5 0.0 0.0 1.1641646827360199E-4 10-11 0.0 2.9104117068400497E-5 0.0 2.9104117068400497E-5 1.1641646827360199E-4 12-13 0.0 2.9104117068400497E-5 0.0 2.9104117068400497E-5 1.1641646827360199E-4 14-15 0.0 2.9104117068400497E-5 0.0 2.9104117068400497E-5 1.4552058534200246E-4 16-17 0.0 2.9104117068400497E-5 0.0 1.0186440973940174E-4 2.0372881947880347E-4 18-19 0.0 2.9104117068400497E-5 0.0 1.7462470241040297E-4 2.0372881947880347E-4 20-21 0.0 4.365617560260074E-5 0.0 3.2014528775240543E-4 2.0372881947880347E-4 22-23 0.0 5.8208234136800994E-5 0.0 4.947699901628085E-4 2.3283293654720398E-4 24-25 0.0 5.8208234136800994E-5 0.0 7.276029267100124E-4 2.619370536156045E-4 26-27 0.0 5.8208234136800994E-5 0.0 0.0010768523315308183 2.619370536156045E-4 28-29 0.0 5.8208234136800994E-5 0.0 0.001993632019185434 2.619370536156045E-4 30-31 0.0 5.8208234136800994E-5 0.0 0.0044820340285336765 2.619370536156045E-4 32-33 0.0 5.8208234136800994E-5 0.0 0.010506586261692579 3.2014528775240543E-4 34-35 0.0 5.8208234136800994E-5 0.0 0.02053295459175655 3.2014528775240543E-4 36-37 0.0 5.8208234136800994E-5 0.0 0.036234625750158615 3.2014528775240543E-4 38-39 0.0 5.8208234136800994E-5 0.0 0.05903770147325041 3.2014528775240543E-4 40-41 0.0 5.8208234136800994E-5 0.0 0.08315046246442022 3.2014528775240543E-4 42-43 0.0 5.8208234136800994E-5 0.0 0.11011542692829328 3.346973462866057E-4 44-45 0.0 5.8208234136800994E-5 0.0 0.14461835771288206 3.638014633550062E-4 46-47 0.0 5.8208234136800994E-5 0.0 0.18858012654470102 4.511138145602077E-4 48-49 0.0 5.8208234136800994E-5 0.0 0.23565603590283882 5.23874107231209E-4 50-51 0.0 5.8208234136800994E-5 0.0 0.2855695966751457 5.23874107231209E-4 52-53 0.0 5.8208234136800994E-5 0.0 0.3365163536033807 5.23874107231209E-4 54-55 0.0 5.8208234136800994E-5 0.0 0.3927018516039279 5.23874107231209E-4 56-57 0.0 5.8208234136800994E-5 0.0 0.4596267688027148 5.23874107231209E-4 58-59 0.0 5.8208234136800994E-5 0.0 0.5274248095135539 5.23874107231209E-4 60-61 0.0 5.8208234136800994E-5 0.0 0.596838128721689 5.23874107231209E-4 62-63 0.0 5.8208234136800994E-5 0.0 0.6653637723592379 5.529782242996095E-4 64-65 0.0 5.8208234136800994E-5 0.0 0.7400449367567536 5.529782242996095E-4 66-67 0.0 5.8208234136800994E-5 0.0 0.8179712102073959 5.675302828338097E-4 68-69 2.9104117068400497E-5 5.8208234136800994E-5 0.0 0.9109006560067987 5.8208234136801E-4 70-71 2.9104117068400497E-5 5.8208234136800994E-5 0.0 1.036790514386165 5.8208234136801E-4 72-73 5.8208234136800994E-5 5.8208234136800994E-5 0.0 1.1738563537197972 6.402905755048109E-4 74-75 5.8208234136800994E-5 5.8208234136800994E-5 0.0 1.3256779804071084 6.402905755048109E-4 76-77 7.276029267100123E-5 5.8208234136800994E-5 0.0 1.4993859031298569 6.402905755048109E-4 78-79 8.731235120520148E-5 5.8208234136800994E-5 0.0 1.6979632938875533 6.402905755048109E-4 80-81 8.731235120520148E-5 5.8208234136800994E-5 0.0 1.9189363027293842 6.693946925732114E-4 82-83 8.731235120520148E-5 5.8208234136800994E-5 0.0 2.1520748325058063 8.149152779152139E-4 84-85 8.731235120520148E-5 7.276029267100123E-5 0.0 2.3797708923904377 8.149152779152139E-4 86-87 8.731235120520148E-5 8.731235120520148E-5 0.0 2.616634749151615 8.294673364494141E-4 88-89 8.731235120520148E-5 8.731235120520148E-5 0.0 2.878397178064809 9.022276291204154E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 10620 0.0 39.00965 1 GTATCAA 16245 0.0 32.38832 1 ATCAACG 19890 0.0 26.15436 3 TATCAAC 20010 0.0 26.14452 2 TCAACGC 20050 0.0 25.992907 4 CAACGCA 20355 0.0 25.603428 5 AACGCAG 20835 0.0 25.05905 6 GTACATG 14015 0.0 21.848648 1 GAGTACT 12925 0.0 21.792673 12-13 ACGCAGA 23790 0.0 21.74727 7 CGCAGAG 24170 0.0 21.366156 8 GTACTTT 13365 0.0 20.968866 14-15 TACATGG 14455 0.0 20.652905 2 GCAGAGT 25000 0.0 20.05036 9 ACATGGG 14220 0.0 19.924074 3 AGTACTT 13250 0.0 19.54175 12-13 AGAGTAC 20770 0.0 19.172459 10-11 CGCGGAA 590 0.0 18.46992 82-83 ACTTTTT 15810 0.0 18.16059 16-17 CAGAGTA 24505 0.0 18.15463 10-11 >>END_MODULE