FastQCFastQC Report
Fri 27 May 2016
SRR939498_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939498_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3414827
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83220.24370195034770428No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67580.19790167993869087No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44660.13078261358481705No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA91500.035.6261251
GTATCAA142700.035.4288251
TATCAAC178350.027.968192
ATCAACG181800.027.4946233
TCAACGC182450.027.2780934
CAACGCA184700.026.7188245
AACGCAG190700.026.0096936
GTACATG120300.025.7590451
TACATGG125650.023.6634672
ACGCAGA208850.023.4011067
CGCAGAG212050.022.9360568
ACATGGG130900.022.4557553
GAGTACT105350.021.4697612-13
GCAGAGT210650.021.4317349
AGAGTAC166650.021.0230210-11
GTACTTT112600.020.33281514-15
AGTACTT110800.019.97983712-13
CATGGGG73550.018.212984
CAGAGTA204950.017.92244710-11
AACGCGG4700.017.5372456
Produced by FastQC (version 0.11.5)