FastQCFastQC Report
Fri 27 May 2016
SRR939498_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939498_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3414827
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123430.36145315707062176No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118810.34792392118253723No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74150.21714130759772016No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA95050.041.152831
GTATCAA149800.034.477971
TATCAAC189550.026.8919532
TCAACGC189850.026.8323234
ATCAACG190350.026.7991523
CAACGCA194500.026.2618965
AACGCAG198700.025.7545266
ATACCGG1000.00166908923.7137366
ACGCAGA224900.022.6698687
CGCAGAG228900.022.1701158
GTACATG135500.021.5807251
GAGTACT119550.021.51210812-13
TACATGG139650.020.5615712
GCAGAGT240150.020.4403239
GTACTTT125400.020.41404314-15
AGTACTT125550.020.18198212-13
AGAGTAC199450.019.93427310-11
ACATGGG138450.019.8107223
CAGAGTA231550.018.34824810-11
ACTTTTT152350.017.45633916-17
Produced by FastQC (version 0.11.5)