FastQCFastQC Report
Fri 27 May 2016
SRR939495_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR939495_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1598085
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81650.5109240121770745No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69250.4333311432120319No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44430.27802025549329357No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32780.20512050360274955No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24360.15243244257971258No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA21390.1338476989646984No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20710.12959260615048637No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA17450.10919319059999938No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16040.10037013049994212No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG12850.032.3910832
CAACGCG1601.5570549E-930.253535
GTATCAA141050.029.88531
TATCACG2001.6370905E-1129.3806172
GGTATCA98000.028.7270491
GTACATG110400.026.6865271
GTACCTG15400.026.4892441
TACATGG112100.025.9908832
ACCTGGG16200.025.469363
TATCAAC168900.024.8172592
ACATGGG116400.024.3595433
ATCAACG172850.023.9538863
CATGGGG39500.023.785874
TCAACGC176150.023.729794
CAACGCA177000.023.3823875
AACGCAG179550.022.7904426
GAGTACT86350.022.77648712-13
ATAACGC1502.8087441E-522.3893453
GTCCTAC25450.021.767581
CATGGGA67500.021.5888674
Produced by FastQC (version 0.11.5)